LOCUS       sequence-404004-       10226 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Heterologous expression in mammalian cell lines or Xenopus oocyte 
            (T7 RNA pol).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10226)
  AUTHORS   Wei AD, Wakenight P, Zwingman TA, Bard AM, Sahai N, Willemsen MH, 
            Schelhaas HJ, Stegmann APA, Verhoeven JS, de Man SA, Wessels MW, 
            Kleefstra T, Shinde DN, Helbig KL, Basinger A, Wagner VF, 
            Rodriguez-Buritica D, Bryant E, Millichap JJ, Millen KJ, Dobyns WB, 
            Ramirez JM, Kalume FK
  TITLE     Human KCNQ5 de novo mutations underlie epilepsy and intellectual 
            disability.
  JOURNAL   J Neurophysiol. 2022 Jul 1;128(1):40-61. doi: 10.1152/jn.00509.2021.
            Epub 2022 May 18.
  PUBMED    35583973
REFERENCE   2  (bases 1 to 10226)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1152/jn.00509.2021"; journalName: "J Neurophysiol"; date: 
            "2022-07-1- 1"; volume: "128"; issue: "1"; pages: "40-61"
COMMENT     Sequence Label: hKCNQ5(WT).ires.GFP-pcDNA3
FEATURES             Location/Qualifiers
     source          1..10226
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(155..172)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     382..401
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(575..577,578..580,581..1294)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(575..577,578..580,581..1291)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(620..641)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(881..900)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     promoter        complement(1310..1328)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1311..1328)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1348..1365)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1354..1578
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      1624..2052
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1711..1730)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1921..1942
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2061..2081)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2066..2395
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2246..2381
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2308..2327
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             2462..3256
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2516..2535)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3126..3145
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    3430..3551
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3467..3486)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3521..3540
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3600..3616)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3600..3616)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3613..3635)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3624..3640
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3648..3654,3655..3672,3673..3678))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3693..3714
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3831..3848)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4002..4590)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4082..4101)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4761..5552,5553..5621))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5384..5403
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5622..5726)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5801..5820)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        5992..6371
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6372..6575
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6526..6546
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6620..6639
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6620..6638
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     5'UTR           complement(6677..6719)
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     regulatory      6781..6790
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    join(10214..10226,1..562,563..565,566..574)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
ORIGIN
        1 ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat
       61 atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct
      121 gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg
      181 ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta
      241 gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag
      301 ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg
      361 atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat
      421 gcccagaagg taccccattg tatgggatct gatctggggc ctcggtacac atgctttaca
      481 tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc
      541 ctttgaaaaa cacgatgata atatggccac aaccatggtg agcaagggcg aggagctgtt
      601 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
      661 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
      721 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt
      781 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
      841 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
      901 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
      961 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
     1021 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
     1081 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
     1141 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag
     1201 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     1261 gatcactctc ggcatggacg agctgtacaa gtaatctaga gggccctatt ctatagtgtc
     1321 acctaaatgc tagagctcgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
     1381 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     1441 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     1501 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
     1561 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc
     1621 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     1681 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     1741 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     1801 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     1861 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     1921 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     1981 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     2041 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     2101 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     2161 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     2221 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     2281 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc
     2341 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     2401 cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg
     2461 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     2521 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     2581 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     2641 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     2701 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     2761 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     2821 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     2881 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     2941 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga
     3001 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
     3061 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     3121 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     3181 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     3241 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac
     3301 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     3361 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     3421 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     3481 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     3541 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg
     3601 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     3661 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     3721 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     3781 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     3841 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     3901 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     3961 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     4021 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     4081 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     4141 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     4201 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     4261 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     4321 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     4381 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     4441 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     4501 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     4561 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     4621 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     4681 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     4741 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     4801 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     4861 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     4921 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     4981 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     5041 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     5101 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     5161 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     5221 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     5281 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     5341 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     5401 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     5461 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     5521 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     5581 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     5641 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     5701 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac
     5761 ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctgc tctgatgccg
     5821 catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga
     5881 gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta
     5941 gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt tgacattgat
     6001 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
     6061 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
     6121 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
     6181 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
     6241 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
     6301 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
     6361 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
     6421 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
     6481 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
     6541 ggcgtgtacg gtgggaggtc tatataagca gagctctctg gctaactaga gaacccactg
     6601 cttactggct tatcgaaatt aatacgactc actataggga gacccaagct tatcgatacc
     6661 gtcgacgcta gctctgccaa agttgagcgt ttattctgag cttctgcaaa aagaacaaga
     6721 agggtaccga gctcggatcc actagtaacg gccgccagtg tgctggaatt ctgcagatat
     6781 ccatggcggc cgccgccgcc atgccccgcc accacgcggg aggagaggag ggcggcgccg
     6841 ccgggctctg ggtgaagagc ggcgcagcgg cggcggcggc gggcgggggg cgcttgggca
     6901 gcggcatgaa ggatgtggag tcgggccggg gcagggtgct gctgaactcg gcagccgcca
     6961 ggggcgacgg cctgctactg ctgggcaccc gcgcggccac gcttggtggc ggcggcggtg
     7021 gcctgaggga gagccgccgg ggcaagcagg gggcccggat gagcctgctg gggaagccgc
     7081 tctcttacac gagtagccag agctgccggc gcaacgtcaa gtaccggcgg gtgcagaact
     7141 acctgtacaa cgtgctggag agaccccgcg gctgggcgtt catctaccac gctttcgttt
     7201 ttctccttgt ctttggttgc ttgattttgt cagtgttttc taccatccct gagcacacaa
     7261 aattggcctc aagttgcctc ttgatcctgg agttcgtgat gattgtcgtc tttggtttgg
     7321 agttcatcat tcgaatctgg tctgcgggtt gctgttgtcg atatagagga tggcaaggaa
     7381 gactgaggtt tgctcgaaag cccttctgtg ttatagatac cattgttctt atcgcttcaa
     7441 tagcagttgt ttctgcaaaa actcagggta atatttttgc cacgtctgca ctcagaagtc
     7501 tccgtttcct acagatcctc cgcatggtgc gcatggaccg aaggggaggc acttggaaat
     7561 tactgggttc agtggtttat gctcacagca aggaattaat cacagcttgg tacataggat
     7621 ttttggttct tattttttcg tctttccttg tctatctggt ggaaaaggat gccaataaag
     7681 agttttctac atatgcagat gctctctggt ggggcacaat tacattgaca actattggct
     7741 atggagacaa aactccccta acttggctgg gaagattgct ttctgcaggc tttgcactcc
     7801 ttggcatttc tttctttgca cttcctgccg gcattcttgg ctcaggtttt gcattaaaag
     7861 tacaagaaca acaccgccag aaacactttg agaaaagaag gaacccagct gccaacctca
     7921 ttcagtgtgt ttggcgtagt tacgcagctg atgagaaatc tgtttccatt gcaacctgga
     7981 agccacactt gaaggccttg cacacctgca gccctaccaa gaaagaacaa ggggaagcat
     8041 caagcagttg gggacagtgg acattgcgtc agaagctaag ttttaaggag cgagtgcgca
     8101 tggctagccc caggggccag agtattaaga gccgacaagc ctcagtaggt gacaggaggt
     8161 ccccaagcac cgacatcaca gccgagggca gtcccaccaa agtgcagaag agctggagct
     8221 tcaacgaccg aacccgcttc cggccctcgc tgcgcctcaa aagttctcag ccaaaaccag
     8281 tgatagatgc tgacacagcc cttggcactg atgatgtata tgatgaaaaa ggatgccagt
     8341 gtgatgtatc agtggaagac ctcaccccac cacttaaaac tgtcattcga gctatcagaa
     8401 ttatgaaatt tcatgttgca aaacggaagt ttaaggaaac attacgtcca tatgatgtaa
     8461 aagatgtcat tgaacaatat tctgctggtc atctggacat gttgtgtaga attaaaagcc
     8521 ttcaaacacg tgttgatcaa attcttggaa aagggcaaat cacatcagat aagaagagcc
     8581 gagagaaaat aacagcagaa catgagacca cagacgatct cagtatgctc ggtcgggtgg
     8641 tcaaggttga aaaacaggta cagtccatag aatccaagct ggactgccta ctagacatct
     8701 atcaacaggt ccttcggaaa ggctctgcct cagccctcgc tttggcttca ttccagatcc
     8761 caccttttga atgtgaacag acatctgact atcaaagccc tgtggatagc aaagatcttt
     8821 cgggttccgc acaaaacagt ggctgcttat ccagatcaac tagtgccaac atctcgagag
     8881 gcctgcagtt cattctgacg ccaaatgagt tcagtgccca gactttctac gcgcttagcc
     8941 ctactatgca cagtcaagca acacaggtgc caattagtca aagcgatggc tcagcagtgg
     9001 cagccaccaa caccattgca aaccaaataa atacggcacc caagccagca gccccaacaa
     9061 ctttacagat cccacctcct ctcccagcca tcaagcatct gcccaggcca gaaactctgc
     9121 accctaaccc tgcaggctta caggaaagca tttctgacgt caccacctgc cttgttgcct
     9181 ccaaggaaaa tgttcaggtt gcacagtcaa atctcaccaa ggaccgttct atgaggaaaa
     9241 gctttgacat gggaggagaa actctgttgt ctgtctgtcc catggtgccg aaggacttgg
     9301 gcaaatcttt gtctgtgcaa aacctgatca ggtcgaccga ggaactgaat atacaacttt
     9361 cagggagtga gtcaagtggc tccagaggca gccaagattt ttaccccaaa tggagggaat
     9421 ccaaattgtt tataactgat gaagaggtgg gtcccgaaga gacagagaca gacacttttg
     9481 atgccgcacc gcagcctgcc agggaagctg cctttgcatc agactctcta aggactggaa
     9541 ggtcacgatc atctcagagc atttgtaagg caggagaaag tacagatgcc ctcagcttgc
     9601 ctcatgtcaa actgaaataa gttcttcatt ttctttccag gcatagcagt tctttagcca
     9661 tacatatcat tgcatgaact atttcgaaag cccttctaaa aagttgaaat tgcaagaatc
     9721 gggaagaaca tgaaaggcag tttataagcc cgttaccttt taattgcatg aaaatgcatg
     9781 tttagggatg gctaaaattc caaggtgcat cgacattaac ccactcattt agtaatgtac
     9841 cttgagttaa aaagcctgag aaaccaaaca cagctaatgc tatggggtgt atgaatatgt
     9901 caagtttagg tcatttagaa gatttgacac tgtattttga aattatggga gtaaacacct
     9961 tcaaatttca ggcatttctg ctttgtgact aaatacaaac tacattttca agattaggcc
    10021 ataatgtata tttaaacaca atggctatca acagctgcta ataaggtatc aactaaagca
    10081 gaattgggga ataatagaaa tggctgctta tttcaagata tatttgccaa cccattccta
    10141 ttcagtcatt ttattattaa tgtaatttga atgtcaattt gtgtgctttt ggtgatttag
    10201 cgcccgggat ccgcccctct ccctcc
//
