LOCUS       sequence-404719-       10125 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of adenine-to-cytosine base editor ACBE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10125)
  AUTHORS   Chen L, Hong M, Luan C, Gao H, Ru G, Guo X, Zhang D, Zhang S, Li C, 
            Wu J, Randolph PB, Sousa AA, Qu C, Zhu Y, Guan Y, Wang L, Liu M, 
            Feng B, Song G, Liu DR, Li D
  TITLE     Adenine transversion editors enable precise, efficient A*T-to-C*G 
            base editing in mammalian cells and embryos.
  JOURNAL   Nat Biotechnol. 2023 Jun 15. doi: 10.1038/s41587-023-01821-9.
  PUBMED    37322276
REFERENCE   2  (bases 1 to 10125)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2023 Jun 15. doi: 10.1038/s41587-023-01821-9."
COMMENT     Sequence Label: ACBE
FEATURES             Location/Qualifiers
     source          1..10125
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        132..335
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     286..306
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     377..396
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        377..395
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             445..465
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             466..2181
                     /codon_start=1
                     /product="N-terminal portion of Streptococcus pyogenes Cas9
                     (Zetsche et al., 2015)"
                     /label=Cas9(N)
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIE"
     CDS             6295..6315
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6325..6381
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(6382..6384,6385..6387,6388..7098)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6382..6384,6385..6387,6388..7098)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6688..6707)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7035..7056
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             7111..7131
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7140..7157
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7180..7197)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7186..7410
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7481..7497)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7481..7497)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7494..7516)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7505..7521
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7529..7535,7536..7553,7554..7559))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7574..7595
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7712..7729)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7883..8471)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7963..7982)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8642..9433,9434..9502))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9265..9284
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9503..9607)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9686..9705)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(9877..10125,1..131)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gaaacggaca
      421 gccgacggaa gcgagttcga gtcaccaaag aagaagcgga aagtcgacaa gaagtacagc
      481 atcggcctgg ccatcggcac caactctgtg ggctgggccg tgatcaccga cgagtacaag
      541 gtgcccagca agaaattcaa ggtgctgggc aacaccgacc ggcacagcat caagaagaac
      601 ctgatcggag ccctgctgtt cgacagcggc gaaacagccg aggccacccg gctgaagaga
      661 accgccagaa gaagatacac cagacggaag aaccggatct gctatctgca agagatcttc
      721 agcaacgaga tggccaaggt ggacgacagc ttcttccaca gactggaaga gtccttcctg
      781 gtggaagagg ataagaagca cgagcggcac cccatcttcg gcaacatcgt ggacgaggtg
      841 gcctaccacg agaagtaccc caccatctac cacctgagaa agaaactggt ggacagcacc
      901 gacaaggccg acctgcggct gatctatctg gccctggccc acatgatcaa gttccggggc
      961 cacttcctga tcgagggcga cctgaacccc gacaacagcg acgtggacaa gctgttcatc
     1021 cagctggtgc agacctacaa ccagctgttc gaggaaaacc ccatcaacgc cagcggcgtg
     1081 gacgccaagg ccatcctgtc tgccagactg agcaagagca gacggctgga aaatctgatc
     1141 gcccagctgc ccggcgagaa gaagaatggc ctgttcggaa acctgattgc cctgagcctg
     1201 ggcctgaccc ccaacttcaa gagcaacttc gacctggccg aggatgccaa actgcagctg
     1261 agcaaggaca cctacgacga cgacctggac aacctgctgg cccagatcgg cgaccagtac
     1321 gccgacctgt ttctggccgc caagaacctg tccgacgcca tcctgctgag cgacatcctg
     1381 agagtgaaca ccgagatcac caaggccccc ctgagcgcct ctatgatcaa gagatacgac
     1441 gagcaccacc aggacctgac cctgctgaaa gctctcgtgc ggcagcagct gcctgagaag
     1501 tacaaagaga ttttcttcga ccagagcaag aacggctacg ccggctacat tgacggcgga
     1561 gccagccagg aagagttcta caagttcatc aagcccatcc tggaaaagat ggacggcacc
     1621 gaggaactgc tcgtgaagct gaacagagag gacctgctgc ggaagcagcg gaccttcgac
     1681 aacggcagca tcccccacca gatccacctg ggagagctgc acgccattct gcggcggcag
     1741 gaagattttt acccattcct gaaggacaac cgggaaaaga tcgagaagat cctgaccttc
