LOCUS       sequence-403957-       13828 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  rpl-21 promoter-driven C04F12.1 construct tagged with 2xHA for 
            MosSCI integration at locus ttTi5605 on Chr II in nematode.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13828)
  AUTHORS   Tabara H, Mitani S, Mochizuki M, Kohara Y, Nagata K
  TITLE     A small RNA system ensures accurate homologous pairing and unpaired 
            silencing of meiotic chromosomes.
  JOURNAL   EMBO J. 2023 Jun 1;42(11):e105002. doi: 10.15252/embj.2020105002. 
            Epub 2023 Apr 20.
  PUBMED    37078421
REFERENCE   2  (bases 1 to 13828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2023-06-1"; volume: "42(11):e105002. doi"; pages: " 
            10.15252/embj.2020105002. Epub 2023 Apr 20"
COMMENT     Sequence Label: pHIT318
FEATURES             Location/Qualifiers
     source          1..13828
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1491..1502)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             4290..4316
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             7094..7105
                     /codon_start=1
                     /gene="cspA"
                     /product="translation enhancing element for E. coli (Qing 
                     et al., 2004)"
                     /label=TEE
                     /translation="NHKV"
     primer_bind     11813..11835
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(11873..11891)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11959..12063
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12064..12132,12133..12924)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12282..12301)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      13095..13683
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13584..13603
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gggccctgtc ggattatggg ctcttctttt atcctctact ttctttctag aaaatgcaaa
       61 tgttgagtca gaaattgctg aatggagtac aactgcaatg ggtttagctt atgtttctgg
      121 aacaattact ggtggaattg tgattgaaaa gtaaggttat atttcatttc aataaatgtt
      181 accacagtat aacaaaaaca agttacattg ttcagagtat gtctgaatgt attattatta
      241 ttgtatttgt gcatcagtac tgtagttgta ctgaagaagg gtttaaaata atctcaacgt
      301 ttttaagacg agttagtgag aatacggtcg cagttaggat gtttggacat agttttatta
      361 gataatcgtg aactaactca ctataaaaac ccgctaactc attgaataag atagttttaa
      421 acgttcaaaa tttgaagaca aaattgtatg aataagttta cagagttggt cgacgcaagc
      481 ttctccttct cttcacattt ctcaataatt tagctctaat tgcattcgta ttcttcgcaa
      541 aaatacgtat tctgattgac ccgatgaaat acggatgcct agttgcattg atcatctacg
      601 gttatactta tgggtgaggt ttttcaaata tattttgatt taatcgaata atgaaatttc
      661 agaactggag ttggtccaat ttcgtggttt atatcctctg aactggtacc tcaaaaacat
      721 cgtagtattg ctcaatctgt ggcatattca atgaatactg taatggtggt tatcagtaca
      781 tttacagtat tacctctata ttccgttatt ggtagttatg ctttccttat tctatattca
      841 attccttcat ttataagtat gcttatatta tttcgctact taccggaaac caaaggacga
      901 gagattcatg atattgtgaa tgaacttaaa aaaaaatgaa aacctttgaa aacgttcata
      961 cggccgaaat tttacttgat cgagccaatt gaacttttgt taagacttac agaaaagtga
