LOCUS       sequence-403577-       12691 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Over-expression of 3xFLAG-tagged human LUC7L2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12691)
  AUTHORS   Jourdain AA, Begg BE, Mick E, Shah H, Calvo SE, Skinner OS, Sharma 
            R, Blue SM, Yeo GW, Burge CB, Mootha VK
  TITLE     Loss of LUC7L2 and U1 snRNP subunits shifts energy metabolism from 
            glycolysis to OXPHOS.
  JOURNAL   Mol Cell. 2021 May 6;81(9):1905-1919.e12. doi: 
            10.1016/j.molcel.2021.02.033. Epub 2021 Apr 13.
  PUBMED    33852893
REFERENCE   2  (bases 1 to 12691)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2021.02.033"; journalName: "Mol Cell"; date: 
            "2021-05-6- 6"; volume: "81"; issue: "9"; pages: "1905-1919.e12"
COMMENT     Sequence Label: pWPI-LUC7L2-3xFLAG
FEATURES             Location/Qualifiers
     source          1..12691
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             2..635
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    682..807
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1300..1533
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1718..1762
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2145..3323
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2376..3314
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3271..3291
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    3371..3488
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             4711..4776
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    4840..5391
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4984..5001)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5211..5230
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5394..6188
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5448..5467)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6058..6077
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6362..6483
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    6522..7110
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6575..6595)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6993..7004)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(7254..7287)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             7307..7487
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(7512..7530)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7513..7530)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7820..7839)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(8053..8072)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8172..8194
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8232..8250)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8318..8422
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8423..8491,8492..9283)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8641..8660)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9454..10042
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9943..9962
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10196..10213
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10283..10303)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10288..10617
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10468..10603
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          11751..11816
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             11946..11966
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    12391..12525
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac
       61 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
      121 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
      181 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
      241 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
      301 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
      361 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
      421 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
      481 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
      541 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
      601 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
      661 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
      721 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
      781 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
      841 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
      901 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
      961 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     1021 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     1081 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     1141 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1201 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1261 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1321 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     1381 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1441 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1501 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1561 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     1621 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     1681 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     1741 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     1801 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     1861 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     1921 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     1981 agacagatcc attcgattag tgaacggatc tcgacggtat cgatcacgag actagcctcg
     2041 accatcgatg tcgacgataa gctttgcaaa gatggataaa gttttaaaca gagaggaatc
     2101 tttgcagcta atggaccttc taggtcttga aaggagtggg aattggctcc ggtgcccgtc
     2161 agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt
     2221 gaaccggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc
     2281 tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg
     2341 ttctttttcg caacgggttt gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg
     2401 ggcctggcct ctttacgggt tatggccctt gcgtgccttg aattacttcc acctggctgc
     2461 agtacgtgat tcttgatccc gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt
     2521 gcgcttaagg agccccttcg cctcgtgctt gagttgaggc ctggcctggg cgctggggcc
     2581 gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc tgctttcgat aagtctctag
     2641 ccatttaaaa tttttgatga cctgctgcga cgcttttttt ctggcaagat agtcttgtaa
     2701 atgcgggcca agatctgcac actggtattt cggtttttgg ggccgcgggc ggcgacgggg
     2761 cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa
     2821 tcggacgggg gtagtctcaa gctggccggc ctgctctggt gcctggcctc gcgccgccgt
     2881 gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc accagttgcg tgagcggaaa
     2941 gatggccgct tcccggccct gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag
     3001 agcgggcggg tgagtcaccc acacaaagga aaagggcctt tccgtcctca gccgtcgctt
     3061 catgtgactc cacggagtac cgggcgccgt ccaggcacct cgattagttc tcgagctttt
     3121 ggagtacgtc gtctttaggt tggggggagg ggttttatgc gatggagttt ccccacactg
     3181 agtgggtgga gactgaagtt aggccagctt ggcacttgat gtaattctcc ttggaatttg
     3241 ccctttttga gtttggatct tggttcattc tcaagcctca gacagtggtt caaagttttt
     3301 ttcttccatt tcaggtgtcg tgaggaattt cgacatttaa atttaattaa tctcgacggt
     3361 atcggttaac ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
     3421 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
     3481 aaaattttat cgatcacgag actagcctcg aggtttcata tgtacagagg atccatgagc
     3541 gcccaggccc agatgagggc catgctggac cagctgatgg gcaccagcag ggacggcgac
     3601 accaccaggc agaggatcaa gttcagcgac gacagggtgt gcaagagcca cctgctgaac
     3661 tgctgccccc acgacgtgct gagcggcacc aggatggacc tgggcgagtg cctgaaggtg
     3721 cacgacctgg ccctgagggc cgactacgag atcgccagca aggagcagga cttcttcttc
     3781 gagctggacg ccatggacca cctgcagagc ttcatcgccg actgcgacag gaggaccgag
     3841 gtggccaaga agaggctggc cgagacccag gaggagatca gcgccgaggt ggccgccaag
     3901 gccgagaggg tgcacgagct gaacgaggag atcggcaagc tgctggccaa ggtggagcag
     3961 ctgggcgccg agggcaacgt ggaggagagc cagaaggtga tggacgaggt ggagaaggcc
     4021 agggccaaga agagggaggc cgaggaggtg tacaggaaca gcatgcccgc cagcagcttc
     4081 cagcagcaga agctgagggt gtgcgaggtg tgcagcgcct acctgggcct gcacgacaac
     4141 gacaggaggc tggccgacca cttcggcggc aagctgcacc tgggcttcat cgagatcagg
     4201 gagaagctgg aggagctgaa gagggtggtg gccgagaagc aggagaagag gaaccaggag
     4261 aggctgaaga ggagggagga gagggagagg gaggagaggg agaagctgag gaggagcagg
     4321 agccacagca agaaccccaa gaggagcagg agcagggagc acaggaggca caggagcagg
     4381 agcatgagca gggagaggaa gaggaggacc aggagcaaga gcagggagaa gaggcacagg
     4441 cacaggagca ggagcagcag caggagcagg agcaggagcc accagaggag caggcacagc
     4501 agcagggaca ggagcaggga gaggagcaag aggaggagca gcaaggagag gttcagggac
     4561 caggacctgg ccagctgcga cagggacagg agcagcaggg acaggagccc cagggacagg
     4621 gacaggaagg acaagaagag gagctacgag agcgccaacg gcaggagcga ggacaggagg
     4681 agcagcgagg agagggaggc cggcgagatc gattataaag atcatgatgg cgattataaa
     4741 gatcatgata ttgattataa agatgatgat gataaataat agtgagtcga cactagtccc
     4801 gggactacgg gctgcaggaa ttccgccccn ccccccctaa cgttactggc cgaagccgct
     4861 tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg
     4921 gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt
     4981 cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg
     5041 aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac
     5101 ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg
     5161 cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct
     5221 caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg
     5281 atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aacgtctagg
     5341 ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataat accatgattg
     5401 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
     5461 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
     5521 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg
     5581 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
     5641 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
     5701 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
     5761 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
     5821 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
     5881 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg
     5941 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
     6001 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
     6061 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
     6121 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
     6181 tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc
     6241 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
     6301 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
     6361 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     6421 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     6481 ttatcatgtc tgtataccgt cgacctcgag ggaattccga taatcaacct ctggattaca
     6541 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     6601 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     6661 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     6721 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     6781 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     6841 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     6901 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga
     6961 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     7021 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     7081 gtcggatctc cctttgggcc gcctccccgc atcgggaatt cgagctcggt acctttaaga
     7141 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     7201 gaagggctaa ttcactccca acgaagacaa gatgggatca attcaccatg ggaataactt
     7261 cgtatagcat acattatacg aagttatgct gctttttgct tgtactgggt ctctctggtt
     7321 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     7381 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     7441 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagca tctagaatta
