LOCUS       sequence-402737-        1234 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial Expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1234)
  TITLE     Abdelaziz VraS Plasmids
REFERENCE   2  (bases 1 to 1234)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET15-VraS - L114S/D242G
FEATURES             Location/Qualifiers
     source          1..1234
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(848..868)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
     CDS             complement(884..901)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             926..948
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    963..987
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(987..1006)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(988..1006)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1092..1111)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gggcggtgct tctttcgggc tttgttagca gccggatcct cgagtcgacc cgggttaatc
       61 gtcatagcta tcttcctttg ttcagcggcg ccttaacctc aatacgggta ccgctgtccg
      121 gcaggctcac aatgtgaaag gtcgcgccga tttccagcgc acgctcacgc atgttcttca
      181 gaccatagct ttgttccagt ttctcatcaa cgttaaagcc cttgccgttg tcctggatac
      241 gcagcagcag gtaatcgtct ttgttgaaca gttcaacggt caccttggta ccgttgctgt
      301 gacgcagggt gttgctaatc gcttcctggg tgatacgaaa caggtgatcc tcaatgcctt
      361 tcggcacctt gaaaccttgg atttcgtgaa ccactttcat cggaactttc ttctgcagat
      421 caatcaccag gtccttgatg ccctcgccca ggcttttatc cttcagaccc agcggacgca
      481 ggtgcagcag cagcgcacgc atttccagtt ggctatcctg aaccattttc tccagaatcg
      541 ggatttgctg gtccagcggc ggttccagtt tggtctcctt aatcgcgctc agcatcatgc
      601 tcgccgcgaa cagttgctgg ctcacgctgt cgtgcagctc acgcgccaga cgttgacgtt
      661 catcctcaat gattttctta accttcacgt cgttgatgtt gtagttttcg ttggtcaggt
      721 tttgggtttt cagacgcagc ttgtgcagct cctggttcga cggaaccagg gtgtgataca
      781 gatccagggt ttcgctgtaa atctcgatgt tctggtcgtt gatgcccacg gtttcaccct
      841 ccatgcagct ctgaaagtac agatcctcca tatggccgct gctgtgatga tgatgatgat
      901 ggctgctgag ctcgcccatg gtatatctcc ttcttaaagt taaacaaaat tatttctaga
      961 ggggaattgt tatccgctca caattcccct atagtgagtc gtattaattt cgcgggatcg
     1021 agatctcgat cctctacgcc ggaacgcatc gtggccggca tcaccggcgc ccacaggtgc
     1081 ggttgctggc gcctatatcg ccgacatcac ccatggggga agatcgggct cgccacttcg
     1141 ggntcatgaa cgcttgtttc gggtggggta tggtggcagg cccccgggcc gggggactgt
     1201 tggggccctt tccttgatgg cccatttctt gggg
//