     1801 cgcatcccct actacgtggg ccctctggcc aggggaaaca gcagattcgc ctggatgacc
     1861 agaaagagcg aggaaaccat caccccctgg aacttcgagg aagtggtgga caagggcgct
     1921 tccgcccaga gcttcatcga gcggatgacc aacttcgata agaacctgcc caacgagaag
     1981 gtgctgccca agcacagcct gctgtacgag tacttcaccg tgtataacga gctgaccaaa
     2041 gtgaaatacg tgaccgaggg aatgagaaag cccgccttcc tgagcggcga gcagaaaaag
     2101 gccatcgtgg acctgctgtt caagaccaac cggaaagtga ccgtgaagca gctgaaagag
     2161 gactacttca agaaaatcga gtgcttcgac tccgtggaaa tctccggcgt ggaagatcgg
     2221 ttcaacgcct ccctgggcac ataccacgat ctgctgaaaa ttatcaagga caaggacttc
     2281 ctggacaatg aggaaaacga ggacattctg gaagatatcg tgctgaccct gacactgttt
     2341 gaggacagag agatgatcga ggaacggctg aaaacctatg cccacctgtt cgacgacaaa
     2401 gtgatgaagc agctgaagcg gcggagatac accggctggg gcaggctgag ccggaagctg
     2461 atcaacggca tccgggacaa gcagtccggc aagacaatcc tggatttcct gaagtccgac
     2521 ggcttcgcca acagaaactt catgcagctg atccacgacg acagcctgac ctttaaagag
     2581 gacatccaga aagcccaggt gtccggccag ggcgatagcc tgcacgagca cattgccaat
     2641 ctggccggca gccccgccat taagaagggc atcctgcaga cagtgaaggt ggtggacgag
     2701 ctcgtgaaag tgatgggccg gcacaagccc gagaacatcg tgatcgaaat ggccagagag
     2761 aaccagacca cccagaaggg acagaagaac agccgcgaga gaatgaagcg gatcgaagag
     2821 ggcatcaaag agctgggcag ccagatcctg aaagaacacc ccgtggaaaa cacccagctg
     2881 cagaacgaga agctgtacct gtactacctg cagaatgggc gggatatgta cgtggaccag
     2941 gaactggaca tcaaccggct gtccgactac gatgtggacc atatcgtgcc tcagagcttt
     3001 ctgaaggacg actccatcga caacaaggtg ctgaccagaa gcgacaagaa ccggggcaag
     3061 agcgacaacg tgccctccga agaggtcgtg aagaagatga agaactactg gcggcagctg
     3121 ctgaacgcca agctgattac ccagagaaag ttcgacaatc tgaccaaggc cgagagaggc
     3181 ggcctgagcg aactggataa ggccggcttc atcaagagac agctggtgga aacccggcag
     3241 atcacaaagc acgtggcaca gatcctggac tcccggatga acactaagta cgacgagaat
     3301 gacaagctga tccgggaagt gaaagtgatc accctgaagt ccaagctggt gtccgatttc
     3361 cggaaggatt tccagtttta caaagtgcgc gagatcaaca actaccacca cgcccacgac
     3421 gcctacctga acgccgtcgt gggaaccgcc ctgatcaaaa agtaccctaa gctggaaagc
     3481 gagttcgtgt acggcgacta caaggtgtac gacgtgcgga agatgatcgc caagagcgag
     3541 caggaaatcg gcaaggctac cgccaagtac ttcttctaca gcaacatcat gaactttttc
     3601 aagaccgaga ttaccctggc caacggcgag atccggaagc ggcctctgat cgagacaaac
     3661 ggcgaaaccg gggagatcgt gtgggataag ggccgggatt ttgccaccgt gcggaaagtg
     3721 ctgagcatgc cccaagtgaa tatcgtgaaa aagaccgagg tgcagacagg cggcttcagc
     3781 aaagagtcta tcctgcccaa gaggaacagc gataagctga tcgccagaaa gaaggactgg
     3841 gaccctaaga agtacggcgg cttcgacagc cccaccgtgg cctattctgt gctggtggtg
     3901 gccaaagtgg aaaagggcaa gtccaagaaa ctgaagagtg tgaaagagct gctggggatc
     3961 accatcatgg aaagaagcag cttcgagaag aatcccatcg actttctgga agccaagggc
     4021 tacaaagaag tgaaaaagga cctgatcatc aagctgccta agtactccct gttcgagctg
     4081 gaaaacggcc ggaagagaat gctggcctct gccggcgaac tgcagaaggg aaacgaactg
     4141 gccctgccct ccaaatatgt gaacttcctg tacctggcca gccactatga gaagctgaag
     4201 ggctccagcg gaggatctag cggaggatca agcggaagcg agactcctgg aaccagcgaa
     4261 agcgcaaccc cagaaagcag cggaggaagt agcggaggaa gcccggcgcg gggcggctca
     4321 gcccgtccag ggagaggcgc actgaagccc gtgagcgtga ccctgctgcc cgacaccgag
     4381 cagcccccct tcttaggcag agcgcgtaga cctggcaatg ctagagcggg gagcctggtg
     4441 acaggatacc acgaggtggg ccagatgccc gcccccctga gcagaaagat cggccagaag
     4501 aagcagagac tggccgatag cgagcagcag cagaccccca aggagagact gctgagcacc
     4561 cccggcctga gaagaagcat ctacttcagc agccccgagg accacagcgg cagactgggc
     4621 ccagagtttt tcgaccagcc cgccgtgacc ctggccagag ccttcctggg ccaggtgctg
     4681 gtgagaagac tggccgacgg caccgagctg agaggcagaa tcgtggagac cgaggcctac
     4741 ttgggacccg aggacgaggc cgcccacagc agaggaggca gacagacccc cgagaacaga
     4801 ggcatgttca tgaagcccgg caccctgtac gtgttcctga tctacggcat gtacttctgc
     4861 ttgaacgtga gctctcaggg cgccggcgcc tgcgtactcc tcagagccct ggagcccctg
     4921 gagggcctgg agaccatgag acagctgaga aacagcctga gaaagagcac cgtgggcaga
     4981 agcctgaagg acagagagct gtgcagcggc cccagcaagc tgtgccaggc cctggccatc
     5041 gacaagagct tcgaccagag agacttggcg caagatgacg ccgtgtggct ggaacacggg
     5101 cccttggaga gcagcagccc agccgtagtg gtggcggccg ccagaatcgg catcggccac
     5161 gccggcgagt ggacccagaa gcccctgaga ttctacgtgc agggcagccc ctgggtgagc
     5221 gtggtggaca gagtggccga gcagatggac cagccccagc agaccgcctg cagcgagggc
     5281 ctgctgatcg tgcagaagtc tgaggtggag ttttcccacg agtactggat gagacatgcc
     5341 ctgaccctgg ccaagagggc acgggatgag agggaggtgc ctgtgggagc cgtgctggtg
     5401 ctgaacaata gagtgatcgg cgagggctgg aacagagcca tcggcctgca cgacccaaca
     5461 gcccatgccg aaattatggc cctgagacag ggcggcctgg tcatgcagaa ctacagactg
     5521 attgacgcca ccctgtacgt gacattcgag ccttgcgtga tgtgcgccgg cgccatgatc
     5581 cactctagga tcggccgcgt ggtgtttggc gtgaggaact caaaaagagg cgccgcaggc
     5641 tccctgatga acgtgctgaa ctaccccggc atgaatcacc gcgtcgaaat taccgaggga
