LOCUS       sequence-403328-        2289 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 part - TSS promoter (for assembly with level 0 RBS part).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2289)
  TITLE     McCormick Lab Plasmids 2023
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pC0.335
FEATURES             Location/Qualifiers
     source          1..2289
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(12..30)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        98..202
                     /gene="bla"
                     /label=AmpR promoter
     CDS             424..1215
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1386..1974
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1875..1894
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2128..2145
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tggacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
       61 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
      121 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
      181 ataatattga aaaaggaaga gtatgcgctc acgcaactgg tccagaacct tgaccgaacg
      241 cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttttggg
      301 gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg
      361 atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac
      421 atcatgaggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc
      481 atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat
      541 ggcggcctga agccacacag cgatattgat ttgctggtta cggtgaccgt aaggcttgat
      601 gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag
      661 agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg
      721 cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca
      781 ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga
      841 gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa
      901 caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg
      961 gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc
     1021 ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag
     1081 tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg
     1141 gcctcgcgcg cagatcagtt ggaagaattt gtccattacg taaaaggcga gatcaccaag
     1201 gtagtcggca aataactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     1261 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     1321 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     1381 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     1441 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     1501 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     1561 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     1621 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     1681 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     1741 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     1801 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     1861 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     1921 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     1981 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     2041 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     2101 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     2161 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatcact ctgtggtctc
     2221 aggagttgac ggctaggctc agtcctaggt atagtgctag ctactagaga ccacgaagtg
     2281 gctcttcag
//
