LOCUS       sequence-403113-       15078 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses D498N mutant Homo sapiens ATP13A3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15078)
  AUTHORS   Houdou M, Jacobs N, Coene J, Azfar M, Vanhoutte R, Van den Haute C, 
            Eggermont J, Daniels V, Verhelst SHL, Vangheluwe P
  TITLE     Novel Green Fluorescent Polyamines to Analyze ATP13A2 and ATP13A3 
            Activity in the Mammalian Polyamine Transport System.
  JOURNAL   Biomolecules. 2023 Feb 9;13(2):337. doi: 10.3390/biom13020337.
  PUBMED    36830711
REFERENCE   2  (bases 1 to 15078)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biomolecules."; date: "2023-02-9"; volume: "13(2):337. doi"; pages:
            " 10.3390/biom13020337"
COMMENT     Sequence Label: pLenti HsATP13A3 D498N
FEATURES             Location/Qualifiers
     source          1..15078
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1299..1532
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1717..1761
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2054..2171
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2230..2533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2534..2737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2688..2708
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2734..2758
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2826..2835
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    6566..7118
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6710..6727)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6937..6956
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7118..7720
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MGTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     7617..7637
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7740..8328
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7793..7813)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8211..8222)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8498..8678
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(10206..10225)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(10439..10458)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10558..10580
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10618..10636)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10704..10808
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10809..10877,10878..11669)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11027..11046)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11840..12428
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12329..12348
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12582..12599
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(12669..12689)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        12674..13003
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      12854..12989
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          14137..14202
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             14332..14352
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    14777..14911
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(14814..14833)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     14868..14887
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     1201 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     1261 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     1321 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     1381 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     1441 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     1501 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     1561 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     1621 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     1681 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     1741 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     1801 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     1861 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     1921 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     1981 gacagatcca ttcgattagt gaacggatct cgacggtatc gccgaattca caaatggcag
     2041 tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
     2101 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
     2161 ttcaaaattt tcgggtttat tacagggaca gcagagatcc actttggggc gataagcttg
     2221 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     2281 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     2341 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     2401 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     2461 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     2521 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     2581 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     2641 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