     5701 atcctggcag atgaatgtgc cgccctgctg tgcgatttct atcggatgcc tagacaggtg
     5761 ttcaatgctc agaagaaggc ccagagctcc atcaactccg gaggatctag cggaggctcc
     5821 tctggctctg agacacctgg cacaagcgag agcgcaacac ctgaaagcag cgggggcagc
     5881 agcggggggt cacccgagga taatgagcag aaacagctgt ttgtggaaca gcacaagcac
     5941 tacctggacg agatcatcga gcagatcagc gagttctcca agagagtgat cctggccgac
     6001 gctaatctgg acaaagtgct gtccgcctac aacaagcacc gggataagcc catcagagag
     6061 caggccgaga atatcatcca cctgtttacc ctgaccaatc tgggagcccc tgccgccttc
     6121 aagtactttg acaccaccat cgaccggaag aggtacacca gcaccaaaga ggtgctggac
     6181 gccaccctga tccaccagag catcaccggc ctgtacgaga cacggatcga cctgtctcag
     6241 ctgggaggtg actctggcgg ctcaaaaaga accgccgacg gcagcgaatt cgagcccaag
     6301 aagaagagga aagtcggaag cggagctact aacttcagcc tgctgaagca ggctggagac
     6361 gtggaggaga accctggacc tatggtgagc aagggcgagg agctgttcac cggggtggtg
     6421 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     6481 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     6541 ctgcccgtgc cctggcccac cctcgtgacc accctgacct atggagtgca gtgcttcagc
     6601 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     6661 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     6721 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     6781 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     6841 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
     6901 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     6961 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
     7021 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     7081 atggacgagc tgtacaagtc tggtggttct cccaagaaga agaggaaagt ctaaccggtc
     7141 atcatcacca tcaccattga gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt
     7201 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     7261 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     7321 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     7381 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct
     7441 cgataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     7501 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     7561 cctagggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     7621 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     7681 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     7741 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     7801 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     7861 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     7921 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     7981 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     8041 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     8101 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     8161 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     8221 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     8281 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     8341 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     8401 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     8461 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacactcag tggaacgaaa
     8521 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     8581 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     8641 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     8701 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     8761 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     8821 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     8881 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     8941 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     9001 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     9061 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     9121 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     9181 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     9241 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     9301 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     9361 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     9421 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     9481 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     9541 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     9601 ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga gatcgatctc
     9661 ccgatcccct agggtcgact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
     9721 atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta
     9781 caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg
     9841 cgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt
     9901 aatagtaatc aattacgggg tcattagttc atagcccata tattgagttc cgcgttacat
     9961 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
    10021 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
    10081 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatc
//
