LOCUS       sequence-403313-       11165 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) 
            and B2M sgRNA (human U6 promoter, 5'-GAGTAGCGCGAGCACAGCTA).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11165)
  AUTHORS   Hamilton JR, Chen E, Perez BS, Sandoval Espinoza CR, Kang MH, 
            Trinidad M, Ngo W, Doudna JA
  TITLE     In vivo human T cell engineering with enveloped delivery vehicles.
  JOURNAL   Nat Biotechnol. 2024 Jan 11. doi: 10.1038/s41587-023-02085-z.
  PUBMED    38212493
REFERENCE   2  (bases 1 to 11165)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2024 Jan 11. doi: 10.1038/s41587-023-02085-z."
COMMENT     Sequence Label: pJRH-1346 U6-B2M sgRNA Gag-pol v2
FEATURES             Location/Qualifiers
     source          1..11165
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        84..332
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     84..104
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     255..274
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     enhancer        486..865
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        867..1144
                     /label=chicken beta-actin promoter
     intron          1145..2161
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2085..2107
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2169..2188
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             2267..3769
                     /codon_start=1
                     /gene="gag"
                     /product="gag protein from human immunodeficiency virus 1"
                     /label=HIV-1 gag
                     /translation="MGARASVLSGGELDRWEKIRLRPGGKKKYKLKHIVWASRELERFA
                     VNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTVATLYCVHQRIEIKDTKEALDKI
                     EEEQNKSKKKAQQAAADTGHSNQVSQNYPIVQNIQGQMVHQAISPRTLNAWVKVVEEKA
                     FSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRVHPVHAGP
                     IAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRMYSPTS
                     ILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPG
                     ATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKCFNCGKEG
                     HIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAP
                     PEESFRFGEETTTPSQKQEPIDKELYPLASLRSLFGSDPSSQ"
     CDS             3562..6573
                     /codon_start=1
                     /gene="pol"
                     /product="pol protein from human immunodeficiency virus 1"
                     /label=HIV-1 pol
                     /translation="FFREDLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEAG
                     ADRQGTVSFSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKMIG
                     GIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETV
                     PVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKD
                     STKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKQKKSVTVLDVGDAYFSVPLDKDFRK
                     YTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQCSMTKILEPFRKQNPDIVIYQYMD
                     DLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPI
                     VLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVRQLCKLLRGTKALTEVVPLTEEAELEL
                     AENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMKGAHT
                     NDVKQLTEAVQKIATESIVIWGKTPKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPP
                     LVKLWYQLEKEPIIGAETFYVDGAANRETKLGKAGYVTDRGRQKVVPLTDTTNQKTELQ
                     AIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAH
                     KGIGGNEQVDKLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVA
                     SCDKCQLKGEAMHGQVDCSPGIWQLDCTHLEGKVILVAVHVASGYIEAEVIPAETGQET
                     AYFLLKLAGRWPVKTVHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNK
                     ELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK
                     QITKIQNFRVYYRDSRDPVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQ
                     MAGDDCVASRQDED"
     misc_feature    6258..6375
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    7146..7379
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7564..7608
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             7902..7928
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     primer_bind     complement(8213..8232)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    8278..8333
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8332..8351)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(8694..8710)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8694..8710)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8707..8729)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8718..8734
