LOCUS       sequence-403671-        8812 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression of 2A protease from coxsackievirus B3 (CVB3). 
            Contains C-terminal His6-tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8812)
  AUTHORS   Jouppila NVV, Lehtonen J, Seppala E, Puustinen L, Oikarinen S, 
            Laitinen OH, Knip M, Hyoty H, Hytonen VP
  TITLE     Assessment of Enterovirus Antibodies during Early Childhood Using a 
            Multiplex Immunoassay.
  JOURNAL   Microbiol Spectr. 2023 Jun 15;11(3):e0535222. doi: 
            10.1128/spectrum.05352-22. Epub 2023 May 25.
  PUBMED    37227147
REFERENCE   2  (bases 1 to 8812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microbiol 
            Spectr."; date: "2023-06-15"; volume: "11(3):e0535222. doi"; pages: 
            " 10.1128/spectrum.05352-22. Epub 2023 May 25"
COMMENT     Sequence Label: pBVboostFGII WPRE 2A-CVB3
FEATURES             Location/Qualifiers
     source          1..8812
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      3..457
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(90..109)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     299..320
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(join(585..1919,1920..2006))
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial 
                     growth sensitive to sucrose"
                     /label=SacB
                     /note="negative selection marker"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     promoter        complement(2007..2451)
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     rep_origin      2528..3116
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3017..3036
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3270..3287
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  3421..3645
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(3712..4245)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(4434..4439,4440..4456,4457..4462))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        4814..4905
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     4830..4847
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     polyA_signal    5064..5198
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5088..5107)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5142..5161
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      complement(5346..5393)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     5463..5482
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    5481..5536
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     5582..5601
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     misc_feature    5686..6274
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             5792..5803
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     6201..6221
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     primer_bind     6287..6303
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     6288..6304
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(6379..6402)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             complement(6409..6441)
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /label=HSV tag
                     /translation="QPELAPEDPED"
     protein_bind    6535..6559
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(6566..6583)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    complement(7052..7076)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(7105..7214)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     protein_bind    7230..7254
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(7254..7273)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7255..7273)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7315..7334)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(7396..7418)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     promoter        complement(7697..7974)
                     /label=chicken beta-actin promoter
     enhancer        7980..8283
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     mobile_element  8466..8631
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
       61 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
      121 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
      181 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
      241 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tcttaatagt
      301 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
      361 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
      421 aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg
      481 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
      541 gatctgtcaa tgccaatagg atatcggcgt tttcttttgc gtttttattt gttaactgtt
      601 aattgtcctt gttcaaggat gctgtctttg acaacagatg ttttcttgcc tttgatgttc
      661 agcaggaagc taggcgcaaa cgttgattgt ttgtctgcgt agaatcctct gtttgtcata
      721 tagcttgtaa tcacgacatt gtttcctttc gcttgaggta cagcgaagtg tgagtaagta
