LOCUS       sequence-403449-       10539 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Determine where transit peptide targets GFP in N. benthamiana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10539)
  AUTHORS   DeTar RA, Chustecki JM, Martinez-Hottovy A, Ceriotti LF, Broz AK, 
            Lou X, Sanchez-Puerta MV, Elowsky C, Christensen AC, Sloan DB
  TITLE     Photosynthetic demands on translational machinery drive retention of
            redundant tRNA metabolism in plant organelles.
  JOURNAL   Proc Natl Acad Sci U S A. 2024 Dec 24;121(52):e2421485121. doi: 
            10.1073/pnas.2421485121. Epub 2024 Dec 18.
  PUBMED    39693336
REFERENCE   2  (bases 1 to 10539)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2024-12-24"; volume: "121(52):e2421485121. 
            doi"; pages: " 10.1073/pnas.2421485121. Epub 2024 Dec 18"
COMMENT     Sequence Label: Shim_ProRS_TP_GFP_pK7FWG2
FEATURES             Location/Qualifiers
     source          1..10539
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      925..1513
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1414..1433
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1667..1684
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    1699..1839
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(1765..1784)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1925..1947
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      2183..2377
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(2443..3516)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(3945..4574)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    5905..5929
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        6622..6966
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     6910..6930
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    7066..7090
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7373..7397)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(7405..7407,7408..7410,7411..8124)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7405..7407,7408..7410,7411..8121)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7450..7471)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7711..7730)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8058..8079
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        8490..8669
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             8703..9497
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8757..8776)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9367..9386
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      9537..9789
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    9882..9906
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             join(10427..10539,1..679)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct
       61 tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg
      121 agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc
      181 gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct
      241 tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc
      301 aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc
      361 tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga
      421 ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt
      481 aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc
      541 ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg
      601 cttggcctcg cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac
      661 caaggtagtc ggcaaataat gtctagctag aaattcgttc aagccgacgc cgcttcgcgg
      721 cgcggcttaa ctcaagcgtt agatgcacta agcacataat tgctcacagc caaactatca
      781 ggtcaagtct gcttttatta tttttaagcg tgcataataa gccctacaca aattgggaga
      841 tatatcatgc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
      901 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
