LOCUS       sequence-403451-       10464 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Determine where transit peptide targets GFP in N. benthamiana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10464)
  AUTHORS   DeTar RA, Chustecki JM, Martinez-Hottovy A, Ceriotti LF, Broz AK, 
            Lou X, Sanchez-Puerta MV, Elowsky C, Christensen AC, Sloan DB
  TITLE     Photosynthetic demands on translational machinery drive retention of
            redundant tRNA metabolism in plant organelles.
  JOURNAL   Proc Natl Acad Sci U S A. 2024 Dec 24;121(52):e2421485121. doi: 
            10.1073/pnas.2421485121. Epub 2024 Dec 18.
  PUBMED    39693336
REFERENCE   2  (bases 1 to 10464)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2024-12-24"; volume: "121(52):e2421485121. 
            doi"; pages: " 10.1073/pnas.2421485121. Epub 2024 Dec 18"
COMMENT     Sequence Label: Shim_GlyRS_TP_GFP_pK7FWG2
FEATURES             Location/Qualifiers
     source          1..10464
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             198..992
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(252..271)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     862..881
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      1032..1284
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    1377..1401
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             1922..2713
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2959..3547
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3448..3467
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3701..3718
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3733..3873
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3799..3818)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3959..3981
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      4217..4411
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(4477..5550)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(5979..6608)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    7939..7963
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        8656..9000
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     8944..8964
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    9100..9124
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9332..9356)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(9364..9366,9367..9369,9370..10083)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(9364..9366,9367..9369,9370..10080)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9409..9430)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9670..9689)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     10017..10038
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        join(10449..10464,1..164)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
ORIGIN
        1 ggagccactg agccgcgggt ttctggagtt taatgagcta agcacatacg tcagaaacca
       61 ttattgcgcg ttcaaaagtc gcctaaggtc actatcagct agcaaatatt tcttgtcaaa
      121 aatgctccac tgacgttcca taaattcccc tcggtatcca attagagtct catattcact
      181 ctcaactcga tcgaggcatg attgaacaag atggattgca cgcaggttct ccggccgctt
      241 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
      301 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
      361 gtgccctgaa tgaactccaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg
      421 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
      481 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
      541 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
      601 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
      661 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
      721 aggcgcggat gcccgacggc gaggatctcg tcgtgaccca cggcgatgcc tgcttgccga
      781 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
      841 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
      901 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
      961 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg gactctagct
     1021 agagtcaagc agatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg
     1081 ccggtcttgc gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta
     1141 acatgtaatg catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat
     1201 acatttaata cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg
     1261 cggtgtcatc tatgttacta gatcgaccgg catgcaagct gataattcaa ttcggcgtta
     1321 attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt caatttgttt
     1381 acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca gctcggcaca
     1441 aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg gagagccgtt
     1501 gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc aactaagctg
     1561 ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa cgatgacaga
     1621 gcgttgctgc ctgtgatcaa ttcgggcacg aacccagtgg acataagcct gttcggttcg
     1681 taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct tgaccgaacg
     1741 cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttggggt
     1801 acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat
     1861 gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaaacat
     1921 catgggggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat
     1981 cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg
     2041 cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa ggcttgatga
     2101 aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag
     2161 cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg
     2221 ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg
     2281 tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga
     2341 acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca
     2401 ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc
     2461 tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg
     2521 caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta
     2581 tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag atcgcttggc
     2641 ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt
     2701 agtcggcaaa taatgtctag ctagaaattc gttcaagccg acgccgcttc gcggcgcggc
     2761 ttaactcaag cgttagatgc actaagcaca taattgctca cagccaaact atcaggtcaa
     2821 gtctgctttt attattttta agcgtgcata ataagcccta cacaaattgg gagatatatc
     2881 atgcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     2941 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     3001 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     3061 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     3121 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     3181 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     3241 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     3301 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     3361 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     3421 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     3481 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     3541 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     3601 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     3661 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     3721 agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     3781 catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact
     3841 ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac
     3901 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
     3961 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagggtgcc
     4021 ttgatgtggg cgccggcggt cgagtggcga cggcgcggct tgtccgcgcc ctggtagatt
     4081 gcctggccgt aggccagcca tttttgagcg gccagcggcc gcgataggcc gacgcgaagc
     4141 ggcggggcgt agggagcgca gcgaccgaag ggtaggcgct ttttgcagct cttcggctgt
     4201 gcgctggcca gacagttatg cacaggccag gcgggtttta agagttttaa taagttttaa
     4261 agagttttag gcggaaaaat cgcctttttt ctcttttata tcagtcactt acatgtgtga
     4321 ccggttccca atgtacggct ttgggttccc aatgtacggg ttccggttcc caatgtacgg
     4381 ctttgggttc ccaatgtacg tgctatccac aggaaagaga ccttttcgac ctttttcccc
     4441 tgctagggca atttgcccta gcatctgctc cgtacattag gaaccggcgg atgcttcgcc
     4501 ctcgatcagg ttgcggtagc gcatgactag gatcgggcca gcctgccccg cctcctcctt
     4561 caaatcgtac tccggcaggt catttgaccc gatcagcttg cgcacggtga aacagaactt
     4621 cttgaactct ccggcgctgc cactgcgttc gtagatcgtc ttgaacaacc atctggcttc
     4681 tgccttgcct gcggcgcggc gtgccaggcg gtagagaaaa cggccgatgc cgggatcgat
     4741 caaaaagtaa tcggggtgaa ccgtcagcac gtccgggttc ttgccttctg tgatctcgcg
     4801 gtacatccaa tcagctagct cgatctcgat gtactccggc cgcccggttt cgctctttac
     4861 gatcttgtag cggctaatca aggcttcacc ctcggatacc gtcaccaggc ggccgttctt
     4921 ggccttcttc gtacgctgca tggcaacgtg cgtggtgttt aaccgaatgc aggtttctac
     4981 caggtcgtct ttctgctttc cgccatcggc tcgccggcag aacttgagta cgtccgcaac
     5041 gtgtggacgg aacacgcggc cgggcttgtc tcccttccct tcccggtatc ggttcatgga
     5101 ttcggttaga tgggaaaccg ccatcagtac caggtcgtaa tcccacacac tggccatgcc
     5161 ggccggccct gcggaaacct ctacgtgccc gtctggaagc tcgtagcgga tcacctcgcc
     5221 agctcgtcgg tcacgcttcg acagacggaa aacggccacg tccatgatgc tgcgactatc
     5281 gcgggtgccc acgtcataga gcatcggaac gaaaaaatct ggttgctcgt cgcccttggg
     5341 cggcttccta atcgacggcg caccggctgc cggcggttgc cgggattctt tgcggattcg
     5401 atcagcggcc gcttgccacg attcaccggg gcgtgcttct gcctcgatgc gttgccgctg
     5461 ggcggcctgc gcggccttca acttctccac caggtcatca cccagcgccg cgccgatttg
     5521 taccgggccg gatggtttgc gaccgctcac gccgattcct cgggcttggg ggttccagtg
     5581 ccattgcagg gccggcagac aacccagccg cttacgcctg gccaaccgcc cgttcctcca
     5641 cacatggggc attccacggc gtcggtgcct ggttgttctt gattttccat gccgcctcct
     5701 ttagccgcta aaattcatct actcatttat tcatttgctc atttactctg gtagctgcgc
     5761 gatgtattca gatagcagct cggtaatggt cttgccttgg cgtaccgcgt acatcttcag
     5821 cttggtgtga tcctccgccg gcaactgaaa gttgacccgc ttcatggctg gcgtgtctgc
     5881 caggctggcc aacgttgcag ccttgctgct gcgtgcgctc ggacggccgg cacttagcgt
     5941 gtttgtgctt ttgctcattt tctctttacc tcattaactc aaatgagttt tgatttaatt
     6001 tcagcggcca gcgcctggac ctcgcgggca gcgtcgccct cgggttctga ttcaagaacg
     6061 gttgtgccgg cggcggcagt gcctgggtag ctcacgcgct gcgtgatacg ggactcaaga
     6121 atgggcagct cgtacccggc cagcgcctcg gcaacctcac cgccgatgcg cgtgcctttg
     6181 atcgcccgcg acacgacaaa ggccgcttgt agccttccat ccgtgacctc aatgcgctgc
