LOCUS       sequence-402942-        7435 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Talin(DeltaR2-12)-mEmerald.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7435)
  AUTHORS   Liu J, Wang Y, Goh WI, Goh H, Baird MA, Ruehland S, Teo S, Bate N, 
            Critchley DR, Davidson MW, Kanchanawong P
  TITLE     Talin determines the nanoscale architecture of focal adhesions.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Sep 1;112(35):E4864-73. doi: 
            10.1073/pnas.1512025112. Epub 2015 Aug 17.
  PUBMED    26283369
REFERENCE   2  (bases 1 to 7435)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1512025112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-09-1- 1"; volume: "112"; issue: "35"; pages: "E4864-73"
COMMENT     Sequence Label: Talin-T36-Cterminal-mEmerald
FEATURES             Location/Qualifiers
     source          1..7435
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        58..361
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        362..565
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     516..536
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      634..643
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      3373..3382
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3379..3381,3382..3384,3385..4098)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3424..3445)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3685..3704)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4032..4053
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    4220..4341
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4257..4276)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4311..4330
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4348..4803)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4485..4506)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4697..4716
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4830..4934
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4936..5293
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4936..5127
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4959..4979)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5144..5279
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5328..6122
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5328..6122
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5382..5401)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5992..6011
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6310..6329)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6354..6401
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6730..7318
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7219..7238
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
       61 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
      121 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
      181 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
      241 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
      301 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
      361 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
      421 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
      481 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
      541 gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct agcgctaccg
      601 gactcagatc tcgagctcaa gcttcgaatt ctagccacca tggttgcgct ttcgctgaag
      661 attagcattg ggaatgtggt gaagacgatg caatttgagc catctaccat ggtgtacgac
      721 gcctgccgca tgattcgtga gcggatccca gaggccctgg ctggccctcc caacgacttt
      781 gggctctttc tgtcagatga tgaccccaaa aaaggcatct ggctagaggc tgggaaagct
      841 ttggactact acatgctccg aaatggggac accatggagt acagaaaaaa gcagagaccc
      901 ctgaagatac ggatgttaga cggaacggtg aagactatca tggtggatga ctccaagacc
      961 gtcacagaca tgctcatgac catttgtgcc cgaattggta tcaccaacca tgatgagtat
     1021 tcactggttc gagagctgat ggaagaaaag aaagatgagg ggacagggac cctgagaaaa
     1081 gacaagaccc tactgcgaga tgaaaagaag atggagaagc taaagcagaa gttgcacaca
     1141 gacgatgagt tgaactggct ggaccatggg cggacactga gggaacaggg agtggaagag
     1201 catgagacgt tgctgctgcg gagaaagttc ttctactcag accagaatgt ggattcccga
     1261 gaccctgtac agctgaacct tctctatgtg caggcacgag atgacatcct gaatggctcc
     1321 catcctgtct cctttgacaa ggcctgtgaa tttgcaggct tccagtgcca gatccagttt
     1381 ggacctcaca atgaacagaa gcacaaggct ggcttccttg acctgaagga cttcctgccc
     1441 aaggagtatg tgaagcagaa gggagagcgt aagatctttc aggcacacaa gaattgtggg
     1501 cagatgagtg aaattgaggc caaggtacgc tacgtgaagc tggcccgttc cctcaagact
     1561 tacggtgtct ccttcttcct agtcaaggaa aagatgaagg ggaagaataa actggtgccc
     1621 aggctgttgg gcatcactaa ggagtgtgtg atgcgtgtgg atgagaagac caaggaggtg
     1681 attcaggaat ggagcctcac caacatcaaa cgctgggctg cctctcccaa gagctttact
     1741 ctggactttg gagactatca ggatggctac tactcagtac agacgacaga aggcgagcag
     1801 atcgcacagc tcattgctgg ctacatagat atcatcctta agaagaaaaa aagcaaggac
     1861 cattttgggc tggagggaga tgaagagtct actatgttgg aggactcagt gtctcccaaa
     1921 aagtcaacag tccttcagca gcagtacaac cgagtgggga aagtggagca cggctctgtg
     1981 gctctgccag ccatcatgcg ctctggagcc tctggtcctg agaatttcca ggtggggagc
     2041 atgccacctg cccagcagca gatcaccagt ggccagatgc accgaggaca catgccacct
     2101 ctgacttcag cccagcaggc gctcactgga accattaact ccagcatgca ggctgtgcag
     2161 gctgcccagg ccactctgga tgactttgag actctacccc ctcttggcca ggatgctgcc
     2221 tccaaagctt ggcgtaagaa caagatggat gaatcaaagc atgagatcca ctcccaggta
     2281 gatgccatca cagctggcac tgcctctgtg gtaaacctga cagcaggaga tcctgcagag
     2341 acagactata cagcagtggg ctgtgcagtc accaccatct cttccaacct gacggagatg
