LOCUS       sequence-404890-        3220 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo lvl0 Promoter + 5'UTR part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Yang EJY, Nemhauser JL
  TITLE     Building a pipeline to identify and engineer constitutive and 
            repressible promoters.
  JOURNAL   Quant Plant Biol. 2023 Oct 19;4:e12. doi: 10.1017/qpb.2023.10. 
            eCollection 2023.
  PUBMED    37901686
REFERENCE   2  (bases 1 to 3220)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Quant Plant
            Biol."; date: "2023-10-19"; volume: "4:e12. doi"; pages: " 
            10.1017/qpb.2023.10. eCollection 2023"
COMMENT     Sequence Label: pICH41295(pro5U)-pAT1G71900
FEATURES             Location/Qualifiers
     source          1..3220
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      301..889
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     790..809
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1043..1060
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1911..1922)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2147..2165)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2233..2337
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2559..3220,1..130)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc gcttggcctc
       61 gcgcgcagat cagttggaag aatttgtcca ttacgtaaaa ggcgagatca ccaaggtagt
      121 cggcaaataa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
      181 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
      241 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
      301 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
      361 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      421 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      481 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      541 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      601 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      661 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      721 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      781 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      841 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      901 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      961 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     1021 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     1081 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tcactctgtg gtctcaggag
     1141 caattttttt actgtttttt ttgcttcggt gaaagaaaga tccaactaga gaaaataatc
     1201 ccaaaacaaa aaatcaacat tcaaaatctg agctcttttc taaaattaaa ttcaaaacat
     1261 gaacttattc tatgtaattt tttttttttg ttttaaagac atggccaatg ggcctttcac
     1321 tcttgtcaaa cccatttgag tcgggcttta gtcataactc attttaaaga ttaaggaata
     1381 tgaggccagt tcttttgtca caatcctagt ttaagcttgt ctcacttacc aattacgtct
     1441 ctcaaaagac aaattttaaa aaatctataa tctctactct ctaacatgta tatatatatg
     1501 ataaactcgt tggctattac aacttttaaa tcacaaatgg cctgattgga aaaaaatgta
     1561 tacataaatg ataagtttgt cactgattaa tgcgagaaaa ttctaattat ttgcattaat
     1621 aaaattagcg tagttaggtt ttaaaaaggt ttatacaata cattatacat atttataaat
     1681 ttaaatgttt ttaaatttat attaaattta ttttattatt tagataaatc ttaaataaaa
     1741 agaaaccaaa tattatttgt tattttttcg tgaaatttat ctttttaata tattttttat
     1801 tttagtaaaa agaaggaatg gtggcgccgg aaactgaccg tagggtcagt ctaattactc
     1861 tctcagcgtt tctctttttg gtcctcaaat cttcaccttc aagatcttct tcgtccctcg
     1921 atctccgatt tcaaatctcg tttctccgtt caagcaacct ctctgaattc ctagtctcac
     1981 agcaggaatt agtactcggc gaagcgattt ctgcttcgat ctctacatct gaagttccga
     2041 tctgacgaaa tttagggttc gtctgggtcg aagttaaata ggaaaaagat ttcgaattgg
     2101 cttgagaaat gtgagaccac gaagtggctc ttcagtggac gaaagggcct cgtgatacgc
     2161 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     2221 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     2281 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     2341 cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg
     2401 gttttcatgg cttgttatga ctgttttttt ttggggtaca gtctatgcct cgggcatcca
     2461 agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc aacgatgtta
     2521 cgcagcaggg cagtcgccct aaaacaaagt taaacatcat gagggaagcg gtgatcgccg
     2581 aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt
     2641 tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca cacagcgata
     2701 ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga gctttgatca
     2761 acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc gctgtagaag
     2821 tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag cgcgaactgc
     2881 aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca gccacgatcg
     2941 acattgatct ggctatcttg ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc
     3001 cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag gcgctaaatg
     3061 aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga aatgtagtgc
     3121 ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg
     3181 ctgccgactg ggcaatggag cgcctgccgg cccagtatca
//