      961 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     1021 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     1081 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     1141 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     1201 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     1261 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     1321 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     1381 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     1441 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     1501 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     1561 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     1621 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     1681 cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     1741 acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta
     1801 tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc
     1861 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     1921 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag
     1981 ggtgccttga tgtgggcgcc ggcggtcgag tggcgacggc gcggcttgtc cgcgccctgg
     2041 tagattgcct ggccgtaggc cagccatttt tgagcggcca gcggccgcga taggccgacg
     2101 cgaagcggcg gggcgtaggg agcgcagcga ccgaagggta ggcgcttttt gcagctcttc
     2161 ggctgtgcgc tggccagaca gttatgcaca ggccaggcgg gttttaagag ttttaataag
     2221 ttttaaagag ttttaggcgg aaaaatcgcc ttttttctct tttatatcag tcacttacat
     2281 gtgtgaccgg ttcccaatgt acggctttgg gttcccaatg tacgggttcc ggttcccaat
     2341 gtacggcttt gggttcccaa tgtacgtgct atccacagga aagagacctt ttcgaccttt
     2401 ttcccctgct agggcaattt gccctagcat ctgctccgta cattaggaac cggcggatgc
     2461 ttcgccctcg atcaggttgc ggtagcgcat gactaggatc gggccagcct gccccgcctc
     2521 ctccttcaaa tcgtactccg gcaggtcatt tgacccgatc agcttgcgca cggtgaaaca
     2581 gaacttcttg aactctccgg cgctgccact gcgttcgtag atcgtcttga acaaccatct
     2641 ggcttctgcc ttgcctgcgg cgcggcgtgc caggcggtag agaaaacggc cgatgccggg
     2701 atcgatcaaa aagtaatcgg ggtgaaccgt cagcacgtcc gggttcttgc cttctgtgat
     2761 ctcgcggtac atccaatcag ctagctcgat ctcgatgtac tccggccgcc cggtttcgct
     2821 ctttacgatc ttgtagcggc taatcaaggc ttcaccctcg gataccgtca ccaggcggcc
     2881 gttcttggcc ttcttcgtac gctgcatggc aacgtgcgtg gtgtttaacc gaatgcaggt
     2941 ttctaccagg tcgtctttct gctttccgcc atcggctcgc cggcagaact tgagtacgtc
     3001 cgcaacgtgt ggacggaaca cgcggccggg cttgtctccc ttcccttccc ggtatcggtt
     3061 catggattcg gttagatggg aaaccgccat cagtaccagg tcgtaatccc acacactggc
     3121 catgccggcc ggccctgcgg aaacctctac gtgcccgtct ggaagctcgt agcggatcac
     3181 ctcgccagct cgtcggtcac gcttcgacag acggaaaacg gccacgtcca tgatgctgcg
     3241 actatcgcgg gtgcccacgt catagagcat cggaacgaaa aaatctggtt gctcgtcgcc
     3301 cttgggcggc ttcctaatcg acggcgcacc ggctgccggc ggttgccggg attctttgcg
     3361 gattcgatca gcggccgctt gccacgattc accggggcgt gcttctgcct cgatgcgttg
     3421 ccgctgggcg gcctgcgcgg ccttcaactt ctccaccagg tcatcaccca gcgccgcgcc
     3481 gatttgtacc gggccggatg gtttgcgacc gctcacgccg attcctcggg cttgggggtt
     3541 ccagtgccat tgcagggccg gcagacaacc cagccgctta cgcctggcca accgcccgtt
     3601 cctccacaca tggggcattc cacggcgtcg gtgcctggtt gttcttgatt ttccatgccg
     3661 cctcctttag ccgctaaaat tcatctactc atttattcat ttgctcattt actctggtag
     3721 ctgcgcgatg tattcagata gcagctcggt aatggtcttg ccttggcgta ccgcgtacat
     3781 cttcagcttg gtgtgatcct ccgccggcaa ctgaaagttg acccgcttca tggctggcgt
     3841 gtctgccagg ctggccaacg ttgcagcctt gctgctgcgt gcgctcggac ggccggcact
     3901 tagcgtgttt gtgcttttgc tcattttctc tttacctcat taactcaaat gagttttgat
     3961 ttaatttcag cggccagcgc ctggacctcg cgggcagcgt cgccctcggg ttctgattca
     4021 agaacggttg tgccggcggc ggcagtgcct gggtagctca cgcgctgcgt gatacgggac
     4081 tcaagaatgg gcagctcgta cccggccagc gcctcggcaa cctcaccgcc gatgcgcgtg
     4141 cctttgatcg cccgcgacac gacaaaggcc gcttgtagcc ttccatccgt gacctcaatg
