LOCUS       sequence-403469-       10264 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  S. cerevisiae expression vector for GtFz1 native guide.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10264)
  AUTHORS   Saito M, Xu P, Faure G, Maguire S, Kannan S, Altae-Tran H, Vo S, 
            Desimone A, Macrae RK, Zhang F
  TITLE     Fanzor is a eukaryotic programmable RNA-guided endonuclease.
  JOURNAL   Nature. 2023 Jun 28. doi: 10.1038/s41586-023-06356-2.
  PUBMED    37380027
REFERENCE   2  (bases 1 to 10264)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2023 Jun 28. doi: 10.1038/s41586-023-06356-2."
COMMENT     Sequence Label: pMJ235: pRS426-His-MBP-GtFz1_Native
FEATURES             Location/Qualifiers
     source          1..10264
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      69..1411
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    401..448
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        1438..1542
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1543..1611,1612..2403)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1761..1780)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2574..3162
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3063..3082
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3316..3333
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3450..3471
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3486..3491,3492..3509,3510..3516)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3524..3540
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3529..3551
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3548..3564
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3548..3564
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3583..3603
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3585..3603
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    3666..3690
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    3821..3938
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     4119..4142
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     CDS             4260..4289
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             4299..5399
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     5373..5396
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             5454..5474
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     protein_bind    complement(8208..8232)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(8280..8299)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8281..8299)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8309..8326)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8309..8325)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8318..8340)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8466..8921
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8553..8572)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8763..8784
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(9052..9855)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(9856..10071)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(10095..10114)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10214..10236
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagtatga tccaatatca aaggaaatga tagcattgaa ggatgagact aatccaattg
      121 aggagtggca gcatatagaa cagctaaagg gtagtgctga aggaagcata cgataccccg
      181 catggaatgg gataatatca caggaggtac tagactacct ttcatcctac ataaatagac
      241 gcatataagt acgcatttaa gcataaacac gcactatgcc gttcttctca tgtatatata
      301 tatacaggca acacgcagat ataggtgcga cgtgaacagt gagctgtatg tgcgcagctc
      361 gcgttgcatt ttcggaagcg ctcgttttcg gaaacgcttt gaagttccta ttccgaagtt
      421 cctattctct agaaagtata ggaacttcag agcgcttttg aaaaccaaaa gcgctctgaa
      481 gacgcacttt caaaaaacca aaaacgcacc ggactgtaac gagctactaa aatattgcga