     2701 taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc
     2761 ctggagacgc catccacgct gttttgacct ccatagaaga caccgactct agaggatcca
     2821 ccggtgccac catggacagg gaagaaagga agaccatcaa tcagggtcaa gaagatgaaa
     2881 tggagattta tggttacaat ttgagtcgct ggaagcttgc catagtttct ttaggagtga
     2941 tttgctctgg tgggtttctc ctcctcctcc tctattggat gcctgagtgg cgggtgaaag
     3001 cgacctgtgt cagagctgca attaaagact gtgaagtagt gctgctgagg actactgatg
     3061 aattcaaaat gtggttttgt gcaaaaattc gcgttctttc tttggaaact tacccagttt
     3121 caagtccaaa atctatgtct aataagcttt caaatggcca tgcagtttgt ttaattgaga
     3181 atcccactga agaaaatagg cacaggatca gtaaatattc acagactgaa tcacaacaga
     3241 ttcgttattt cacccaccat agtgtaaaat atttctggaa tgataccatt cacaattttg
     3301 atttcttaaa gggactggat gaaggtgttt cttgtacgtc aatttatgaa aagcatagtg
     3361 caggactgac aaaggggatg catgcctaca gaaaactgct ttatggagta aatgaaattg
     3421 ctgtaaaagt gccttctgtt tttaagcttc taattaaaga ggttctcaac ccattttaca
     3481 ttttccagct gttcagtgtt atactgtgga gcactgatga atactattac tatgctctag
     3541 ctattgtggt tatgtccata gtatcaatcg taagctcact atattccatt agaaagcaat
     3601 atgttatgtt gcatgacatg gtggcaactc atagtaccgt aagagtttca gtttgtagag
     3661 taaatgaaga aatagaagaa atcttttcta ccgaccttgt gccaggagat gtcatggtca
     3721 ttccattaaa tgggacaata atgccttgtg atgctgtgct tattaatggt acctgcattg
     3781 taaacgaaag catgttaaca ggagaaagtg ttccagtgac aaagactaat ttgccaaatc
     3841 cttcagtgga tgtgaaagga ataggagatg aattatataa tccagaaaca cataaacgac
     3901 atactttgtt ttgtgggaca actgttattc agactcgttt ctacactgga gaactcgtca
     3961 aagccatagt tgttagaaca ggatttagta cttccaaagg acagcttgtt cgttccatat
     4021 tgtatcccaa accaactgat tttaaactct acagagatgc ctacttgttt ctactatgtc
     4081 ttgtggcagt tgctggcatt gggtttatct acactattat taatagcatt ttaaatgagg
     4141 tacaagttgg ggtcataatt atcgagtctc ttgatattat cacaattact gtgccccctg
     4201 cacttcctgc tgcaatgact gctggtattg tgtatgctca gagaagactg aaaaaaatcg
     4261 gtattttctg tatcagtcct caaagaataa atatttgtgg acagctcaat cttgtttgct
     4321 ttaacaagac tggaactcta actgaagatg gtttagatct ttgggggatt caacgagtgg
     4381 aaaatgcacg atttctttca ccagaagaaa atgtgtgcaa tgagatgttg gtaaaatccc
     4441 agtttgttgc ttgtatggct acttgtcatt cacttacaaa aattgaagga gtgctctctg
     4501 gtgatccact tgatctgaaa atgtttgagg ctattggatg gattctggaa gaagcaactg
     4561 aagaagaaac agcacttcat aatcgaatta tgcccacagt ggttcgtcct cccaaacaac
     4621 tgcttcctga atctacccct gcaggaaacc aagaaatgga gctgtttgaa cttccagcta
     4681 cttatgagat aggaattgtt cgccagttcc cattttcttc tgctttgcaa cgtatgagtg
     4741 tggttgccag ggtgctgggg gataggaaaa tggacgccta catgaaagga gcgcccgagg
     4801 ccattgccgg tctctgtaaa cctgaaacag ttcctgtcga ttttcaaaac gttttggaag
     4861 acttcactaa acagggcttc cgtgtgattg ctcttgcaca cagaaaattg gagtcaaaac
     4921 tgacatggca taaagtacag aatattagca gagatgcaat tgagaacaac atggatttta
     4981 tgggattaat tataatgcag aacaaattaa agcaagaaac ccctgcagta cttgaagatt
     5041 tgcataaagc caacattcgc accgtcatgg tcacaggtga cagtatgttg actgctgtct
     5101 ctgtggccag agattgtgga atgattctac ctcaggataa agtgattatt gctgaagcat
     5161 tacctccaaa ggatgggaaa gttgccaaaa taaattggca ttatgcagac tccctcacgc
     5221 agtgcagtca tccatcagca attgacccag aggctattcc ggttaaattg gtccatgata
     5281 gcttagagga tcttcaaatg actcgttatc attttgcaat gaatggaaaa tcattctcag
     5341 tgatactgga gcattttcaa gaccttgttc ctaagttgat gttgcatggc accgtgtttg
     5401 cccgtatggc acctgatcag aagacacagt tgatagaagc attgcaaaat gttgattatt
     5461 ttgttgggat gtgtggtgat ggcgcaaatg attgtggtgc tttgaagagg gcacacggag
     5521 gcatttcctt atcggagctc gaagcttcag tggcatctcc ctttacctct aagactccta
     5581 gtatttcctg tgtgccaaac cttatcaggg aaggccgtgc tgctttaata acttccttct
     5641 gtgtgtttaa attcatggca ttgtacagca ttatccagta cttcagtgtt actctgctgt
     5701 attctatctt aagtaaccta ggagacttcc agtttctctt cattgatctg gcaatcattt
     5761 tggtagtggt atttacaatg agtttaaatc ctgcctggaa agaacttgtg gcacaaagac
     5821 caccttcggg tcttatatct ggggcccttc tcttctccgt tttgtctcag attatcatct
     5881 gcattggatt tcaatctttg ggtttttttt gggtcaaaca gcaaccttgg tatgaagtgt
     5941 ggcatccaaa atcagatgct tgtaatacaa caggaagcgg gttttggaat tcttcacacg
     6001 tagacaatga aaccgaactt gatgaacata atatacaaaa ttatgaaaat accacagtgt
     6061 tttttatttc cagttttcag tacctcatag tggcaattgc cttttcaaaa ggaaaaccct
     6121 tcaggcaacc ttgctacaaa aattattttt ttgttttttc tgtgattttt ttatatattt
     6181 ttatattatt catcatgttg tatccagttg cctctgttga ccaggttctt cagatagtgt
     6241 gtgtaccata tcagtggcgt gtaactatgc tcatcattgt tcttgtcaat gcctttgtgt
     6301 ctatcacagt ggaggagtca gtggatcggt ggggaaaatg ctgcttaccc tgggccctgg
     6361 gctgtagaaa gaagacacca aaggcaaagt acatgtatct ggcgcaggag ctcttggttg
     6421 atccagaatg gccaccaaaa cctcagacaa ccacagaagc taaagcttta gttaaggaga
     6481 atggatcatg tcaaatcatc accataacat agctcgagac tagtacgcgt gctctagccc
     6541 aattccgccc cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg
     6601 tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc
     6661 cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa
     6721 ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga
     6781 caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc
     6841 ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc
     6901 cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac
     6961 aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg
     7021 tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac
     7081 ggggacgtgg ttttcctttg aaaaacacga tgataatatg ggcaccgagt acaagcccac
     7141 ggtgcgcctc gccacccgcg acgacgtccc ccgggccgta cgcaccctcg ccgccgcgtt
     7201 cgccgactac cccgccacgc gccacaccgt cgacccggac cgccacatcg agcgggtcac
     7261 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
     7321 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
     7381 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
     7441 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
     7501 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
     7561 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
     7621 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
     7681 accgcgcacc tggtgcatga cccgcaagcc cggtgcctga ggcgcgttga attcccgata
     7741 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     7801 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     7861 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     7921 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     7981 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     8041 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     8101 tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg
     8161 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     8221 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     8281 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat cgggaattct
     8341 gtcgactggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     8401 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa aatcgtcgag
     8461 agatgctgca tataagcagc tgctttttgc ttgtactggg tctctctggt tagaccagat
     8521 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     8581 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     8641 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
     8701 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagaggc cttgacatta
     8761 taatagattt agcaggaatt gaactaggag tggagcacac aggcaaagct gcagaagtac
     8821 ttggaagaag ccaccagaga tactcacgat tctgcacata cctggctaat cccagatcct
     8881 aaggattaca ttaagtttac taacatttat ataatgattt atagtttaaa gtataaactt
     8941 atctaattta ctattctgac agatattaat taatcctcaa atatcataag agatgattac
     9001 tattatcccc atttaacaca agaggaaact gagagggaaa gatgttgaag taattttccc
     9061 acaattacag catccgttag ttacgactct atgatcttct gacacaaatt ccatttactc
     9121 ctcaccctat gactcagtcg aatatatcaa agttatggac attatgctaa gtaacaaatt
     9181 acccttttat atagtaaata ctgagtagat tgagagaaga aattgtttgc aaacctgaat
     9241 agcttcaaga agaagagaag tgaggataag aataacagtt gtcatttaac aagttttaac
     9301 aagtaacttg gttagaaagg gattcaaatg cataaagcaa gggataaatt tttctggcaa
     9361 caagactata caatataacc ttaaatatga cttcaaataa ttgttggaac ttgataaaac
     9421 taattaaata ttattgaaga ttatcaatat tataaatgta atttactttt aaaaagggaa
     9481 catagaaatg tgtatcatta gagtagaaaa caatccttat tatcacaatt tgtcaaaaca
     9541 agtttgttat taacacaagt agaatactgc attcaattaa gttgactgca gattttgtgt
     9601 tttgttaaaa ttagaaagag ataacaacaa tttgaattat tgaaagtaac atgtaaatag
     9661 ttctacatac gttcttttga catcttgttc aatcattgat cgaagttctt tatcttggaa
     9721 gaatttgttc caaagactct gaaataagga aaacaatcta ttatatagtc tcacaccttt
     9781 gttttacttt tagtgatttc aatttaataa tgtaaatggt taaaatttat tcttctctga
     9841 gatcatttca cattgcagat agaaaacctg agactggggt aatttttatt aaaatctaat
     9901 ttaatctcag aaacacatct ttattctaac atcaattttt ccagtttgat attatcatat
     9961 aaagtcagcc ttcctcatct gcaggttcca caacaaaaat ccaaccaact gtggatcaaa
    10021 aatattggga aaaaattaaa aatagcaata caacaataaa aaaatacaaa tcagaaaaac
    10081 agcacagtat aacaacttta tttagcattt acaatctatt aggtattata agtaatctag
    10141 ccagatcctc tacgccggac gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc
    10201 tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg gctcgccact tcgggctcat
    10261 gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg gccgggggac tgttgggcgc
    10321 catctccttg catgcaccat tccttgcggc ggcggtgctc aacggcctca acctactact
    10381 gggctgcttc ctaatgcagg agtcgcataa gggagagcgt cgaatggtgc actctcagta
    10441 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
    10501 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
    10561 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc
    10621 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
    10681 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
    10741 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
