LOCUS       sequence-404893-        2681 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo lvl0 3'UTR+Terminator part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Yang EJY, Nemhauser JL
  TITLE     Building a pipeline to identify and engineer constitutive and 
            repressible promoters.
  JOURNAL   Quant Plant Biol. 2023 Oct 19;4:e12. doi: 10.1017/qpb.2023.10. 
            eCollection 2023.
  PUBMED    37901686
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Quant Plant
            Biol."; date: "2023-10-19"; volume: "4:e12. doi"; pages: " 
            10.1017/qpb.2023.10. eCollection 2023"
COMMENT     Sequence Label: pICH41276(3Uter)-tAT2G26780
FEATURES             Location/Qualifiers
     source          1..2681
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      294..882
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     783..802
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1036..1053
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1601..1619)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1687..1791
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2013..2681,1..123)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 atacttgaag ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca
       61 gatcagttgg aagaatttgt ccattacgta aaaggcgaga tcaccaaggt agtcggcaaa
      121 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
      181 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
      241 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
      301 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
      361 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
      421 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
      481 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
      541 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
      601 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
      661 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
      721 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
      781 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
      841 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
      901 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
      961 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     1021 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     1081 ccgcctctcc ccgcgcgttg gccgattcat taatcactct gtggtctcag cttgcaagca
     1141 cagagaagat tttagtttta tgcctgaagt tgatataaat gggaattgaa tctcctactt
     1201 tgtatttgtt tacttatata caagttcaca ttgatatata tatcttctaa gtttttactt
     1261 ttcttccctg atggtaaaaa tttattcacg gcttttttag ccagtctatc tacttcttac
     1321 attcgaggtt tgtgagctga gctcggaaac ccaaatttca aaattggcca aactgcaaat
     1381 cacgatcaaa tcaccatgta tttagaagct tgaccagttc cttgtgaaga gatcgtgtgg
     1441 cttgcagtca ttgcgactac tacgaatcaa gtgtatgatt attgtagagg accactgagc
     1501 ttgggacttc tcttccaagt cgatcgacac tatagattaa ttatggtgtg atacttaggt
     1561 ccgcttgaga ccacgaagtg gctcttcagt ggacgaaagg gcctcgtgat acgcctattt
     1621 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     1681 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     1741 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgcgctca
     1801 cgcaactggt ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc
     1861 atggcttgtt atgactgttt ttttttgggg tacagtctat gcctcgggca tccaagcagc
     1921 aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc
     1981 agggcagtcg ccctaaaaca aagttaaaca tcatgaggga agcggtgatc gccgaagtat
     2041 cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg
     2101 ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagc gatattgatt
     2161 tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc
     2221 ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca
     2281 ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg
     2341 gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg
     2401 atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg
     2461 cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct
     2521 taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt
     2581 tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg
     2641 actgggcaat ggagcgcctg ccggcccagt atcagcccgt c
//
