LOCUS       sequence-401263-        2547 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Gilbert C, Brumwell SL, Crits-Christoph A, Kang SC, Martin-Moldes Z,
            Alsharif W, Esmurria A, Nguyen M-A, Lee HH, Ostrov N
  TITLE     A scalable framework for high-throughput identification of 
            functional origins of replication in non-model bacteria
  JOURNAL   bioRxiv 2023.05.19.541510
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.05.19.541510"
COMMENT     Sequence Label: pGL0_105 [tAnTrpC]
FEATURES             Location/Qualifiers
     source          1..2547
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(591..607)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(591..607)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(604..626)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(779..1367)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(859..878)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1500..1594)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1615..2274)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2189..2208
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2275..2377)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     2464..2486
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2478..2495
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2479..2495
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tagcgtgcga acagcacgct attgttgtat cattactcgc atccattctc aggctgtctc
       61 gtctcgtctc cgaggtctca gcttacttaa cgttactgaa atcatcaaac agcttgacga
      121 atctggatat aagatcgttg gtgtcgatgt cagctccgga gttgagacaa atggtgttca
      181 ggatctcgat aagatacgtt catttgtcca agcagcaaag agtgccttct agtgatttaa
      241 tagctccatg tcaacaagaa taaaacgcgt ttcgggttta cctcttccag atacagctca
      301 tctgcaatgc atcgaccgtc tgagacatgc gcttgagacc accccaggat acatagatta
      361 ccacaactcc gagcccttcc acctagcgtg ctaaccacgc acgctattgt tgtatcccat
      421 ataatgcaat cgtagtctac ctgactgtac ttagaaatgt ggcttcgcct ttgcccacgc
      481 acctgatcgc tcctcgtttg cttttaagga ccggacgaac cacagagcat tagaaggcta
      541 ggtggaggct cagtgatgat aagtctgcga tggtggatgc atgtgtcatg gtcatagctg
      601 tttcctgtgt gaaattgtta tccgctcaga gggcacaatc ctattccgcg ctatccgaca
      661 atctccaaga cattaggtgg agttcagttc ggcgtatggc atatgtcgct ggaaagaaca
      721 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
      781 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
      841 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
      901 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
      961 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     1021 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     1081 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     1141 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     1201 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     1261 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     1321 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     1381 tcttttctac ggggtctgac gctctattca acaaagccgc cgtcccgtca agtcagcgta
     1441 aatgggtagg gggcttcaaa tcgtcccccc atacgatata agttgttact agtgcttgga
     1501 ttctcaccaa taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt
     1561 tctgaggtca ttactggatc tatcaacagg agtccaagcg agctcgatat caaattacgc
     1621 cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa
     1681 gccatcacaa acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg
     1741 cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt
     1801 taaatcaaaa ctggtgaaac tcacccaggg attggctgag acgaaaaaca tattctcaat
     1861 aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat
     1921 gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt
     1981 ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc
     2041 tttcattgcc atacgaaatt ccggatgagc attcatcagg cgggcaagaa tgtgaataaa
     2101 ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag
     2161 ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt
     2221 acgatgccat tgggatatat caacggtggt atatccagtg atttttttct ccattttagc
     2281 ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc
     2341 attatggtga aagttggaac ctcttacgtg ccgatcaacg tctcattgat acctgaaaca
     2401 aaacccatcg tacggccaag gaagtctcca ataactgtga tccaccacaa gcgccagggt
     2461 tttcccagtc acgacgttgt aaaacgacgg ccagtcatgc ataatccgca cgcatctgga
     2521 ataaggaagt gccattccgc ctgacct
//
