LOCUS       sequence-400559-       15076 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15076)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 15076)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2429
FEATURES             Location/Qualifiers
     source          1..15076
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4364..4381
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             4385..4402
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(5363..5374)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    10933..11521
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10986..11006)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(11404..11415)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             11593..11826
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    11904..12025
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11941..11960)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11995..12014
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      12065..12200
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(12220..12239)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(12221..12239)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(12249..12266)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(12249..12265)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12258..12280)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      12407..12862
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12494..12513)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12704..12725
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        12888..12992
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12993..13061,13062..13853)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(13211..13230)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      14024..14612
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     14513..14532
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     14766..14783
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    14900..14921
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(14936..14941,14942..14959,14960..14966)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14974..14990
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     14979..15001
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     14998..15014
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     14998..15014
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     15033..15053
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        15035..15053
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggcc cataacgcgg gcgccgcggc
     4141 cgccgccggc acccacagcg ccaagagcgg cggctccgag gcggctctca aggagggtgg
     4201 aagcgccgcc gcgctgtcct cctcctcctc ctcctccgcg gcggcagcgg cggcatcctc
     4261 ttcctcctcg tcgggcccgg gctcggccat ggagacgggg ctgctcccca accacaaact
     4321 gaaaaccgtt ggcgaagccc ccgccgcgcc gccccaccag cagcaccacc accaccacca
     4381 tgcccaccac caccaccacc atgcccacca cctccaccac caccacgcac tacagcagca
     4441 gctaaaccag ttccagcagc agcagcagca gcagcaacag cagcagcagc agcagcagca
     4501 acagcaacat cccatttcca acaacaacag cttgggcggc gcgggcggcg gcgcgcctca
     4561 gcccggcccc gacatggagc agccgcaaca tggaggcgcc aaggacagtg ctgcgggcgg
     4621 ccaggccgac cccccgggcc cgccgctgct gagcaagccg ggcgacgagg acgacgcgcc
     4681 gcccaagatg ggggagccgg cgggcggccg ctacgagcac ccgggcttgg gcgccctggg
     4741 cacgcagcag ccgccggtcg ccgtgcccgg gggcggcggc ggcccggcgg ccgtcccgga
     4801 gtttaataat tactatggca gcgctgcccc tgcgagcggc ggccccggcg gccgcgctgg
     4861 gccttgcttt gatcaacatg gcggacaaca aagccccggg atggggatga tgcactccgc
     4921 ctccgccgcc gccgccgggg cccccggcag catggacccc ctgcagaact cccacgaagg
     4981 gtaccccaac agccagtgca accattatcc gggctacagc cggcccggcg cgggcggcgg
     5041 cggcggcggc ggcggcggag gaggaggagg cagcggagga ggaggaggag gaggaggagc
     5101 aggagcagga ggagcaggag cgggagctgt ggcggcggcg gccgcggcgg cggcggcagc
     5161 agcaggaggc ggcggcggcg gcggctatgg gggctcgtcc gcggggtacg gggtgctgag
     5221 ctccccccgg cagcagggcg gcggcatgat gatgggcccc gggggcggcg gggccgcgag
     5281 cctcagcaag gcggccgccg gctcggcggc ggggggcttc cagcgcttcg ccggccagaa
     5341 ccagcacccg tcgggggcca ccccgaccct caatcagctg ctcacctcgc ccagccccat
     5401 gatgcggagc tacggcggca gctaccccga gtacagcagc cccagcgcgc cgccgccgcc
     5461 gccgtcgcag ccccagtccc aggcggcggc ggcgggggcg gcggcgggcg gccagcaggc
     5521 ggccgcgggc atgggcttgg gcaaggacat gggcgcccag tacgccgctg ccagcccggc
     5581 ctgggcggcc gcgcaacaaa ggagtcaccc ggcgatgagc cccggcaccc ccggaccgac
     5641 catgggcaga tcccagggca gcccaatgga tccaatggtg atgaagagac ctcagttgta
     5701 tggcatgggc agtaaccctc attctcagcc tcagcagagc agtccgtacc caggaggttc
     5761 ctatggccct ccaggcccac agcggtatcc aattggcatc cagggtcgga ctcccggggc
     5821 catggccgga atgcagtacc ctcagcagca gatgccacct cagtatggac agcaaggtgt
     5881 gagtggttac tgccagcagg gccaacagcc atattacagc cagcagccgc agcccccgca
     5941 cctcccaccc caggcgcagt atctgccgtc ccagtcccag cagaggtacc agccgcagca
     6001 ggacatgtct caggaaggct atggaactag atctcaacct cctctggccc ccggaaaacc
     6061 taaccatgaa gacttgaact taatacagca agaaagacca tcaagtttac cagatctgtc
     6121 tggctccatt gatgacctcc ccacgggaac ggaagcaact ttgagctcag cagtcagtgc