     1021 gagattttgt tacaaaaaag tgagaaaaaa gcgaaaatga acaggaaaaa gaagaaaaat
     1081 acacccgaag tatcatataa aaattaaaaa gattgtttga cctggcggaa cttttttgca
     1141 aaaactctga atatccatgg cacatgcttc gtgcaaaaca ggatccaaat gaatatcaac
     1201 aagagattct tgaagacgac gagccacttg ctcagagcat tgcttatctc gaatgagacc
     1261 cttgttaaaa tcatttttca aacattatat gacattgtcg aaatgtcctc ctgattccat
     1321 gatggtagca aactcacttc gtctttgagc caatttatcc aagtccttgt aaaaagtatt
     1381 cgaaaattgt taacggataa aatgtttatt ataatatcaa aaacaatttg tcagttgacc
     1441 actttttgat atatttgaca gaaaccggga tgaattggct caaagttagg gcgcccttct
     1501 attacagggt ttctgataac aaaccggtta ttaactccca acaagggatg atttcaattc
     1561 atcatgctca aattgaccca aattaagtta catgacaaat tcatcgccct tttcaactct
     1621 tcttggctca tcatctgtta ttctgttcct attctgtctg caccctatac ccctttgcat
     1681 actctctcgt cattcctttc ttggatagtg tcttcttctc cccagctctg ctacttctat
     1741 gacttgcgcc cgctgctttt ccgctcgttc ctctcctctc tgacgtcttc ttctgcttct
     1801 tttctccttc ctttttccct tctcaatctt cctctttttc ccatttctgt caatcattcg
     1861 aagaagaaga agaagaagac cctcattcat ttcatttttt tttctgtcgg tgtgtgctgc
     1921 tcggttaaga gtgagctctc tattccacgt cttcttcttt ttcttcttcg attcgaatca
     1981 atcactccac caaaacgcat tcgtttttgg gattcacccc gcggttcgca ataggtttct
     2041 ttttcaaatt attagcgtta taaatagaaa aatggtggag tttcaataaa aatgataatt
     2101 tacaaagtgt attttgatta catgtactca aaaaggttga aatttttcat accagttttc
     2161 cggaaatcca tctgatatca ttatcgtatt tttcttttta aaaatgtttt caaaaaaaaa
     2221 caaaatatag ctggttattt ggcaccctct aattaccatt ttcctgtcac accacactct
     2281 tttccttctt ccacttcttt ccgttttcag ccgcttccaa ccaaaccgat atgaaagccg
     2341 agcaacaacg atcgattcca cccggctcgg cgaccttccc gtcgcaggtg agactcagaa
     2401 aactagagaa accgctcaac taactcttga tatccgattt cattcttttc tcttttcttt
     2461 ttgttgaact tttccatatt tccagatgcc acggccacca ccaagcaccg aacaaggaat
     2521 cacaacggaa tcggagcttg cgaagaaagc tcaaatcact ccgaacgacg ttttagcact
     2581 tccgggaatc actcaaggta tcttgctccc tttggatttc agtttaaaac ataaatttag
     2641 gattcttatg ctccccatct gcgaacatct ataacatcga gttcaccaag ttccaaatcc
     2701 gtgatctgga cactgagcaa gtgcttttcg agatcgccaa accggagaac gatcaggaga
     2761 atgatgagtc gccacaggag tcggcaagat acgtgcgtta tagatttgct ccaaactttt
     2821 tgaaactcaa aacggtcgga gcaactgtgg aattcaaagt aggagacatc ccaatccatc
     2881 atttccgaat gatcgaacgt cacttcttca aagatcgcct tctgaagtgt tttgactttg
     2941 aattcggatt ctgtattccg aattcacgaa acaactgtga acatatctat gagttccctc
     3001 aactctctca acaactcagt gagtcattat tctaaaaagt acaattcaaa agactaatct
     3061 ctttttcagt ggacgacatg atcaacaatc caaacgagac tcgttctgac agcttctatt
     3121 tcgtcgataa caaactcgtc atgcacaaca aagccgacta ctcatatgat gcataaatat
     3181 ttaatacaaa aatgttctgg ataattattc tgtcgaatag aaaaaaaact ccaaatgtga
     3241 ttaaattcca ataattcctg tctagtttgt tccttcccct tccccttctc atgttcaatg
     3301 cattcctaag cttttcagtt ccccccttgt ttctatattt tttcgctgtc ctgtcacact
     3361 cgctaaaaac actaatcaca cggaaatctg ttttcaataa aaactccaac tttaactcat
     3421 tttcaatttc aactgaaaga ttttttcatt agagaatgtc tagaactagg cccgggctac
