LOCUS       sequence-404916-        9945 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For cytosine base editing using miniSdd6 in monocotyledon plants 
            (transient transformation).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9945)
  AUTHORS   Huang J, Lin Q, Fei H, He Z, Xu H, Li Y, Qu K, Han P, Gao Q, Li B, 
            Liu G, Zhang L, Hu J, Zhang R, Zuo E, Luo Y, Ran Y, Qiu JL, Zhao KT,
            Gao C
  TITLE     Discovery of deaminase functions by structure-based protein 
            clustering.
  JOURNAL   Cell. 2023 Jul 20;186(15):3182-3195.e14. doi: 
            10.1016/j.cell.2023.05.041. Epub 2023 Jun 27.
  PUBMED    37379837
REFERENCE   2  (bases 1 to 9945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2023.05.041"; journalName: "Cell"; date: 
            "2023-07-20- 20"; volume: "186"; issue: "15"; pages: "3182-3195.e14"
COMMENT     Sequence Label: pnCas9-miniSdd6-PBE
FEATURES             Location/Qualifiers
     source          1..9945
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2019..2039
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(2520..2543,2544..2546,2547..6620)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6621..6668
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             6690..6938
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             6951..6971
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7045..7221
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     complement(7775..7791)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7775..7791)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7788..7810)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7799..7815
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7823..7829,7830..7847,7848..7853))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7868..7889
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8006..8023)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8177..8765)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8257..8276)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8936..9727,9728..9796))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9559..9578
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9797..9901)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gagctcggta cctgacccgg tcgtgcccct ctctagagat aatgagcatt gcatgtctaa
       61 gttataaaaa attaccacat attttttttg tcacacttgt ttgaagtgca gtttatctat
      121 ctttatacat atatttaaac tttactctac gaataatata atctatagta ctacaataat
      181 atcagtgttt tagagaatca tataaatgaa cagttagaca tggtctaaag gacaattgag
      241 tattttgaca acaggactct acagttttat ctttttagtg tgcatgtgtt ctcctttttt
      301 tttgcaaata gcttcaccta tataatactt catccatttt attagtacat ccatttaggg
      361 tttagggtta atggttttta tagactaatt tttttagtac atctatttta ttctatttta
      421 gcctctaaat taagaaaact aaaactctat tttagttttt ttatttaata atttagatat
      481 aaaatagaat aaaataaagt gactaaaaat taaacaaata ccctttaaga aattaaaaaa
      541 actaaggaaa catttttctt gtttcgagta gataatgcca gcctgttaaa cgccgtcgac
      601 gagtctaacg gacaccaacc agcgaaccag cagcgtcgcg tcgggccaag cgaagcagac
      661 ggcacggcat ctctgtcgct gcctctggac ccctctcgat cgagagttcc gctccaccgt
      721 tggacttgct ccgctgtcgg catccagaaa ttgcgtggcg gagcggcaga cgtgagccgg
      781 cacggcaggc ggcctcctcc tcctctcacg gcaccggcag ctacggggga ttcctttccc
      841 accgctcctt cgctttccct tcctcgcccg ccgtaataaa tagacacccc ctccacaccc
      901 tctttcccca acctcgtgtt gttcggagcg cacacacaca caaccagatc tcccccaaat
      961 ccacccgtcg gcacctccgc ttcaaggtac gccgctcgtc ctcccccccc ccctctctac
