LOCUS       sequence-399769-        4489 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tardigrade (Hypsibius exemplaris) alpha-tubulin Tub1A2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4489)
  AUTHORS   Novotna Floriancicova K, Baltzis A, Smejkal J, Czernekova M, 
            Kaczmarek L, Maly J, Notredame C, Vinopal S
  TITLE     Phylogenetic and functional characterization of water bears 
            (Tardigrada) tubulins.
  JOURNAL   Sci Rep. 2023 Mar 30;13(1):5194. doi: 10.1038/s41598-023-31992-z.
  PUBMED    36997657
REFERENCE   2  (bases 1 to 4489)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2023-03-30"; volume: "13(1):5194. doi"; pages: " 
            10.1038/s41598-023-31992-z"
COMMENT     Sequence Label: pJet1.2-tHe-Tub1A2
FEATURES             Location/Qualifiers
     source          1..4489
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     305..324
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        305..323
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(join(2321..2327,2328..2345,2346..2351))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    2366..2387
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2507..2524)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2678..3266)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2758..2777)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3437..4228,4229..4297))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4060..4079
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4298..4402)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4470..4488
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gcccctgcag ccgaattata ttatttttgc caaataattt ttaacaaaag ctctgaagtc
       61 ttcttcattt aaattcttag atgatacttc atctggaaaa ttgtcccaat tagtagcatc
      121 acgctgtgag taagttctaa accatttttt tattgttgta ttatctctaa tcttactact
      181 cgatgagttt tcggtattat ctctattttt aacttggagc aggttccatt cattgttttt
      241 ttcatcatag tgaataaaat caactgcttt aacacttgtg cctgaacacc atatccatcc
      301 ggcgtaatac gactcactat agggagagcg gccgccagat cttccggatg gctcgagttt
      361 ttcagcaaga tggaaccttc tctcctgttt ctgattcctc tacgtgtttt tttcgtctag
      421 tccacacaca cacagaccga atccaaaatg cgcgaatgca tctctatcca cgtcggacag
      481 gccggagtgc agattggcaa cgcctgctgg gagctttact gtctggagca tggcatccag
      541 ccggacgggc agatgccgtc ggacaagacg gttggcgccg ctgacgactc cttcaacacc
      601 ttcttcagcg agaccggatc cggcaagcac gttccgcggg ccgtcttcgt cgatctcgag
      661 ccgaccgttg ttgatgaagt gcgaaccgga acataccgcc agcttttcca tccagagcag
      721 ctaatcaccg gaaaggagga tgccgccaac aattacgctc gcgggcacta caccgtcggc
      781 aaggagattg tcgacctggt gctggaccgc gtgcgcaagc tcgccgacca atgtaccggt
      841 ctgcagggct ttctcatctt tcactcgttc ggcggtggga ccggatccgg attcacctcc
      901 ctgctgatgg aacgcttgtc ggtcgactac gggaagaagt ccaaactgga attctccatc
      961 taccccgccc ctcaggtgtc gactgccgtg gtggagccgt acaactcgat cctgacgacc
     1021 cataccacgc tggagcactc tgactgtgcc ttcatggtga acaacgaagc catctacgac
     1081 atttgccgtc ggaaccttga cattgagcgt ccgacctaca ccaacctcaa ccgcctgatc
     1141 gggcagattg tctcctccat caccgcgtcg ctgcgcttcg acggcgctct caacgtcgat
     1201 ctcaccgaat tccagaccaa cttggttcct tacccccgta tccatttccc gctggtgacc
     1261 tacgccccga tcatttccgc tgaaaaggcc tatcacgaac agttgacggt ctcggagatc
     1321 accaactcgt gcttcgagcc ggctaaccag atggtcaagt gcgacccgcg ccacggaaag
     1381 tacatggcct gctgcatgct ctaccgcggt gacgttgtgc ccaaggacgt gaacgccgcc
     1441 atcgcggcca ttaagaccaa gcgcagcatc cagtttgtcg actggtgccc gactggattt
     1501 aaggtgggaa tcaactatca gcccccgacg gtggtgccgg gcggggattt ggccaaggtg
     1561 cagcgtgccg tctgcatgct gtccaacacc acggccatcg ccgaagcctg ggcgcgtctg
     1621 gaccacaagt tcgacctgat gtacgccaag cgcgcctttg tccactggta cgtcggtgag
     1681 ggcatggagg agggcgagtt cagcgaggcc cgtgaagatt tggccgccct cgagaaggac
     1741 tacgaggagg tcggtgtgga ctctgtcgac gctggtgagg aaggcgagga atattgagac
     1801 cactcctctc gtttcccttc tttaatctct ggcccttttt tctctcgctc gatacacttt
     1861 gactcgggaa tctctcgtcc ttaaacatct ttctagaaga tctcctacaa tattctcagc
     1921 tgccatggaa aatcgatgtt cttcttttat tctctcaaga ttttcaggct gtatattaaa
     1981 acttatatta agaactatgc taaccacctc atcaggaacc gttgtaggtg gcgtgggttt
     2041 tcttggcaat cgactctcat gaaaactacg agctaaatat tcaatatgtt cctcttgacc
     2101 aactttattc tgcatttttt ttgaacgagg tttagagcaa gcttcaggaa actgagacag
     2161 gaattttatt aaaaatttaa attttgaaga aagttcaggg ttaatagcat ccattttttg
     2221 ctttgcaagt tcctcagcat tcttaacaaa agacgtctct tttgacatgt ttaaagttta
     2281 aacctcctgt gtgaaattat tatccgctca taattccaca cattatacga gccggaagca
     2341 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     2401 cactgccaat tgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     2461 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     2521 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     2581 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     2641 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     2701 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     2761 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     2821 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     2881 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     2941 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     3001 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     3061 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     3121 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     3181 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     3241 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     3301 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     3361 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     3421 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     3481 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     3541 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     3601 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     3661 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     3721 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     3781 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     3841 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     3901 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     3961 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     4021 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     4081 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     4141 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     4201 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     4261 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     4321 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     4381 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
     4441 ccattattat catgacatta acctataaaa ataggcgtat cacgaggcc
//