    10801 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
    10861 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
    10921 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
    10981 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
    11041 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
    11101 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
    11161 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
    11221 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
    11281 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
    11341 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
    11401 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
    11461 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
    11521 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
    11581 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
    11641 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
    11701 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
    11761 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
    11821 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
    11881 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
    11941 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
    12001 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
    12061 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
    12121 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
    12181 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
    12241 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
    12301 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
    12361 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
    12421 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
    12481 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
    12541 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
    12601 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
    12661 gcagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
    12721 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
    12781 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
    12841 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
    12901 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
    12961 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagcttg gacacaagac
    13021 aggcttgcga gatatgtttg agaataccac tttatcccgc gtcagggaga ggcagtgcgt
    13081 aaaaagacgc ggactcatgt gaaatactgg tttttagtgc gccagatctc tataatctcg
    13141 cgcaacctat tttcccctcg aacacttttt aagccgtaga taaacaggct gggacacttc
    13201 acatgagcga aaaatacatc gtcacctggg acatgttgca gatccatgca cgtaaactcg
    13261 caagccgact gatgccttct gaacaatgga aaggcattat tgccgtaagc cgtggcggtc
    13321 tgtaccgggt gcgttactgg cgcgtgaact gggtattcgt catgtcgata ccgtttgtat
    13381 ttccagctac gatcacgaca accagcgcga gcttaaagtg ctgaaacgcg cagaaggcga
    13441 tggcgaaggc ttcatcgtta ttgatgacct ggtggatacc ggtggtactg cggttgcgat
    13501 tcgtgaaatg tatccaaaag cgcactttgt caccatcttc gcaaaaccgg ctggtcgtcc
    13561 gctggttgat gactatgttg ttgatatccc gcaagatacc tggattgaac agccgtggga
    13621 tatgggcgtc gtattcgtcc cgccaatctc cggtcgctaa tcttttcaac gcctggcact
    13681 gccgggcgtt gttcttttta acttcaggcg ggttacaata gtttccagta agtattctgg
    13741 aggctgcatc catgacacag gcaaacctga gcgaaaccct gttcaaaccc cgctttaaac
    13801 atcctgaaac ctcgacgcta gtccgccgct ttaatcacgg cgcacaaccg cctgtgcagt
    13861 cggcccttga tggtaaaacc atccctcact ggtatcgcat gattaaccgt ctgatgtgga
    13921 tctggcgcgg cattgaccca cgcgaaatcc tcgacgtcca ggcacgtatt gtgatgagcg
    13981 atgccgaacg taccgacgat gatttatacg atacggtgat tggctaccgt ggcggcaact
    14041 ggatttatga gtgggccccg gatctttgtg aaggaacctt acttctgtgg tgtgacataa
    14101 ttggacaaac tacctacaga gatttaaagc tctaaggtaa atataaaatt tttaagtgta
    14161 taatgtgtta aactactgat tctaattgtt tgtgtatttt agattccaac ctatggaact
    14221 gatgaatggg agcagtggtg gaatgccttt aatgaggaaa acctgttttg ctcagaagaa
    14281 atgccatcta gtgatgatga ggctactgct gactctcaac attctactcc tccaaaaaag
    14341 aagagaaagg tagaagaccc caaggacttt ccttcagaat tgctaagttt tttgagtcat
    14401 gctgtgttta gtaatagaac tcttgcttgc tttgctattt acaccacaaa ggaaaaagct
    14461 gcactgctat acaagaaaat tatggaaaaa tattctgtaa cctttataag taggcataac
    14521 agttataatc ataacatact gttttttctt actccacaca ggcatagagt gtctgctatt
    14581 aataactatg ctcaaaaatt gtgtaccttt agctttttaa tttgtaaagg ggttaataag
    14641 gaatatttga tgtatagtgc cttgactaga gatcataatc agccatacca catttgtaga
    14701 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
    14761 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
    14821 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
    14881 actcatcaat gtatcttatc atgtctggat caactggata actcaagcta accaaaatca
    14941 tcccaaactt cccaccccat accctattac cactgccaat tacctgtggt ttcatttact
    15001 ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg
    15061 tactacaaac ttagtagt
//
