LOCUS       sequence-401225-        2231 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Gilbert C, Brumwell SL, Crits-Christoph A, Kang SC, Martin-Moldes Z,
            Alsharif W, Esmurria A, Nguyen M-A, Lee HH, Ostrov N
  TITLE     A scalable framework for high-throughput identification of 
            functional origins of replication in non-model bacteria
  JOURNAL   bioRxiv 2023.05.19.541510
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.05.19.541510"
COMMENT     Sequence Label: pGL0_113 [Dummy ORI]
FEATURES             Location/Qualifiers
     source          1..2231
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(244..338)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(359..1018)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     933..952
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(1019..1121)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     1208..1230
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1222..1239
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1223..1239
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1566..1582)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1566..1582)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1579..1601)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(join(1754..2231,1..111))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1834..1853)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
       61 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
      121 ttgatctttt ctacggggtc tgacgctcta ttcaacaaag ccgccgtccc gtcaagtcag
      181 cgtaaatggg tagggggctt caaatcgtcc ccccatacga tataagttgt tactagtgct
      241 tggattctca ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca aatccagatg
      301 gagttctgag gtcattactg gatctatcaa caggagtcca agcgagctcg atatcaaatt
      361 acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat
      421 ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc
      481 cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc atattggcca
      541 cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa aacatattct
      601 caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat
      661 atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt
      721 cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc accagctcac
      781 cgtctttcat tgccatacga aattccggat gagcattcat caggcgggca agaatgtgaa
      841 taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag gccgtaatat
      901 ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt
      961 ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt
     1021 tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt agtgatctta
     1081 tttcattatg gtgaaagttg gaacctctta cgtgccgatc aacgtctcat tgatacctga
     1141 aacaaaaccc atcgtacggc caaggaagtc tccaataact gtgatccacc acaagcgcca
     1201 gggttttccc agtcacgacg ttgtaaaacg acggccagtc atgcataatc cgcacgcatc
     1261 tggaataagg aagtgccatt ccgcctgacc ttagcgtgcg aacagcacgc tattgttgta
     1321 tcattactcg catccattct caggctgtct cgtctcgtct ccgaggtctc cggagattcc
     1381 atgcgcggac cataccgtca attcttcggt tatgtttccg atgcgctcga gaccacccca
     1441 ggatacatag attaccacaa ctccgagccc ttccacctag cgtgctaacc acgcacgcta
     1501 ttgttgtata ggctaggtgg aggctcagtg atgataagtc tgcgatggtg gatgcatgtg
     1561 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcagagggca caatcctatt
     1621 ccgcgctatc cgacaatctc caagacatta ggtggagttc agttcggcgt atggcatatg
     1681 tcgctggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     1741 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     1801 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     1861 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     1921 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     1981 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     2041 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     2101 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     2161 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     2221 tgaagccagt t
//
