LOCUS       sequence-404572-       10498 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10498)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10498)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1021
FEATURES             Location/Qualifiers
     source          1..10498
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    6355..6943
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6408..6428)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6826..6837)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7015..7248
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7326..7447
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7363..7382)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7417..7436
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7487..7622
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7642..7661)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7643..7661)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7671..7688)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7671..7687)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7680..7702)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7829..8284
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7916..7935)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8126..8147
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8310..8414
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8415..8483,8484..9275)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8633..8652)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9446..10034
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9935..9954
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10188..10205
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10322..10343
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10358..10363,10364..10381,10382..10388)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10396..10412
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10401..10423
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10420..10436
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10420..10436
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10455..10475
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10457..10475
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggac ctcccggcgc tgctccccgc
     4141 cccgactgcg cgcggagggc aacatggcgg cggccccggc ccgctccgcc gagccccagc
     4201 gccgctcggc gcgagccccg cgcgccgccg cctgctactg gtgcggggcc ctgaagatgg
     4261 cgggcccggg gcgcggcccg gggaggcctc cgggccaagc ccgccgcccg ccgaggacga
     4321 cagcgacggc gactctttct tggtgctgct ggaagtgccg cacggcggcg ctgccgccga
     4381 ggctgccgga tcacaggagg ccgagcctgg ctcccgtgtc aacctggcga gccgccccga
     4441 gcagggcccc agcggcccgg ccgccccccc cggccctggc gtagccccgg cgggcgccgt
     4501 caccatcagc agccaggacc tgctggtgcg tctcgaccgc ggcgtcctcg cgctgtctgc
     4561 gccgcccggc cccgcaaccg cgggcgccgc cgctccccgc cgcgcgcccc aggcctccgg
     4621 ccccagcacg cccggctacc gctgccccga gccgcagtgc gcgctggcct tcgccaagaa
     4681 gcaccagctc aaggtgcacc tgctcacgca cggcggcggt cagggccggc ggcccttcaa
     4741 gtgcccactg gagggctgtg gttgggcctt cacaacgtcc tacaagctca agcggcacct
     4801 gcagtcgcac gacaagctgc ggcccttcgg ctgtccagtg ggcggctgtg gcaagaagtt
     4861 cactacggtc tataacctca aggcgcacat gaagggccac gagcaggaga gcctgttcaa
     4921 gtgcgaggtg tgcgccgagc gcttccccac gcacgccaag ctcagctccc accagcgcag
     4981 ccacttcgag cccgagcgcc cttacaagtg tgactttccc ggctgtgaga agacatttat
     5041 cacagtgagt gccctgtttt cccataaccg agcccacttc agggaacaag agctcttttc
     5101 ctgctccttt cctgggtgca gcaagcagta tgataaagcc tgtcggctga aaattcacct
     5161 gcggagccat acaggtgaaa gaccatttat ttgtgactct gacagctgtg gctggacctt
     5221 caccagcatg tccaaacttc taaggcacag aaggaaacat gacgatgacc ggaggtttac
     5281 ctgccctgtc gagggctgtg ggaaatcatt caccagagca gagcatctga aaggccacag
     5341 cataacccac ctaggcacaa agccgttcga gtgtcctgtg gaaggatgtt gcgcgaggtt
     5401 ctccgctcgt agcagtctgt acattcactc taagaaacac gtgcaggatg tgggtgctcc
     5461 gaaaagccgt tgcccagttt ctacctgcaa cagactcttc acctccaagc acagcatgaa
     5521 ggcgcacatg gtcagacagc acagccggcg ccaagatctc ttacctcagc tagaagctcc
     5581 gagttctctt actcccagca gtgaactcag cagcccaggc caaagtgagc tcactaacat
     5641 ggatcttgct gcactcttct ctgacacacc tgccaatgct agtggttctg caggtgggtc
     5701 ggatgaggct ctgaactccg gaatcctgac tattgacgtc acttctgtga gctcctctct
     5761 gggagggaac ctccctgcta ataatagctc cctagggccg atggaacccc tggtcctggt
     5821 ggcccacagt gatattcccc caagcctgga cagccctctg gttctcggga cagcagccac
     5881 ggttctgcag cagggcagct tcagtgtgga tgacgtgcag actgtgagtg caggagcatt
     5941 aggctgtctg gtggctctgc ccatgaagaa cttgagtgac gacccactgg ctttgacctc
     6001 caatagtaac ttagcagcac atatcaccac accgacctct tcgagcaccc cccgagaaaa
     6061 tgccagtgtc ccggaactgc tggctccaat caaggtggag ccggactcgc cttctcgccc
     6121 aggagcagtt gggcagcagg aaggaagcca tgggctgccc cagtccacgt tgcccagtcc
     6181 agcagagcag cacggtgccc aggacacaga gctcagtgca ggcactggca acttctattt
     6241 ggtatagtaa tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc
     6301 ttgtggaaag gacgacgctt gctcacatcg gaagaaccta cgcgttaagt cgacaatcaa
     6361 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     6421 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     6481 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     6541 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     6601 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     6661 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     6721 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     6781 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     6841 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     6901 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcgtcgact ttaagaccaa
     6961 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     7021 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
     7081 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     7141 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     7201 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt acgtatagta
     7261 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     7321 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     7381 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     7441 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac
     7501 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     7561 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     7621 cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc
     7681 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     7741 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     7801 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
     7861 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     7921 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     7981 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     8041 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     8101 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     8161 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     8221 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     8281 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     8341 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     8401 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     8461 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     8521 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     8581 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     8641 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     8701 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     8761 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     8821 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     8881 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     8941 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     9001 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     9061 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     9121 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     9181 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     9241 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     9301 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     9361 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     9421 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     9481 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     9541 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     9601 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     9661 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     9721 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     9781 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     9841 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     9901 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     9961 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
    10021 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
    10081 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
    10141 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
    10201 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
    10261 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
    10321 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
    10381 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
    10441 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagc
//