      541 ataccgcttc cacaaacatt gctcaaaagt atctctttgc tatatatctc tgtgctatat
      601 ccctatataa cctacccatc cacctttcgc tccttgaact tgcatctaaa ctcgacctct
      661 acatttttta tgtttatctc tagtattact ctttagacaa aaaaattgta gtaagaacta
      721 ttcatagagt gaatcgaaaa caatacgaaa atgtaaacat ttcctatacg tagtatatag
      781 agacaaaata gaagaaaccg ttcataattt tctgaccaat gaagaatcat caacgctatc
      841 actttctgtt cacaaagtat gcgcaatcca catcggtata gaatataatc ggggatgcct
      901 ttatcttgaa aaaatgcacc cgcagcttcg ctagtaatca gtaaacgcgg gaagtggagt
      961 caggcttttt ttatggaaga gaaaatagac accaaagtag ccttcttcta accttaacgg
     1021 acctacagtg caaaaagtta tcaagagact gcattataga gcgcacaaag gagaaaaaaa
     1081 gtaatctaag atgctttgtt agaaaaatag cgctctcggg atgcattttt gtagaacaaa
     1141 aaagaagtat agattctttg ttggtaaaat agcgctctcg cgttgcattt ctgttctgta
     1201 aaaatgcagc tcagattctt tgtttgaaaa attagcgctc tcgcgttgca tttttgtttt
     1261 acaaaaatga agcacagatt cttcgttggt aaaatagcgc tttcgcgttg catttctgtt
     1321 ctgtaaaaat gcagctcaga ttctttgttt gaaaaattag cgctctcgcg ttgcattttt
     1381 gttctacaaa atgaagcaca gatgcttcgt tcaggtggca cttttcgggg aaatgtgcgc
     1441 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     1501 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     1561 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     1621 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     1681 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     1741 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     1801 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     1861 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     1921 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     1981 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     2041 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     2101 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     2161 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     2221 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     2281 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     2341 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     2401 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     2461 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     2521 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     2581 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     2641 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     2701 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     2761 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     2821 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     2881 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     2941 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     3001 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     3061 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     3121 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     3181 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     3241 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     3301 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     3361 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     3421 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     3481 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     3541 tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa
     3601 agggaacaaa agctggagct ccaccgcggt ggcggccccc gtcccaccat cgggcgcgga
     3661 tcatcacaag tttgtacaaa aaagcaggct ctttggccat ataggccacc aggtcctgca
     3721 ggcgcgctct tcaggaggcg cgccaactgc cctcttaatt aacgaagaag atcggaccgg
     3781 ggccgcggat ctttgcaaag atccaaaaaa agcatgcgta cggattagaa gccgccgagc
     3841 gggtgacagc cctccgaagg aagactctcc tccgtgcgtc ctcgtcttca ccggtcgcgt
     3901 tcctgaaacg cagatgtgcc tcgcgccgca ctgctccgaa caataaagat tctacaatac
     3961 tagcttttat ggttatgaag aggaaaattg gcagtaacct ggccccacaa acctcaaatg