     1021 cttctctaga tcggcgttcc ggtccatggt tagggcccgg tagttctact tctgttcatg
     1081 tttgtgttag atccgtgttt gtgttagatc cgtgctgcta gcgttcgtac acggatgcga
     1141 cctgtacgtc agacacgttc tgattgctaa cttgccagtg tttctctttg gggaatcctg
     1201 ggatggctct agccgttccg cagacgggat cgatttcatg attttttttg tttcgttgca
     1261 tagggtttgg tttgcccttt tcctttattt caatatatgc cgtgcacttg tttgtcgggt
     1321 catcttttca tgcttttttt tgtcttggtt gtgatgatgt ggtctggttg ggcggtcgtt
     1381 ctagatcgga gtagaattaa ttctgtttca aactacctgg tggatttatt aattttggat
     1441 ctgtatgtgt gtgccataca tattcatagt tacgaattga agatgatgga tggaaatatc
     1501 gatctaggat aggtatacat gttgatgcgg gttttactga tgcatataca gagatgcttt
     1561 ttgttcgctt ggttgtgatg atgtggtgtg gttgggcggt cgttcattcg ttctagatcg
     1621 gagtagaata ctgtttcaaa ctacctggtg tatttattaa ttttggaact gtatgtgtgt
     1681 gtcatacatc ttcatagtta cgagtttaag atggatggaa atatcgatct aggataggta
     1741 tacatgttga tgtgggtttt actgatgcat atacatgatg gcatatgcag catctattca
     1801 tatgctctaa ccttgagtac ctatctatta taataaacaa gtatgtttta taattatttt
     1861 gatcttgata tacttggatg atggcatatg cagcagctat atgtggattt ttttagccct
     1921 gccttcatac gctatttatt tgcttggtac tgtttctttt gtcgatgctc accctgttgt
     1981 ttggtgttac ttctgcaaag cttgtcgacg gatccatgcc aaagaagaag aggaaggttc
     2041 ccgccagcaa gccttcccgc ttcaagccaa tgaggactga tccagcaaaa gcagatgaga
     2101 tcaggcctca tgtcggcaag gaccgcgcgg tggccaccct ctgggatgcc gacggcaaca
     2161 gggtgctagg gcttcactcc gctgatgatg acgggccggc ggccacggcg gcgtggaagc
     2221 cgccgtggag ggactacgtg cgcctccggc ggcatgttga agcgcacgcg gcggcaagaa
     2281 tgcatcaaga tgggcacaag acaatggtga tgtatatcaa cctcccgccg tgcaagtatt
     2341 ttgatggttg taaactaaac ctggaggaca tattgcccaa gggaagcacg ctgtggatgc
     2401 accgcgtctt ccagaatggt gggacaaaaa tttaccagtt caacggcacc ggcagagcct
     2461 atgttaagtc cggcagcgag acgccaggca cctccgagag cgctacgcct gaacttaagg
     2521 acaagaagta ctcgatcggc ctcgccatcg ggacgaactc agttggctgg gccgtgatca
     2581 ccgacgagta caaggtgccc tctaagaagt tcaaggtcct ggggaacacc gaccgccatt
     2641 ccatcaagaa gaacctcatc ggcgctctcc tgttcgacag cggggagacc gctgaggcta
     2701 cgaggctcaa gagaaccgct aggcgccggt acacgagaag gaagaacagg atctgctacc
     2761 tccaagagat tttctccaac gagatggcca aggttgacga ttcattcttc caccgcctgg
     2821 aggagtcttt cctcgtggag gaggataaga agcacgagcg gcatcccatc ttcggcaaca
     2881 tcgtggacga ggttgcctac cacgagaagt accctacgat ctaccatctg cggaagaagc
     2941 tcgtggactc caccgataag gcggacctca gactgatcta cctcgctctg gcccacatga
     3001 tcaagttccg cggccatttc ctgatcgagg gggatctcaa cccagacaac agcgatgttg
     3061 acaagctgtt catccaactc gtgcagacct acaaccaact cttcgaggag aacccgatca
     3121 acgcctctgg cgtggacgcg aaggctatcc tgtccgcgag gctctcgaag tccaggaggc
     3181 tggagaacct gatcgctcag ctcccaggcg agaagaagaa cggcctgttc gggaacctca
     3241 tcgctctcag cctggggctc accccgaact tcaagtcgaa cttcgatctc gctgaggacg
     3301 ccaagctgca actctccaag gacacctacg acgatgacct cgataacctc ctggcccaga
     3361 tcggcgatca atacgcggac ctgttcctcg ctgccaagaa cctgtcggac gccatcctcc
     3421 tgtcagatat cctccgcgtg aacaccgaga tcacgaaggc tccactctct gcctccatga
     3481 tcaagcgcta cgacgagcac catcaggatc tgaccctcct gaaggcgctg gtccgccaac
     3541 agctcccgga gaagtacaag gagattttct tcgatcagtc gaagaacggc tacgctgggt
     3601 acatcgacgg cggggcctca caagaggagt tctacaagtt catcaagcca atcctggaga
     3661 agatggacgg cacggaggag ctcctggtga agctcaacag ggaggacctc ctgcggaagc
     3721 agagaacctt cgataacggc agcatccccc accaaatcca tctcggggag ctgcacgcca
     3781 tcctgagaag gcaagaggac ttctaccctt tcctcaagga taaccgggag aagatcgaga
     3841 agatcctgac cttcagaatc ccatactacg tcggccctct cgcgcggggg aactcaagat
