LOCUS       sequence-404900-        3311 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo lvl0 Promoter + 5'UTR part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3311)
  AUTHORS   Yang EJY, Nemhauser JL
  TITLE     Building a pipeline to identify and engineer constitutive and 
            repressible promoters.
  JOURNAL   Quant Plant Biol. 2023 Oct 19;4:e12. doi: 10.1017/qpb.2023.10. 
            eCollection 2023.
  PUBMED    37901686
REFERENCE   2  (bases 1 to 3311)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Quant Plant
            Biol."; date: "2023-10-19"; volume: "4:e12. doi"; pages: " 
            10.1017/qpb.2023.10. eCollection 2023"
COMMENT     Sequence Label: pICH41295(pro5U)-pAT1G64990
FEATURES             Location/Qualifiers
     source          1..3311
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      282..870
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     771..790
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1024..1041
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1943..1963)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     primer_bind     complement(2219..2237)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2305..2409
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2631..3311,1..111)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa
       61 gaatttgtcc attacgtaaa aggcgagatc accaaggtag tcggcaaata actgtcagac
      121 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
      181 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
      241 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
      301 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
      361 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
      421 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
      481 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
      541 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
      601 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
      661 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
      721 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
      781 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
      841 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
      901 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
      961 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     1021 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     1081 gcgcgttggc cgattcatta atcactctgt ggtctcagga gaaatgtagg attgaaaagc
     1141 ataaaaacat tataaacaaa atagctcaaa actttttttt gttaaataat tgtgatataa
     1201 taagaaaaac gttttgtagt ttttttaatt ttgaattatt ttttccacat gcatcaaata
     1261 taacatacac atgaaaatca aatataaatg tatgataaat attgtgaatg gtaaaaataa
     1321 tagaatcacg ttatgaaaaa tggtatatta tacatcatcg attggtgtct gataagtttg
     1381 gaggaaactt tagcaataat acaggttttc tctatttatt cattagctaa taggattatt
     1441 tatacaaaaa tcataacttg attagcaagt tctttacatt tatgataata ttaaattttc
     1501 tatctaacaa taagaaaatg tacacaaata ttaatttttt attaatgata aaaaaaatat
     1561 ttatcttgat cccgtaatta taagatttat catgaactat cgatatcccg atctttccct
     1621 tcccacttcc cgcaaattta tatatggtaa cacgtgtaag attggacaag ccttctaaac
     1681 aacaatttta atgaatcgat catttttcgt gtatatatgt aaaagtgaaa gaaaaacaaa
     1741 aaaatcaatc gaccagataa gaaaacatgt cggaaaatgg agttaaaatt atcatcaaca
     1801 ctagactaga cgccaaaacc tataaacaaa aaaaaaggat agaaacacta ttgttgtgca
     1861 gtttctttca cattactccg tagctcaatg gttggttagc gtttaataac gccttcctct
     1921 ctgcgagttc gagttgagtc gcatggtggt ggtggtgatg atgaagttga tgaggttgtt
     1981 gttgattaga gtttaaacca aataaaccct aaccaaaaat aaataaggta ttcttaaata
     2041 cttttttttt aattgtttca taaatgatca aattcgaatc gaatcgtaca acagaagctc
     2101 tggtgttgaa tcgccatgag gtttttgcag atctgaggtt ttttctcgag aagtaagaga
     2161 gatctggaat ttggcgacga atgtgagacc acgaagtggc tcttcagtgg acgaaagggc
     2221 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     2281 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     2341 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     2401 aggaagagta tgcgctcacg caactggtcc agaaccttga ccgaacgcag cggtggtaac
     2461 ggcgcagtgg cggttttcat ggcttgttat gactgttttt ttttggggta cagtctatgc
     2521 ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca
     2581 gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc atgagggaag
     2641 cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc
     2701 tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc
     2761 cacacagcga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc
     2821 gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc
     2881 gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta
     2941 agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc
     3001 cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg
     3061 ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg
     3121 aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc
     3181 gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc
     3241 cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca
     3301 tacttgaagc t
//
