LOCUS       sequence-402731-         971 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial Expression of D242G VraS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 971)
  TITLE     Abdelaziz VraS Plasmids
REFERENCE   2  (bases 1 to 971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET15-VraS - D242G
FEATURES             Location/Qualifiers
     source          1..971
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(851..871)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
     CDS             complement(887..904)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             929..951
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 tctntcgann gcttctttcg ggctttgtta gcagccggat cctcgagtcg acccgggtta
       61 atcgtcatag ctatcttcct ttgttcagcg gcgccttaac ctcaatacgg gtaccgctgt
      121 ccggcaggct cacaatgtga aaggtcgcgc cgatttccag cgcacgctca cgcatgttct
      181 tcagaccata gctttgttcc agtttctcat caacgttaaa gcccttgccg ttgtcctgga
      241 tacgcagcag caggtaatcg tctttgttga acagttcaac ggtcaccttg gtaccgttgc
      301 tgtgacgcag ggtgttgcta atcgcttcct gggtgatacg aaacaggtga tcctcaatgc
      361 ctttcggcac cttgaaacct tggatttcgt gaaccacttt catcggaact ttcttctgca
      421 gatcaatcac caggtccttg atgccctcgc ccaggctttt atccttcaga cccagcggac
      481 gcaggtgcag cagcagcgca cgcatttcca gttggctatc ctgaaccatt ttctccagaa
      541 tcgggatttg ctggtccagc ggcggttcca gtttggtctc cttaatcgcg ctcagcatca
      601 tgctcgccgc gaacagttgc tggctcacgc tgtcgtgcag ctcacgcgcc agacgttgac
      661 gttcatcctc aatgattttc ttaaccttca cgtcgttgat gttgtagttt tcgttggtca
      721 ggttttgggt tttcagacgc agcttgtgca gctcctggtt cagcggaacc agggtgtgat
      781 acagatccag ggtttcgctg taaatctcga tgttctggtc gttgatgccc acggtttcac
      841 cctccatgca gctctgaaag tacagatcct ccatatggcc gctgctgtga tgatgatgat
      901 gatggctgct gagctcgccc atggtatatc tccttcttaa agttaaacaa aattatttct
      961 agaggggaat t
//
