LOCUS       sequence-400361-       14083 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14083)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 14083)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1605
FEATURES             Location/Qualifiers
     source          1..14083
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(5141..5152)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    9940..10528
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9993..10013)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10411..10422)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10600..10833
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10911..11032
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10948..10967)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11002..11021
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      11072..11207
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(11227..11246)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11228..11246)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11256..11273)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11256..11272)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11265..11287)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      11414..11869
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11501..11520)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11711..11732
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        11895..11999
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12000..12068,12069..12860)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12218..12237)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      13031..13619
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13520..13539
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13773..13790
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    13907..13928
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13943..13948,13949..13966,13967..13973)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13981..13997
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13986..14008
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     14005..14021
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     14005..14021
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     14040..14060
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        14042..14060
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggaa atggaggcaa acgaccattt
     4141 taactttact ggccttcccc ctgcacctgc tgcctcagga ctgaaaccct ctccttcctc
     4201 aggggagggc ctctacacta acgggtctcc catgaacttc ccccagcaag ggaaaagttt
     4261 gaatggggat gtgaatgtta atggcttatc tactgtatct cacactacta cttcagggat
     4321 tttgaactct gctccccact cctccagcac ctcacacctc catcacccca gcgtggccta
     4381 cgactgtctc tggaactact cacagtaccc atctgccaat cctggcagca acctcaagga
     4441 cccacccctt ctctcccagt tctcgggggg acaataccca ctcaacggca tccttggggg
     4501 cagccggcaa ccttcatccc caagtcataa cactaacctt cgggctggga gccaagagtt
     4561 ctgggccaac ggtacccaga gtcccatggg gcttaacttt gattcacaag aactgtatga
     4621 ttcctttcct gaccagaatt ttgaggtgat gcccaatgga ccccctagtt ttttcacctc
     4681 cccacagact tctcctatgt tgggatctag cattcaaacc tttgcaccct cccaggaggt
     4741 aggcagtggt atccatcctg atgaggcagc agaaaaggag atgacttcag ttgtggcaga
     4801 gaatggcact ggcttggtag gcagcttgga gctggaagaa gagcagccag aactgaagat
     4861 gtgtggctac aatggctctg tcccttctgt ggaatcgtta caccaagagg tctcagtcct
     4921 ggtccctgac cccacagtga gctgtttaga tgatccttca catcttcctg atcaactgga
     4981 agacactcca atcctcagtg aagactctct ggagcccttc aactctctgg caccagagcc
     5041 agtgagtgga ggactatatg gtattgatga cacggagctg atgggtgcag aggacaagct
     5101 gcctcttgag gacagccctg tgatttctgc ccttgattgc ccttccctca ataatgctac
     5161 tgccttcagt ctcctggcag atgatagtca aacatcaacc tctatctttg ccagtcccac
     5221 ctctccacct gtcctagggg agtctgtcct gcaagataac agctttgacc tgaataatgg
     5281 tagtgacgct gaacaggaag aaatggaaac tcaatcttca gacttcccac catccctgac
     5341 ccagccagct cctgatcagt catccactat tcagctacat ccagcaacct caccagcagt
     5401 ctcgccaaca acctccccag cagtctccct agtggtttct ccagcagcct ccccagaaat
     5461 ctctccagaa gtttgtcccg cagcttctac agttgtctct ccagcagtct tctcagtggt
     5521 ctctccagct tcctcagcag tcctcccagc agtctcctta gaagtcccgt tgacggcttc
     5581 agtgacatcc ccaaaagcct ctcccgtaac ttccccagca gctgcctttc caacagcctc
     5641 cccagcaaat aaggatgtca gcagctttct agaaaccact gctgacgtgg aagagatcac
     5701 tggagaagga ctcactgctt ctggtagtgg tgatgtcatg aggagacgta ttgctacccc
     5761 agaagaagtt cgtcttcccc tccaacatgg gtggcggaga gaggtgcgca tcaagaaggg
     5821 cagccaccga tggcaggggg agacctggta ttatggcccc tgtgggaaga ggatgaagca
     5881 atttccagaa gtgatcaagt acctgagccg caacgtggta cacagtgtcc gccgagagca