     3481 gtaatacgac tcacttaacc atacgcgtcg gtgagccctt ccaaagtgta gttgaaggtg
     3541 taggctccga aggcggaagc caaaaggatg gtggagaaac ggcgagaaac ggtgttgtag
     3601 acgatggtac ccagcgacat tctgaaatat acactacttt caatagatgt agaatacata
     3661 cattaaataa tacaacaaaa attgaggaat ttctacacgg aaataaaata atcaaaagaa
     3721 aactgtttga aagatcttat tcaaggaatt caatgattaa ttgctagtac tagatcgaga
     3781 aaaatgggaa aagttattaa aataaaactt tgttattaga aaaaccgtct gaaaaaaaaa
     3841 ccgaataaaa atggaggcga gagacgaaaa aacgtgcgga actcttaagg agacgcagca
     3901 tgtctgacgg tttggtctcg cagcgatcta tgggaagcgc gacacacttg aaacgagtgt
     3961 taccgtactg tgtgccttta aacaattttt atgctactgg cctaaagatt ttttctcttt
     4021 ttttccgtaa aaactgtgaa aaatagtgaa attttcgtca atttttatgc ctctgtgtaa
     4081 aaacttgcta atctttctag tatttaacga actctgcctt agtttttgct gaaatatgtt
     4141 caaaaaatcg atttttcgtt catgtgcttt ttttgtaaat gtttctcggc aaattgtttt
     4201 acatttttgt tcctcgtagt tctctatatt gacacctcta tcaaaaattt ttttcagggt
     4261 taaatatgga cacctatcgt tacattggat acccatacga cgttccagac tatgcggccg
     4321 catatccata tgacgtccca gattacggac catcaaagaa gaacaaaaag gccgctgcag
     4381 ctgaacgatc agcaacactt tctgcttctg catctcaaca agttgcaaag ggtaagtggt
     4441 ttttttcaac tttacataaa tcaaattggc aagcaagtct tttgatttta tttttttttc
     4501 tggagttttt gaagatttct tgaaacgatt gaaaagtctt aacttttcat aacgcaacaa
     4561 ctgttcctga aaaacataaa gaacgagtca aatttcaaaa aaaacccagg ataattctag
     4621 aaaattgtga acaacttggg gtacggtaga tacgaagttt tggtcgtttc gtgattataa
     4681 aatagagcaa tatgattcga agagtgggaa gtaccaaata gttattccat aaatatctga
     4741 gaacaaaatt ttggaaatca tgaaatttca agcacacgtg cttcgccgtt tttattcagg
     4801 gccatcaaaa tagctttgaa gtatgtttta acacattcta ttttgaatat agaatatttc
     4861 attttaattt ttcccaaagt ctcaatagtt ttaagatttt gcgttgtatt atttaaatcc
     4921 gaaagctcta aagttaggag ataaaatatt ttttccagac gagcaatcag ctccagtagt
     4981 tccaactcct gaattgtctc gtgtctctgt ttcctccaat cctccgactc cttctgcaat
     5041 tgaaaaacct gtccctcttc ctgttccatc gaaaccattg ttcgagtacg aacctccagc
     5101 acaactggaa gctttggcta agaataagcc tgtgaaagtg acaaccaact catacgaact
     5161 tgagttgaag ccaactcctg tttttcgtta cgacgtcagt gttctgaaga gctttgacga
     5221 ttcaaccagg gatccaattg agcttgctgg ctcgaaaggc ggcgatcgtc agcggcaagc
     5281 tgacttgacg gaaatcgtga aagttgctct cacattggaa ggccttggaa atctgaacat
     5341 cttctacgat ggcgcagcga tgcttttcac aactcagaaa ttcgaaacaa aaacggaaaa
     5401 gcagcaagca attttcatgc caattccgtc tgatcatcca aagctctcaa catctctcaa
     5461 agaaagccaa ttcggaagag cttccggaaa gttccacgtg tccatcgagt tgaactcgaa
     5521 acaatcggaa ctaaatacaa tcgatcttct caatgaacgt ctggattcag cagcttgtcc
     5581 agtcactcag atggctcaaa tcgctctgag tgcagaggcc aaacaaaatg gcttcatcat
     5641 tttggatgga ggacatgaac tcttcgatca gaagaataca agaagaacaa ttggaaaaga
     5701 tggagttgag gatatggatg gagtggctgc cagtataaaa atggctcaag gaaataagaa
     5761 aactggatct gcacacttgg tcatggactg taagttctca ttttactttt atatgattgt
     5821 ttaaagatgg attataactt atgagaattt tgtttttaac ttttttaaag acttccctga
     5881 aactagttgt gtgttccatt ctcatttaaa ataaatataa tttcagacaa aaagaagcag
     5941 ttcttcaaga ctggtccatt gaaggatttg ataaaaggaa tcaatccaaa tcagctccgt
