LOCUS       sequence-401805-       10474 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of Flag-Tagged human LARP7 and the snRNA 7SK.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10474)
  TITLE     Alarcon lab plasmids
REFERENCE   2  (bases 1 to 10474)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLKO.1 LARP7-FLAG m6A mut 7SK
FEATURES             Location/Qualifiers
     source          1..10474
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     154..171
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    288..309
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(324..329,330..347,348..354)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    362..378
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     367..389
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     386..402
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     386..402
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     421..441
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        423..441
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        469..695
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             696..876
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    923..1048
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1541..1774
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1959..2003
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2301..2549
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2301..2321
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2472..2491
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    3136..3253
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3302..3812
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3731..3749
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             5615..5644
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5663..5686
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    5720..6293
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5887..5904)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6114..6133
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6313..7338
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             6313..7338
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     complement(7362..7378)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7363..7379)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7461..7694
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7766..7887
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7803..7822)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7857..7876
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7927..8062
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8082..8101)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8083..8101)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8111..8128)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8111..8127)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8120..8142)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8269..8724
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8356..8375)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8566..8587
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8750..8854
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8855..8923,8924..9715)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9073..9092)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9886..10474
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10375..10394
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
       61 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      121 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      181 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
      241 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
      301 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
      361 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc
      421 gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg
      481 caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag
      541 agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag
      601 gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag
      661 atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct
      721 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
      781 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
      841 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
      901 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
      961 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
     1021 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
     1081 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
     1141 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
     1201 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
     1261 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
     1321 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
     1381 gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
     1441 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
     1501 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
     1561 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
     1621 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
     1681 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
     1741 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
     1801 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
     1861 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
     1921 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
     1981 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
     2041 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
     2101 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
     2161 caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca
     2221 gagacagatc cattcgatta gtgaacggat ctcgacggta tcgatcacga gactagcctc
     2281 gagcggccgc ccccttcacc gagggcctat ttcccatgat tccttcatat ttgcatatac
     2341 gatacaaggc tgttagagag ataattggaa ttaatttgac tgtaaacaca aagatattag
     2401 tacaaaatac gtgacgtaga aagtaataat ttcttgggta gtttgcagtt ttaaaattat
     2461 gttttaaaat ggactatcat atgcttaccg taacttgaaa gtatttcgat ttcttggctt
     2521 tatatatctt gtggaaagga cgaaacaccg ggatgtgagg gcgatctggc tgcgacatct
     2581 gtcaccccat tgatcgccag ggttgattcg gctgatctgg ctggctaggc gggtgtcccc
     2641 ttcctccctc accgctccat gtgcgtccct cccgaagctg cgcgctcggt cgaagaggtc
     2701 gaccatcccc gatagaggag ggccggtctt cggtcaaggg tatacgagta gctgcgctcc
     2761 cctgctagat cctccaagca agctctcaag gtccatttgt aggagatcgt agggtagtca
     2821 agcttccaag gctccaggca catccaaatg aggcgctgca tgtggcagtc tgcctttctt
     2881 ttgacccatt acccatctaa gttagatgct tttttaaatg ttttttaatt tttaaatttt
     2941 taattttttt cattatttat tttttatttt tgagacggat ctcggctcac tgtaacctcc
     3001 acctcccggg ttcaagcgat tctcctgact cagcctcccg agtactggga ttacaggcgc
     3061 gcgccaccat gccggtccgc aggtatgcac gcgtgaattc tcgacctcga gacaaatggc
     3121 agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag
     3181 aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa
     3241 aattcaaaat tttcgggttt attacaggga cagcagagat ccactttggc cgcggctcga
     3301 gggggttggg gttgcgcctt ttccaaggca gccctgggtt tgcgcaggga cgcggctgct
     3361 ctgggcgtgg ttccgggaaa cgcagcggcg ccgaccctgg gtctcgcaca ttcttcacgt
     3421 ccgttcgcag cgtcacccgg atcttcgccg ctacccttgt gggccccccg gcgacgcttc
     3481 ctgctccgcc cctaagtcgg gaaggttcct tgcggttcgc ggcgtgccgg acgtgacaaa
     3541 cggaagccgc acgtctcact agtaccctcg cagacggaca gcgccaggga gcaatggcag
     3601 cgcgccgacc gcgatgggct gtggccaata gcggctgctc agcagggcgc gccgagagca
     3661 gcggccggga aggggcggtg cgggaggcgg ggtgtggggc ggtagtgtgg gccctgttcc
     3721 tgcccgcgcg gtgttccgca ttctgcaagc ctccggagcg cacgtcggca gtcggctccc
