LOCUS       sequence-402570-       12351 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus transfer vector for expression of huntingtin protein in 
            insect and mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12351)
  TITLE     Unpublished huntingtin plasmids
REFERENCE   2  (bases 1 to 12351)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: huntingtin_T97-M2069_NbioCflag
FEATURES             Location/Qualifiers
     source          1..12351
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      102..556
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(189..208)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     398..419
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        582..686
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(687..755,756..1547)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(905..924)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1718..2306
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2207..2226
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2460..2477
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  2611..2835
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2902..3435)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3624..3629,3630..3646,3647..3652))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     polyA_signal    4160..4208
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     4233..4252
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     enhancer        4313..4692
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4693..4896
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4847..4867
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        4943..5052
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     CDS             5087..5131
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             11099..11122
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             11144..11173
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             11174..11197
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(11287..11307)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(11705..11716)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    11941..12075
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11978..11997)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12032..12051
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  12104..12269
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga
       61 ctgaatatca acgcttattt gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
      121 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
      181 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
      241 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
      301 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
      361 ttttcgccct ttgacgttgg agtccacgtt cttaatagtg gactcttgtt ccaaactgga
      421 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
      481 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
      541 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
      601 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
      661 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
      721 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
      781 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
      841 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
      901 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
      961 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     1021 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     1081 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     1141 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     1201 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     1261 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     1321 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     1381 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     1441 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     1501 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     1561 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     1621 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     1681 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     