LOCUS       sequence-405090-       15234 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid allows for inducible expression of engineered 
            BRD4-p300(H*IT) fusion tagged with mNeonGreen, in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15234)
  AUTHORS   Kosno M, Currie SL, Kumar A, Xing C, Rosen MK
  TITLE     Molecular features driving condensate formation and gene expression 
            by the BRD4-NUT fusion oncoprotein are overlapping but distinct.
  JOURNAL   Sci Rep. 2023 Jul 24;13(1):11907. doi: 10.1038/s41598-023-39102-9.
  PUBMED    37488172
REFERENCE   2  (bases 1 to 15234)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2023-07-24"; volume: "13(1):11907. doi"; pages: " 
            10.1038/s41598-023-39102-9"
COMMENT     Sequence Label: pcDNA5-FRT/TO-mNeonGreen-BRD4-p300(H*IT)
FEATURES             Location/Qualifiers
     source          1..15234
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             990..1016
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             1020..1724
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="VSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYEE
                     LNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFEDG
                     ASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIST
                     FKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQK
                     AFTDVMGMDELYK"
     CDS             7041..8891
                     /codon_start=1
                     /gene="EP300"
                     /product="catalytic histone acetyltransferase core of the 
                     human E1A-associated protein p300, inactivated by the 
                     D1399Y point mutation (Hilton et al., 2015)"
                     /label=p300 core (D1399Y)
                     /translation="IFKPEELRQALMPTLEALYRQDPESLPFRQPVDPQLLGIPDYFDI
                     VKSPMDLSTIKRKLDTGQYQEPWQYVDDIWLMFNNAWLYNRKTSRVYKYCSKLSEVFEQ
                     EIDPVMQSLGYCCGRKLEFSPQTLCCYGKQLCTIPRDATYYSYQNRYHFCEKCFNEIQG
                     ESVSLGDDPSQPQTTINKEQFSKRKNDTLDPELFVECTECGRKMHQICVLHHEIIWPAG
                     FVCDGCLKKSARTRKENKFSAKRLPSTRLGTFLENRVNDFLRRQNHPESGEVTVRVVHA
                     SDKTVEVKPGMKARFVDSGEMAESFPYRTKALFAFEEIDGVDLCFFGMHVQEYGSDCPP
                     PNQRRVYISYLYSVHFFRPKCLRTAVYHEILIGYLEYVKKLGYTTGHIWACPPSEGDDY
                     IFHCHPPDQKIPKPKRLQEWYKKMLDKAVSERIVHDYKDIFKQATEDRLTSAKELPYFE
                     GDFWPNVLEESIKELEQEEEERKREENTSNESTDVTKGDSKNAKKKNNKKTSKNKSSLS
                     RGNKKKPGMPNVSNDLSQKLYATMEKHKEVFFVIRLIAGPAANSLPPIVDPDPLIPCDL
                     MDGRDAFLTLARDKHLEFSSLRRAQWSTMCMLVELHTQSQD"
     regulatory      8655..8664
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(11186..11203)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    11192..11416
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(11549..11568)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    11700..11747
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             11755..12777
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             11755..12777
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    12907..13028
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12944..12963)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12998..13017
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(13077..13093)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13077..13093)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(13090..13112)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    13101..13117
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(13125..13131,13132..13149,13150..13155))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13170..13191
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(13308..13325)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13479..14067)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13559..13578)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(14238..15029,15030..15098))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14861..14880
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(15099..15203)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttatgggac cggctgccaa gcgcgtgaaa ctcgatggcg
     1021 tgagcaaggg cgaggaggat aacatggcct ctctcccagc gacacatgag ttacacatct
     1081 ttggctccat caacggtgtg gactttgaca tggtgggtca gggcaccggc aatccaaatg
     1141 atggttatga ggagttaaac ctgaagtcca ccaagggtga cctccagttc tccccctgga
     1201 ttctggtccc tcatatcggg tatggcttcc atcagtacct gccctaccct gacgggatgt
