LOCUS       sequence-404827-        3335 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo lvl0 Promoter + 5'UTR part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   Yang EJY, Nemhauser JL
  TITLE     Building a pipeline to identify and engineer constitutive and 
            repressible promoters.
  JOURNAL   Quant Plant Biol. 2023 Oct 19;4:e12. doi: 10.1017/qpb.2023.10. 
            eCollection 2023.
  PUBMED    37901686
REFERENCE   2  (bases 1 to 3335)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Quant Plant
            Biol."; date: "2023-10-19"; volume: "4:e12. doi"; pages: " 
            10.1017/qpb.2023.10. eCollection 2023"
COMMENT     Sequence Label: pICH41295(pro5U)-pAT4G13780
FEATURES             Location/Qualifiers
     source          1..3335
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      281..869
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     770..789
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1023..1040
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2242..2260)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2328..2432
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2654..3335,1..110)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 gacaggctta tcttggacaa gaagaagatc gcttggcctc gcgcgcagat cagttggaag
       61 aatttgtcca ttacgtaaaa ggcgagatca ccaaggtagt cggcaaataa ctgtcagacc
      121 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
      181 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
      241 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
      301 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
      361 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
      421 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
      481 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
      541 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
      601 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
      661 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
      721 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
      781 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
      841 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
      901 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
      961 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     1021 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     1081 cgcgttggcc gattcattaa tcactctgtg gtctcaggag aatcatgaga ttttttttaa
     1141 aaaagttttt tttaaacttt ggtgttatta tttaaaaatg ttactaaaat catcgttctc
     1201 tgtgtcgaaa tctttgtgtg aagtttaatc attaaaaaaa aatctactag agatgaagtt
     1261 ttaacgatga agaaaatgat atgatttatc atatttatag agatttaaca taaatattcc
     1321 tataattttg ttttatacga aataaattat acaaagaatc ttaccactaa cgagtgcctt
     1381 taaacacttc tttgacatgt tgtcaaatgt tagcttgtga ttatagacat gattactcta
     1441 ttgcaatttg taggtaaata attaaacaat taaatttgcc tccacatttt tccctctctg
     1501 acaagtatac cttggaagat tctgcctctt agtcatatga tagatattct tcaaccgttg
     1561 tttagagaat tggtacataa ctctcttatt ttaaccatag tgttatccat atgtagcatt
     1621 tgaaatatat gttgtgaaat gatataataa cagaaagatg aagaagtcca acaatatgaa
     1681 aaaagttcgc ttcaagtttg ggctgataga cgtggtaaat ctgttttgtt taacataaaa
     1741 gatagaccta gtcacctagc aaagagtttc tataggacgt ggtcatgttg taaaacaaaa
     1801 gaaaatgatt taggagaaag tttccatgta atataggacg tggtcatgtt gtattttttt
     1861 tttttgttaa tggtaatttc ctaattaata tagtcacttt actttagttt cacaaaagtt
     1921 taataattca ctaaactata gtaaagctat ttagattccg gtttaattcc atccatacca
     1981 gtaagatcaa tatcttgcaa atgcaattca gttatcaaac cggaaagtga ccaactttct
     2041 gtctggtcta aacttaaccg gatctatctg aggccgccgt tacttaggtt taccatcagc
     2101 taaagagctt ctcttttctc agcttctctg attcgtcttt ctcctctgtc tctgactctt
     2161 cgcgaaaacc ctaattagct ttgtggtctt gttgttttcg ccaatgtgag accacgaagt
     2221 ggctcttcag tggacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     2281 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     2341 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     2401 aaatgcttca ataatattga aaaaggaaga gtatgcgctc acgcaactgg tccagaacct
     2461 tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt
     2521 tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg
     2581 tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac
     2641 aaagttaaac atcatgaggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt
     2701 agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc
     2761 cgcagtggat ggcggcctga agccacacag cgatattgat ttgctggtta cggtgaccgt
     2821 aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc
     2881 ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat
     2941 cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga
     3001 cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac
     3061 aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc
     3121 ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc
     3181 gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag
     3241 cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct
     3301 gccggcccag tatcagcccg tcatacttga agcta
//