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8742..8748,8749..8766,8767..8772))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8787..8808
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8867..9062
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8913..9048
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    9068..9202
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9105..9124)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9159..9178
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9270..9287)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9441..10029)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9521..9540)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10200..10991,10992..11060))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10823..10842
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11061..11165)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacatttccc cgaaaagtgc cacctgggtc gaccgggttt attacaggga cagcagagat
       61 ccagtttggt taattaaggt accgagggcc tatttcccat gattccttca tatttgcata
      121 tacgatacaa ggctgttaga gagataatta gaattaattt gactgtaaac acaaagatat
      181 tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat
      241 tatgttttaa aatggactat catatgctta ccgtaacttg aaagtatttc gatttcttgg
      301 ctttatatat cttgtggaaa ggacgaaaca ccggagtagc gcgagcacag ctagtttaag
      361 agctatgctg gaaacagcat agcaagttta aataaggcta gtccgttatc aacttgaaaa
      421 agtggcaccg agtcggtgct ttttttgaat tcgctagcta ggtcttgaaa ggagtgggaa
      481 ttgtcgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
      541 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
      601 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
      661 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
      721 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
      781 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
      841 cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc
      901 catctccccc ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc
      961 agcgatgggg gcgggggggg ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg
     1021 gcggggcggg gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa
     1081 gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg
     1141 ggcgggagtc gctgcgcgct gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg
     1201 cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc
     1261 tcctccgggc tgtaattagc gcttggttta atgacggctt gtttcttttc tgtggctgcg
     1321 tgaaagcctt gaggggctcc gggagggccc tttgtgcggg gggagcggct cggggggtgc
     1381 gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga
     1441 gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc
     1501 cgggggcggt gccccgcggt gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg
     1561 tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct
     1621 gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg
     1681 gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg
     1741 ggcggggccg cctcgggccg gggagggctc gggggagggg cgcggcggcc cccggagcgc
     1801 cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat cgtgcgagag
     1861 ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga ggcgccgccg
     1921 caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg
     1981 ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc ctcggggctg
     2041 tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg
     2101 tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct ttttcctaca
     2161 gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag aattcgggcc
     2221 gcgttgacgc gcacggcaag aggcgagggg cggcgactgg tgagagatgg gtgcgagagc
     2281 gtcagtatta agcgggggag aattagatcg atgggaaaaa attcggttaa ggccaggggg
     2341 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
     2401 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
     2461 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
     2521 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
     2581 ggaagagcaa aacaaaagta agaaaaaagc acagcaagca gcagctgaca caggacacag
     2641 caatcaggtc agccaaaatt accctatagt gcagaacatc caggggcaaa tggtacatca
     2701 ggccatatca cctagaactt taaatgcatg ggtaaaagta gtagaagaga aggctttcag
     2761 cccagaagtg atacccatgt tttcagcatt atcagaagga gccaccccac aagatttaaa
     2821 caccatgcta aacacagtgg ggggacatca agcagccatg caaatgttaa aagagaccat
     2881 caatgaggaa gctgcagaat gggatagagt gcatccagtg catgcagggc ctattgcacc
     2941 aggccagatg agagaaccaa ggggaagtga catagcagga actactagta cccttcagga
     3001 acaaatagga tggatgacac ataatccacc tatcccagta ggagaaatct ataaaagatg
     3061 gataatcctg ggattaaata aaatagtaag aatgtatagc cctaccagca ttctggacat
