LOCUS       sequence-402983-       10988 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tet-inducible lentiviral plasmid expressing NR4A1-P2A-mCherry, with 
            ORF specific barcode close to 3'LTR site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10988)
  AUTHORS   Obradovic A, Ager C, Turunen M, Nirschl T, Khosravi-Maharlooei M, 
            Iuga A, Jackson CM, Yegnasubramanian S, Tomassoni L, Fernandez EC, 
            McCann P, Rogava M, DeMarzo AM, Kochel CM, Allaf M, Bivalacqua T, 
            Lim M, Realubit R, Karan C, Drake CG, Califano A
  TITLE     Systematic elucidation and pharmacological targeting of 
            tumor-infiltrating regulatory T cell master regulators.
  JOURNAL   Cancer Cell. 2023 May 8;41(5):933-949.e11. doi: 
            10.1016/j.ccell.2023.04.003. Epub 2023 Apr 27.
  PUBMED    37116491
REFERENCE   2  (bases 1 to 10988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2023.04.003"; journalName: "Cancer Cell"; date: 
            "2023-05-8- 8"; volume: "41"; issue: "5"; pages: "933-949.e11"
COMMENT     Sequence Label: Tet-O-FUW-NR4A1-P2A-mCherry
FEATURES             Location/Qualifiers
     source          1..10988
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(50..69)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        241..620
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        622..820
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             838..1018
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1065..1190
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1683..1916
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2101..2145
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2443..2560
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2617..2635
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2659..2677
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2701..2719
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2743..2761
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2785..2803
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2827..2845
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        2878..2916
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2913..2937
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4841..4897
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4907..5614
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(5049..5067)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5317..5336
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    5637..6225
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5690..5710)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6108..6119)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6228..6244)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6229..6245)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6796..6976
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7002..7019)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7008..7232
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7278..7706
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7365..7384)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7575..7596
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7715..7735)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7720..8049
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7900..8035
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        8097..8144
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8163..8537
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8667..8788
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8704..8723)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8758..8777
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8837..8853)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8837..8853)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8850..8872)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8861..8877
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8885..8891,8892..8909,8910..8915))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8930..8951
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9068..9085)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9239..9827)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9319..9338)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9998..10789,10790..10858))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10621..10640
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10859..10963)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct
       61 ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt
      121 agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga
      181 atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt
      241 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
      301 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      361 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      421 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      481 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      541 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      601 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      661 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      721 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      781 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      841 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      901 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      961 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     1021 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     1081 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     1141 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     1201 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1261 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1321 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1381 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1441 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1501 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     1561 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1621 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1681 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1741 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1801 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1861 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1921 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1981 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     2041 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     2101 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     2161 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     2221 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2281 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2341 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     2401 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2461 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2521 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2581 cagagatcca gtttggttag atctcgagtt taccactccc tatcagtgat agagaaaagt
     2641 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac
     2701 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
     2761 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
     2821 taccactccc tatcagtgat agagaaaagt ggaagtcgag ctcggtaccc gggtcgaggt
     2881 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
     2941 tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg atccagcctc
     3001 cgcggccccg aattccgcca ccatgccctg tatccaagcc caatatggga caccagcacc
     3061 gagtccggga ccccgtgacc acctggcaag cgaccccctg acccctgagt tcatcaagcc
     3121 caccatggac ctggccagcc ccgaggcagc ccccgctgcc cccactgccc tgcccagctt
     3181 cagcaccttc atggacggct acacaggaga gtttgacacc ttcctctacc agctgccagg
     3241 aacagtccag ccatgctcct cagcctcctc ctcggcctcc tccacatcct cgtcctcagc
     3301 cacctcccct gcctctgcct ccttcaagtt cgaggacttc caggtgtacg gctgctaccc
     3361 cggccccctg agcggcccag tggatgaggc cctgtcctcc agtggctctg actactatgg
     3421 cagcccctgc tcggccccgt cgccctccac gcccagcttc cagccgcccc agctctctcc
     3481 ctgggatggc tccttcggcc acttctcgcc cagccagact tacgaaggcc tgcgggcatg
     3541 gacagagcag ctgcccaaag cctctgggcc cccacagcct ccagccttct tttccttcag