1741 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     1801 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     1861 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     1921 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     1981 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     2041 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     2101 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     2161 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     2221 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     2281 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     2341 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     2401 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     2461 gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc
     2521 tgtgcggtat ttcacaccgc atagaccagc cgcgtaacct ggcaaaatcg gttacggttg
     2581 agtaataaat ggatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa
     2641 caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga
     2701 aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact
     2761 cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg
     2821 gtaaagacta tattcgcggc gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc
     2881 gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac
     2941 ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc
     3001 ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttcaaga gattgatgag
     3061 cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat
     3121 ctcactacgc ggctgctcaa acttgggcag aacgtaagcc gcgagagcgc caacaaccgc
     3181 ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta
     3241 atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag
     3301 atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat
     3361 gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt
     3421 gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg
     3481 cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga
     3541 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg
     3601 agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc
     3661 gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg
     3721 aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg
     3781 cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg cccttgcttc
     3841 aggagatcgg tagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag
     3901 tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag gactctagct
     3961 atagttctag tggttggcta cgtacccgta gtggctatgg cagggcttgc cgccccgacg
     4021 ttggctgcga gccctgggcc ttcacccgaa cttgggggtt ggggtgggga aaaggaagaa
     4081 acgcgggcgt attggtccca atggggtctc ggtggggtat cgacagagtg ccagccctgg
     4141 gaccgaaccc cgcgtttatg aacaaacgac ccaacacccg tgcgttttat tctgtctttt
     4201 tattgccgtc atagcgcggg ttccttccgg tattgtctcc ttccgtgttt cagttagcct
     4261 cccccatctc ccggtaccgc atgcggatct gtacgggcca gatatacgcg ttgacattga
     4321 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     4381 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     4441 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     4501 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     4561 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     4621 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     4681 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     4741 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     4801 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     4861 aggcgtgtac ggtgggaggt ctatataagc agagctctct ggctaactag agaacccact
     4921 gcttactggc ttatcgaata cggaccttta attcaaccca acacaatata ttatagttaa
     4981 ataagaatta ttatcaaatc atttgtatat taattaaaat actatactgt aaattacatt
     5041 ttatttacaa tcactcgacg gatccttaag aaggagatat actatgggac tcaatgacat
     5101 tttcgaagcc cagaagatag agtggcatga gggtggatct ggcggctcag ggggtagcgg
     5161 gggctccacc aagaaagacc gtgtgaatca ttgtctgaca atatgtgaaa acatagtggc
     5221 acagtctgtc agaaattctc cagaatttca gaaacttctg ggcatcgcta tggaactttt
     5281 tctgctgtgc agtgatgacg cagagtcaga tgtcaggatg gtggctgacg aatgcctcaa
     5341 caaagttatc aaagctttga tggattctaa tcttccaagg ttacagctcg agctctataa
     5401 ggaaattaaa aagaatggtg cccctcggag tttgcgtgct gccctgtgga ggtttgctga