     4021 aacgaatcaa attaacaacc agatatctcg actgaaaaaa aaggtttaaa ccagttccct
     4081 gaaattattc ccctacttga ctaataagta tataaagacg gtaggtattg attgtaattc
     4141 tgtaaatcta tttcttaaac ttcttgaatt ctacttttat agttagtctt ttttttagtt
     4201 ttaaaacacc aagaacttag tttcgaataa acacacataa ataaaccatg aaatcttctc
     4261 accatcacca tcaccatcac catcaccatg gttcttctat gaaaatcgaa gaaggtaaac
     4321 tggtaatctg gattaacggc gataaaggct ataacggtct cgctgaagtc ggtaagaaat
     4381 tcgagaaaga taccggaatt aaagtcaccg ttgagcatcc ggataaactg gaagagaaat
     4441 tcccacaggt tgcggcaact ggcgatggcc ctgacattat cttctgggca cacgaccgct
     4501 ttggtggcta cgctcaatct ggcctgttgg ctgaaatcac cccggacaaa gcgttccagg
     4561 acaagctgta tccgtttacc tgggatgccg tacgttacaa cggcaagctg attgcttacc
     4621 cgatcgctgt tgaagcgtta tcgctgattt ataacaaaga tctgctgccg aacccgccaa
     4681 aaacctggga agagatcccg gcgctggata aagaactgaa agcgaaaggt aagagcgcgc
     4741 tgatgttcaa cctgcaagaa ccgtacttca cctggccgct gattgctgct gacgggggtt
     4801 atgcgttcaa gtatgaaaac ggcaagtacg acattaaaga cgtgggcgtg gataacgctg
     4861 gcgcgaaagc gggtctgacc ttcctggttg acctgattaa aaacaaacac atgaatgcag
     4921 acaccgatta ctccatcgca gaagctgcct ttaataaagg cgaaacagcg atgaccatca
     4981 acggcccgtg ggcatggtcc aacatcgaca ccagcaaagt gaattatggt gtaacggtac
     5041 tgccgacctt caagggtcaa ccatccaaac cgttcgttgg cgtgctgagc gcaggtatta
     5101 acgccgccag tccgaacaaa gagctggcaa aagagttcct cgaaaactat ctgctgactg
     5161 atgaaggtct ggaagcggtt aataaagaca aaccgctggg tgccgtagcg ctgaagtctt
     5221 acgaggaaga gttggcgaaa gatccacgta ttgccgccac tatggaaaac gcccagaaag
     5281 gtgaaatcat gccgaacatc ccgcagatgt ccgctttctg gtatgccgtg cgtactgcgg
     5341 tgatcaacgc cgccagcggt cgtcagactg tcgatgaagc cctgaaagac gcgcagacta
     5401 attcgagctc gaacaacaac aacaataaca ataacaacaa cctcgggatc gaggaaaacc
     5461 tgtacttcca atccaatgca tcaaacatta ggattgtaaa acgaaaggcg aagggcttct
     5521 tcaagtgtga ggacctagtc acaatcaagg atgctgtcaa ggcagctcat aggatcatgt
     5581 cagatgcgtc catactcgtc cggtcatatt atctgcgatg gttccagagc tcatatcccc
     5641 ttgattcaga tgacaaggaa cttgagcttg agcacttcca tatatccatg gcctgcagca
     5701 tagtccaggg tataacaagg cctccagtcc gtggtgttgg tcctgaacaa tctgtcaaga
     5761 tagacgtctt caatgacatg ttagatgagt ataagaggct gtacgagcgt gctccaaatg
     5821 ataaagagaa cgaaacggac ctctctctat cgcatgtctt agcatattcc atcgacaacc
     5881 ttcttaccgc gtataaaaac aacatcgaag cccatttctc aaaatatgtc aaacgcttca
     5941 tccgatgtga tatgttggct aagggcttca ataagtctga ggcaaacaga gtggcggcaa
     6001 tatataccaa tgcctatatt tatgactcat ccctcgacct ggaaccagac tttatggaaa
     6061 gattaggcct tgaggcaaca agttattcat ctctattccc ctctaagata aataagggcg
     6121 gttttccaag ggtctatgac ctgaaggcca acccttgggt ctatcttcca aagatggtca
     6181 tgataaatca ggccttagag acagacttct cttctgtaga gcataaggag aggaggctgc
     6241 tcaatccact gcccttctat tcatcgtttg ttccgatgca catacggatt gatacatccg
     6301 gcttatcgca gctgctgatg actaaggata ggcttgatga tttcaagaga tcttatcttg
     6361 ctgagtttgg agtaagtcta aatataaaga acaagggtga tatgctagca agttttgaga
     6421 agatctttgg ccggaaggca acatccaata gagaggccgg attgtatgca acagagatgt
     6481 ggagcttctt gacaaacctg aagacatgcc gtcaatggaa ggagcttgat ggggttgttc
     6541 gtaagaacga tcccaaggga acacaatgga tgtttgacaa tgctgttgta acggacggtg
     6601 tttcaatctc attccaggtg atcgataaca gcatgtttgg acggaaggcc ttctctggca
     6661 ggaagaagag ggtagcatgt caggaggcca atgatgaaga agatagcaag caggtaacaa
     6721 gagaagagct aaagacatcg aagctcttgg gatgtgaccc tgggaagaga gacatcctag
     6781 caataacaga tggcataaag actatctgtt acaccaaggg gcagagagac atggacacgc
     6841 ataagacaat aaggttgaga accagtctga agaggcgccg aggctgcggc ctagaggagt
     6901 atgagacaca ggtgatgaac cggttccaga aacggtcatg ccatcctgag atgtttcgac
     6961 gctatgcctg ttccaggaag aggatggaac acatgctgct tgaatgttat tctcatccag
     7021 tctttagaga gttcaaattc ttggtttaca acaagactaa gagctccgaa cataggttca
