LOCUS       sequence-404961-        3948 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo lvl0 3'UTR+Terminator part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3948)
  AUTHORS   Yang EJY, Nemhauser JL
  TITLE     Building a pipeline to identify and engineer constitutive and 
            repressible promoters.
  JOURNAL   Quant Plant Biol. 2023 Oct 19;4:e12. doi: 10.1017/qpb.2023.10. 
            eCollection 2023.
  PUBMED    37901686
REFERENCE   2  (bases 1 to 3948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Quant Plant
            Biol."; date: "2023-10-19"; volume: "4:e12. doi"; pages: " 
            10.1017/qpb.2023.10. eCollection 2023"
COMMENT     Sequence Label: pICH41276(3Uter)-tAT5G42470
FEATURES             Location/Qualifiers
     source          1..3948
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      282..870
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     771..790
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1024..1041
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1518..1536)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2942..3046
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3268..3948,1..111)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa
       61 gaatttgtcc attacgtaaa aggcgagatc accaaggtag tcggcaaata actgtcagac
      121 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
      181 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
      241 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
      301 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
      361 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
      421 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
      481 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
      541 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
      601 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
      661 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
      721 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
      781 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
      841 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
      901 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
      961 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     1021 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     1081 gcgcgttggc cgattcatta atcactctgt ggtctcagct ttgtatctat ttcgaaagtt
     1141 agtattgtcc aaattttatg tgacactcct cttgctatgt aaactgaaat ggattactgt
     1201 tctgcattaa ggagaataca attataagca atttgtcttg atttcaacaa gattttgctt
     1261 ggctatagga ttcattggct ctgtttgctt ttacatttac atgtcataat agtttcgaat
     1321 tttacacatt tcagttggat gttaagaaaa gagagggaat tgatggggtt ttgtgggttt
     1381 aaactttaaa gtagtcaaga attaagtcat tggtttactg ttgctctata tgtgtaaaat
     1441 gaaggcaact ccaacggttc ttaggtggaa tagattatcg cttgagacca cgaagtggct
     1501 cttcagtgga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     1561 aatggtttct tagacgtcag gtgctgatga atcccctaat gatttttatc aaaatcatta
     1621 agttaaggta gatacacatc ttgtcatatg atcaaatggt ttcgccaaaa atcaataatc
     1681 agacaacaaa atgtgcgaac tcgatatttt acacgactct ctttaccaat tctgccccga
     1741 attacactta aaacgactca acagcttaac gttggcttgc cacgccttac ttgactgtaa
     1801 aactctcact cttaccgaac ttggccgtaa cctgccaacc aaagcgagaa caaaacataa
     1861 catcaaacga atcgaccgat tgttaggtaa tcgtcacctc cacaaagagc gactcgctgt
     1921 ataccgttgg catgctagct ttatctgttc gggcaatacg atgcccattg tacttgttga
     1981 ctggtctgat atccgtgagc aaaaacggct tatggtattg cgagcttcag tcgcactaca
     2041 cggtcgttct gttactcttt atgagaaagc gttcccgctt tcagagcaat gttcaaagaa
     2101 agctcatgac caatttctag ccgaccttgc gagcattcta ccgagtaaca ccacaccgct
     2161 cattgtcagt gatgctggct ttaaagtgcc atggtataaa tccgttgaga agctgggttg
     2221 gtactggtta agtcgagtaa gaggaaaagt acaatatgca gacctaggag cggaaaactg
     2281 gaaacctatc agcaacttac atgatatgtc atctagtcac tcaaagactt taggctataa
     2341 gaggctgact aaaagcaacc caatctcatg ccaaattcta ttgtataaat ctcgctctaa
     2401 aggccgaaaa aatcagcgct cgacacggac tcattgtcac cacccgtcac ctaaaatcta
     2461 ctcagcgtcg gcaaaggagc catggattct agcaactaac ttacctgttg aaattcgaac
     2521 acccaaacaa cttgttaata tctattcgaa gcgaatgcag attgaagaaa ccttccgaga
     2581 cttgaaaagt cctgcctacg gactaggcct acgccatagc cgaacgagca gctcagagcg
     2641 ttttgatatc atgctgctaa tcgccctgat gcttcaacta acatgttggc ttgcgggcgt
     2701 tcatgctcag aaacaaggtt gggacaagca cttccaggct aacacagtca gaaatcgaaa
     2761 cgtactctca acagttcgct taggcatgga agttttgcgg cattctggct acacaataac
     2821 aagggaagac tcactcgtgg ctgcaaccct gcttactcaa aatctattca cacatggtta
     2881 cgttttgggg aaattatgag gggatctctc agcgtcaggt ggcacttttc ggggaaatgt
     2941 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     3001 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatgc gctcacgcaa
     3061 ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc
     3121 ttgttatgac tgtttttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg
     3181 cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc
     3241 agtcgcccta aaacaaagtt aaacatcatg agggaagcgg tgatcgccga agtatcgact
     3301 caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta
     3361 catttgtacg gctccgcagt ggatggcggc ctgaagccac acagcgatat tgatttgctg
     3421 gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg
     3481 gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt
     3541 gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa
     3601 tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg
     3661 gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag
     3721 gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg
     3781 ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc
     3841 cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg
     3901 gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagct
//