     5461 gctggctcac ctggttcggc ctcagaaatg caggccttac ctggtgaacc ttctgccgtg
     5521 cctgactcga acaagcaaga gacccgaaga atcagtccag gagaccttgg ctgcagctgt
     5581 tcccaaaatt atggcttctt ttggcaattt tgcaaatgac aatgaaatta aggttttgtt
     5641 aaaggccttc atagcgaacc tgaagtcaag ctcccccacc attcggcgga cagcggctgg
     5701 atcagcagtg agcatctgcc agcactcaag aaggacacaa tatttctata gttggctact
     5761 aaatgtgctc ttaggcttac tcgttcctgt cgaggatgaa cactccactc tgctgattct
     5821 tggcgtgctg ctcaccctga ggtatttggt gcccttgctg cagcagcagg tcaaggacac
     5881 aagcctgaaa ggcagcttcg gagtgacaag gaaagaaatg gaagtctctc cttctgcaga
     5941 gcagcttgtc caggtttatg aactgacgtt acatcataca cagcaccaag accacaatgt
     6001 tgtgaccgga gccctggagc tgttgcagca gctcttcaga acgcctccac ccgagcttct
     6061 gcaaaccctg accgcagtcg ggggcattgg gcagctcacc gctgctaagg aggagtctgg
     6121 tggccgaagc cgtagtggga gtattgtgga acttatagct ggagggggtt cctcatgcag
     6181 ccctgtcctt tcaagaaaac aaaaaggcaa agtgctctta ggagaagaag aagccttgga
     6241 ggatgactct gaatcgagat cggatgtcag cagctctgcc ttaacagcct cagtgaagga
     6301 tgagatcagt ggagagctgg ctgcttcttc aggggtttcc actccagggt cagcaggtca
     6361 tgacatcatc acagaacagc cacggtcaca gcacacactg caggcggact cagtggatct
     6421 ggccagctgt gacttgacaa gctctgccac tgatggggat gaggaggata tcttgagcca
     6481 cagctccagc caggtcagcg ccgtcccatc tgaccctgcc atggacctga atgatgggac
     6541 ccaggcctcg tcgcccatca gcgacagctc ccagaccacc accgaagggc ctgattcagc
     6601 tgttacccct tcagacagtt ctgaaattgt gttagacggt accgacaacc agtatttggg
     6661 cctgcagatt ggacagcccc aggatgaaga tgaggaagcc acaggtattc ttcctgatga
     6721 agcctcggag gccttcagga actcttccat ggcccttcaa caggcacatt tattgaaaaa
     6781 catgagtcac tgcaggcagc cttctgacag cagtgttgat aaatttgtgt tgagagatga
     6841 agctactgaa ccgggtgatc aagaaaacaa gccttgccgc atcaaaggtg acattggaca
     6901 gtccactgat gatgactctg cacctcttgt ccattgtgtc cgccttttat ctgcttcgtt
     6961 tttgctaaca gggggaaaaa atgtgctggt tccggacagg gatgtgaggg tcagcgtgaa
     7021 ggccctggcc ctcagctgtg tgggagcagc tgtggccctc cacccggaat ctttcttcag
     7081 caaactctat aaagttcctc ttgacaccac ggaataccct gaggaacagt atgtctcaga
     7141 catcttgaac tacatcgatc atggagaccc acaggttcga ggagccactg ccattctctg
     7201 tgggaccctc atctgctcca tcctcagcag gtcccgcttc cacgtgggag attggatggg
     7261 caccattaga accctcacag gaaatacatt ttctttggcg gattgcattc ctttgctgcg
     7321 gaaaacactg aaggatgagt cttctgttac ttgcaagtta gcttgtacag ctgtgaggaa
     7381 ctgtgtcatg agtctctgca gcagcagcta cagtgagtta ggactgcagc tgatcatcga
     7441 tgtgctgact ctgaggaaca gttcctattg gctggtgagg acagagcttc tggaaaccct
     7501 tgcagagatt gacttcaggc tggtgagctt tttggaggca aaagcagaaa acttacacag
     7561 aggggctcat cattatacag ggcttttaaa actgcaagaa cgagtgctca ataatgttgt
     7621 catccatttg cttggagatg aagaccccag ggtgcgacat gttgccgcag catcactaat
     7681 taggcttgtc ccaaagctgt tttataaatg tgaccaagga caagctgatc cagtagtggc
     7741 cgtggcaaga gatcaaagca gtgtttacct gaaacttctc atgcatgaga cgcagcctcc
     7801 atctcatttc tccgtcagca caataaccag aatatataga ggctataacc tactaccaag
     7861 cataacagac gtcactatgg aaaataacct ttcaagagtt attgcagcag tttctcatga
     7921 actaatcaca tcaaccacca gagcactcac atttggatgc tgtgaagctt tgtgtcttct
     7981 ttccactgcc ttcccagttt gcatttggag tttaggttgg cactgtggag tgcctccact
     8041 gagtgcctca gatgagtcta ggaagagctg taccgttggg atggccacaa tgattctgac
     8101 cctgctctcg tcagcttggt tcccattgga tctctcagcc catcaagatg ctttgatttt
     8161 ggccggaaac ttgcttgcag ccagtgctcc caaatctctg agaagttcat gggcctctga
     8221 agaagaagcc aacccagcag ccaccaagca agaggaggtc tggccagccc tgggggaccg
     8281 ggccctggtg cccatggtgg agcagctctt ctctcacctg ctgaaggtga ttaacatttg
     8341 tgcccacgtc ctggatgacg tggctcctgg acccgcaata aaggcagcct tgccttctct
     8401 aacaaacccc ccttctctaa gtcccatccg acgaaagggg aaggagaaag aaccaggaga
     8461 acaagcatct gtaccgttga gtcccaagaa aggcagtgag gccagtgcag cttctagaca
     8521 atctgatacc tcaggtcctg ttacaacaag taaatcctca tcactgggga gtttctatca
     8581 tcttccttca tacctcaaac tgcatgatgt cctgaaagct acacacgcta actacaaggt
     8641 cacgctggat cttcagaaca gcacggaaaa gtttggaggg tttcttcgct cagccttgga
     8701 tgttctttct cagatactag agctggccac actgcaggac attgggaagt gtgttgaaga
     8761 gatcctagga tacctgaaat cctgctttag tcgagaacca atgatggcaa ctgtttgtgt
     8821 tcaacaattg ttgaagactc tctttggcac aaacttggcc tcccagtttg atggcttatc