     5941 cttcagcttc agtccccgta tgcctgttgg agatttcttt gaagaaagag acacgccaga
     6001 gggcttgcag tgggtgcagc tctcagcaga ggagatcccg tcgaggattc aggcaattac
     6061 tggcaaacgg ggtcgacctc gaaacactga gaaggctaag actaaggaag tccccaaggt
     6121 gaaacggggt cgaggtcggc cacctaaggt caaaatcact gagctattga acaagacaga
     6181 caaccgcccc ctaaagaaac tggaggccca agaaacattg aatgaggagg ataaagcaaa
     6241 gattgctaaa agcaagaaga agatgaggca gaaggttcaa cggggagagt gtcagactac
     6301 tatccaaggg caggccagaa ataagcggaa acaagagacc aagagcttaa agcagaagga
     6361 agctaagaag aaatccaagg ctgagaaaga aaaaggaaag acaaagcagg aaaaactgaa
     6421 ggaaaaagtc aagagggaaa agaaggagaa ggtaaaaatg aaggaaaagg aggaggtgac
     6481 caaagccaag ccagcctgta aagcagataa gaccctggcc acacagaggc gcttggagga
     6541 acggcagagg cagcagatga tcttggagga aatgaagaag ccgacagagg atatgtgtct
     6601 gactgaccac cagcccctgc ctgacttctc acgagtccct ggtctgacat tgcccagtgg
     6661 agccttctca gactgcttga ccattgtgga gttcctgcat agctttggca aggtgctggg
     6721 ctttgatcct gccaaagatg tgcctagcct gggggtcctg caggagggac tcctgtgtca
     6781 aggtgacagc ttgggtgagg tgcaagacct gctggtcagg ctgctgaagg ctgcactcca
     6841 tgatcctggc tttccctcct actgtcagtc cctaaagatc ttgggggaga aggtgtctga
     6901 gatcccactg acaagagaca atgtgtcaga gatcctgcgc tgcttcctta tggcatatgg
     6961 agtagagcca gccctctgtg accgcctgcg cacccagcct tttcaggccc agccacccca
     7021 gcagaaggct gctgtcctgg ccttccttgt gcatgagctc aatggctcca ccctcatcat
     7081 caatgagatt gacaagactc tggagagtat gtccagctac aggaaaaaca agtggattgt
     7141 tgaaggccgg ctccggaggc tgaaaactgt tctggccaag cgaactgggc ggtctgaagt
     7201 agagatggaa gggccagagg aatgcctggg acggaggcgc agttctcgga tcatggagga
     7261 gaccagtggc atggaagaag aggaagaaga ggagtctata gcagctgtcc ctggccgcag
     7321 gggtcgaaga gatggagagg ttgatgccac agcatctagc atcccagagc tagagcgcca
     7381 gatagaaaaa ctcagcaagc gtcagctttt ctttcgcaaa aagctgcttc actcatccca
     7441 gatgcttcgg gcggtctccc tgggtcagga ccgctacaga cgtcgctact gggtattgcc
     7501 gtatttggct ggtatctttg tagaaggaac agaggggaac ttagttcctg aggaggtgat
     7561 aaagaaggaa actgactcct taaaagtggc agcccatgcg tcactcaacc ctgccctctt
     7621 ctctatgaag atggagttag ctggctccaa caccactgcc agttctcctg cccgggcccg
     7681 aggccgacct cgaaaaacta agcccgggtc tatgcaacct aggcatctta agtcccctgt
     7741 caggggtcag gattcagaac agccccaggc ccagcttcag cctgaggctc agcttcatgc
     7801 tcctgcccag ccccagcctc agcttcagct tcagcttcag tcccataagg ggttcctgga
     7861 gcaagaaggc tcccctttgt cactgggtca gagccagcat gacctcagcc agtcagcctt
     7921 cctgtcttgg ctgagccaga ctcagagcca tagctccctg ttgagcagct cagtcctcac
     7981 acctgatagc agtccgggaa aactagaccc agctccatca caacccccgg aggagccaga
     8041 gcctgatgag gcagaatcca gccctgatcc tcaagcactc tggtttaaca tctcagccca
     8101 gatgccctgc aatgctgccc ccacaccgcc ccctgcagtt tctgaggacc aacccactcc
     8161 ctcccctcag cagcttgcct cctccaagcc aatgaataga cctagtgctg ccaacccttg
     8221 ttctccagtg cagttctctt ccacgccctt ggctgggttg gcccctaaga ggcgagcagg
     8281 agaccctgga gaaatgccac agagtcccac agggctggga cagcccaaac ggagagggag
     8341 acctcccagt aagttcttca aacagatgga acagcgttac ctaacccagc tgacagccca
     8401 gcctgtccca cctgagatgt gctcaggctg gtggtggata cgagatcctg agatgttgga
     8461 tgccatgctc aaggccctac acccccgagg tatccgggag aaggcacttc acaaacacct
     8521 taacaagcac agggacttct tgcaggaagt ctgcctgcgg ccctcagctg accccatctt
     8581 tgagcccagg caactacctg cctttcaaga agggattatg agctggtccc ccaaagagaa
     8641 gacatacgag acagacctag cagtgcttca atgggtagag gagctggagc agcgggttat
     8701 catgtctgat ctgcagattc ggggctggac atgtcctagc ccagactcta cccgtgaaga
     8761 cttggcctac tgtgagcacc tctccgactc ccaggaggat atcacctggc gaggtcgggg
     8821 cagggaggga ctggcacctc agcgtaaaac taccaaccct ttggacctgg ctgtgatgcg
     8881 gctggctgcc ctggaacaga atgtagaacg gcggtacctg cgggagcccc tctggccaac
     8941 tcatgaggtt gtgctggaga aggccctgct tagcacacct aatggtgccc ctgagggcac
     9001 cactacagag atatcatatg agatcacccc tcgcattcgt gtctggcgcc agaccctcga
     9061 gcggtgccgg agcgcagccc aggtgtgctt gtgcctgggc cagctggaga ggtccattgc
     9121 ctgggagaag tctgtcaaca aagtgacatg tctagtctgc cggaagggtg acaatgatga
     9181 gtttcttctg ctttgtgatg ggtgtgaccg tggctgccac atttactgcc atcgtcccaa
     9241 gatggaggct gtcccagaag gagattggtt ctgtactgtc tgtttggctc agcaggtgga
     9301 gggagaattc actcagaagc ctggtttccc aaagcgtggc cagaagcgga aaagtggtta
     9361 ttcgctgaac ttctcagagg gtgatggccg ccgacgccgg gtactgttga ggggccgaga
     9421 aagcccagca gcagggcctc ggtactcgga agaagggctc tccccctcca agcggcggcg
     9481 actctctatg cggaaccacc acagtgatct cacattttgc gagattatcc tgatggagat
     9541 ggagtcccat gatgcagcct ggcctttcct agagcctgtg aacccacgtt tggtgagtgg
     9601 gtaccggcgc atcatcaaaa atcctatgga tttttccacc atgcgggagc ggctgctcag
     9661 gggagggtac accagctcag aggagtttgc ggctgatgcc ctcctggtat ttgacaactg