     3781 tcgttgaccg aatcaccgac ctctctcccc agggggatcc ggtaccgagg agatctgccg
     3841 ccgcgatcgc atggaaactg aaagtggaaa tcaggaaaag gtaatggaag aagaaagcac
     3901 tgaaaagaaa aaagaagttg aaaaaaagaa acggtcacga gttaaacagg tgcttgcaga
     3961 tattgctaag caagtggact tctggtttgg ggatgcaaat cttcacaagg atagatttct
     4021 tcgagaacag atagaaaaat ctagagatgg atatgttgat atatcactac ttgtgtcttt
     4081 taacaaaatg aaaaaattga ctactgatgg gaagttaatt gccagagcgt tgagaagttc
     4141 agctgttgta gagcttgatt tggaaggcac cagaatccgg aggaaaaaac ctctggggga
     4201 aagaccaaag gatgaggatg aacgcacagt gtatgtggag ttacttccca aaaatgttaa
     4261 tcacagctgg attgaaagag tatttgggaa atgtggcaat gttgtttata taagtatacc
     4321 acattataag tctactggag atccaaaggg atttgcgttt gtggaatttg aaacaaaaga
     4381 acaagcagca aaagcaattg agtttcttaa caacccacca gaagaagcac caagaaaacc
     4441 tggcatattt cctaaaacag tgaaaaataa gcccattcca gccttaagag ttgtggaaga
     4501 gaagaaaaag aaaaagaaga agaaaggccg aatgaaaaag gaagacaata tccaagccaa
     4561 agaagaaaac atggacacaa gcaacaccag catcagtaaa atgaaaagat ccagacccac
     4621 atctgagggc tctgacattg agtccactga accccaaaag cagtgctcaa agaaaaagaa
     4681 aaaacgggac agagttgaag catctagctt acctgaagtc agaacaggga agaggaagag
     4741 aagcagctct gaagatgcag aatccctagc tccccgatca aaagtaaaga aaattattca
     4801 gaaagacatc attaaggaag catcagaagc ttccaaggaa aatagagata tagaaatctc
     4861 tactgaagag gaaaaggata ctggagatct aaaagatagc tctctcttga aaacaaaaag
     4921 gaaacataag aaaaaacata aagagagaca taaaatggga gaagaagtta taccattaag
     4981 agtgctatca aagagcgaat ggatggattt gaaaaaagag tatttagcgc tacaaaaagc
     5041 tagcatggct tctttaaaaa aaacaatatc ccaaataaaa tcagagtcag aaatggaaac
     5101 agacagtgga gtacctcaaa acactggaat gaaaaatgaa aaaacagcca acagggaaga
     5161 gtgtcgcacc caggagaaag ttaatgcaac aggaccacag ttcgtgagtg gagtgattgt
     5221 gaagatcatt agcacagagc ctctacctgg caggaaacaa gtccgggata ctttggcagc
     5281 aatctcagaa gttctttatg ttgatttgct agaaggggat acagaatgcc atgctagatt
     5341 taaaactcct gaggatgctc aagcagtaat aaatgcctat acagaaatta acaagaaaca
     5401 ctgctggaaa ctcgagatcc tttctggtga tcacgaacaa aggtattggc agaagatttt
     5461 ggttgataga caggcaaaac ttaatcagcc tcgggaaaag aaaagaggca ctgaaaagtt
     5521 aatcaccaaa gctgaaaaga ttaggctggc aaagactcaa caagcgagta aacatataag
     5581 attttctgaa tatgatacgc gtacgcggcc gctcgagcag aaactcatct cagaagagga
     5641 tctggcagca aatgatatcc tggattacaa ggatgacgac gataaggttt aaacctaggc
     5701 gtagcggccg caaattccgc ccctctccct cccccccccc taacgttact ggccgaagcc
     5761 gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt
     5821 ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc
     5881 tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc
     5941 tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc
     6001 cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg
     6061 cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct
     6121 cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat
     6181 ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc
     6241 taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga taatcccggg
     6301 gggcaatgag atatgaaaaa gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc
     6361 gaaaagttcg acagcgtctc cgacctgatg cagctctcgg agggcgaaga atctcgtgct
     6421 ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg taaatagctg cgccgatggt
     6481 ttctacaaag atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc gattccggaa
     6541 gtgcttgaca ttggggaatt tagcgagagc ctgacctatt gcatctcccg ccgtgcacag
     6601 ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg ctgttctgca gccggtcgcg
     6661 gaggccatgg atgcgatcgc tgcggccgat cttagccaga cgagcgggtt cggcccattc
     6721 ggaccgcaag gaatcggtca atacactaca tggcgtgatt tcatatgcgc gattgctgat
     6781 ccccatgtgt atcactggca aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag
     6841 gctctcgatg agctgatgct ttgggccgag gactgccccg aagtccggca cctcgtgcac
     6901 gcggatttcg gctccaacaa tgtcctgacg gacaatggcc gcataacagc ggtcattgac
     6961 tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt cttctggagg
     7021 ccgtggttgg cttgtatgga gcagcagacg cgctacttcg agcggaggca tccggagctt
     7081 gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg gtcttgacca actctatcag
     7141 agcttggttg acggcaattt cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc
     7201 gtccgatccg gagccgggac tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc
     7261 tggaccgatg gctgtgtaga agtactcgcc gatagtggaa accgacgccc cagcactcgt
     7321 ccgagggcaa aggaatagag tagatgccga ccgggatcta tcgataccgt cgacctcgag
     7381 ggggggcccg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     7441 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct
     7501 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     7561 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     7621 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     7681 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
     7741 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata
     7801 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     7861 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact
     7921 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     7981 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     8041 tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt gagtcgtatt
     8101 acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     8161 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     8221 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta
     8281 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     8341 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     8401 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     8461 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     8521 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     8581 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     8641 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     8701 acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     8761 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     8821 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     8881 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     8941 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     9001 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     9061 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     9121 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     9181 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     9241 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     9301 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     9361 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     9421 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     9481 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     9541 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     9601 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     9661 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     9721 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     9781 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     9841 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     9901 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     9961 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
    10021 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
    10081 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
    10141 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
    10201 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
    10261 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
    10321 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
    10381 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
    10441 gtgatgctcg tcaggggggc ggagcctatg gaaa
//