     6001 tcatatctca aaggtcttcg tgtccacact acatactctc atcaatcaat cttcattgat
     6061 ggaatttcca atgtcccaat cggagaaatt aaacttcccg atggtactcc tcttcttgat
     6121 gattgtgctc gagtttccgg aaagcccaag agcttttttg atgcttccaa gcctgcggtt
     6181 caagttaacc agtacaacaa acgggaaggt agacgtatgg tttatagctt ccctatcgag
     6241 aatttactgg taaaaccaaa ccagaaactg actcagaacc atggagaacc accgagaagc
     6301 atcaagccgg agctgagatt tgatctcata agaaaggttg gagagtctgc aaagcttctc
     6361 tctccaaatc agacactgaa atcaattggt attgcgatca atccagaacc gatcgttgtt
     6421 gaagctatga ctgtgccaaa gccaaccatt ctatacaaag acggagcatt tacttctcca
     6481 gatctgttaa acagagtagg ttattcaata gtgtttcatt aataatattt tgaatttcag
     6541 acatcatggg acgttcaagc acgtaaccac caatctctaa aaggcggatt tgtggaaccc
     6601 atgaagatca acaagatctt gattctgtat aatagtcaat cctacagtca agcaagtcca
     6661 aaagaagcag ttgtcgaact gcaatcaatt ctcagcaaaa aagccaaaga tgttggaatg
     6721 acaattgggc aaattgatgt ggaagatctc aatgatccgg atatcatcgg agcaattgaa
     6781 aacgtattta taatgttaat gctgttcaac ccagcttatg tattttcaga aaatgaccat
     6841 tctaaaaacg agccaagtca agccaattgt gatctacgcc gatcatacta gtgagggtac
     6901 ccacagcact ttgaagcttc aggaacgatt gtgtgaggtc ataactcaac aagtcgcttt
     6961 agacaaatca ttgaaaagaa caccgtaagt ttggaactcg gaaattcatt cagcaatttt
     7021 tttttaattt ttcagtggaa aagtgacagc aaacaacttg ttgatgaagc tcaatctgaa
     7081 gtatggtggc gtgaatcaca aagtgcgagt ggacaatagc atgtatgtta atttttttag
     7141 aattcaaagt tttctgacat tatttccaat ttcatgcaca atcagttttc taaagatgct
     7201 tcctgctaca tttttgtaga actttcgtat caaaagtaaa tcaatgaagc atcaatgatt
     7261 taagaaaacg ttttacccat catatacttc accattcttc tttgaaattg aaacattttc
     7321 agttctcatc tctggggaga cacttcaaac acactcatca tctcatatga tgtgtgccat
     7381 tcttctggaa aagtgtacaa gaaggatgag atttgtgacg agccaagctg cgttggtgta
     7441 agttttcaat ggaaaaaaaa acataatccc tagattcaaa tatcacttct gttattatag
     7501 catgatttgg tattcctaac tactgagagt tatttaagaa attcaaaagc aaaattttgt
     7561 ttccaaataa aaaaaattgg atagcatgga ttaggcgcgt agacagataa ttatgaagta
     7621 gaataaggaa agatagacat gatgtagata tgagctagac acgtaggtag gcataaagta
     7681 ggcatcagac aagcccgata tcacaataat tgtaggcccg tacgcctgat tagtaaaaca
     7741 ggttttattg gtttttgagc aaaggcaaat tttgaagacg aagacccggt tttccgtccg
     7801 tactatagga cttttgaaat gattctggaa tattttcagt ttggattcaa tggaaccgct
     7861 tgccccgaag cattcattgg agatttccat taccagctcc cacgtcacga acaagttgat
     7921 gaacatcttc tgaaattgag agccagattc atgctcaatc actatattac atctcgtaag
     7981 aagtatcccc aacaagtggt cattttgcgc gacggagtct ctgaaggaca acacaaaatg
     8041 gtcagaaacg aagagttcca ggcgattaaa cagagcatca tgaatgtttt caatgaaaag
     8101 aaggcaaagg ctccgtcttt tgctcttctc gttgtcacta agcgtcatgc caatcggctt
     8161 ttgattaaag atcagaatgg taagaatttt tgaaacatgt tctaaacata tgtaaataac
     8221 tttcgttgaa acttgaatag cgtagccgtg aatctgcggt atgcggctac aaaacatcag
     8281 tttgatagtt ttctattcgt tattattaac ttttgatata agcgttgcaa tatcctaagt
     8341 tatgaaatga gcagtttttt agaattgttc tgaacatttg ccacacaatt ctgataggca
     8401 tggaagaagc ataaactttt gttattaagt ggaatgtctt gataaatgcc aaatgctgaa
     8461 cttactatac atcttcaagt ccttaacatt gagcttattg ttcatataat tttataaaca
