LOCUS       sequence-402531-        9233 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MCL1 overexpression vector with G418 resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9233)
  AUTHORS   Girish V, Lakhani AA, Thompson SL, Scaduto CM, Brown LM, Hagenson 
            RA, Sausville EL, Mendelson BE, Kandikuppa PK, Lukow DA, Yuan ML, 
            Stevens EC, Lee SN, Schukken KM, Akalu SM, Vasudevan A, Zou C, 
            Salovska B, Li W, Smith JC, Taylor AM, Martienssen RA, Liu Y, Sun R,
            Sheltzer JM
  TITLE     Oncogene-like addiction to aneuploidy in human cancers.
  JOURNAL   Science. 2023 Jul 6:eadg4521. doi: 10.1126/science.adg4521.
  PUBMED    37410869
REFERENCE   2  (bases 1 to 9233)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2023 Jul 6:eadg4521. doi: 10.1126/science.adg4521."
COMMENT     Sequence Label: pLVX-Neo-CMV-MCL1
FEATURES             Location/Qualifiers
     source          1..9233
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(392..416)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    491..608
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        657..1167
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     1086..1104
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             1189..1992
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1252..1271)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1862..1881
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             2131..2364
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2436..2557
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2473..2492)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2527..2546
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2597..2732
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2752..2771)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2753..2771)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2781..2798)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2781..2797)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2790..2812)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2939..3394
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3026..3045)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3236..3257
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3420..3524
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3525..3593,3594..4385)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3743..3762)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4556..5144
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5045..5064
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5298..5315
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5432..5453
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5468..5473,5474..5491,5492..5498)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5506..5522
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5511..5533
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5530..5546
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5530..5546
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5565..5585
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5567..5585
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5613..5839
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             5840..6020
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6067..6192
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6685..6918
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7103..7147
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7445..7693
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     7445..7465
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     7616..7635
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     enhancer        7848..8227
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8228..8431
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8382..8402
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     8428..8452
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    8510..8534
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tccaaggcat gcttcggaaa ctggacatca aaaacgaaga cgatgtgaaa tcgttgtctc
       61 gagtgatgat ccatgttttc agcgacggcg taacaaactg gggcaggatt gtgactctca
      121 tttcttttgg tgcctttgtg gctaaacact tgaagaccat aaaccaagaa agctgcatcg
      181 aaccattagc agaaagtatc acagacgttc tcgtaaggac aaaacgggac tggctagtta
      241 aacaaagagg ctgggatggg tttgtggagt tcttccatgt agaggaccta gaaggtggca
      301 tcaggaatgt gctgctggct tttgcaggtg ttgctggagt aggagctggt ttggcatatc
      361 taataagata gaagagcccg ggcgatcgcg aacccagctt tcttgtacaa agtggttgat
      421 cgcgtgcatg cgacgtcata gctctctccc aattctcgac ctcgagacaa atggcagtat
      481 tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
      541 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
      601 aaaattttcg ggtttattac agggacagca gagatccact ttggccgcgg ctcgaggggg
      661 ttggggttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg
      721 cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt
      781 cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct
      841 ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa
      901 gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc
      961 cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc
     1021 cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc
     1081 gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt
     1141 gaccgaatca ccgacctctc tccccagggg gatccaccgg agcttaccat gggatcggcc
     1201 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
     1261 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
     1321 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag
     1381 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
     1441 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
     1501 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
     1561 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
     1621 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
     1681 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc
     1741 gatgatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
     1801 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
     1861 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
     1921 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
     1981 gagttcttct gacgcccgcc ccacgacccg cagcgcccga ccgaaaggag cgcacgaccc
     2041 catgcatcgt taagagctcg gtacctttaa gaccaatgac ttacaaggca gctgtagatc
     2101 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     2161 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     2221 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     2281 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     2341 agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat
     2401 aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg
     2461 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     2521 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc
     2581 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     2641 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     2701 ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt
     2761 gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     2821 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     2881 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac
     2941 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     3001 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     3061 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     3121 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     3181 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     3241 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     3301 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     3361 gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc
     3421 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     3481 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     3541 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     3601 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     3661 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     3721 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     3781 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     3841 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     3901 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     3961 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     4021 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     4081 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     4141 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     4201 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     4261 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     4321 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     4381 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     4441 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     4501 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     4561 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     4621 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     4681 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     4741 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     4801 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     4861 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     4921 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     4981 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     5041 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     5101 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     5161 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     5221 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     5281 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     5341 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     5401 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     5461 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     5521 aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact
     5581 aaagggaaca aaagctggag ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc
     5641 ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc
     5701 atgccgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc aacagacggg
     5761 tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg tatttaagtg
     5821 cctagctcga tacataaacg ggtctctctg gttagaccag atctgagcct gggagctctc
     5881 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     5941 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     6001 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
     6061 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
     6121 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
     6181 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
     6241 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
     6301 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg
     6361 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
     6421 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
     6481 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat
     6541 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
     6601 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
     6661 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
     6721 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
     6781 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
     6841 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
     6901 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
     6961 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
     7021 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
     7081 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
     7141 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
     7201 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag
     7261 agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc
     7321 cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg
     7381 attagtgaac ggatctcgac ggtatcgatc acgagactag cctcgagcgg ccgccccctt
     7441 caccgagggc ctatttccca tgattccttc atatttgcat atacgataca aggctgttag
     7501 agagataatt ggaattaatt tgactgtaaa cacaaagata ttagtacaaa atacgtgacg
     7561 tagaaagtaa taatttcttg ggtagtttgc agttttaaaa ttatgtttta aaatggacta
     7621 tcatatgctt accgtaactt gaaagtattt cgatttcttg gctttatata tcttgtggaa
     7681 aggacgaaac accggtatct atacattgaa tcaatattgg caattagcca tattagtcat
     7741 tggttatata gcataaatca atattggcta ttggccattg catacgttgt atctatatca
     7801 taatatgtac atttatattg gctcatgtcc aatatgaccg ccatgttgac attgattatt
     7861 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
     7921 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
     7981 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
     8041 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
     8101 gccaagtccg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
     8161 gtacatgacc ttacgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
     8221 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
     8281 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
     8341 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
     8401 tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga tcgcctggag
     8461 acgccatcca cgctgttttg acctccatag aagattctag attcgaagaa caagtttgta
     8521 caaaaaagca ggcttgaagg aattcggtac catgtttggc ctcaaaagaa acgcggtaat
     8581 cggactcaac ctctactgtg ggggggccgg cttgggggcc ggcagcggcg gcgccacccg
     8641 cccgggaggg cgacttttgg ctacggagaa ggaggcctcg gcccggcgag agataggggg
     8701 aggggaggcc ggcgcggtga ttggcggaag cgccggcgca agccccccgt ccaccctcac
     8761 gccagactcc cggagggtcg cgcggccgcc gcccattggc gccgaggtcc ccgacgtcac
     8821 cgcgaccccc gcgaggctgc ttttcttcgc gcccacccgc cgcgcggcgc cgcttgagga
     8881 gatggaagcc ccggccgctg acgccatcat gtcgcccgaa gaggagctgg acgggtacga
     8941 gccggagcct ctcgggaagc ggccggctgt cctgccgctg ctggagttgg tcggggaatc
     9001 tggtaataac accagtacgg acgggtcact accctcgacg ccgccgccag cagaggagga
     9061 ggaggacgag ttgtaccggc agtcgctgga gattatctct cggtaccttc gggagcaggc
     9121 caccggcgcc aaggacacaa agccaatggg caggtctggg gccaccagca ggaaggcgct
     9181 ggagacctta cgacgggttg gggatggcgt gcagcgcaac cacgagacgg cct
//
