LOCUS       sequence-401724-        1107 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression in Schneider cells for imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Salgania HK, Metz J, Jeske M
  TITLE     ReLo is a simple and rapid colocalization assay to identify and 
            characterize direct protein-protein interactions.
  JOURNAL   Nat Commun. 2024 Apr 3;15(1):2875. doi: 10.1038/s41467-024-47233-4.
  PUBMED    38570497
REFERENCE   2  (bases 1 to 1107)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-04-3"; volume: "15(1):2875. doi"; pages: " 
            10.1038/s41467-024-47233-4"
COMMENT     Sequence Label: pAc5.1-PH-mCherry (FspAI) (HK49)
FEATURES             Location/Qualifiers
     source          1..1107
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             400..1107
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(545..563)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     813..832
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 atgcacggtc tccaggatga tccagatctg caggctctgc tgaaaggttc tcagctcctc
       61 aaagtcaaaa gcagctcctg gcgtcgtgaa cgcttctaca aactgcagga ggactgcaaa
      121 accatctggc aggaatcccg taaagtaatg cgctccccag agtctcagct cttcagcatt
      181 gaggacattc aggaggtacg tatgggtcac cgtaccgaag gtctggagaa attcgctcgc
      241 gatatcccag aagatcgctg cttctctatc gtcttcaaag accagcgcaa caccctggat
      301 ctgattgctc catctccagc agatgctcag cactgggttc agggtctccg caaaatcatt
      361 caccactccg gttctatgga ccagcgtcag aaaggtacca tggtgagcaa gggcgaggag
      421 gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg
      481 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag
      541 accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc
      601 cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac
      661 ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc
      721 ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg
      781 aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc
      841 tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag
      901 cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag
      961 gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc
     1021 tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc
     1081 accggcggca tggacgagct gtacaag
//
