LOCUS       sequence-401218-        2950 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Gilbert C, Brumwell SL, Crits-Christoph A, Kang SC, Martin-Moldes Z,
            Alsharif W, Esmurria A, Nguyen M-A, Lee HH, Ostrov N
  TITLE     A scalable framework for high-throughput identification of 
            functional origins of replication in non-model bacteria
  JOURNAL   bioRxiv 2023.05.19.541510
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.05.19.541510"
COMMENT     Sequence Label: pGL0_50 [catE]
FEATURES             Location/Qualifiers
     source          1..2950
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(994..1010)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(994..1010)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1007..1029)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(1182..1770)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1262..1281)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1903..1997)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2018..2677)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2592..2611
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2678..2780)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     2867..2889
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2881..2898
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2882..2898
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tagcgtgcga acagcacgct attgttgtat cattactcgc atccattctc aggctgtctc
       61 gtctcgtctc cgaggtctca aatgaacttt aataaaattg atttagacaa ttggaagaga
      121 aaagagatat ttaatcatta tttgaaccaa caaacgactt ttagtataac cacagaaatt
      181 gatattagtg ttttataccg aaacataaaa caagaaggat ataaatttta ccctgcattt
      241 attttcttag tgacaagggt gataaactca aatacagctt ttagaactgg ttacaatagc
      301 gacggagagt taggttattg ggataagtta gagccacttt atacaatttt tgatggtgta
      361 tctaaaacat tctctggtat ttggactcct gtaaagaatg acttcaaaga gttttatgat
      421 ttataccttt ctgatgtaga gaaatataat ggttcgggga aattgtttcc caaaacacct
      481 atacctgaaa atactttttc tctttctatt attccatgga cttcatttac tgggtttaac
      541 ttaaatatca ataataatag taattacctt ctacccatta ttacagcagg aaaattcatt
      601 aataaaggta attcaatata tttaccgcta tctttacagg tacatcattc tgtttgtgat
      661 ggttatcatg caggattgtt tatgaactct attcaggaat tgtcagatag gcctaatgac
      721 tggcttttat aagctttgag accaccccag gatacataga ttaccacaac tccgagccct
      781 tccacctagc gtgctaacca cgcacgctat tgttgtatcc catataatgc aatcgtagtc
      841 tacctgactg tacttagaaa tgtggcttcg cctttgccca cgcacctgat cgctcctcgt
      901 ttgcttttaa ggaccggacg aaccacagag cattagaagg ctaggtggag gctcagtgat
      961 gataagtctg cgatggtgga tgcatgtgtc atggtcatag ctgtttcctg tgtgaaattg
     1021 ttatccgctc agagggcaca atcctattcc gcgctatccg acaatctcca agacattagg
     1081 tggagttcag ttcggcgtat ggcatatgtc gctggaaaga acatgtgagc aaaaggccag
     1141 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     1201 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     1261 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     1321 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     1381 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     1441 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     1501 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     1561 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     1621 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     1681 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     1741 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     1801 gacgctctat tcaacaaagc cgccgtcccg tcaagtcagc gtaaatgggt agggggcttc
     1861 aaatcgtccc cccatacgat ataagttgtt actagtgctt ggattctcac caataaaaaa
     1921 cgcccggcgg caaccgagcg ttctgaacaa atccagatgg agttctgagg tcattactgg
     1981 atctatcaac aggagtccaa gcgagctcga tatcaaatta cgccccgccc tgccactcat
     2041 cgcagtactg ttgtaattca ttaagcattc tgccgacatg gaagccatca caaacggcat
     2101 gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca
     2161 tggtgaaaac gggggcgaag aagttgtcca tattggccac gtttaaatca aaactggtga
     2221 aactcaccca gggattggct gagacgaaaa acatattctc aataaaccct ttagggaaat
     2281 aggccaggtt ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga aactgccgga
     2341 aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc agtttgctca tggaaaacgg
     2401 tgtaacaagg gtgaacacta tcccatatca ccagctcacc gtctttcatt gccatacgaa
     2461 attccggatg agcattcatc aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt
     2521 gcttattttt ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg gtctggttat
     2581 aggtacattg agcaactgac tgaaatgcct caaaatgttc tttacgatgc cattgggata
     2641 tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa
     2701 atctcgataa ctcaaaaaat acgcccggta gtgatcttat ttcattatgg tgaaagttgg
     2761 aacctcttac gtgccgatca acgtctcatt gatacctgaa acaaaaccca tcgtacggcc
     2821 aaggaagtct ccaataactg tgatccacca caagcgccag ggttttccca gtcacgacgt
     2881 tgtaaaacga cggccagtca tgcataatcc gcacgcatct ggaataagga agtgccattc
     2941 cgcctgacct
//
