LOCUS       sequence-402734-        1143 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial Expression of M192I VraS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1143)
  TITLE     Abdelaziz VraS Plasmids
REFERENCE   2  (bases 1 to 1143)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET15-VraS - M192I
FEATURES             Location/Qualifiers
     source          1..1143
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     221..240
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        221..239
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             278..300
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             325..342
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             358..378
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
ORIGIN
        1 gtgacccccc cccccaagga tgggcagcaa ggaaaggcgc ccacagtccc cggccncggg
       61 gcttcccaca taccccgccg aacaaggttc agagccgaag tgcgagccga tnttcccatt
      121 cngtgantcg cgatntaggn ccagcacccc cacctgtggc nccgtgatgc cggccacgat
      181 gcgtccggcg tagaggatcg agatctcgat cccgcgaaat taatacgact cactataggg
      241 gaatgtgagc ggataacaat tccccttcta gaaatatttt gtttaacttt aagaaggaga
      301 tataccatgg gcgagctcag cagccatcat catcatcatc acagcagcgg ccatatggag
      361 gatctgtact ttcagagctg catggagggt gaaaccgtgg gcatcaacga ccagaacatc
      421 gagatttaca gcgaaaccct ggatctgtat cacaccctgg ttccgctgaa ccaggagctg
      481 cacaagctgc gtctgaaaac ccaaaacctg accaacgaaa actacaacat caacgacgtg
      541 aaggttaaga aaatcattga ggatgaacgt caacgtctgg cgcgtgagct gcacgacagc
      601 gtgagccagc aactgttcgc ggcgagcatg atgctgagcg cgattaagga gaccaaactg
      661 gaaccgccgc tggaccagca aatcccgatt ctggagaaaa ttgttcagga tagccaactg
      721 gaaatgcgtg cgctgctgct gcacctgcgt ccgctgggtc tgaaggataa aagcctgggc
      781 gagggcatca aggacctggt gattgatctg cagaagaaag ttccgatgaa agtggttcac
      841 gaaatccaag acttcaaggt gccgaaaggc attgaggatc acctgtttcg tatcacccag
      901 gaagcgatta gcaacaccct gcgtcacagc aacggtacca acgtgaccgt tgaactgttc
      961 aacatagacg attacctgct gctgcgtatc cagtacaacg gccaagggct ttaacgttga
     1021 tgagaaacag gaacaaagcc atggtctgaa taacatgctt gaacgtgcgc tgtacattgg
     1081 cacgacctat cacttaggan nntgccggaa cagctgtcac cggnatgagg ttaaggctcc
     1141 gtt
//
