LOCUS       sequence-403584-       11022 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  2nd generation lentiviral transfer plasmid for engineering cells to 
            express human CD20.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11022)
  AUTHORS   Hamilton JR, Chen E, Perez BS, Sandoval Espinoza CR, Kang MH, 
            Trinidad M, Ngo W, Doudna JA
  TITLE     In vivo human T cell engineering with enveloped delivery vehicles.
  JOURNAL   Nat Biotechnol. 2024 Jan 11. doi: 10.1038/s41587-023-02085-z.
  PUBMED    38212493
REFERENCE   2  (bases 1 to 11022)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2024 Jan 11. doi: 10.1038/s41587-023-02085-z."
COMMENT     Sequence Label: pJRH-1328 LV EF1a-CD20 IRES-EGFP
FEATURES             Location/Qualifiers
     source          1..11022
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(75..94)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        260..639
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        641..839
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     794..814
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             857..1037
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1084..1209
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1702..1935
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2120..2164
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2462..2579
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2689..3866
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2920..3857
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3814..3834
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    4821..5394
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4844..5396
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4988..5005)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5215..5234
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(5408..5410,5411..5413,5414..6127)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5408..5410,5411..5413,5414..6124)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5453..5474)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5714..5733)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6061..6082
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6142..6730
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6195..6215)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6613..6624)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6802..7035
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7061..7078)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7067..7291
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7337..7765
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7424..7443)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7634..7655
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7774..7794)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7779..8108
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7959..8094
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8021..8040
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8156..8203
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8222..8596
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8726..8847
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8763..8782)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8817..8836
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8896..8912)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8896..8912)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8909..8931)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8920..8936
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8944..8950,8951..8968,8969..8974))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8989..9010
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9127..9144)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9298..9886)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9378..9397)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10057..10848,10849..10917))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10680..10699
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10918..11022)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc cgatccccta
       61 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct
      121 gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa
      181 ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc
      241 gatgtacggg ccagatattg acattgatta ttgactagtt attaatagta atcaattacg
      301 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
      361 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
      421 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
      481 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
      541 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
      601 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
      661 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
      721 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
      781 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcagc
      841 gcgttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
      901 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
      961 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     1021 gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag
     1081 gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg
     1141 cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc
     1201 gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg
     1261 ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa
     1321 cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga
     1381 cagctacaac catcccttca gacaggatca gaagaactta gatcattata taatacagta
     1441 gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac
     1501 aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt
     1561 cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt
     1621 agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag
     1681 agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag
     1741 cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat
     1801 agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact
     1861 cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa
     1921 ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt
     1981 gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg
     2041 gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga
     2101 atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag
     2161 tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat
     2221 agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt
     2281 taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga
     2341 caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt
     2401 agtgaacgga tcggcactgc gtgcgccaat tctgcagaca aatggcagta ttcatccaca
     2461 attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
     2521 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc
     2581 gggtttatta cagggacagc agagatccag tttggttaat taaggtacca tcgatcttaa
     2641 gtcgcgactc gagaattcgc tagctaggtc ttgaaaggag tgggaattgg ctccggtgcc
     2701 cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc
     2761 aattgatccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac
     2821 tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg
     2881 aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc
     2941 cgcgggcctg gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccactggc
     3001 tgcagtacgt gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc
     3061 cttgcgctta aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg
     3121 gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc
     3181 tagccattta aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg
     3241 taaatgcggg ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg
     3301 gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga
     3361 gaatcggacg ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc
     3421 cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg
     3481 aaagatggcc gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg
     3541 gagagcgggc gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg
     3601 cttcatgtga ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct
     3661 tttggagtac gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca
     3721 ctgagtgggt ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat
     3781 ttgccctttt tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt
     3841 tttttcttcc atttcaggtg tcgtgaaccg gttctagagc gctgccacca tgacgacacc
     3901 acggaatagc gttaatggta cctttccggc ggagccaatg aagggtccca ttgcgatgca
     3961 atctggaccg aaacccttgt tcaggagaat gtcatccctc gtcggtccaa cgcagagttt
     4021 cttcatgagg gagtctaaaa ccctcggagc cgtgcaaatt atgaacggac ttttccatat
     4081 agcacttgga ggactgctta tgattcctgc tggcatctat gcccctattt gtgtaaccgt
     4141 ttggtatccg ctgtggggtg gtataatgta tataatttca ggttcactcc tcgcggcgac
     4201 tgagaaaaac agccgaaaat gcctcgtgaa gggaaagatg ataatgaata gcctcagcct
     4261 cttcgccgcc atatctggta tgatattgag tatcatggat attctgaata taaaaatctc
     4321 tcatttcctt aaaatggaaa gccttaactt tatcagagcg catactccct acatcaatat
     4381 ctacaactgt gagcccgcta acccgtcaga gaagaacagc ccatctactc agtactgtta
     4441 tagtattcag tcattgttcc tgggaatact tagcgtcatg ctcatctttg cattttttca
     4501 agaactggtg atcgcaggta tcgtcgagaa cgaatggaaa agaacctgtt caagacctaa
     4561 aagcaatatc gtacttttgt ctgcggagga aaagaaagaa caaacgatag agatcaagga
     4621 agaggtagtg gggctgaccg aaacgagctc ccaacctaag aatgaagagg atattgagat
     4681 cattcctata caggaagagg aagaggagga gacagaaacc aattttcccg aaccaccaca
     4741 agatcaagaa agcagcccaa ttgaaaatga tagtagccca taaacgcgtt aagtcgacaa
     4801 atgacaagta agcggccgcg cccctctccc tccccccccc ctaacgttac tggccgaagc
     4861 cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct
     4921 tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt
     4981 ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct
     5041 ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc
     5101 ccacctggcg acaggtgcct ctgcggccca aagccacgtg tataagatac acctgcaaag
     5161 gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc
     5221 tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga
     5281 tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt
     5341 ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataatatggc
     5401 cacacatatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     5461 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     5521 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     5581 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
     5641 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     5701 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     5761 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     5821 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     5881 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     5941 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     6001 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     6061 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     6121 caagtagcgc gttaagtcga caatcaacct ctggattaca aaatttgtga aagattgact
     6181 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     6241 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     6301 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     6361 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     6421 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     6481 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     6541 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     6601 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     6661 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     6721 gcctccccgc gtcgacttta agaccaatga cttacaaggc agctgtagat cttagccact
     6781 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
     6841 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     6901 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     6961 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     7021 ggaaaatctc tagcagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt
     7081 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     7141 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     7201 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     7261 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc
     7321 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     7381 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     7441 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     7501 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     7561 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     7621 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     7681 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     7741 atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa
     7801 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     7861 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     7921 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     7981 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     8041 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     8101 tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcag cacgtgttga
     8161 caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga ggaactaaac
     8221 catggccaag ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt
     8281 cgagttctgg accgaccggc tcgggttctc ccgggacttc gtggaggacg acttcgccgg
     8341 tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc caggaccagg tggtgccgga
     8401 caacaccctg gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga
     8461 ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc atgaccgaga tcggcgagca
     8521 gccgtggggg cgggagttcg ccctgcgcga cccggccggc aactgcgtgc acttcgtggc
     8581 cgaggagcag gactgacacg tgctacgaga tttcgattcc accgccgcct tctatgaaag
     8641 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     8701 catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata
     8761 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     8821 tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag
     8881 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     8941 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     9001 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     9061 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     9121 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     9181 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     9241 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     9301 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     9361 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     9421 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     9481 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     9541 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     9601 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     9661 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     9721 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     9781 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     9841 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     9901 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     9961 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
    10021 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
    10081 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
    10141 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
    10201 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
    10261 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
    10321 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
    10381 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
    10441 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
    10501 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
    10561 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
    10621 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
    10681 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
    10741 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
    10801 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
    10861 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
    10921 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
    10981 atttgaatgt atttagaaaa ataaacaaat aggggttccg cg
//