     2401 tcccgtgggg tgaagctatt ggctgccctg ctggaggatg aaggcggcaa tggccggccc
     2461 cttctgcaag cagcaaaggg ccttgcaggg gctgtgtcgg aactgcttcg cagtgctcag
     2521 cccgccagcg ctgagcctcg tcagaacctg ctgcaggcag ccgggaacgt gggccaggcc
     2581 agtggggagc tgttgcagca aattgagtgg gtggacccag aggaccctac tgtcattgct
     2641 gagaatgagc tcttgggagc cgcagccgcc atcgaggctg cagccaagaa gctggagcag
     2701 ctgaagccca gggccaaacc caaggaggcg gatgagtcct tgaactttga ggaacaaatc
     2761 ctagaagctg ccaagtccat cgctgcagcc accagtgcac tggtaaaggc tgcgtcagca
     2821 gcccagaggg agctggtggc tcaaggaaag gtgggcgcca ttccagccaa tgcactggat
     2881 gatgggcagt ggtcgcaggg cctcatttcc gctgcccgta tggtggctgc ggccaccaac
     2941 aatctgtgtg aagcagctaa cgcagctgtc cagggccatg ctagccagga gaaactcatt
     3001 tcctcagcca agcaagtagc tgcctccaca gctcagctcc tggtagcttg caaggtcaaa
     3061 gcagatcagg actctgaggc aatgaaacgg ctccaggctg ctggcaatgc agtgaagagg
     3121 gcctcagata acctggtaaa ggcggcccag aaagctgcag ccttcgaaga ccaggagaat
     3181 gagacggtgg tggtgaagga gaaaatggtt gggggcattg cccagattat cgcagcacag
     3241 gaagagatgc ttcggaagga acgagagctg gaagaggctc ggaaaaagct cgcccagatc
     3301 cggcagcagc agtacaagtt cttgccttca gagcttcgag acgagcacgg ctccgcgtcc
     3361 cgatcaccgg tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     3421 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
     3481 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     3541 cccgtgccct ggcccaccct cgtgaccacc ttgacctacg gcgtgcagtg cttcgcccgc
     3601 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     3661 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     3721 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     3781 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaaggt ctatatcacc
     3841 gccgacaagc agaagaacgg catcaaggtg aacttcaaga cccgccacaa catcgaggac
     3901 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     3961 ctgctgcccg acaaccacta cctgagcacc cagtccaagc tgagcaaaga ccccaacgag
     4021 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     4081 gacgagctgt acaagtaagc ggccgcgact ctagatcata atcagccata ccacatttgt
     4141 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
     4201 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
     4261 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     4321 caaactcatc aatgtatctt aaggcgtaaa ttgtaagcgt taatattttg ttaaaattcg
     4381 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
     4441 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
     4501 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
     4561 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag
     4621 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
     4681 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
     4741 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
     4801 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     4861 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     4921 gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg
     4981 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     5041 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     5101 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     5161 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     5221 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     5281 aggcttttgc aaagatcgat caagagacag gatgaggatc gtttcgcatg attgaacaag
     5341 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
     5401 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
     5461 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag
     5521 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
     5581 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
     5641 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
     5701 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
     5761 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
     5821 tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg
     5881 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
     5941 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
     6001 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
     6061 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
     6121 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga
     6181 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
     6241 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accctagggg
     6301 gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat
     6361 aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc
     6421 ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt
     6481 ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa
     6541 cgtcggggcg gcaggccctg ccatagcctc aggttactca tatatacttt agattgattt
     6601 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     6661 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     6721 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     6781 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     6841 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     6901 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     6961 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     7021 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     7081 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     7141 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     7201 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     7261 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     7321 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     7381 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgccatgc attag
//