     3901 tcgcttggat gacccgcaag tctgaggaga ccatcacgcc gtggaacttc gaggaggtgg
     3961 tggacaaggg cgctagcgct cagtcgttca tcgagaggat gaccaacttc gacaagaacc
     4021 tgcccaacga gaaggtgctc cctaagcact cgctcctgta cgagtacttc accgtctaca
     4081 acgagctcac gaaggtgaag tacgtcaccg agggcatgcg caagccagcg ttcctgtccg
     4141 gggagcagaa gaaggctatc gtggacctcc tgttcaagac caaccggaag gtcacggtta
     4201 agcaactcaa ggaggactac ttcaagaaga tcgagtgctt cgattcggtc gagatcagcg
     4261 gcgttgagga ccgcttcaac gccagcctcg ggacctacca cgatctcctg aagatcatca
     4321 aggataagga cttcctggac aacgaggaga acgaggatat cctggaggac atcgtgctga
     4381 ccctcacgct gttcgaggac agggagatga tcgaggagcg cctgaagacg tacgcccatc
     4441 tcttcgatga caaggtcatg aagcaactca agcgccggag atacaccggc tgggggaggc
     4501 tgtcccgcaa gctcatcaac ggcatccggg acaagcagtc cgggaagacc atcctcgact
     4561 tcctcaagag cgatggcttc gccaacagga acttcatgca actgatccac gatgacagcc
     4621 tcaccttcaa ggaggatatc caaaaggctc aagtgagcgg ccagggggac tcgctgcacg
     4681 agcatatcgc gaacctcgct ggctcccccg cgatcaagaa gggcatcctc cagaccgtga
     4741 aggttgtgga cgagctcgtg aaggtcatgg gccggcacaa gcctgagaac atcgtcatcg
     4801 agatggccag agagaaccaa accacgcaga aggggcaaaa gaactctagg gagcgcatga
     4861 agcgcatcga ggagggcatc aaggagctgg ggtcccaaat cctcaaggag cacccagtgg
     4921 agaacaccca actgcagaac gagaagctct acctgtacta cctccagaac ggcagggata
     4981 tgtacgtgga ccaagagctg gatatcaacc gcctcagcga ttacgacgtc gatcatatcg
     5041 ttccccagtc tttcctgaag gatgactcca tcgacaacaa ggtcctcacc aggtcggaca
     5101 agaaccgcgg caagtcagat aacgttccat ctgaggaggt cgttaagaag atgaagaact
     5161 actggaggca gctcctgaac gccaagctga tcacgcaaag gaagttcgac aacctcacca
     5221 aggctgagag aggcgggctc tcagagctgg acaaggccgg cttcatcaag cggcagctgg
     5281 tcgagaccag acaaatcacg aagcacgttg cgcaaatcct cgactctcgg atgaacacga
     5341 agtacgatga gaacgacaag ctgatcaggg aggttaaggt gatcaccctg aagtctaagc
     5401 tcgtctccga cttcaggaag gatttccagt tctacaaggt tcgcgagatc aacaactacc
     5461 accatgccca tgacgcttac ctcaacgctg tggtcggcac cgctctgatc aagaagtacc
     5521 caaagctgga gtccgagttc gtgtacgggg actacaaggt ttacgatgtg cgcaagatga
     5581 tcgccaagtc ggagcaagag atcggcaagg ctaccgccaa gtacttcttc tactcaaaca
     5641 tcatgaactt cttcaagacc gagatcacgc tggccaacgg cgagatccgg aagagaccgc
     5701 tcatcgagac caacggcgag acgggggaga tcgtgtggga caagggcagg gatttcgcga
     5761 ccgtccgcaa ggttctctcc atgccccagg tgaacatcgt caagaagacc gaggtccaaa
     5821 cgggcgggtt ctcaaaggag tctatcctgc ctaagcggaa cagcgacaag ctcatcgcca
     5881 gaaagaagga ctgggaccca aagaagtacg gcgggttcga cagccctacc gtggcctact
     5941 cggtcctggt tgtggcgaag gttgagaagg gcaagtccaa gaagctcaag agcgtgaagg
     6001 agctcctggg gatcaccatc atggagaggt ccagcttcga gaagaaccca atcgacttcc
     6061 tggaggccaa gggctacaag gaggtgaaga aggacctgat catcaagctc ccgaagtact
     6121 ctctcttcga gctggagaac ggcaggaaga gaatgctggc ttccgctggc gagctccaga
     6181 aggggaacga gctcgcgctg ccaagcaagt acgtgaactt cctctacctg gcttcccact
     6241 acgagaagct caagggcagc ccggaggaca acgagcaaaa gcagctgttc gtcgagcagc
     6301 acaagcatta cctcgacgag atcatcgagc aaatctccga gttcagcaag cgcgtgatcc
     6361 tcgccgacgc gaacctggat aaggtcctct ccgcctacaa caagcaccgg gacaagccca
     6421 tcagagagca agcggagaac atcatccatc tcttcaccct gacgaacctc ggcgctcctg
     6481 ctgctttcaa gtacttcgac accacgatcg atcggaagag atacacctcc acgaaggagg
     6541 tcctggacgc gaccctcatc caccagtcga tcaccggcct gtacgagacg aggatcgacc
     6601 tctcacaact cggcggggat aagagacccg cagcaaccaa gaaggcaggg caagcaaaga
     6661 agaagaagac gcgtgactcc ggcggcagca ccaacctgtc cgacatcatc gagaaggaga
