LOCUS       sequence-404888-        3096 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo lvl0 3'UTR+Terminator part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Yang EJY, Nemhauser JL
  TITLE     Building a pipeline to identify and engineer constitutive and 
            repressible promoters.
  JOURNAL   Quant Plant Biol. 2023 Oct 19;4:e12. doi: 10.1017/qpb.2023.10. 
            eCollection 2023.
  PUBMED    37901686
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Quant Plant
            Biol."; date: "2023-10-19"; volume: "4:e12. doi"; pages: " 
            10.1017/qpb.2023.10. eCollection 2023"
COMMENT     Sequence Label: pICH41276(3Uter)-tAT1G18700
FEATURES             Location/Qualifiers
     source          1..3096
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      282..870
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     771..790
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1024..1041
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2004..2022)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2090..2194
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2416..3096,1..111)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa
       61 gaatttgtcc attacgtaaa aggcgagatc accaaggtag tcggcaaata actgtcagac
      121 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
      181 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
      241 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
      301 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
      361 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
      421 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
      481 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
      541 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
      601 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
      661 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
      721 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
      781 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
      841 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
      901 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
      961 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     1021 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     1081 gcgcgttggc cgattcatta atcactctgt ggtctcagct ttatattaag ccttaggatt
     1141 cttctgactt tctttttttt tgacatttac gttttttatt ttttcttttc tttgtgaaag
     1201 tcataggttt caattttaag ttactaaata gagtaattta cctttatcat tttgaacact
     1261 tcaaaatatt cgatcccctt ttgcttatac agtaccacat tttagatttc ctaaattgaa
     1321 agaaaaaaca caacttataa aactagcaat aatgccaata ttttccatat atgttgtatc
     1381 actctttgtt tatactttta tattatttga ttatagacac gaaagctcta cagaaacatc
     1441 tcaaatccaa aaaaaataaa atatattata tatatgcgtg tgtgtgaaaa tttttgagaa
     1501 aaatttctaa tttaaagaaa acaatcataa actaatttag aaacccagat caaaagtagg
     1561 aaatttacat aagacatatc ataggggtaa atccagaatc ggatttattt agagatgaag
     1621 tgctctattt cttgaaaaat gacaaaactt tttgggtggg ccttcatttt ttaaaacttt
     1681 ttttgataac tttattgccc atatctgttt gtcgtcaaca tttttggaaa atttctttta
     1741 aatgattttt ggtaattaaa atcttaaaaa cttgattttt ttaatttaac atattgaact
     1801 gtctatatat attcctgacc aaccgtgctg ctccatatgg gacaacaacc tctttagtgc
     1861 ggtccatgaa aggtttccag aggctatgac aacaccaacc ttatctcgtg attcagtttc
     1921 acttcaacca cagagtataa aggaaaaaga ttcagaccca ttagcgcttg agaccacgaa
     1981 gtggctcttc agtggacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     2041 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     2101 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     2161 ataaatgctt caataatatt gaaaaaggaa gagtatgcgc tcacgcaact ggtccagaac
     2221 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg
     2281 tttttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2341 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2401 acaaagttaa acatcatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2461 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2521 tccgcagtgg atggcggcct gaagccacac agcgatattg atttgctggt tacggtgacc
     2581 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2641 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2701 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2761 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2821 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2881 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2941 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     3001 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     3061 ctgccggccc agtatcagcc cgtcatactt gaagct
//