     8881 ttccaacccc agcaagtcac aaggccgagc acagcgcctt ggctcctcca gtgtgaggcc
     8941 aggcttgtac cactactgct tcatggcccc gtacacccac ttcacccagg ccctcgctga
     9001 cgccagcctg aggaacatgg tgcaggcgga gcaggagaac gacacctcgg gatggtttga
     9061 tgtcctccag aaagtgtcta cccagttgaa gacaaacctc acgagtgtca caaagaaccg
     9121 tgcagataag aatgctattc ataatcacat tcgtttgttt gaacctcttg ttataaaagc
     9181 tttaaaacag tacacgacta caacatgtgt gcagttacag aagcaggttt tagatttgct
     9241 ggcgcagctg gttcagttac gggttaatta ctgtcttctg gattcagatc aggtgtttat
     9301 tggctttgta ttgaaacagt ttgaatacat tgaagtgggc cagttcaggg aatcagaggc
     9361 aatcattcca aacatctttt tcttcttggt attactatct tatgaacgct atcattcaaa
     9421 acagatcatt ggaattccta aaatcattca gctctgtgat ggcatcatgg ccagtggaag
     9481 gaaggctgtg acacatgcca taccggctct gcagcccata gtccacgacc tctttgtatt
     9541 aagaggaaca aataaagctg atgcaggaaa agagcttgaa acccaaaaag aggtggtggt
     9601 gtcaatgtta ctgagactca tccagtacca tcaggtgttg gagatgttca ttcttgtcct
     9661 gcagcagtgc cacaaggaga atgaagacaa gtggaagcga ctgtctcgac agatagctga
     9721 catcatcctc ccaatgttag ccaaacagca gatgcacatt gactctcatg aagcccttgg
     9781 agtgttaaat acattatttg agattttggc cccttcctcc ctccgtccgg tagacatgct
     9841 tttacggagt atgttcgtca ctccaaacac aatggcgtcc gtgagcactg ttcaactgtg
     9901 gatatcggga attctggcca ttttgagggt tctgatttcc cagtcaactg aagatattgt
     9961 tctttctcgt attcaggagc tctccttctc tccgtattta atctcctgta cagtaattaa
    10021 taggttaaga gatggggaca gtacttcaac gctagaagaa cacagtgaag ggaaacaaat
    10081 aaagaatttg ccagaagaaa cattttcaag gtttctatta caactggttg gtattctttt
    10141 agaagacatt gttacaaaac agctgaaggt ggaaatgagt gagcagcaac atactttcta
    10201 ttgccaggaa ctaggcacac tgctaatgtg tctgatccac atcttcaagt ctggaatgtt
    10261 ccggagaatc acagcagctg ccactaggct gttccgcagt gatggctgtg gcggcagttt
    10321 ctacaccctg gacagcttga acttgcgggc tcgttccatg atcaccaccc acccggccct
    10381 ggtgctgctc tggtgtcaga tactgctgct tgtcaaccac accgactacc gctggtgggc
    10441 agaagtgcag cagaccccga aaagacacag tctgtccagc acaaagttac ttagtcccca
    10501 gatgtctgga gaagaggagg attctgactt ggcagccaaa cttggaatgt gcaatagaga
    10561 aatagtacga agaggggctc tcattctctt ctgtgattat gtctgtcaga acctccatga
    10621 ctccgagcac ttaacgtggc tcattgtaaa tcacattcaa gatctgatca gcctttccca
    10681 cgagcctcca gtacaggact tcatcagtgc cgttcatcgg aactctgctg ccagcggcct
    10741 gttcatccag gcaattcagt ctcgttgtga aaacctttca actccaacca tgctgaagaa
    10801 aactcttcag tgcttggagg ggatccatct cagccagtcg ggagctgtgc tcacgctgta
    10861 tgtggacagg cttctgtgca cccctttccg tgtgctggct cgcatggtcg acatccttgc
    10921 ttgtcgccgg gtagaaatgc ttctggctgc aaatttacag agcagcatgg cccagttgcc
    10981 aatggaagaa ctcaacagaa tccaggaata ccttcagagc agcgggctcg ctcagagaca
    11041 ccaaaggctc tattccctgc tggacaggtt tcgtctctcc accatgggcg ggtccgggga
    11101 ttacaaggat gacgacgata agtgaaacct ctacttccaa tcgcaccatc atcaccatca
    11161 ccatcaccac catgattaca aggatgacga cgataagtga ggcggccgcc aacttgatat
    11221 caagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
    11281 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
    11341 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
    11401 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
    11461 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
    11521 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
    11581 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt
    11641 tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
    11701 cccttcggcc ctcaatccag cggaccttcc ttcccgcggg cgcccccctc ctcacggcga
    11761 gcgaagcttg tcgagaagta ctagaggatc tctagagcct gcagtctcga caagcttgtc
    11821 gagaagtact agaggatcat aatcagccat accacatttg tagaggtttt acttgcttta
    11881 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
    11941 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
    12001 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
    12061 tatcatgtct ggatctgatc actgcttgag cctaggagat ccgaaccaga taagtgaaat
    12121 ctagttccaa actattttgt catttttaat tttcgtatta gcttacgacg ctacacccag
    12181 ttcccatcta ttttgtcact cttccctaaa taatccttaa aaactccatt tccacccctc
    12241 ccagttccca actattttgt ccgcccacag cggggcattt ttcttcctgt tatgttttta
    12301 atcaaacatc ctgccaactc catgtgacaa accgtcatct tcggctactt t
//