     6721 cgggcaagca actcgtgatc caggagagca tcctcatgct gccagaggag gtggaggagg
     6781 tcatcggcaa caagccagag tccgacatcc tggtgcacac cgcctacgac gagtccaccg
     6841 acgagaacgt catgctcctg accagcgacg ccccagagta caagccatgg gccctcgtca
     6901 tccaggacag caacggggag aacaagatca agatgctgtc gggggggagc ccaaagaaga
     6961 agcggaaggt gtagtgtaca agtaacccgg gaattcggta cgctgaaatc accagtctct
     7021 ctctacaaat ctatctctct ctattttctc cataaataat gtgtgagtag tttcccgata
     7081 agggaaatta gggttcttat agggtttcgc tcatgtgttg agcatataag aaacccttag
     7141 tatgtatttg tatttgtaaa atacttctat caataaaatt tctaattcct aaaaccaaaa
     7201 tccagtacta aaatccagat ctcctaaagt ccctatagat ctttgtcgtg aatataaacc
     7261 agacacgaga cgactaaacc tggagcccag acgccgttcg aagctagaag taccgcttag
     7321 gcaggaggcc gttagggaaa agatgctaag gcagggttgg ttacgttgac tcccccgtag
     7381 gtttggttta aatatgatga agtggacgga aggaaggagg aagacaagga aggataaggt
     7441 tgcaggccct gtgcaaggta agaagatgga aatttgatag aggtacgcta ctatacttat
     7501 actatacgct aagggaatgc ttgtatttat accctatacc ccctaataac cccttatcaa
     7561 tttaagaaat aatccgcata agcccccgct taaaaattgg tatcagagcc atgaataggt
     7621 ctatgaccaa aactcaagag gataaaacct caccaaaata cgaaagagtt cttaactcta
     7681 aagataaaag atctttcaag atcaaaacta gttccctcac accggtgacg gggatcgcat
     7741 gcgatatctc gagatctagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     7801 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     7861 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     7921 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     7981 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     8041 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     8101 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     8161 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     8221 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     8281 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     8341 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     8401 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     8461 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     8521 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     8581 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     8641 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     8701 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     8761 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     8821 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     8881 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     8941 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     9001 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     9061 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     9121 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     9181 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     9241 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     9301 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     9361 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     9421 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     9481 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     9541 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     9601 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     9661 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     9721 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     9781 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     9841 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     9901 gcacatttcc ccgaaaagtg ccacctgcca gtgccaagct aattc
//