     3121 aagacaagga ccaaaggaac cctttagaga ctatgtagac cgattctata aaactctaag
     3181 agccgagcaa gcttcacaag aggtaaaaaa ttggatgaca gaaaccttgt tggtccaaaa
     3241 tgcgaaccca gattgtaaga ctattttaaa agcattggga ccaggagcga cactagaaga
     3301 aatgatgaca gcatgtcagg gagtgggggg acccggccat aaagcaagag ttttggctga
     3361 agcaatgagc caagtaacaa atccagctac cataatgata cagaaaggca attttaggaa
     3421 ccaaagaaag actgttaagt gtttcaattg tggcaaagaa gggcacatag ccaaaaattg
     3481 cagggcccct aggaaaaagg gctgttggaa atgtggaaag gaaggacacc aaatgaaaga
     3541 ttgtactgag agacaggcta attttttagg gaagatctgg ccttcccaca agggaaggcc
     3601 agggaatttt cttcagagca gaccagagcc aacagcccca ccagaagaga gcttcaggtt
     3661 tggggaagag acaacaactc cctctcagaa gcaggagccg atagacaagg aactgtatcc
     3721 tttagcttcc ctcagatcac tctttggcag cgacccctcg tcacaataaa gatagggggg
     3781 caattaaagg aagctctatt agatacagga gcagatgata cagtattaga agaaatgaat
     3841 ttgccaggaa gatggaaacc aaaaatgata gggggaattg gaggttttat caaagtaaga
     3901 cagtatgatc agatactcat agaaatctgc ggacataaag ctataggtac agtattagta
     3961 ggacctacac ctgtcaacat aattggaaga aatctgttga ctcagattgg ctgcacttta
     4021 aattttccca ttagtcctat tgagactgta ccagtaaaat taaagccagg aatggatggc
     4081 ccaaaagtta aacaatggcc attgacagaa gaaaaaataa aagcattagt agaaatttgt
     4141 acagaaatgg aaaaggaagg aaaaatttca aaaattgggc ctgaaaatcc atacaatact
     4201 ccagtatttg ccataaagaa aaaagacagt actaaatgga gaaaattagt agatttcaga
     4261 gaacttaata agagaactca agatttctgg gaagttcaat taggaatacc acatcctgca
     4321 gggttaaaac agaaaaaatc agtaacagta ctggatgtgg gcgatgcata tttttcagtt
     4381 cccttagata aagacttcag gaagtatact gcatttacca tacctagtat aaacaatgag
     4441 acaccaggga ttagatatca gtacaatgtg cttccacagg gatggaaagg atcaccagca
     4501 atattccagt gtagcatgac aaaaatctta gagcctttta gaaaacaaaa tccagacata
     4561 gtcatctatc aatacatgga tgatttgtat gtaggatctg acttagaaat agggcagcat
     4621 agaacaaaaa tagaggaact gagacaacat ctgttgaggt ggggatttac cacaccagac
     4681 aaaaaacatc agaaagaacc tccattcctt tggatgggtt atgaactcca tcctgataaa
     4741 tggacagtac agcctatagt gctgccagaa aaggacagct ggactgtcaa tgacatacag
     4801 aaattagtgg gaaaattgaa ttgggcaagt cagatttatg cagggattaa agtaaggcaa
     4861 ttatgtaaac ttcttagggg aaccaaagca ctaacagaag tagtaccact aacagaagaa
     4921 gcagagctag aactggcaga aaacagggag attctaaaag aaccggtaca tggagtgtat
     4981 tatgacccat caaaagactt aatagcagaa atacagaagc aggggcaagg ccaatggaca
     5041 tatcaaattt atcaagagcc atttaaaaat ctgaaaacag gaaagtatgc aagaatgaag
     5101 ggtgcccaca ctaatgatgt gaaacaatta acagaggcag tacaaaaaat agccacagaa
     5161 agcatagtaa tatggggaaa gactcctaaa tttaaattac ccatacaaaa ggaaacatgg
     5221 gaagcatggt ggacagagta ttggcaagcc acctggattc ctgagtggga gtttgtcaat
     5281 acccctccct tagtgaagtt atggtaccag ttagagaaag aacccataat aggagcagaa
     5341 actttctatg tagatggggc agccaatagg gaaactaaat taggaaaagc aggatatgta
     5401 actgacagag gaagacaaaa agttgtcccc ctaacggaca caacaaatca gaagactgag
     5461 ttacaagcaa ttcatctagc tttgcaggat tcgggattag aagtaaacat agtgacagac
     5521 tcacaatatg cattgggaat cattcaagca caaccagata agagtgaatc agagttagtc
     5581 agtcaaataa tagagcagtt aataaaaaag gaaaaagtct acctggcatg ggtaccagca
     5641 cacaaaggaa ttggaggaaa tgaacaagta gataaattgg tcagtgctgg aatcaggaaa
     5701 gtactatttt tagatggaat agataaggcc caagaagaac atgagaaata tcacagtaat
     5761 tggagagcaa tggctagtga ttttaaccta ccacctgtag tagcaaaaga aatagtagcc
     5821 agctgtgata aatgtcagct aaaaggggaa gccatgcatg gacaagtaga ctgtagccca
     5881 ggaatatggc agctagattg tacacattta gaaggaaaag ttatcttggt agcagttcat
     5941 gtagccagtg gatatataga agcagaagta attccagcag agacagggca agaaacagca
     6001 tacttcctct taaaattagc aggaagatgg ccagtaaaaa cagtacatac agacaatggc
     6061 agcaatttca ccagtactac agttaaggcc gcctgttggt gggcggggat caagcaggaa
     6121 tttggcattc cctacaatcc ccaaagtcaa ggagtaatag aatctatgaa taaagaatta
     6181 aagaaaatta taggacaggt aagagatcag gctgaacatc ttaagacagc agtacaaatg
     6241 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     6301 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     6361 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gaaaggacca
     6421 gcaaagctcc tctggaaagg tgaaggggca gtagtaatac aagataatag tgacataaaa
     6481 gtagtgccaa gaagaaaagc aaagatcatc agggattatg gaaaacagat ggcaggtgat
     6541 gattgtgtgg caagtagaca ggatgaggat taacacatgg aattctgcaa caactgctgt
     6601 ttatccattt cagaattggg ttcgacatag cagaataggc gttactcgac agaggagagc
     6661 aagaaatgga gccagtagat cctagactag agccctggaa gcatccagga agtcagccta
     6721 aaactgcttg taccaattgc tattgtaaaa agtgttgctt tcattgccaa gtttgtttca
     6781 tgacaaaagc cttaggcatc tcctatggca ggaagaagcg gagacagcga cgaagagctc
     6841 atcagaacag tcagactcat caagcttctc tatcaaagca gtaagtagta catgtaatgc