     9721 ccagactttc aacgaggatg actctgaagt aggcaaggct gggcacatca tgcgccgctt
     9781 cttcgagagc cgctgggagg agttttatca gggaaaacag gccaatctgt agtaatgaac
     9841 tagtccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga
     9901 tccttgtaag ctgagtttgt aaatacgcgt taagtcgaca atcaacctct ggattacaaa
     9961 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
    10021 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
    10081 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
    10141 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
    10201 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
    10261 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
    10321 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
    10381 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
    10441 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
    10501 cggatctccc tttgggccgc ctccccgcgt cgactttaag accaatgact tacaaggcag
    10561 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
    10621 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
    10681 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
    10741 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
    10801 gaccctttta gtcagtgtgg aaaatctcta gcagtacgta tagtagttca tgtcatctta
    10861 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta
    10921 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
    10981 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
    11041 ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
    11101 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
    11161 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc
    11221 aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt
    11281 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
    11341 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
    11401 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
    11461 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
    11521 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
    11581 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
    11641 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
    11701 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
    11761 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
    11821 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt
    11881 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
    11941 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
    12001 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
    12061 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
    12121 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
    12181 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
    12241 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
    12301 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
    12361 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
    12421 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
    12481 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
    12541 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
    12601 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
    12661 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
    12721 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
    12781 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
    12841 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
    12901 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
    12961 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
    13021 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
    13081 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
    13141 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
    13201 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
    13261 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
    13321 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
    13381 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
    13441 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
    13501 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
    13561 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
    13621 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
    13681 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
    13741 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
    13801 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
    13861 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
    13921 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
    13981 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
    14041 caattaaccc tcactaaagg gaacaaaagc tggagctgca agc
//