     8521 gtttcaggat atacgaatgt cccaccgttg acggcaattg accaaacaat tgtgaagaag
     8581 caaggaaatg aagtcatctt tgtgtcccat tgtccactca atggtaccgc ccaaccaatt
     8641 gtaatcaata cattggtcaa cgatggaatt ttcaagacca atgatcaatt ggtccagttc
     8701 atcacagcac tttgctgtgc tcatcagaag tctacaaaca tagtgtctct cccagagagt
     8761 atctacgcag ctgacgaata cgccaaaaga ggagccgatt tgttccagtc gtataaactg
     8821 ttagttgttc tacaaccaat gctttaaata tcataattaa atctttcagg aagcatcgag
     8881 atcttccaac gactacgatc gacgactcga cccagctcga ctacgaaaaa atcaccctaa
     8941 ttctctgctt ccaaacgagc tctttcaaaa ataagagaat tgcttaaatt aacgttttaa
     9001 acttctatac agcttccatg tactcaaaca attttgaatg tattactttg tcactcatta
     9061 ctctactttc cggtttaatt ttaaaacgga aataattatg tttagttgtg aactaaactc
     9121 tgtgcttaaa gacaataaaa cttttttatg aggacaaaat aaagttttga aagttagaat
     9181 tgtaagtatt aagtttgaaa aattaaaaat ctaactttac aacaaagttc agaaaaattt
     9241 gccgaacagt tcaaacaaac tttagtaata aagaaggagt acagttaggg gtactgtata
     9301 aaaacaaact tacaagccag acggcacggc gagaaccttc taatgttttt tgtttaccaa
     9361 aaaaggactt gcccctcagt tgaaaattga gaaattatgg cattcaactg acacaaatat
     9421 attcattgat ttgaaatatc ctgttgatgt caaggaaatg aacgtttgtt atcataaaca
     9481 gtaaaaagaa aataaattta atgaacacac tttccaattt tgggaaaact taccagaatc
     9541 gtaccaggtc ttatgaatgg aaaacggaag aattcatgag atttagtgct acaaaaaaat
     9601 ttcaaaatgt ggaagattca caaaagctaa gaaatgtgat ttgtaatttt tttttgaaat
     9661 aagatttata atgttattag atttaaagtt tcgaagtttt aaggatctat taaactatta
     9721 caaactaaga taaatcaatt cttacggaat tatccaactt ctcgataaat caattttgtc
     9781 gacgaaacga tcatcataaa ttatttgttt tctcgacttg attgtagttt tgaacagatg
     9841 agatttcatc agaattcaca gaatttcttg cagaagccac accttttgtt aacttattta
     9901 aattgaggat attggcttgt tccttcagct agtatttttg aaatttcatt ccgtgattat
     9961 tttcattccg tgattatttt cactcgggaa tcgctcgccc agcatgcgat cgcgtcgaca
    10021 gatctgccca ctagtgagtc gtattataag tgcaagtaag atcagtgttt gtttcgatat
    10081 cagtctgttt cgtaacggtc ttctgtataa ctacaaaaaa gaataaaaaa ccgtatcaca
    10141 actgagaaaa aagacaaaac ttattgaaca gtgttccttt caaaaaatta ttttgtaaaa
    10201 aatcgagttt ttctccaaaa taacgcacgc aatcaaaaac aagaaaacta acgtgttctc
    10261 ctgggcgtgg aactccttat caagattacc attattgtct gtcagaattg atatttcaat
    10321 tatttcatat ctagacaaac ccgggtgtct tgcatttatt agtactgtga gtttacatac
    10381 aaacgaggct tactcagata acatagagtc acctctaatt agcaaaaacc catttctaat
    10441 tagctatttc gaccagttct aaagtcaccg taattagaaa tggtttttgt agcttttcta
    10501 attagaaatg accccgagat aagtgaaata tggcattttc tgggttttct agtaagaaat
    10561 gatcccgcac gtagtgattc cgcgttacaa gccaaattca aattaaaaaa gtatattaca
    10621 caattcaagt tacattattt tcaggaaaca atggcgaaga gcaaagcaga gattcaaaag
    10681 gaggaaagag acaacagata tgaacgaaga cttcttgaaa agagaaagag acatgaggtg
    10741 agtaaccatg ataataaata atcaagtaag ggctatagat ttataaattc tggagttaac
    10801 agtttcgaaa aaaagtagtt ttcatatgtg cgaaggaata tcaaaaatag gggaatagcc
    10861 taaacgagtg agaaaattaa atgtttgtta ttttttcaaa aatgtctgca gggccgttat
    10921 cctttcaaat gcactctatc aaacatagat catatccatc atatcttatc cagaatttat