     6181 atccgggtcc acgagcagcc aaggggatca gagcaacccg gcgcagtcgc ctttctcccc
     6241 acatgcgtcc cctcatctct ccagcatccc ggggggccca tctccctctc ctgttggctc
     6301 tcctgtagga agcaaccagt ctcgatctgg cccaatctct cctgcaagta tcccaggtag
     6361 tcagatgcct ccgcagccac ccgggagcca gtcagaatcc agttcccatc ccgccttgag
     6421 ccagtcacca atgccacagg aaagaggttt tatggcaggc acacaaagaa accctcagat
     6481 ggctcagtat ggacctcaac agacaggacc atccatgtcg cctcatcctt ctcctggggg
     6541 ccagatgcat gctggaatca gtagctttca gcagagtaac tcaagtggga cttacggtcc
     6601 acagatgagc cagtatggac cacaaggtaa ctactccaga cccccagcgt atagtggggt
     6661 gcccagtgca agctacagcg gcccagggcc cggtatgggt atcagtgcca acaaccagat
     6721 gcatggacaa gggccaagcc agccatgtgg tgctgtgccc ctgggacgaa tgccatcagc
     6781 tgggatgcag aacagaccat ttcctggaaa tatgagcagc atgaccccca gttctcctgg
     6841 catgtctcag cagggagggc caggaatggg gccgccaatg ccaactgtga accgtaaggc
     6901 acaggaggca gccgcagcag tgatgcaggc tgctgcgaac tcagcacaaa gcaggcaagg
     6961 cagtttcccc ggcatgaacc agagtggact tatggcttcc agctctccct acagccagcc
     7021 catgaacaac agctctagcc tgatgaacac gcaggcgccg ccctacagca tggcgcccgc
     7081 catggtgaac agctcggcag catctgtggg tcttgcagat atgatgtctc ctggtgaatc
     7141 caaactgccc ctgcctctca aagcagacgg caaagaagaa ggcactccac agcccgagag
     7201 caagtcaaag aagtccagct cctccaccac tactggggag aagatcacga aggtgtacga
     7261 gctggggaat gagccagaga gaaagctctg ggtcgaccga tacctcacct tcatggaaga
     7321 gagaggctct cctgtctcaa gtctgcctgc cgtgggcaag aagcccctgg acctgttccg
     7381 actctacgtc tgcgtcaaag agatcggggg tttggcccag gttaataaaa acaagaagtg
     7441 gcgtgagctg gcaaccaacc taaacgttgg cacctcaagc agtgcagcga gctccctgaa
     7501 aaagcagtat attcagtacc tgtttgcctt tgagtgcaag atcgaacgtg gggaggagcc
     7561 cccgccggaa gtcttcagca ccggggacac caaaaagcag cccaagctcc agccgccatc
     7621 tcctgctaac tcgggatcct tgcaaggccc acagaccccc cagtcaactg gcagcaattc
     7681 catggcagag gttccaggtg acctgaagcc acctacccca gcctccaccc ctcacggcca
     7741 gatgactcca atgcaaggtg gaagaagcag tacaatcagt gtgcacgacc cattctcaga
     7801 tgtgagtgat tcatccttcc cgaaacggaa ctccatgact ccaaacgccc cctaccagca
     7861 gggcatgagc atgcccgatg tgatgggcag gatgccctat gagcccaaca aggacccctt
     7921 tgggggaatg agaaaagtgc ctggaagcag cgagcccttt atgacgcaag gacagatgcc
     7981 caacagcagc atgcaggaca tgtacaacca aagtccctcc ggagcaatgt ctaacctggg
     8041 catggggcag cgccagcagt ttccctatgg agccagttac gaccgaaggc atgaacctta
     8101 tgggcagcag tatccaggcc aaggccctcc ctcgggacag ccgccgtatg gagggcacca
     8161 gcccggcctg tacccacagc agccgaatta caaacgccat atggacggca tgtacgggcc
     8221 cccagccaag cgccacgagg gcgacatgta caacatgcag tacagcagcc agcagcagga
     8281 gatgtacaac cagtatggag gctcctactc gggcccggac cgcaggccca tccagggcca
     8341 gtacccgtat ccctacagca gggagaggat gcagggcccg gggcagatcc agacacacgg
     8401 aatcccgcct cagatgatgg gcggcccgct gcagtcgtcc tccagtgagg ggcctcagca
     8461 gaatatgtgg gcagcacgca atgatatgcc ttatccctac cagaacaggc agggccctgg
     8521 cggccctaca caggcgcccc cttacccagg catgaaccgc acagacgata tgatggtacc
     8581 cgatcagagg ataaatcatg agagccagtg gccttctcac gtcagccagc gtcagcctta
     8641 tatgtcgtcc tcagcctcca tgcagcccat cacacgccca ccacagccgt cctaccagac
     8701 gccaccgtca ctgccaaatc acatctccag ggcgcccagc ccagcgtcct tccagcgctc
     8761 cctggagaac cgcatgtctc caagcaagtc tccttttctg ccgtctatga agatgcagaa
     8821 ggtcatgccc acggtcccca catcccaggt caccgggcca ccaccccaac cacccccaat
     8881 cagaagggag atcacctttc ctcctggctc agtagaagca tcacaaccag tcttgaaaca
     8941 aaggcgaaag attacctcca aagatatcgt tactcctgag gcgtggcgtg tgatgatgtc
     9001 ccttaaatca ggtcttttgg ctgagagtac gtgggctttg gacactatta atattcttct
     9061 gtatgatgac agcactgttg ctactttcaa tctctcccag ttgtctggat ttctcgaact
     9121 tttagtcgag tactttagaa aatgcctgat tgacattttt ggaattctta tggaatatga
     9181 agtgggagac cccagccaaa aagcacttga tcacaacgca gcaaggaagg atgacagcca
     9241 gtccttggca gacgattctg ggaaagagga ggaagatgct gaatgtattg atgacgacga
     9301 ggaagacgag gaggatgagg aggaagacag cgagaagaca gaaagcgatg aaaagagcag
     9361 catcgctctg actgccccgg acgccgctgc agacccaaag gagaagccca agcaagccag
     9421 taagttcgac aagctgccaa taaagatagt caaaaagaac aacctgtttg ttgttgaccg
     9481 atctgacaag ttggggcgtg tgcaggagtt caatagtggc cttctgcact ggcagctcgg
     9541 cgggggtgac accaccgagc acattcagac tcactttgag agcaagatgg aaattcctcc
     9601 tcgcaggcgc ccacctcccc ccttaagctc cgcaggtaga aagaaagagc aagaaggcaa
     9661 aggcgactct gaagagcagc aagagaaaag catcatagca accatcgatg acgtcctctc
     9721 tgctcggcca ggggcattgc ctgaagacgc aaaccctggg ccccagaccg aaagcagtaa
     9781 gtttcccttt ggtatccagc aagccaaaag tcaccggaac atcaagctgc tggaggacga
     9841 gcccaggagc cgagacgaga ctcctctgtg taccatcgcg cactggcagg actcgctggc
     9901 taagcgatgc atctgtgtgt ccaatattgt ccgtagcttg tcattcgtgc ctggcaatga
     9961 tgccgaaatg tccaaacatc caggcctggt gctgatcctg gggaagctga ttcttcttca
    10021 ccacgagcat ccagagagaa agcgagcacc gcagacctat gagaaagagg aggatgagga
    10081 caagggggtg gcctgcagca aagatgagtg gtggtgggac tgcctcgagg tcttgaggga
    10141 taacacgttg gtcacgttgg ccaacatttc cgggcagcta gacttgtctg cttacacgga
    10201 aagcatctgc ttgccaattt tggatggctt gctgcactgg atggtgtgcc cgtctgcaga
    10261 ggcacaagat ccctttccaa ctgtgggacc caactcggtc ctgtcgcctc agagacttgt