      781 aaggttacat cgttaggatc aagatccatt tttaacacaa ggccagtttt gttcagcggc
      841 ttgtatgggc cagttaaaga attagaaaca taaccaagca tgtaaatatc gttagacgta
      901 atgccgtcaa tcgtcatttt tgatccgcgg gagtcagtga acaggtacca tttgccgttc
      961 attttaaaga cgttcgcgcg ttcaatttca tctgttactg tgttagatgc aatcagcggt
     1021 ttcatcactt ttttcagtgt gtaatcatcg tttagctcaa tcataccgag agcgccgttt
     1081 gctaactcag ccgtgcgttt tttatcgctt tgcagaagtt tttgactttc ttgacggaag
     1141 aatgatgtgc ttttgccata gtatgctttg ttaaataaag attcttcgcc ttggtagcca
     1201 tcttcagttc cagtgtttgc ttcaaatact aagtatttgt ggcctttatc ttctacgtag
     1261 tgaggatctc tcagcgtatg gttgtcgcct gagctgtagt tgccttcatc gatgaactgc
     1321 tgtacatttt gatacgtttt tccgtcaccg tcaaagattg atttataatc ctctacaccg
     1381 ttgatgttca aagagctgtc tgatgctgat acgttaactt gtgcagttgt cagtgtttgt
     1441 ttgccgtaat gtttaccgga gaaatcagtg tagaataaac ggatttttcc gtcagatgta
     1501 aatgtggctg aacctgacca ttcttgtgtt tggtctttta ggatagaatc atttgcatcg
     1561 aatttgtcgc tgtctttaaa gacgcggcca gcgtttttcc agctgtcaat agaagtttcg
     1621 ccgacttttt gatagaacat gtaaatcgat gtgtcatccg catttttagg atctccggct
     1681 aatgcaaaga cgatgtggta gccgtgatag tttgcgacag tgccgtcagc gttttgtaat
     1741 ggccagctgt cccaaacgtc caggcctttt gcagaagaga tatttttaat tgtggacgaa
     1801 tcgaactcag gaacttgata tttttcattt ttttgctgtt cagggatttg cagcatatca
     1861 tggcgtgtaa tatgggaaat gccgtatgtt tccttatatg gcttttggtt cgtttctttc
     1921 gcaaacgctt gagttgcgcc tcctgccagc agtgcggtag taaaggttaa tactgttgct
     1981 tgttttgcaa actttttgat gttcatcgtt catgtctcct tttttatgta ctgtgttagc
     2041 ggtctgcttc ttccagccct cctgtttgaa gatggcaagt tagttacgca caataaaaaa
     2101 agacctaaaa tatgtaaggg gtgacgccaa agtatacact ttgcccttta cacattttag
     2161 gtcttgcctg ctttatcagt aacaaacccg cgcgatttac ttttcgacct cattctatta
     2221 gactctcgtt tggattgcaa ctggtctatt ttcctctttt gtttgataga aaatcataaa
     2281 gggatttgca gactacgggc ctaaagaact aaaaaatcta tctgtttctt ttcattctct
     2341 gtatttttta tagtttctgt tgcatgggca taaagttgcc tttttaatca caattcagaa
     2401 aatatcataa tatctcattt cactaaataa tagtgaacgg caggtatatg ttcatgacca
     2461 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     2521 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     2581 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     2641 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     2701 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     2761 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     2821 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     2881 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     2941 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     3001 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     3061 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     3121 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     3181 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     3241 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     3301 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atagaccagc
     3361 cgcgtaacct ggcaaaatcg gttacggttg agtaataaat ggatgccctg cgtaagcggg
     3421 tgtgggcgga caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt
     3481 cttaaactag acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat
     3541 actggacttt tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc
     3601 gtattaaaga ggggcgtggc caagggcatg gtaaagacta tattcgcggc gttgtgacaa
     3661 tttaccgaac aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc
     3721 ggtacttggg tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag
     3781 agccactgcg ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc
     3841 gttggcctca tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc
     3901 gccggagact gcgagatcat agatatagat ctcactacgc ggctgctcaa acttgggcag
     3961 aacgtaagcc gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa
     4021 tgtcttacta cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt
     4081 ggctacgtct ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg
     4141 gtcagggccg agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt
     4201 agggcgactg ccctgctgcg taacatcgtt gctgctgcgt aacatcgttg ctgctccata
     4261 acatcaaaca tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact
     4321 gtacaaaaaa acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc
     4381 gttcggtcaa ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagtttac
     4441 gaaccgaaca ggcttatgtc aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc
     4501 acccggcaac cttgggcagc agcgaagtcg aggcatttct gtcctggctg gcgaacgagc
     4561 gcaaggtttc ggtctccacg catcgtcagg cattggcggc cttgctgttc ttctacggca
     4621 aggtgctgtg cacggatctg ccctggcttc aggagatcgg aagacctcgg ccgtcgcggc
     4681 gcttgccggt ggtgctgacc ccggatgaag tggttcgcat cctcggtttt ctggaaggcg
     4741 agcatcgttt gttcgcccag gactctagct atagttctag tggttggcta cgtatactcc
     4801 ggaatattaa tagatcatgg agataattaa aatgataacc atctcgcaaa taaataagta