     6901 aacctataat agtagcaata gtagcattag tagtagcaat aataatagca atagttgtgt
     6961 ggtccatagt aatcatagaa tataggaaaa tggccgctga tcttcagacc tggaggagga
     7021 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     7081 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     7141 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     7201 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     7261 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     7321 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     7381 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     7441 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     7501 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     7561 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     7621 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     7681 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     7741 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     7801 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatccttg
     7861 gcacttatct gggacgatct gcggagcctg tgcctcttca gctaccaccg cttgagagac
     7921 ttactcttga ttgtaacgag gattgtggaa cttctgggac gcagggggtg ggaagccctc
     7981 aaatattggt ggaatctcct acaatattgg agtcaggagc taaagaatag tgctgttagc
     8041 ttgctcaatg ccacagccat agcagtagct gaggggacag atagggttat agaagtagta
     8101 caaggagctt gtagagctat tcgccacata cctagaagaa taagacaggg cttggaaagg
     8161 attttgctat aagctcgaaa caaccggtac ctctagaact atagctagca gatctttttc
     8221 cctctgccaa aaattatggg gacatcatga agccccttga gcatctgact tctggctaat
     8281 aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc tcactcggaa
     8341 ggacatatgg gagggcaaat catttaaaac atcagaatga gtatttggtt tagagtttgg
     8401 caacatatgc ccatatgctg gctgccatga acaaaggttg gctataaaga ggtcatcagt
     8461 atatgaaaca gccccctgct gtccattcct tattccatag aaaagccttg acttgaggtt
     8521 agattttttt tatattttgt tttgtgttat ttttttcttt aacatcccta aaattttcct
     8581 tacatgtttt actagccaga tttttcctcc tctcctgact actcccagtc atagctgtcc
     8641 ctcttctctt atggagatcc ctcgacctgc agcccaagct tggcgtaatc atggtcatag
     8701 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     8761 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     8821 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc ggatccgcat ctcaattagt
     8881 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     8941 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     9001 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     9061 aaaagctaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     9121 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     9181 tgtatcttat catgtctgga tccgctgcat taatgaatcg gccaacgcgc ggggagaggc
     9241 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     9301 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     9361 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     9421 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     9481 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     9541 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     9601 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     9661 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     9721 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     9781 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     9841 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     9901 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     9961 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
    10021 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
    10081 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
    10141 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
    10201 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
    10261 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
    10321 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
    10381 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
    10441 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
    10501 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
    10561 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
    10621 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
    10681 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
    10741 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
    10801 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
    10861 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
    10921 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
    10981 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
    11041 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
    11101 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
    11161 ccgcg
//
