LOCUS       sequence-400545-       16084 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16084)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 16084)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2476
FEATURES             Location/Qualifiers
     source          1..16084
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    11941..12529
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(11994..12014)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(12412..12423)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             12601..12834
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    12912..13033
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12949..12968)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     13003..13022
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      13073..13208
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(13228..13247)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(13229..13247)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(13257..13274)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(13257..13273)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13266..13288)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      13415..13870
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(13502..13521)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     13712..13733
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        13896..14000
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(14001..14069,14070..14861)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(14219..14238)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      15032..15620
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     15521..15540
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     15774..15791
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    15908..15929
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(15944..15949,15950..15967,15968..15974)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    15982..15998
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     15987..16009
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     16006..16022
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     16006..16022
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     16041..16061
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        16043..16061
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggcc acccttaact cagcctctac
     4141 cactggtacc accccctccc ctgggcacaa tgccccgtcc ctgccttcgg acaccttctc
     4201 ctccagcacc ccctctgatc ctgtcaccaa agatccccct gctgcctcct ccacctctga
     4261 gaacatgagg tcctcagagc cagggggaca gctcctggag tcgggctgtg gcctcgtccc
     4321 accaaaggag attggggagc cccaggaagg gcctgactgt ggtcacttcc caccaaatga
     4381 cccaggggtg gaaaaggaca aggagcagga ggaggaagaa gaagggctcc ctcccatgga
     4441 cctaagcaac cacttattct tcacagctgg aggtgaggcc tacctagtgg ccaagctgtc
     4501 cctgccaggt ggcagtgaac tcctgttacc aaagggcttc ccctggggtg aggcgggcat
     4561 caaggaagag cccagtctgc ccttccttgc ctacccaccc ccctcacacc tcactgccct
     4621 tcacatccaa catggctttg acccaatcca aggctttagc tcttctgacc aaattctgtc
     4681 ccatgatacc tcagcaccat ctccggctgc ctgtgaggaa aggcatggag ctttctggag
     4741 ctaccagctg gctccaaatc cacccggaga tcccaaagat ggccccatgg ggaacagcgg
     4801 gggcaaccac gtggcggtct tctggctctg ccttctgtgc cgcctgggtt tcagcaagcc
     4861 ccaggccttt atggatcaca cacagtctca tggggtgaag ctaacccctg cccaatatca
     4921 gggcctgtca ggtagcccag ctgtactcca ggagggagat gaaggctgca aggccctcat
     4981 aagctttctg gagccaaaac tccctgctcg cccctcttct gacatacccc ttgacaacag
     5041 cagcacagtg aacatggagg cgaatgtggc ccagacagag gatggccccc ctgaggcaga
     5101 agtccaggcc cttatcctcc tggatgaaga agttatggcc ctcagcccac cctctccacc
     5161 cacagccacc tgggacccca gcccaaccca agccaaagaa tcgccagtag cagcaggcga
     5221 ggcagggcca gattggttcc ctgaggggca agaagaggat ggagggctct gccccccact
     5281 caaccaaagc tcacccacct ccaaggaggg gggcactctc cctgccccag tgggctcccc
     5341 cgaagacccc agtgacccac cccagcccta tcgcctagct gatgactaca ccccagcccc
     5401 tgcagccttc cagggcctca gcctgtccag ccacatgtcc ctgctccact cacgcaactc
     5461 ctgcaagaca ctcaagtgtc ccaagtgcaa ctggcactac aagtaccagc agaccctgga
