LOCUS       sequence-404823-        2607 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo lvl0 3'UTR+Terminator part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Yang EJY, Nemhauser JL
  TITLE     Building a pipeline to identify and engineer constitutive and 
            repressible promoters.
  JOURNAL   Quant Plant Biol. 2023 Oct 19;4:e12. doi: 10.1017/qpb.2023.10. 
            eCollection 2023.
  PUBMED    37901686
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Quant Plant
            Biol."; date: "2023-10-19"; volume: "4:e12. doi"; pages: " 
            10.1017/qpb.2023.10. eCollection 2023"
COMMENT     Sequence Label: pICH41276(3Uter)-tAT5G60160
FEATURES             Location/Qualifiers
     source          1..2607
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      295..883
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     784..803
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1037..1054
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1528..1546)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1614..1718
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1940..2607,1..124)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc
       61 agatcagttg gaagaatttg tccattacgt aaaaggcgag atcaccaagg tagtcggcaa
      121 ataactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
      181 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
      241 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
      301 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
      361 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
      421 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
      481 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
      541 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
      601 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
      661 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
      721 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
      781 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
      841 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
      901 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
      961 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     1021 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     1081 accgcctctc cccgcgcgtt ggccgattca ttaatcactc tgtggtctca gcttgagatt
     1141 cttaaatctt actgctgttt aagttggaac tgaaaagatc ctttgttagg aaaaactaca
     1201 ttaataaaag gatttatagc catgttatgt gttgcctgcg gttaccatca attcaccctc
     1261 ccgaatattc atcaacttat tacttaagcg attcatttaa ttgagtggtt tatctcaatt
     1321 atcttgaaat gaagaagatc aaaagacata tatatgatac ttttgttaga gcttcgaaaa
     1381 agacaaagac agaaagataa caactagcaa tgcggtttca ggcaaatccg tcatggtcca
     1441 tgatgatgaa ctagcctgat ctggctctta acaaatctca attttggtcg cttgagacca
     1501 cgaagtggct cttcagtgga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     1561 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     1621 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     1681 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gcgctcacgc aactggtcca
     1741 gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg
     1801 actgtttttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc
     1861 cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc
     1921 taaaacaaag ttaaacatca tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc
     1981 agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta
     2041 cggctccgca gtggatggcg gcctgaagcc acacagcgat attgatttgc tggttacggt
     2101 gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc
     2161 ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga
     2221 cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg
     2281 caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt
     2341 gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt
     2401 tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa
     2461 ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg
     2521 gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga
     2581 gcgcctgccg gcccagtatc agcccgt
//