     7081 tgcatagggt gttggaaaca ttcaaacggc cgcaaacaaa cctgagcaag gcgaggtgtg
     7141 cgtcaggagt tatgcgtatg aatgccttga aggaggtcca aaggcatgga gatatcatta
     7201 tagggtgggg caactgggga aagaatccaa acgcgctacg atgctcggca ggaccaaccc
     7261 caggcattgg aataaggaga aggtttgaat cgctgttcaa aacaacaaca gtgccagaac
     7321 actacacctc ccaagaatgt ccgagttgta agggacgatg cctaagaaag gcaactggca
     7381 acccgatcat gagacaccat ctgcttcgtt gtacaaacga tagttgctgt agcaggtggt
     7441 ggaatcggaa tgtagccggg gcgttcaaca tcctaacaag gctgttggat ggacaaacac
     7501 tatccggtaa cgaaactacc ggagacgggt taggaggtga cgacctctaa aacctagaac
     7561 ttagagtgca aaaacgccat tacgattgtg atgcctattc aagggtgtcc caagtgtaaa
     7621 aagaaagcac tctaagagca ttaaactcta ctctgtcaat gttctcaata aggatgtctg
     7681 aagcgttctc attgcatgat agattgttcc aagccttgtc ggtcatctca tcgattttta
     7741 tatttatgat tttcatagca gtcggattat tattcaaaca aagaccttct atgtcaagtt
     7801 tgctctcatc aacccatggt aatagtctat aagacatatc ttatcctcct taagtatttc
     7861 ctgttgtgct taagtcgact ttgttcccac tgtactttta gctcgtacaa aatacaatat
     7921 acttttcatt tctccgtaaa caacatgttt tcccatgtaa tatccttttc tatttttcgt
     7981 tccgttacca actttacaca tactttatat agctattcac ttctatacac taaaaaacta
     8041 agacaatttt aattttgctg cctgccatat ttcaatttgt tataaattcc tataatttat
     8101 cctattagta gctaaaaaaa gatgaatgtg aatcgaatcc taagagaatt cccctcgaag
     8161 atcgatctgg cggcccggcc ggccgcggcc gcactcgaga tatctagacc cagctttctt
     8221 gtacaaagtg gtgatagctt gtcgagaagt actcgagggg gggcccggta cccaattcgc
     8281 cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg
     8341 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     8401 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     8461 aatggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     8521 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     8581 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     8641 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     8701 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     8761 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     8821 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     8881 atttaacgcg aattttaaca aaatattaac gcttacaatt tcctgatgcg gtattttctc
     8941 cttacgcatc tgtgcggtat ttcacaccgc atagggtaat aactgatata attaaattga
     9001 agctctaatt tgtgagttta gtatacatgc atttacttat aatacagttt tttagttttg
     9061 ctggccgcat cttctcaaat atgcttccca gcctgctttt ctgtaacgtt caccctctac
     9121 cttagcatcc cttccctttg caaatagtcc tcttccaaca ataataatgt cagatcctgt
     9181 agagaccaca tcatccacgg ttctatactg ttgacccaat gcgtctccct tgtcatctaa
     9241 acccacaccg ggtgtcataa tcaaccaatc gtaaccttca tctcttccac ccatgtctct
     9301 ttgagcaata aagccgataa caaaatcttt gtcgctcttc gcaatgtcaa cagtaccctt
     9361 agtatattct ccagtagata gggagccctt gcatgacaat tctgctaaca tcaaaaggcc
     9421 tctaggttcc tttgttactt cttctgccgc ctgcttcaaa ccgctaacaa tacctgggcc
     9481 caccacaccg tgtgcattcg taatgtctgc ccattctgct attctgtata cacccgcaga
     9541 gtactgcaat ttgactgtat taccaatgtc agcaaatttt ctgtcttcga agagtaaaaa
     9601 attgtacttg gcggataatg cctttagcgg cttaactgtg ccctccatgg aaaaatcagt
     9661 caagatatcc acatgtgttt ttagtaaaca aattttggga cctaatgctt caactaactc
     9721 cagtaattcc ttggtggtac gaacatccaa tgaagcacac aagtttgttt gcttttcgtg
     9781 catgatatta aatagcttgg cagcaacagg actaggatga gtagcagcac gttccttata
     9841 tgtagctttc gacatgattt atcttcgttt cctgcaggtt tttgttctgt gcagttgggt
     9901 taagaatact gggcaatttc atgtttcttc aacactacat atgcgtatat ataccaatct
     9961 aagtctgtgc tccttccttc gttcttcctt ctgttcggag attaccgaat caaaaaaatt
    10021 tcaaggaaac cgaaatcaaa aaaaagaata aaaaaaaaat gatgaattga aaaggtggta
    10081 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg
    10141 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
    10201 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
    10261 gcga
//