     4201 cgctgcttaa ccagctccac caggtcggcg gtggcccata tgtcgtaagg gcttggctgc
     4261 accggaatca gcacgaagtc ggctgccttg atcgcggaca cagccaagtc cgccgcctgg
     4321 ggcgctccgt cgatcactac gaagtcgcgc cggccgatgg ccttcacgtc gcggtcaatc
     4381 gtcgggcggt cgatgccgac aacggttagc ggttgatctt cccgcacggc cgcccaatcg
     4441 cgggcactgc cctggggatc ggaatcgact aacagaacat cggccccggc gagttgcagg
     4501 gcgcgggcta gatgggttgc gatggtcgtc ttgcctgacc cgcctttctg gttaagtaca
     4561 gcgataacct tcatgcgttc cccttgcgta tttgtttatt tactcatcgc atcatatacg
     4621 cagcgaccgc atgacgcaag ctgttttact caaatacaca tcaccttttt agacggcggc
     4681 gctcggtttc ttcagcggcc aagctggccg gccaggccgc cagcttggca tcagacaaac
     4741 cggccaggat ttcatgcagc cgcacggttg agacgtgcgc gggcggctcg aacacgtacc
     4801 cggccgcgat catctccgcc tcgatctctt cggtaatgaa aaacggttcg tcctggccgt
     4861 cctggtgcgg tttcatgctt gttcctcttg gcgttcattc tcggcggccg ccagggcgtc
     4921 ggcctcggtc aatgcgtcct cacggaaggc accgcgccgc ctggcctcgg tgggcgtcac
     4981 ttcctcgctg cgctcaagtg cgcggtacag ggtcgagcga tgcacgccaa gcagtgcagc
     5041 cgcctctttc acggtgcggc cttcctggtc gatcagctcg cgggcgtgcg cgatctgtgc
     5101 cggggtgagg gtagggcggg ggccaaactt cacgcctcgg gccttggcgg cctcgcgccc
     5161 gctccgggtg cggtcgatga ttagggaacg ctcgaactcg gcaatgccgg cgaacacggt
     5221 caacaccatg cggccggccg gcgtggtggt gtcggcccac ggctctgcca ggctacgcag
     5281 gcccgcgccg gcctcctgga tgcgctcggc aatgtccagt aggtcgcggg tgctgcgggc
     5341 caggcggtct agcctggtca ctgtcacaac gtcgccaggg cgtaggtggt caagcatcct
     5401 ggccagctcc gggcggtcgc gcctggtgcc ggtgatcttc tcggaaaaca gcttggtgca
     5461 gccggccgcg tgcagttcgg cccgttggtt ggtcaagtcc tggtcgtcgg tgctgacgcg
     5521 ggcatagccc agcaggccag cggcggcgct cttgttcatg gcgtaatgtc tccggttcta
     5581 gtcgcaagta ttctacttta tgcgactaaa acacgcgaca agaaaacgcc aggaaaaggg
     5641 cagggcggca gcctgtcgcg taacttagga cttgtgcgac atgtcgtttt cagaagacgg
     5701 ctgcactgaa cgtcagaagc cgactgcact atagcagcgg aggggttgga tcaaagtact
     5761 ttaaagtact ttaaagtact ttaaagtact ttgatcccga ggggaaccct gtggttggca
     5821 tgcacataca aatggacgaa cggataaacc ttttcacgcc cttttaaata tccgattatt
     5881 ctaataaacg ctcttttctc ttaggtttac ccgccaatat atcctgtcaa acactgatag
     5941 tttaaactga aggcgggaaa cgacaatctg atccaagctc aagctaagct tgagctctcc
     6001 catatggtcg actagagcca agctgatctc ctttgccccg gagatcacca tggacgactt
     6061 tctctatctc tacgatctag gaagaaagtt cgacggagaa ggtgacgata ccatgttcac
     6121 caccgataat gagaagatta gcctcttcaa tttcagaaag aatgctgacc cacagatggt
     6181 tagagaggcc tacgcggcag gtctcatcaa gacgatctac ccgagtaata atctccagga
     6241 gatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga ctaactgcat
     6301 caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag tatggacgat
     6361 tcaaggcttg cttcataaac caaggcaagt aatagagatt ggagtctcta agaaagtagt
     6421 tcctactgaa tcaaaggcca tggagtcaaa aattcagatc gaggatctaa cagaactcgc
     6481 cgtgaagact ggcgaacagt tcatacagag tcttttacga ctcaatgaca agaagaaaat
     6541 cttcgtcaac atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt
     6601 ctcagaagac caaagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct
     6661 cggattccat tgcccagcta tctgtcactt catcaaaagg acagtagaaa aggaaggtgg
     6721 cacctacaaa tgccatcatt gcgataaagg aaaggctatc gttcaagatg cccctgccga
     6781 cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc
     6841 aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc
     6901 acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga
     6961 gaggactccg gtatttttac aacaattacc acaacaaaac aaacaacaaa caacattaca
     7021 atttactatt ctagtcgacc tgcaggcggc cgcactagtg atatcacaag tttgtacaaa
     7081 aaagcaggct ccgcggccgc ccccttcacc atgaagcttg ctacttatct tagatctctt
     7141 actagagaag ttccatggta tggatcttgg tcttggtctt ctgctgaaag atgtggagtt
     7201 cttccaccat ctgcttctat ttctgattct gcttctactc catctaagca agttacttct
