LOCUS       sequence-402620-        9829 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral plasmid for tetracycline/doxycycline dependent expression
            of murine Ets2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9829)
  AUTHORS   Kubaczka C, Senner CE, Cierlitza M, Arauzo-Bravo MJ, Kuckenberg P, 
            Peitz M, Hemberger M, Schorle H
  TITLE     Direct Induction of Trophoblast Stem Cells from Murine Fibroblasts.
  JOURNAL   Cell Stem Cell. 2015 Sep 22. pii: S1934-5909(15)00360-4. doi: 
            10.1016/j.stem.2015.08.005.
  PUBMED    26412560
REFERENCE   2  (bases 1 to 9829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2015 Sep 22. pii: S1934-5909(15)00360-4. doi: 
            10.1016/j.stem.2015.08.005."
COMMENT     Sequence Label: pLV-tetO-Ets2
FEATURES             Location/Qualifiers
     source          1..9829
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2610..2628,2629..2651,2652..2670,2671..2693,2694..2712,
                     2713..2735,2736..2754,2755..2777,2778..2796,2797..2819,
                     2820..2838,2839..2861,2862..2880)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2913..2951
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2948..2972
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    4521..5109
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4574..4594)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4992..5003)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5112..5128)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5113..5129)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5634..5814
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5840..5857)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5846..6070
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6116..6544
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6203..6222)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6413..6434
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6553..6573)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6558..6887
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6738..6873
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6800..6819
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6935..6982
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7001..7375
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7505..7626
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7542..7561)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7596..7615
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7675..7691)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7675..7691)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7688..7710)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7699..7715
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7723..7729,7730..7747,7748..7753))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7768..7789
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7906..7923)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8077..8665)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8157..8176)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8836..9627,9628..9696))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9459..9478
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9697..9801)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttatcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc
     2641 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat
     2701 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
     2761 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
     2821 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
     2881 aaagtgaaag tcgagctcgg tacccgggtc gaggtaggcg tgtacggtgg gaggcctata
     2941 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
     3001 acctccatag aagacaccgg gaccgatcca gcctccgcgg ccccgaattc ggcttcgcga
     3061 tgaatgactt tggaatcaag aacatggacc aagtggcccc tgtcgccaac agttttcgtg
     3121 ggacactcaa gcgccagcca gcctttgaca ccttcgatgg ctctctgttt gctgtgctcc
     3181 cttctctcag tgaagatcag acactccaag aagtgcccac gggcctggat tctgtctccc
     3241 atgactcggc cagctgcgag ctgcctttgc tcactccctg cagcaaggca gtgatgagcc
     3301 aagccttaaa agccaccttc agtggcttcc aaaaggagca acgacgtctt ggcatcccca
     3361 aaaacccctg gctgtggagc gagcagcagg tgtgccagtg gcttctctgg gccaccaacg
     3421 agttcagcct ggtgaatgtg aacctgcacc agtttggcat gaacggccag atgctgtgta
     3481 acctcggcaa ggagcgcttc ctggagctgg cgcctgactt tgtgggtgac atcctctggg
     3541 aacatctaga gcagatgatc aaagagaacc aagaaaagac agaagaccaa tatgaggaaa
     3601 actctcacct caacgcggtt cctcattgga tcaacagcaa tacattaggc ttcagcatgg
     3661 aacaggctcc atatggaatg caggcaccaa actaccccaa agacaatctc ctggacagca
     3721 tgtgtccgcc atcggccacg cctgcagctc tgggctctga gctccagatg ttgcccaagt
     3781 ctcggctcaa caccgtcaat gtcaattact gttccatcag ccaggacttc cccagcagca
     3841 acgtgaattt gctcaacaac aattctggaa aacccaagga ccacgactct ccagagaacg
     3901 gtggggacag cttcgagagc tccgactcgc tgctgaggtc ctggaacagc cagtcgtccc
     3961 tactggatgt acagcgggta ccttccttcg agagctttga ggaggactgt agccagtctc
     4021 tgtgcctcag taagctgacc atgtccttca aggactacat ccaagagagg agcgacccag
     4081 tcgagcaagg caaaccagtt attcctgcag cagtactggc tggcttcact ggaagcggac
     4141 caatccagtt gtggcagttt cttctggagc tactctctga caagtcctgt caatctttca
     4201 tcagctggac gggggatgga tgggagttca agcttgctga ccccgatgag gttgcccgcc
     4261 ggtgggggaa gaggaaaaat aaaccaaaga tgaactacga gaagctgagc cggggcttac
     4321 gttactacta cgacaagaac atcatccaca agacttcggg caagcgctac gtgtaccgtt
     4381 tcgtatgtga cctgcagaac ttgctgggct tcactccgga ggaactgcat gccatcctgg
     4441 gcgtccagcc tgatacagaa gactgagggc ctcaggacca ccctgagcca gaagccgaat
     4501 tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg
     4561 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     4621 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     4681 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     4741 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     4801 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     4861 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     4921 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     4981 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     5041 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     5101 cctccccgca tcgataccgt cgacctcgag acctagaaaa acatggagca atcacaagta
     5161 gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg
     5221 tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag
     5281 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     5341 gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct gattggcaga
     5401 actacacacc agggccaggg atcagatatc cactgacctt tggatggtgc tacaagctag
     5461 taccagttga gcaagagaag gtagaagaag ccaatgaagg agagaacacc cgcttgttac
     5521 accctgtgag cctgcatggg atggatgacc cggagagaga agtattagag tggaggtttg
     5581 acagccgcct agcatttcat cacatggccc gagagctgca tccggactgt actgggtctc
     5641 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     5701 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     5761 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagggccc
     5821 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
     5881 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     5941 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
     6001 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
     6061 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg
     6121 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     6181 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     6241 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     6301 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     6361 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     6421 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     6481 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     6541 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     6601 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     6661 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     6721 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     6781 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
     6841 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag
     6901 cttgtatatc cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta
     6961 tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc
     7021 cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct
     7081 cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac
     7141 cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg
     7201 ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg
     7261 ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc
     7321 cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt
     7381 gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     7441 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     7501 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     7561 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     7621 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     7681 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     7741 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     7801 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     7861 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     7921 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     7981 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     8041 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     8101 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     8161 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     8221 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     8281 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     8341 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     8401 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     8461 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     8521 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     8581 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     8641 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     8701 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     8761 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     8821 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     8881 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     8941 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     9001 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     9061 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     9121 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     9181 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     9241 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     9301 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     9361 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     9421 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     9481 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     9541 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     9601 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     9661 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     9721 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     9781 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgac
//