     3601 tcctcccacc ggccccagcc ccagcctggc ccagagcccc ctgaagttgt tcccctcaca
     3661 ggccacccac cagctggggg agggagagag ctattccatg cctacggcct tcccaggttt
     3721 ggcacccact tctccacacc ttgagggctc ggggatactg gatacacccg tgacctcaac
     3781 caaggcccgg agcggggccc caggtggaag tgaaggccgc tgtgctgtgt gtggggacaa
     3841 cgcttcatgc cagcattatg gtgtccgcac atgtgagggc tgcaagggct tcttcaagcg
     3901 cacagtgcag aaaaacgcca agtacatctg cctggctaac aaggactgcc ctgtggacaa
     3961 gaggcggcga aaccgctgcc agttctgccg cttccagaag tgcctggcgg tgggcatggt
     4021 gaaggaagtt gtccgaacag acagcctgaa ggggcggcgg ggccggctac cttcaaaacc
     4081 caagcagccc ccagatgcct cccctgccaa tctcctcact tccctggtcc gtgcacacct
     4141 ggactcaggg cccagcactg ccaaactgga ctactccaag ttccaggagc tggtgctgcc
     4201 ccactttggg aaggaagatg ctggggatgt acagcagttc tacgacctgc tctccggttc
     4261 tctggaggtc atccgcaagt gggcggagaa gatccctggc tttgctgagc tgtcaccggc
     4321 tgaccaggac ctgttgctgg agtcggcctt cctggagctc ttcatcctcc gcctggcgta
     4381 caggtctaag ccaggcgagg gcaagctcat cttctgctca ggcctggtgc tacaccggct
     4441 gcagtgtgcc cgtggcttcg gggactggat tgacagtatc ctggccttct caaggtccct
     4501 gcacagcttg cttgtcgatg tccctgcctt cgcctgcctc tctgcccttg tcctcatcac
     4561 cgaccggcat gggctgcagg agccgcggcg ggtggaggag ctgcagaacc gcatcgccag
     4621 ctgcctgaag gagcacgtgg cagctgtggc gggcgagccc cagccagcca gctgcctgtc
     4681 acgtctgttg ggcaaactgc ccgagctgcg gaccctgtgc acccagggcc tgcagcgcat
     4741 cttctacctc aagctggagg acttggtgcc ccctccaccc atcattgaca agatcttcat
     4801 ggacacgctg cccttcacgc gtgaattcga tggatccggg gccaccaact tttccctgct
     4861 gaagcaggct ggcgacgtcg aggagaaccc tggccctggc tccggagtga gcaagggcga
     4921 ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc
     4981 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac
     5041 ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct
     5101 gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga
     5161 ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga
     5221 cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa
     5281 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat
     5341 gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat
     5401 caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta
     5461 caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat
     5521 cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca
     5581 ctccaccggc ggcatggacg agctgtacaa gtgaatctag aacgcgttaa gtcgacaatc
     5641 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     5701 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     5761 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     5821 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     5881 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     5941 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     6001 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
     6061 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     6121 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     6181 ttcgccctca gacgagtcgg atctcccttt gggccgcttc cccgcatcga taccgtcgac
     6241 ctcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg
     6301 attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg
     6361 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     6421 aggggggact ggaagggcta attcactccc aacgaagaca agatatcctt gatctgtgga
     6481 tctaccacac acaaggctac ttccctgatt ggcagaacta cacaccaggg ccagggatca
     6541 gatatccact gacctttgga tggtgctaca aggctagkkr mrmkmyycac ggttgcgcgt
     6601 ccgtatcaac tagcagaact agtaccagtt gagcaagaga aggtagaaga agccaatgaa
     6661 ggagagaaca cccgcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga
     6721 gaagtattag agtggaggtt tgacagccgc ctagcatttc atcacatggc ccgagagctg
     6781 catccggact gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     6841 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     6901 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     6961 tggaaaatct ctagcagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag
     7021 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
     7081 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
     7141 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
     7201 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg
     7261 ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     7321 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     7381 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     7441 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     7501 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     7561 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     7621 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     7681 gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga
     7741 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     7801 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     7861 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     7921 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     7981 gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     8041 ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca gcacgtgttg
     8101 acaattaatc atcggcatag tatatcggca tagtataata cgacaaggtg aggaactaaa
     8161 ccatggccaa gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg
     8221 tcgagttctg gaccgaccgg ctcgggttct cccgggactt cgtggaggac gacttcgccg
     8281 gtgtggtccg ggacgacgtg accctgttca tcagcgcggt ccaggaccag gtggtgccgg
     8341 acaacaccct ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg
     8401 aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc catgaccgag atcggcgagc
     8461 agccgtgggg gcgggagttc gccctgcgcg acccggccgg caactgcgtg cacttcgtgg
     8521 ccgaggagca ggactgacac gtgctacgag atttcgattc caccgccgcc ttctatgaaa
     8581 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
     8641 tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat
     8701 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     8761 gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga
     8821 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     8881 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     8941 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     9001 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     9061 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     9121 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     9181 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     9241 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     9301 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     9361 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     9421 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     9481 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     9541 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     9601 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     9661 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     9721 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     9781 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     9841 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     9901 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     9961 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
    10021 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
    10081 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
    10141 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
    10201 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
    10261 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
    10321 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
    10381 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
    10441 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
    10501 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
    10561 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
    10621 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
    10681 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
    10741 cacccaactg atcttcagca tcttttactt ttaccagcgt ttctgggtga gcaaaaacag
    10801 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
    10861 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
    10921 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
    10981 tgccacct
//