     6241 ttaaccagct ccaccaggtc ggcggtggcc catatgtcgt aagggcttgg ctgcaccgga
     6301 atcagcacga agtcggctgc cttgatcgcg gacacagcca agtccgccgc ctggggcgct
     6361 ccgtcgatca ctacgaagtc gcgccggccg atggccttca cgtcgcggtc aatcgtcggg
     6421 cggtcgatgc cgacaacggt tagcggttga tcttcccgca cggccgccca atcgcgggca
     6481 ctgccctggg gatcggaatc gactaacaga acatcggccc cggcgagttg cagggcgcgg
     6541 gctagatggg ttgcgatggt cgtcttgcct gacccgcctt tctggttaag tacagcgata
     6601 accttcatgc gttccccttg cgtatttgtt tatttactca tcgcatcata tacgcagcga
     6661 ccgcatgacg caagctgttt tactcaaata cacatcacct ttttagacgg cggcgctcgg
     6721 tttcttcagc ggccaagctg gccggccagg ccgccagctt ggcatcagac aaaccggcca
     6781 ggatttcatg cagccgcacg gttgagacgt gcgcgggcgg ctcgaacacg tacccggccg
     6841 cgatcatctc cgcctcgatc tcttcggtaa tgaaaaacgg ttcgtcctgg ccgtcctggt
     6901 gcggtttcat gcttgttcct cttggcgttc attctcggcg gccgccaggg cgtcggcctc
     6961 ggtcaatgcg tcctcacgga aggcaccgcg ccgcctggcc tcggtgggcg tcacttcctc
     7021 gctgcgctca agtgcgcggt acagggtcga gcgatgcacg ccaagcagtg cagccgcctc
     7081 tttcacggtg cggccttcct ggtcgatcag ctcgcgggcg tgcgcgatct gtgccggggt
     7141 gagggtaggg cgggggccaa acttcacgcc tcgggccttg gcggcctcgc gcccgctccg
     7201 ggtgcggtcg atgattaggg aacgctcgaa ctcggcaatg ccggcgaaca cggtcaacac
     7261 catgcggccg gccggcgtgg tggtgtcggc ccacggctct gccaggctac gcaggcccgc
     7321 gccggcctcc tggatgcgct cggcaatgtc cagtaggtcg cgggtgctgc gggccaggcg
     7381 gtctagcctg gtcactgtca caacgtcgcc agggcgtagg tggtcaagca tcctggccag
     7441 ctccgggcgg tcgcgcctgg tgccggtgat cttctcggaa aacagcttgg tgcagccggc
     7501 cgcgtgcagt tcggcccgtt ggttggtcaa gtcctggtcg tcggtgctga cgcgggcata
     7561 gcccagcagg ccagcggcgg cgctcttgtt catggcgtaa tgtctccggt tctagtcgca
     7621 agtattctac tttatgcgac taaaacacgc gacaagaaaa cgccaggaaa agggcagggc
     7681 ggcagcctgt cgcgtaactt aggacttgtg cgacatgtcg ttttcagaag acggctgcac
     7741 tgaacgtcag aagccgactg cactatagca gcggaggggt tggatcaaag tactttaaag
     7801 tactttaaag tactttaaag tactttgatc ccgaggggaa ccctgtggtt ggcatgcaca
     7861 tacaaatgga cgaacggata aaccttttca cgccctttta aatatccgat tattctaata
     7921 aacgctcttt tctcttaggt ttacccgcca atatatcctg tcaaacactg atagtttaaa
     7981 ctgaaggcgg gaaacgacaa tctgatccaa gctcaagcta agcttgagct ctcccatatg
     8041 gtcgactaga gccaagctga tctcctttgc cccggagatc accatggacg actttctcta
     8101 tctctacgat ctaggaagaa agttcgacgg agaaggtgac gataccatgt tcaccaccga
     8161 taatgagaag attagcctct tcaatttcag aaagaatgct gacccacaga tggttagaga
     8221 ggcctacgcg gcaggtctca tcaagacgat ctacccgagt aataatctcc aggagatcaa
     8281 ataccttccc aagaaggtta aagatgcagt caaaagattc aggactaact gcatcaagaa
     8341 cacagagaaa gatatatttc tcaagatcag aagtactatt ccagtatgga cgattcaagg
     8401 cttgcttcat aaaccaaggc aagtaataga gattggagtc tctaagaaag tagttcctac
     8461 tgaatcaaag gccatggagt caaaaattca gatcgaggat ctaacagaac tcgccgtgaa
     8521 gactggcgaa cagttcatac agagtctttt acgactcaat gacaagaaga aaatcttcgt
     8581 caacatggtg gagcacgaca ctctcgtcta ctccaagaat atcaaagata cagtctcaga
     8641 agaccaaagg gctattgaga cttttcaaca aagggtaata tcgggaaacc tcctcggatt
     8701 ccattgccca gctatctgtc acttcatcaa aaggacagta gaaaaggaag gtggcaccta
     8761 caaatgccat cattgcgata aaggaaaggc tatcgttcaa gatgcccctg ccgacagtgg
     8821 tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac
     8881 gtcttcaaag caagtggatt gatgtgatat ctccactgac gtaagggatg acgcacaatc
     8941 ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagaggac
     9001 tccggtattt ttacaacaat taccacaaca aaacaaacaa caaacaacat tacaatttac
     9061 tattctagtc gacctgcagg cggccgcact agtgatatca caagtttgta caaaaaagca
     9121 ggctccgcgg ccgccccctt caccatgaga tttcatgctc ttccagttgc tcttcttctt
     9181 tctcatccac ttagatctct tggatctctt ggacatagac atcatcaaaa ggctagacca
     9241 ccatctgctt cttttcttct tagaagaaga tattcttttg ctatggttgc tgatgatgat
     9301 tattttagaa gagctaaggg tgggcgcgcc gacccagctt tcttgtacaa agtggtgata
     9361 tcaatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
     9421 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
     9481 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     9541 accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg
     9601 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
     9661 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     9721 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     9781 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag
     9841 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc
     9901 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     9961 cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg
    10021 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
    10081 taaccgcggc catgctagag tccgcaaaaa tcaccagtct ctctctacaa atctatctct
    10141 ctctattttt ctccagaata atgtgtgagt agttcccaga taagggaatt agggttctta
    10201 tagggtttcg ctcatgtgtt gagcatataa gaaaccctta gtatgtattt gtatttgtaa
    10261 aatacttcta tcaataaaat ttctaattcc taaaaccaaa atccagtgac ctgcaggcat
    10321 gcgacgtcgg gccctctaga ggatccccgg gtaccgcgaa ttatcgatca tgagcggaga
    10381 attaagggag tcacgttatg acccccgccg atgacgcggg acaagccgtt ttacgtttgg
    10441 aactgacaga accgcaacgt tgaa
//