     7261 tctccagttg gagctctttc tcttgaacat atgtcttctg ttggaagaaa gaaggaagtt
     7321 aagaaggaaa ctagacttgg actttctttt aagaagaagg gtgggcgcgc cgacccagct
     7381 ttcttgtaca aagtggtgat atcaatggtg agcaagggcg aggagctgtt caccggggtg
     7441 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     7501 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     7561 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
     7621 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     7681 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     7741 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     7801 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     7861 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
     7921 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     7981 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
     8041 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     8101 ggcatggacg agctgtacaa gtaaccgcgg ccatgctaga gtccgcaaaa atcaccagtc
     8161 tctctctaca aatctatctc tctctatttt tctccagaat aatgtgtgag tagttcccag
     8221 ataagggaat tagggttctt atagggtttc gctcatgtgt tgagcatata agaaaccctt
     8281 agtatgtatt tgtatttgta aaatacttct atcaataaaa tttctaattc ctaaaaccaa
     8341 aatccagtga cctgcaggca tgcgacgtcg ggccctctag aggatccccg ggtaccgcga
     8401 attatcgatc atgagcggag aattaaggga gtcacgttat gacccccgcc gatgacgcgg
     8461 gacaagccgt tttacgtttg gaactgacag aaccgcaacg ttgaaggagc cactgagccg
     8521 cgggtttctg gagtttaatg agctaagcac atacgtcaga aaccattatt gcgcgttcaa
     8581 aagtcgccta aggtcactat cagctagcaa atatttcttg tcaaaaatgc tccactgacg
     8641 ttccataaat tcccctcggt atccaattag agtctcatat tcactctcaa ctcgatcgag
     8701 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     8761 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     8821 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     8881 tccaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     8941 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     9001 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     9061 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     9121 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     9181 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cggatgcccg
     9241 acggcgagga tctcgtcgtg acccacggcg atgcctgctt gccgaatatc atggtggaaa
     9301 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     9361 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
     9421 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
     9481 ttgacgagtt cttctgagcg ggactctggg gttcggactc tagctagagt caagcagatc
     9541 gttcaaacat ttggcaataa agtttcttaa gattgaatcc tgttgccggt cttgcgatga
     9601 ttatcatata atttctgttg aattacgtta agcatgtaat aattaacatg taatgcatga
     9661 cgttatttat gagatgggtt tttatgatta gagtcccgca attatacatt taatacgcga
     9721 tagaaaacaa aatatagcgc gcaaactagg ataaattatc gcgcgcggtg tcatctatgt
     9781 tactagatcg accggcatgc aagctgataa ttcaattcgg cgttaattca gtacattaaa
     9841 aacgtccgca atgtgttatt aagttgtcta agcgtcaatt tgtttacacc acaatatatc
     9901 ctgccaccag ccagccaaca gctccccgac cggcagctcg gcacaaaatc accactcgat
     9961 acaggcagcc catcagtccg ggacggcgtc agcgggagag ccgttgtaag gcggcagact
    10021 ttgctcatgt taccgatgct attcggaaga acggcaacta agctgccggg tttgaaacac
    10081 ggatgatctc gcggagggta gcatgttgat tgtaacgatg acagagcgtt gctgcctgtg
    10141 atcaattcgg gcacgaaccc agtggacata agcctgttcg gttcgtaagc tgtaatgcaa
    10201 gtagcgtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg
    10261 cgcagtggcg gttttcatgg cttgttatga ctgttttttt ggggtacagt ctatgcctcg
    10321 ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa
    10381 cgatgttacg cagcagggca gtcgccctaa aacaaagtta aacatcatgg gggaagcggt
    10441 gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga
    10501 accgacgttg ctggccgtac atttgtacgg ctccgcagt
//
