LOCUS       sequence-404906-        2881 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo lvl0 Promoter + 5'UTR part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Yang EJY, Nemhauser JL
  TITLE     Building a pipeline to identify and engineer constitutive and 
            repressible promoters.
  JOURNAL   Quant Plant Biol. 2023 Oct 19;4:e12. doi: 10.1017/qpb.2023.10. 
            eCollection 2023.
  PUBMED    37901686
REFERENCE   2  (bases 1 to 2881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Quant Plant
            Biol."; date: "2023-10-19"; volume: "4:e12. doi"; pages: " 
            10.1017/qpb.2023.10. eCollection 2023"
COMMENT     Sequence Label: pICH41295(pro5U)-pAT1G54390
FEATURES             Location/Qualifiers
     source          1..2881
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      280..868
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     769..788
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1022..1039
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1787..1805)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1873..1977
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2199..2881,1..109)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga
       61 atttgtccat tacgtaaaag gcgagatcac caaggtagtc ggcaaataac tgtcagacca
      121 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
      181 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
      241 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
      301 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
      361 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
      421 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
      481 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
      541 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
      601 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
      661 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
      721 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
      781 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
      841 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
      901 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
      961 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     1021 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     1081 gcgttggccg attcattaat cactctgtgg tctcaggagg gtgattgtga tgatgaccca
     1141 tctaattgct tctatttata taatttgttc tgtgcatgca gtttcttctt ggatctccta
     1201 tgtactgaat gattcgttta ttacccttaa agatttgctt taaaaattaa tttatttcac
     1261 tctttcgagt ttatatagtt tgttgtatct tgtgaaattg gtgtttcatc acatgcacat
     1321 atcttcgtct atgtggttcc aaatattctt ttgttctttt tattttacca tgcattgctt
     1381 tattacttac acataatgac aattttgctg gtcattgatt agaacatcgt tttaatatcg
     1441 aatgggatgt cgagttgaat attttgacaa cgttcgttgg ctgggtcatg aatgaagtca
     1501 gaataccatt tctttttcat taaacaatgc tatttttttc ccgcaaaaca atgctattgg
     1561 gctcattttg ggcttaaaat gttttgcgta atctcaatgt ttgtggggct tttagttttt
     1621 agcccatgta tggtttgata actttagtaa ctcgtgagaa aaaaaaaagc ttcgttctca
     1681 atcccaattc gaaatcctag ctgaagtcta tgctccataa tcaaatccag ttcatcagag
     1741 atcaaagaat gtgagaccac gaagtggctc ttcagtggac gaaagggcct cgtgatacgc
     1801 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     1861 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     1921 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     1981 cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg
     2041 gttttcatgg cttgttatga ctgttttttt ttggggtaca gtctatgcct cgggcatcca
     2101 agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc aacgatgtta
     2161 cgcagcaggg cagtcgccct aaaacaaagt taaacatcat gagggaagcg gtgatcgccg
     2221 aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt
     2281 tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca cacagcgata
     2341 ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga gctttgatca
     2401 acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc gctgtagaag
     2461 tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag cgcgaactgc
     2521 aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca gccacgatcg
     2581 acattgatct ggctatcttg ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc
     2641 cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag gcgctaaatg
     2701 aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga aatgtagtgc
     2761 ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg
     2821 ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata cttgaagcta
     2881 g
//