     5521 tgtgcacatg cgagagaagc accctgagag caacagtcac tgcagctact gcagtgctgg
     5581 gggcgcccac ccccgccttg ctcgtggaga gagctacaac tgtggctaca aaccctaccg
     5641 ctgtgacgtc tgcaactact ctacaaccac caaaggcaac ctcagcatcc atatgcagtc
     5701 tgacaagcac ctggccaacc tacagggctt ccaggcgggc cctggtgggc agggaagtcc
     5761 accagaggca tcactcccac cctccgcggg agacaaagag cctaagacca aatcatcctg
     5821 gcagtgcaag gtgtgcagct acgagacaaa catctcccgt aacctgcgca tccatatgac
     5881 ctctgagaag cacatgcaga atgtcctaat gctgcaccag gggctgccgc tgggcctgcc
     5941 acctggattg atggggccag gccctcctcc cccaccaggg gctaccccca ctagcccccc
     6001 tgaactcttc cagtactttg ggccccaggc cctagggcag cctcagactc ccttggctgg
     6061 cccggggctg aggccagaca agcccctgga agcccagcta cttctcaatg gtttccacca
     6121 cgtaggagca cctgcccgca agttccccac atccgcccct ggaagcctat cccctgatgc
     6181 ccacctgcct ccaagtcagc tcctgggttc ctcatctgac agcctgccca cctcaccacc
     6241 cccagacgac agcctgtccc tgaaggtgtt ccgctgccta gtgtgccagg ccttcagcac
     6301 agacagcctg gagctgctgc tctaccactg cagcataggc cggagcctcc cggaagctga
     6361 atggaaggag gtggctggtg acacccaccg ctgcaagctt tgctgctatg gcacccagct
     6421 caaggccaac ttccaactcc acctcaagac tgacaaacat gctcagaagt accagctggc
     6481 agcccacctg cgggaggggg gtggagccat gggcacccct tccccagcat ccctgggaga
     6541 tggggctcct tatgggtctg tctccccact acacctgcgc tgcaacatct gtgactttga
     6601 gtccaacagc aaggagaaga tgcagctgca tgccaggggt gcagcccacg aagaaaacag
     6661 ccaaatctat aagtttctgc tggacatgga gggagcggag gcaggggcag agctggggct
     6721 ataccactgc ctgttgtgtg cgtgggagac accctcccgc ttggctgtgc tgcaacacct
     6781 gcgcacacct gcccaccgcg atgcccaggc ccagaggcgt ctgcagctgc tacagaatgg
     6841 cccaaccact gaggaaggac tcgcagctct tcagagcatc ctgagcttca gccacgggca
     6901 gctccggact cccgggaagg ctcctgtcac ccccttagct gagccaccca cccctgagaa
     6961 agatgcccag aacaagacag aacaattggc ttcagaagag acagaaaaca agactggccc
     7021 ttccagagac agtgccaacc agaccacggt atactgctgt ccatactgca gcttcctgag
     7081 cccagagtcc agccaggtga gggctcatac actctcccag catgcagtgc agcccaagta
     7141 cagatgccca ctgtgccagg aacagctggt gggccggcct gccctgcact tccaccttag
     7201 ccaccttcac aacgtggtgc ccgagtgcgt tgagaagctg ctgcttgtag ctacaactgt
     7261 agaaatgaca tttacaacca aagtgctgtc tgcacccaca ttaagccctc tggacaatgg
     7321 ccaagaaccc cccactcatg ggccagagcc tacaccgagc agagaccagg cagcagaagg
     7381 ccctaacctg accccagaag ccagtccaga tcctcttcct gagcctcccc tggcctcagt
     7441 tgaggtccca gacaaaccct caggaagccc tggccaaccc ccttctccag ccccatctcc
     7501 agtccctgaa cctgatgccc aagctgaaga cgtagctcct ccgcccacca tggctgagga
     7561 ggaagagggg accactgggg agctccgctc tgcagagcca gctccagctg actctcgcca
     7621 ccctctgacc tatcggaaaa ccaccaactt tgccctggac aagtttctcg accctgcccg
     7681 gccctataag tgcactgtgt gtaaggagtc cttcacccag aagaatattc tgttggttca
     7741 ttataattct gtctcccacc ttcacaagat gaagaaggct gccattgacc cctctgcccc
     7801 tgcacgggga gaggccggtg ccccacccac caccactgct gccacagaca agccctttaa
     7861 gtgcacagtc tgcagagtct cctacaacca gagctccacc ctggagatcc acatgcggtc
     7921 agttctgcat cagactcgct ctcggggaac caagactgat tccaagattg aagggccaga
     7981 acgcagccaa gaagagccca aggaaggcga gacagagggg gaggtgggca ctgagaagaa
     8041 gggccctgac accagtggct tcatatctgg attgcctttc ctgtcccctc ccccacctcc
     8101 cttggacctg caccgattcc cagcccctct cttcacccca ccagtcctgc cccccttccc
     8161 tctggtgccc gaatcactgc ttaagctcca gcagcagcag ctgctcctgc ccttctacct
     8221 ccacgatctc aaggtggggc ccaagctgac actagctggg cctgcacctg tgctgtccct
     8281 gccagctgcc acccctcctc ctccacccca acctcccaag gctgagctgg ctgagcggga
     8341 gtgggagcgg ccccccatgg ccaaagaggg taatgaggca gggccttcct caccccccga
     8401 cccattgccc aacgaggctg cccgcactgc agccaaagcc cttctagaaa actttggctt
     8461 tgagctggtg atccagtaca atgaagggaa gcaagctgtg ccccctcccc ctaccccacc
     8521 cccacctgag gccctcgggg gtggggacaa gctggcctgt ggggcctgtg ggaaactctt
     8581 ctccaatatg cttatcctca agacacacga ggaacatgtc caccgccgct ttctgccctt
     8641 tgaagccctg agccgttatg ctgctcagtt tcgaaagagc tatgacagcc tatacccgcc
     8701 ccttgcagag cctcccaaac ctcctgatgg gtctctggat tcacctgttc cccatctggg
     8761 cccacccttc ctggtcccag agcctgaggc aggggggacc cgtgcccctg aagagcgaag
