LOCUS       sequence-401242-        2566 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Gilbert C, Brumwell SL, Crits-Christoph A, Kang SC, Martin-Moldes Z,
            Alsharif W, Esmurria A, Nguyen M-A, Lee HH, Ostrov N
  TITLE     A scalable framework for high-throughput identification of 
            functional origins of replication in non-model bacteria
  JOURNAL   bioRxiv 2023.05.19.541510
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.05.19.541510"
COMMENT     Sequence Label: pGL0_21 [UARS1 ORI]
FEATURES             Location/Qualifiers
     source          1..2566
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(439..533)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(554..1213)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1128..1147
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(1214..1316)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     1403..1425
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1417..1434
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1418..1434
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2096..2112)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2096..2112)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2109..2131)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(join(2284..2566,1..306))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2364..2383)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
       61 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
      121 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
      181 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
      241 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
      301 tctcaagaag atcctttgat cttttctacg gggtctgacg ctctattcaa caaagccgcc
      361 gtcccgtcaa gtcagcgtaa atgggtaggg ggcttcaaat cgtcccccca tacgatataa
      421 gttgttacta gtgcttggat tctcaccaat aaaaaacgcc cggcggcaac cgagcgttct
      481 gaacaaatcc agatggagtt ctgaggtcat tactggatct atcaacagga gtccaagcga
      541 gctcgatatc aaattacgcc ccgccctgcc actcatcgca gtactgttgt aattcattaa
      601 gcattctgcc gacatggaag ccatcacaaa cggcatgatg aacctgaatc gccagcggca
      661 tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg gcgaagaagt
      721 tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaga
      781 cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg
      841 ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga
      901 gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc
      961 atatcaccag ctcaccgtct ttcattgcca tacgaaattc cggatgagca ttcatcaggc
     1021 gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta
     1081 aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa
     1141 atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta tatccagtga
     1201 tttttttctc cattttagct tccttagctc ctgaaaatct cgataactca aaaaatacgc
     1261 ccggtagtga tcttatttca ttatggtgaa agttggaacc tcttacgtgc cgatcaacgt
     1321 ctcattgata cctgaaacaa aacccatcgt acggccaagg aagtctccaa taactgtgat
     1381 ccaccacaag cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtcatgca
     1441 taatccgcac gcatctggaa taaggaagtg ccattccgcc tgaccttagc gtgcgaacag
     1501 cacgctattg ttgtatcatt actcgcatcc attctcaggc tgtctcgtct cgtctccgag
     1561 gtctccggag attaccgatc ctcgatcttt gtgcaagcta gcccgcctcg gcagcaacaa
     1621 agcagccgag caagaagcag tacttgcctt ctgaatcgtg aatgggttac gttcttcacc
     1681 gctgtgatca gcgaatcatg aatcaaatca tgagggcatt gctgatcatg aatcaaatca
     1741 tgagggcatt taaaaattca gtctgagtcg tgagtagcaa gtcggttctg gatcggatgg
     1801 cattcatgaa tcacagggtc gtgaatcatg aatgttcaag tccccttttc tcgagaggct
     1861 ggtgggatcg gtgcgaatca cgaatcatga ttgtaattca ttgagtgaag gagtttcgca
     1921 gccacccaca gtactagaat cacgaatgac aatcgctcga gaccacccca ggatacatag
     1981 attaccacaa ctccgagccc ttccacctag cgtgctaacc acgcacgcta ttgttgtata
     2041 ggctaggtgg aggctcagtg atgataagtc tgcgatggtg gatgcatgtg tcatggtcat
     2101 agctgtttcc tgtgtgaaat tgttatccgc tcagagggca caatcctatt ccgcgctatc
     2161 cgacaatctc caagacatta ggtggagttc agttcggcgt atggcatatg tcgctggaaa
     2221 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     2281 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     2341 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     2401 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     2461 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     2521 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgc
//