    10321 gctggagacc ctctgtaaac tcagtatcca ggacaataat gtggacctga tcttggccac
    10381 tcctccattt agtcgtcagg agaaattcta tgctacatta gttaggtacg ttggggatcg
    10441 caaaaaccca gtctgtcgag aaatgtccat ggcgctttta tcgaaccttg cccaagggga
    10501 cgcactagca gcaagggcca tagctgtgca gaaaggaagc attggaaact tgataagctt
    10561 cctagaggat ggggtcacga tggcccagta ccagcagagc cagcacaacc tcatgcacat
    10621 gcagcccccg cccctggaac cacctagcgt agacatgatg tgcagggcgg ccaaggcttt
    10681 gctagccatg gccagagtgg acgaaaaccg ctcggaattc cttttgcacg agggccggtt
    10741 gctggatatc tcgatatcag ctgtcctgaa ctctctggtt gcatctgtca tctgtgatgt
    10801 actgtttcag attgggcagt tatagtaatg aactagtccg taacttgaaa gtatttcgat
    10861 ttcttggctt tatatatctt gtggaaagga cgatcgataa gcagaatccg ccgaatcacg
    10921 cgttaagtcg acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
    10981 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
    11041 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
    11101 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
    11161 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
    11221 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
    11281 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
    11341 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
    11401 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
    11461 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
    11521 cgtcgacttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
    11581 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt
    11641 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
    11701 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
    11761 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
    11821 ctagcagtac gtatagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
    11881 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa
    11941 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
    12001 tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc
    12061 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
    12121 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
    12181 aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac
    12241 gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
    12301 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
    12361 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc
    12421 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
    12481 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
    12541 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
    12601 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
    12661 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
    12721 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
    12781 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
    12841 aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta
    12901 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
    12961 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
    13021 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
    13081 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
    13141 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
    13201 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
    13261 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
    13321 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
    13381 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
    13441 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
    13501 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
    13561 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
    13621 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
    13681 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
    13741 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
    13801 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
    13861 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
    13921 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
    13981 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
    14041 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
    14101 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
    14161 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
    14221 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
    14281 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
    14341 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
    14401 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
    14461 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
    14521 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
    14581 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
    14641 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
    14701 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
    14761 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
    14821 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
    14881 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
    14941 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
    15001 gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa
    15061 agctggagct gcaagc
//