     7501 attccgtgta ttctatagtg tcacctaaat cgtatgtgta tgatacataa ggttatgtat
     7561 taattgtagc cgcgttctaa cgacaatatg tacaagccta attgtgtagc atctggctta
     7621 ctgaagcaga ccctatcatc tctctcgtaa actgccgtca gagtcggttt ggttggacga
     7681 accttctgag tttctggtaa cgccgtcccg cacccggaaa tggtcagcga accaatcagc
     7741 agggtcatcg ctagccagat cctctacgcc ggacgcatcg tggccggcat caccggcgcc
     7801 acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg atggggaaga tcgggctcgc
     7861 cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg
     7921 ggactgttgg gcgccatctc cttgcatgca ccattccttg cggcggcggt gctcaacggc
     7981 ctcaacctac tactgggctg cttcctaatg caggagtcgc ataagggaga gcgtcgaatg
     8041 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc
     8101 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     8161 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     8221 gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     8281 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     8341 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     8401 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     8461 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     8521 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     8581 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     8641 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     8701 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     8761 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     8821 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     8881 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     8941 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     9001 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     9061 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     9121 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     9181 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     9241 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     9301 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     9361 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     9421 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     9481 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     9541 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     9601 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     9661 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     9721 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     9781 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     9841 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     9901 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     9961 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
    10021 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
    10081 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
    10141 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
    10201 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
    10261 gccgattcat taatgcagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
    10321 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
    10381 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
    10441 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
    10501 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
    10561 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
    10621 cttggacaca agacaggctt gcgagatatg tttgagaata ccactttatc ccgcgtcagg
    10681 gagaggcagt gcgtaaaaag acgcggactc atgtgaaata ctggttttta gtgcgccaga
    10741 tctctataat ctcgcgcaac ctattttccc ctcgaacact ttttaagccg tagataaaca
    10801 ggctgggaca cttcacatga gcgaaaaata catcgtcacc tgggacatgt tgcagatcca
    10861 tgcacgtaaa ctcgcaagcc gactgatgcc ttctgaacaa tggaaaggca ttattgccgt
    10921 aagccgtggc ggtctgtacc gggtgcgtta ctggcgcgtg aactgggtat tcgtcatgtc
    10981 gataccgttt gtatttccag ctacgatcac gacaaccagc gcgagcttaa agtgctgaaa
    11041 cgcgcagaag gcgatggcga aggcttcatc gttattgatg acctggtgga taccggtggt
    11101 actgcggttg cgattcgtga aatgtatcca aaagcgcact ttgtcaccat cttcgcaaaa
    11161 ccggctggtc gtccgctggt tgatgactat gttgttgata tcccgcaaga tacctggatt
    11221 gaacagccgt gggatatggg cgtcgtattc gtcccgccaa tctccggtcg ctaatctttt
    11281 caacgcctgg cactgccggg cgttgttctt tttaacttca ggcgggttac aatagtttcc
    11341 agtaagtatt ctggaggctg catccatgac acaggcaaac ctgagcgaaa ccctgttcaa
    11401 accccgcttt aaacatcctg aaacctcgac gctagtccgc cgctttaatc acggcgcaca
    11461 accgcctgtg cagtcggccc ttgatggtaa aaccatccct cactggtatc gcatgattaa
    11521 ccgtctgatg tggatctggc gcggcattga cccacgcgaa atcctcgacg tccaggcacg
    11581 tattgtgatg agcgatgccg aacgtaccga cgatgattta tacgatacgg tgattggcta
    11641 ccgtggcggc aactggattt atgagtgggc cccggatctt tgtgaaggaa ccttacttct
    11701 gtggtgtgac ataattggac aaactaccta cagagattta aagctctaag gtaaatataa
    11761 aatttttaag tgtataatgt gttaaactac tgattctaat tgtttgtgta ttttagattc
    11821 caacctatgg aactgatgaa tgggagcagt ggtggaatgc ctttaatgag gaaaacctgt
    11881 tttgctcaga agaaatgcca tctagtgatg atgaggctac tgctgactct caacattcta
    11941 ctcctccaaa aaagaagaga aaggtagaag accccaagga ctttccttca gaattgctaa
    12001 gttttttgag tcatgctgtg tttagtaata gaactcttgc ttgctttgct atttacacca
    12061 caaaggaaaa agctgcactg ctatacaaga aaattatgga aaaatattct gtaaccttta
    12121 taagtaggca taacagttat aatcataaca tactgttttt tcttactcca cacaggcata
    12181 gagtgtctgc tattaataac tatgctcaaa aattgtgtac ctttagcttt ttaatttgta
    12241 aaggggttaa taaggaatat ttgatgtata gtgccttgac tagagatcat aatcagccat
    12301 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg
    12361 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac
    12421 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
    12481 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa
    12541 gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc caattacctg
    12601 tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt
    12661 atttgttaaa tatgtactac aaacttagta g
//
