LOCUS       sequence-404051-        9712 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9712)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9712)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1809
FEATURES             Location/Qualifiers
     source          1..9712
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1190..1199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    2650..3238
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2703..2723)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3121..3132)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3310..3543
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3621..3742
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3658..3677)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3712..3731
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3782..3917
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3937..3956)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3938..3956)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3966..3983)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3966..3982)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3975..3997)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4124..4579
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4211..4230)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4421..4442
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4605..4709
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4710..4778,4779..5570)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4928..4947)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5741..6329
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6230..6249
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6483..6500
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6617..6638
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6653..6658,6659..6676,6677..6683)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6691..6707
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6696..6718
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6715..6731
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6715..6731
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6750..6770
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6752..6770
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6798..7024
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7025..7205
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7252..7377
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7870..8103
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8288..8332
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8563..8773
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8585..8781
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8632..8767
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8813..9415
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8813..8832)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9309..9329
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9458..9575
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatggc
     1201 catgagtgag ctgggcactc ggaagcccag cgatggcact gtttctcatc tgctcaatgt
     1261 ggtggagagt gagcttcagg cagggaggga aaaaggcgac cctacggaga agcaacttca
     1321 gatcatcctg gaggatgcac ctctctggca gagattcaag gaagtcacta atgagatgat
     1381 tgtgaccaag aatggcagac ggatgtttcc agtcctaaag attagtgtca cagggttgga
     1441 ccccaatgcc atgtactccc tcctgctgga ctttgtccct acggacagtc accgctggaa
     1501 gtacgtcaac ggggaatggg tgcccgctgg caagccagag gtctccagcc acagctgcgt
     1561 ctacattcac ccggactccc ccaactttgg ggcccactgg atgaaagctc ccatctcctt
     1621 cagcaaagtg aagctgacca acaagctcaa tggaggcggg cagataatgt tgaattctct
     1681 gcataaatat gaaccccagg ttcacatagt gcgtgttgga agtgcccatc gaatggtaac
     1741 aaactgctcc ttccctgaaa cccagttcat agccgtgact gcctatcaga atgaggagat
     1801 aacggctctc aaaatcaagt acaatccttt tgccaaagcc ttcttggatg ccaaggaaag
     1861 aaatcaccta agagacgtac cggaggctat ctctgagagc cagcatgtga cctattctca
     1921 cttgggaggc tggatctttt ccaatccaga tggagtgtgc acagcaggaa actccaatta
     1981 ccagtatgcc gctcctctgc ctctgcctgc tccccacacc caccatggct gtgagcacta
     2041 ttcgggtctc cgaggacacc ggcaggctcc ctacccttct gcgtacatgc acagaaacca
     2101 ttctccctca gtgaatttga tagaaagctc aagcaataat ctgcaagttt tctcgggacc
     2161 tgacagctgg acttccttat cctccacacc ccatgccagc atcctgtctg taccccacac
     2221 caacggacca atcaatccag ggcccagccc ctacccgtgc ctgtggacca tcagcaatgg
     2281 tgccggaggc cccagtgggc caggcccgga ggtgcacgcc agcaccccag gagcatttct
     2341 cctcggaaac ccagctgtga cttcaccccc ttctgtgctc tccacccaag cacccacttc
     2401 ggctggtgtg gaggttctgg gggagccctc gctaaccagc attgctgtgt ccacctggac
     2461 agcagtggcc tcgcatccct tcgcgggctg gggtggccca ggagcgggtg ggcaccattc
     2521 tccttcctca ctggatggtt agtaatgaac tagtccgtaa cttgaaagta tttcgatttc
     2581 ttggctttat atatcttgtg gaaaggacga ttcgtttctg gataaagaga aactacgcgt
     2641 taagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     2701 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     2761 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     2821 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     2881 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     2941 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     3001 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
     3061 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     3121 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     3181 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcgt
     3241 cgactttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     3301 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta
     3361 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     3421 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     3481 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     3541 gcagtacgta tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     3601 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
     3661 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     3721 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc
     3781 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     3841 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     3901 aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg
     3961 cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     4021 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     4081 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc
     4141 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     4201 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     4261 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     4321 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     4381 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     4441 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     4501 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     4561 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     4621 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     4681 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     4741 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     4801 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     4861 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     4921 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     4981 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     5041 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     5101 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     5161 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     5221 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     5281 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     5341 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     5401 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     5461 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     5521 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     5581 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     5641 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     5701 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     5761 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     5821 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     5881 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     5941 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     6001 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     6061 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     6121 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     6181 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     6241 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     6301 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     6361 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     6421 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     6481 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     6541 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     6601 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     6661 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     6721 acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc
     6781 tggagctgca agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac
     6841 gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga
     6901 agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg
     6961 acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat
     7021 aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     7081 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     7141 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     7201 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
     7261 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
     7321 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
     7381 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
     7441 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
     7501 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
     7561 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
     7621 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
     7681 gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg
     7741 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
     7801 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
     7861 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
     7921 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
     7981 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
     8041 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
     8101 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
     8161 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
     8221 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
     8281 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
     8341 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
     8401 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
     8461 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccatgc attgcatctc
     8521 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     8581 agcatgcgtc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     8641 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     8701 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     8761 ggaggcctag gcttttgcaa aaagctttct agaggtacca ccggagctta ccatgaccga
     8821 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct
     8881 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat
     8941 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
     9001 ggtgtgggtc gcggacgacg gcgccgccgt ggcggtctgg accacgccgg agagcgtcga
     9061 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
     9121 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
     9181 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
     9241 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
     9301 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
     9361 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcca gttaacgaat
     9421 tctagatctt gagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
     9481 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
     9541 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
     9601 atccactttg gcgccggctc gaggcctgca ggtgcaaaga tggataaagt tttaaacaga
     9661 gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa tt
//