    10981 atcatatcca agattgagag cgtttcaagg aaaatctcaa acaaccatac aattttgtag
    11041 atcgacaaca aggcctccac ttcttcatcg atggcctctg cagcacctgc accaccaagt
    11101 ttgtattttt catattaata taaagtgagc cacgcatagt cgtttttagg atttttgcca
    11161 gatcgatgtc gcgcaaacag actgtttttt tttctttgaa agccgtgcgt gacatgggcc
    11221 acggattgta acgccgcgct ggatggataa aaatttattc agttttttag cctttacaaa
    11281 atatggttcg actactttct ttatttttac atttttatag gtgctcattg agctgagata
    11341 ttttgggtat aatcggaaaa tcgctgattt tggtcatttt tctgcactaa agattacaca
    11401 aaaagttcag tatttagatt cattgttgaa aattggggat gatctacaga tacatatcgc
    11461 cgtgttcaaa aacacacgtg ctgtgacgtc gtgactgggg aaaaccctgg cgttacccaa
    11521 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
    11581 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat
    11641 tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtgcaatc
    11701 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc
    11761 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
    11821 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg
    11881 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
    11941 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
    12001 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
    12061 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
    12121 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
    12181 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
    12241 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
    12301 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
    12361 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
    12421 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
    12481 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
    12541 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
    12601 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
    12661 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
    12721 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
    12781 tctcgcggta tcattgcagc actggggtca gatggtaagc cctcccgtat cgtagttatc
    12841 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
    12901 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
    12961 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
    13021 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
    13081 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
    13141 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
    13201 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
    13261 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
    13321 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
    13381 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
    13441 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
    13501 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
    13561 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
    13621 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
    13681 aaaaacgcca gcaacgcggc ctttttacgg ttcctggtct tttgctggcc ttttgctcac
    13741 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
    13801 gctgatacca gcggataaca atttcaca
//