     8821 tcgagcaggg ggacactggc ccatagaaga ggaagaaagc tccagaggga atcttcctcc
     8881 cctggtgcct gccggccgcc ggttctccag aaccaagttc acagagttcc agacccaagc
     8941 cctgcagtct ttctttgaga ctagcgccta ccccaaagac ggagaggtgg agcgactcgc
     9001 aagtctgttg ggtctggcta gccgtgtggt ggtggtgtgg ttccagaatg cccgccagaa
     9061 agcacgcaaa aatgcctgtg agggtgggtc catgccaacc ggaggaggca ctgggggagc
     9121 ctccggctgc aggcgttgcc acgccacttt ctcctgtgtt tttgagttgg tgcgccacct
     9181 caagaagtgc tatgatgacc agacccttga agaggaggag gaagaggcag agagagggga
     9241 agaggaggaa gaggtggaag aagaagaagt agaggaggaa cagggccttg aacctccagc
     9301 agggcctgag ggcccattac cagagcctcc agatggggag gagctgagcc aagcagaggc
     9361 aacaaaggca ggaggcaaag agcctgaaga gaaggctact ccatcacctt ccccagccca
     9421 tacctgtgac cagtgtgcca tttctttctc cagccaggac ctcctgacca gtcaccgccg
     9481 actacatttc ctgccatctc tgcagcccag tgctcccccc caactcctag atctgccctt
     9541 gctggtgttt ggggagagaa accccctggt ggcagccacc tcaccaatgc caggtccacc
     9601 tctcaaacgg aagcatgagg acggcagctt gtctcccaca ggcagtgaag cagggggagg
     9661 aggggagggc gagcccccca gggacaagcg cctgcgcacc accatcttgc ctgagcagct
     9721 agagatcctg taccgttggt acatgcagga ttccaaccca acacgcaaga tgctcgactg
     9781 catctccgag gaggtggggc tcaaaaagcg agtggtacag gtctggttcc agaataccag
     9841 ggcccgggag aggaaaggcc agtttcgaag cacccctggg ggggtgccta gtccagcagt
     9901 gaaaccgcct gccacagcca cccctgcatc cttgcccaag ttcaacctct tattaggcaa
     9961 ggtagatgat ggcactggga gggaagcccc aaagagggaa gcacctgctt ttccctaccc
    10021 cactgccacg cttgcttctg ggccccagcc tttcctacca cctgggaaag aggccaccac
    10081 cccaacacca gagccacctc tacctctcct acctccccct ccacccagtg aggaagaggg
    10141 cccagaggaa ccacctaaag cttctccaga gagtgaggct tgcagtctct ctgcaggaga
    10201 tctgagtgat tcatctgctt ccagcctagc tgaaccagaa tcccctgggg ctggagggac
    10261 cagtggggga cctggaggtg ggactggggt tccagatgga atggggcagc ggcgctacag
    10321 gacccagatg agcagcctgc agctgaagat catgaaagcc tgctatgaag cttaccgcac
    10381 ccccaccatg caggagtgtg aggtgctggg agaggagatt gggctgccca agagagtcat
    10441 ccaggtctgg ttccagaatg ctcgtgccaa ggaaaagaag gccaaactac aggggacagc
    10501 cgctgggagc actgggggca gcagtgaggg cctcttagca gcccagcgca ctgactgccc
    10561 ctattgtgat gtcaagtatg atttctatgt ctcctgccga ggccatctct tttcccgtca
    10621 gcacctggcc aagctcaagg aggcggttcg agcccagctg aagagtgaaa gcaagtgcta
    10681 cgacttggcc ccagcacctg aggctccccc agctctcaag gccccacctg ccaccacacc
    10741 tgcctccatg cccctcgggg ctgccccaac cttgcctcgc ctggcgccgg tcctcttatc
    10801 tggcccagct ctggctcagc ccccgctggg caacttagct cctttcaatt caggcccggc
    10861 agcctcctca ggcctcctcg gcctcgccac ttcggtcctg cctaccacca cagtggtcca
    10921 gactgctggc ccaggccgcc ccttacctca gagacccatg cccgaccaaa ccaacacctc
    10981 cacagcaggc accactgacc ctgtcccagg ccctcctact gagcccttgg gggacaaggt
    11041 ctccagtgag cgaaagccag ttgcaggccc caccagctcc tccaatgatg ccctcaagaa
    11101 cctcaaagca ttgaagacca ctgtcccagc cctgttgggg ggccagttcc tgccctttcc
    11161 attgccccct gctgggggaa cagcaccgcc agctgtcttt ggcccccagc tacagggggc
    11221 ctacttccaa cagctctatg gcatgaagaa ggggctattt cccatgaacc ccatgatacc
    11281 tcagaccctc attgggctgc tccccaatgc cctcctccag ccgccacccc agccccctga
    11341 gcccacagcc acagcacctc caaagcctcc tgaactgcct gctccagggg agggggaagc
    11401 tggtgaggtt gatgagctgc tgacaggcag cactggcatc tccaccgtgg atgtaaccca
    11461 tcgctacctg tgccgccagt gcaagatggc atttgacggg gaggccccgg ctactgccca
    11521 ccagagatcc ttctgcttct ttgggcgggg ctctgggggc tccatgccac ccccattgcg
    11581 ggtgcccatc tgcacctacc actgcctggc atgtgaggtg ctgctgagtg ggcgtgaagc
    11641 cctagcctcc cacctgcgct cctcggccca caggcgcaag gcagccccac ctcaaggggg
    11701 cccacccatc tccatcacca acgccgccac tgctgcctcg gctgctgtgg cttttgccaa
    11761 agaggaagca agattacctc acacggactc caacccaaaa actacgacta cctctacact
    11821 tctagcttta tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta
    11881 tatatcttgt ggaaaggacg agggtgcctg cttccttgca acctaacgcg ttaagtcgac
    11941 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
    12001 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
    12061 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
    12121 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
    12181 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
    12241 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
    12301 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
    12361 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
    12421 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
    12481 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcg tcgactttaa
    12541 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
    12601 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
    12661 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
    12721 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
    12781 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtacgt
    12841 atagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
    12901 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
    12961 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
    13021 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc
    13081 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
    13141 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
    13201 tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
    13261 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
    13321 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
    13381 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc
    13441 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
    13501 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
    13561 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
    13621 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
    13681 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
    13741 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
    13801 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
    13861 cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
    13921 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
    13981 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
    14041 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
    14101 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
    14161 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
    14221 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
    14281 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
    14341 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
    14401 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
    14461 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
    14521 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
    14581 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
    14641 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
    14701 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
    14761 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
    14821 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
    14881 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
    14941 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
    15001 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
    15061 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
    15121 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
    15181 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
    15241 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
    15301 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
    15361 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
    15421 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
    15481 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
    15541 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
    15601 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
    15661 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
    15721 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
    15781 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
    15841 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
    15901 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
    15961 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
    16021 accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc
    16081 aagc
//
