LOCUS       sequence-404813-        3291 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  16OMT promoter and native 5'UTR (approximately 1 kb upstream of 
            start codon) from C. roseus in a MoClo compatible L0 plasmid..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Cole-Osborn LF, McCallan SA, Prifti O, Abu R, Sjoelund V, 
            Lee-Parsons CWT
  TITLE     The role of the Golden2-like (GLK) transcription factor in 
            regulating terpenoid indole alkaloid biosynthesis in Catharanthus 
            roseus.
  JOURNAL   Plant Cell Rep. 2024 May 14;43(6):141. doi: 
            10.1007/s00299-024-03208-9.
  PUBMED    38743349
REFERENCE   2  (bases 1 to 3291)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell 
            Rep."; date: "2024-05-14"; volume: "43(6):141. doi"; pages: " 
            10.1007/s00299-024-03208-9"
COMMENT     Sequence Label: pUN1213 - pL0_p16OMT (pro + 5U)
FEATURES             Location/Qualifiers
     source          1..3291
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1089..1107)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1175..1279
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1501..2292
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2463..3051
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2952..2971
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3205..3222
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggtctcagga gttatatttg ttgctggcta tatatataat agtgaaccca taaagagcac
       61 atcatttcac agtgagaaat ccccccaaac caaacaagga aaaactatat tatactcttc
      121 ttgttaccat ttttctttta ataaatgtat aatataatat atattattgt tattttattt
      181 gacgagttct gaataaatag atatgagtaa ttaatacttt gtccatactt atttaataaa
      241 aaataattaa atataagtat gaatataagt ctcaatataa aaattaaatc ctatccacta
      301 catataaata gggtcatttg caaccctact catgaactat tgtataccat gttatatgtt
      361 tcttactttc ttatggagtt tatttactga ggaaaaaaat atatcatgac aagttcgaag
      421 tcatattttt tgtatataag aacacgagac atgtgtaact cagttgattt taaaattaaa
      481 gaattggtaa aaaaaaaaat gagaggttag attctttttg ttgataacat cagagaatag
      541 atgattgcat gaataatgta ggtggttgca attactttct caaccaactg tccaactctc
      601 attttctttc ctttactttt tttaacaacc caatgttctt tgaaacgtct taaaagattt
      661 aagacaaatt aatgatttga aatactttaa tctatggaca aaataagaca aaaatctttg
      721 aatacattaa ttttgatatg ataaatagaa aacacatttt ttttataaat ttgttaagaa
      781 tttcttccta agatttatag tatcaataaa tgttttatgg attgctcctg ttgaccagat
      841 ttccaaattt tacaaaaccc aataaaaata atttggagag atgttttttt gagcaataat
      901 gacatatagt aaaagtgtgc attagattaa ataataggcg cccaaatttg acgaagagtt
      961 cgtgaccatc tctctatata tagcatacca taccatgtct cttctcaaga gagcacaaac
     1021 ttaccacccc caacccccag aaactcgcca atgtgagacc acgaagtggc tcttcagtgg
     1081 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     1141 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     1201 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     1261 atattgaaaa aggaagagta tgcgctcacg caactggtcc agaaccttga ccgaacgcag
     1321 cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt ttttggggta
     1381 cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg
     1441 ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc
     1501 atgagggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc
     1561 gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc
     1621 ggcctgaagc cacacagcga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa
     1681 acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc
     1741 gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt
     1801 tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt
     1861 atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa
     1921 catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag
     1981 gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct
     2041 ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc
     2101 aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat
     2161 cagcccgtca tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc
     2221 tcgcgcgcag atcagttgga agaatttgtc cattacgtaa aaggcgagat caccaaggta
     2281 gtcggcaaat aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     2341 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     2401 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     2461 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     2521 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     2581 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     2641 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     2701 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     2761 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     2821 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     2881 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     2941 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     3001 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     3061 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     3121 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     3181 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     3241 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatcactctg t
//