     4861 ttttactgtt ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca
     4921 taccgtccca ccatcgggcg cggatcccgg tccgaagcgc gcggaattca aaggcctacg
     4981 tcgacgagct ccttaaggct agcgtttaaa cggccggccc gatcgcctag gactagtagc
     5041 tcactaggct caagcagtga tcagatccag acatgataag atacattgat gagtttggac
     5101 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
     5161 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
     5221 ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca
     5281 aatgtggtat ggctgattat gatcctctag tacttctcga caagcttggt accgcatgct
     5341 ttcagcaaaa aacccctcaa gacccgttta gaggccccaa ggggttatgc tagttacata
     5401 tgggcatatg ttgccaaact ctaaaccaaa tactcattct gatgttttaa atgatttgcc
     5461 ctcccatatg tccttccgag tgagagacac aaaaaattcc aacacactat tgcaatgaaa
     5521 ataaatttcc tttattagcc agaagtcaga tgctcaaggg gcttcatgat gtccccataa
     5581 tttttggcag agggaaaaag atcgatctca gtggtatttg tgagccaggg cattggccac
     5641 accagccacc accttctgat aggcagcctg cacctgaggc ccgatgcggg gaggcggccc
     5701 aaagggagat ccgactcgtc tgagggcgaa ggcgaagacg cggaagaggc cgcagagccg
     5761 gcagcaggcc gcgggaagga aggtccgctg gattgagggc cgaagggacg tagcagaagg
     5821 acgtcccgcg cagaatccag gtggcaacac aggcgagcag ccatggaaag gacgtcagct
     5881 tccccgacaa caccacggaa ttgtcagtgc ccaacagccg agcccctgtc cagcagcggg
     5941 caaggcaggc ggcgatgagt tccgccgtgg caatagggag ggggaaagcg aaagtcccgg
     6001 aaaggagctg acaggtggtg gcaatgcccc aaccagtggg ggttgcgtca gcaaacacag
     6061 tgcacaccac gccacgttgc ctgacaacgg gccacaactc ctcataaaga gacagcaacc
     6121 aggatttata caaggaggag aaaatgaaag ccatacggga agcaatagca tgatacaaag
     6181 gcattaaagc agcgtatcca catagcgtaa aaggagcaac atagttaaga ataccagtca
     6241 atctttcaca aattttgtaa tccagaggtt gattatcgga attccctcga ggtcgacggt
     6301 atcgcgataa gcttgatatc gaattcgaag cttgagctcg actagctagc tagctactag
     6361 cctgaggtta atcacttagt gatggtgatg gtgatggtgg tgctcgagat cctcggggtc
     6421 ttccggggcg agttctggct ggcttgcacg tgtatacagc tgtgcggccg caagcttcga
     6481 accggtaccg tcgacctgca ggcgcgccga gatctgagaa ttcggatcga tatcaccact
     6541 ttgtacaaga aagctgggtc tattaatggt ggtgatgatg gtgctgttcc attgcatcat
     6601 cttccagcca caggagatca cggatgtctg caaagccgac cacgccttca ccccccatgg
     6661 tcacaatgcc aatgacacca tgctcacacc ttaggatacc gccacagtca cctggttcgg
     6721 aaaatccagc tgctaaaagc acatgggatt ggtatctcct ggggtagtat tcactctctt
     6781 ggacctctac tagacctggt ccttcaaacg aaattgggta gtgcttgttt ttggacgcac
     6841 aaaagtacac tcccgttgtg cactgacatc tggctataat atcacatcca tgtgctgtgg
     6901 tcgtgctcac taagaggtct ctgttgtaac tttcccacac acagttttgc cagtcagcac
     6961 tggtagctag atgtctattt accaccctgt agttccccac atacactgcc cctgattgtt
     7021 gtccaaatgc gcccatggtt ctatctcctt cagcctgctt ttttgtacaa acttgtgata
     7081 tcggatccgt ggtatatctc ctttgattgt aaataaaatg taatttacag tatagtattt
     7141 taattaatat acaaatgatt tgataataat tcttatttaa ctataatata ttgtgttggg
     7201 ttgaattaaa ggtcccggat taactccggg gaattgttat ccgctcacaa ttcccctata
     7261 gtgagtcgta ttaatttcgg tccgatccaa ttctttgcca aaatgatgag acagcacaat
     7321 aaccagcacg ttgcccagga gctgtaggaa aaagaagaag gcatgaacat ggttagcaga
     7381 ggctctagag ccgccggtca cacgccagaa gccgaacccc gccctgcccc gtcccccccg
     7441 aaggcagccg tccccccgcg gacagccccg agccgctccc cccgcacaaa gggccctccc
     7501 ggagcccctc aaggctttca cgcagccaca gaaaagaaac aagccgtcat taaaccaagc
     7561 gctaattaca gcccgaagga gaagggccgt cccgcccgct cacctgtggg agtaacgcgg
     7621 tcagtcagag ccggggcggg cggcgcgagg cggcggcgga gcggggcacg gggcgaaggc
     7681 agcgcgcagc gactcccgcc cgccgcgcgc ttcgcttttt atagggccgc cgccgccgcc
     7741 gcctcgccat aaaaggaaac tttcggagcg cgccgctctg attggctgcc gccgcacctc
     7801 tccgcctcgc cccgccccsc cccycgcncc cgccccgccc cgccntggcg cgcccccccc
     7861 ccccccccgc ccccatcgct gcacaaaata attaaaaaat aaataaatac aaaattgggg
     7921 gtggggaggg gggggagatg gggagagtga agcagaacgt ggggctcacc tcgaccatgc
     7981 atggtaatag cgatgactaa tacgtagatg tactgccaag taggaaagtc ccataaggtc
     8041 atgtactggg cataatgcca ggcgggccat ttaccgtcat tgacgtcaat agggggcgta
     8101 cttggcatat gatacacttg atgtactgcc aagtgggcag tttaccgtaa atactccacc
     8161 cattgacgtc aatggaaagt ccctattggc gttactatgg gaacatacgt cattattgac
     8221 gtcaatgggc gggggtcgtt gggcggtcag ccaggcgggc catttaccgt aagttatgta
     8281 acgcggaact ccatatatgg gctatgaact aatgaccccg taattgatta ctattaataa
     8341 ctagagcaac tatatattga tagacatttc cagtttgtga tattagtttg tgcgtctcat
     8401 tacggccggg catgcctcga gactgcaggc tctagattcg aaagcggccg cgactaggag
     8461 atccgaacca gataagtgaa atctagttcc aaactatttt gtcattttta attttcgtat
     8521 tagcttacga cgctacaccc agttcccatc tattttgtca ctcttcccta aataatcctt
     8581 aaaaactcca tttccacccc tcccagttcc caactatttt gtccgcccac agcggggcat
     8641 ttttcttcct gttatgtttt taatcaaaca tcctgccaac tccatgtgac aaaccgtcat
     8701 cttcggctac tttttctctg tcacagaatg aaaatttttc tgtcatctct tcgttattaa
     8761 tgtttgtaat tgactgaata tcaacgctta tttgcagcct gaatggcgaa tg
//