     1261 cgcctttcca ggccgcaatg gtagatggct ccggatacca agtccatcgc acaatgcagt
     1321 ttgaagatgg tgcctccctt actgttaact accgctacac ctacgaggga agccacatca
     1381 aaggagaggc ccaggtgaag gggactggtt tccctgctga cggtcctgtg atgaccaact
     1441 cgctgaccgc tgcggactgg tgcaggtcga agaagactta ccccaacgac aaaaccatca
     1501 tcagtacctt taagtggagt tacaccactg gaaatggcaa gcgctaccgg agcactgcgc
     1561 ggaccaccta cacctttgcc aagccaatgg cggctaacta tctgaagaac cagccgatgt
     1621 acgtgttccg taagacggaa ctcaagcact ccaagaccga actcaacttc aaggagtggc
     1681 aaaaggcctt taccgatgtg atgggcatgg acgagctgta caagggcggc tcgtacgtgt
     1741 acaagtctgc ggagagcggc cctgggacga gattgagaaa tctgccagta atgggggatg
     1801 gactagaaac ttcccaaatg tctacaacac aggcccaggc ccaaccccag ccagccaacg
     1861 cagccagcac caaccccccg cccccagaga cctccaaccc taacaagccc aagaggcaga
     1921 ccaaccaact gcaatacctg ctcagagtgg tgctcaagac actatggaaa caccagtttg
     1981 catggccttt ccagcagcct gtggatgccg tcaagctgaa cctccctgat tactataaga
     2041 tcattaaaac gcctatggat atgggaacaa taaagaagcg cttggaaaac aactattact
     2101 ggaatgctca ggaatgtatc caggacttca acactatgtt tacaaattgt tacatctaca
     2161 acaagcctgg agatgacata gtcttaatgg cagaagctct ggaaaagctc ttcttgcaaa
     2221 aaataaatga gctacccaca gaagaaaccg agatcatgat agtccaggca aaaggaagag
     2281 gacgtgggag gaaagaaaca gggacagcaa aacctggcgt ttccacggta ccaaacacaa
     2341 ctcaagcatc gactcctccg cagacccaga cccctcagcc gaatcctcct cctgtgcagg
     2401 ccacgcctca ccccttccct gccgtcaccc cggacctcat cgtccagacc cctgtcatga
     2461 cagtggtgcc tccccagcca ctgcagacgc ccccgccagt gcccccccag ccacaacccc
     2521 cacccgctcc agctccccag cccgtacaga gccacccacc catcatcgcg gccaccccac
     2581 agcctgtgaa gacaaagaag ggagtgaaga ggaaagcaga caccaccacc cccaccacca
     2641 ttgaccccat tcacgagcca ccctcgctgc ccccggagcc caagaccacc aagctgggcc
     2701 agcggcggga gagcagccgg cctgtgaaac ctccaaagaa ggacgtgccc gactctcagc
     2761 agcacccagc accagagaag agcagcaagg tctcggagca gctcaagtgc tgcagcggca
     2821 tcctcaagga gatgtttgcc aagaagcacg ccgcctacgc ctggcccttc tacaagcctg
     2881 tggacgtgga ggcactgggc ctacacgact actgtgacat catcaagcac cccatggaca
     2941 tgagcacaat caagtctaaa ctggaggccc gtgagtaccg tgatgctcag gagtttggtg
     3001 ctgacgtccg attgatgttc tccaactgct ataagtacaa ccctcctgac catgaggtgg
     3061 tggccatggc ccgcaagctc caggatgtgt tcgaaatgcg ctttgccaag atgccggacg
     3121 agcctgagga gccagtggtg gccgtgtcct ccccggcagt gccccctccc accaaggttg
     3181 tggccccgcc ctcatccagc gacagcagca gcgatagctc ctcggacagt gacagttcga
     3241 ctgatgactc tgaggaggag cgagcccagc ggctggctga gctccaggag cagctcaaag
     3301 ccgtgcacga gcagcttgca gccctctctc agccccagca gaacaaacca aagaaaaagg
     3361 agaaagacaa gaaggaaaag aaaaaagaaa agcacaaaag gaaagaggaa gtggaagaga
     3421 ataaaaaaag caaagccaag gaacctcctc ctaaaaagac gaagaaaaat aatagcagca
     3481 acagcaatgt gagcaagaag gagccagcgc ccatgaagag caagccccct cccacgtatg
     3541 agtcggagga agaggacaag tgcaagccta tgtcctatga ggagaagcgg cagctcagct
     3601 tggacatcaa caagctcccc ggcgagaagc tgggccgcgt ggtgcacatc atccagtcac
     3661 gggagccctc cctgaagaat tccaaccccg acgagattga aatcgacttt gagaccctga
     3721 agccgtccac actgcgtgag ctggagcgct atgtcacctc ctgtttgcgg aagaaaagga
     3781 aacctcaagc tgagaaagtt gatgtgattg ccggctcctc caagatgaag ggcttctcgt
     3841 cctcagagtc ggagagctcc agtgagtcca gctcctctga cagcgaagac tccgaaacaa
     3901 tggccgagaa tgtggtggaa ccggggccgc cttcagccaa gcggcctaaa ctctcatctc
     3961 cggccctctc ggcgtccgcc agcgatggca cagattttgg ctctctattt gacttggagc
     4021 acgacttacc agatgaatta atcaactcta cagaattggg actaaccaat ggtggtgata
     4081 ttaatcagct tcagacaagt cttggcatgg tacaagatgc agcttctaaa cataaacagc
     4141 tgtcagaatt gctgcgatct ggtagttccc ctaacctcaa tatgggagtt ggtggcccag
     4201 gtcaagtcat ggccagccag gcccaacaga gcagtcctgg attaggtttg ataaatagca
     4261 tggtcaaaag cccaatgaca caggcaggct tgacttctcc caacatgggg atgggcacta
     4321 gtggaccaaa tcagggtcct acgcagtcaa caggtatgat gaacagtcca gtaaatcagc
     4381 ctgccatggg aatgaacaca gggatgaatg cgggcatgaa tcctggaatg ttggctgcag
     4441 gcaatggaca agggataatg cctaatcaag tcatgaacgg ttcaattgga gcaggccgag
     4501 ggcgacagaa tatgcagtac ccaaacccag gcatgggaag tgctggcaac ttactgactg
     4561 agcctcttca gcagggctct ccccagatgg gaggacaaac aggattgaga ggcccccagc
     4621 ctcttaagat gggaatgatg aacaacccca atccttatgg ttcaccatat actcagaatc
     4681 ctggacagca gattggagcc agtggccttg gtctccagat tcagacaaaa actgtactat
     4741 caaataactt atctccattt gctatggaca aaaaggcagt tcctggtgga ggaatgccca
     4801 acatgggtca acagccagcc ccgcaggtcc agcagccagg tctggtgact ccagttgccc
     4861 aagggatggg ttctggagca catacagctg atccagagaa gcgcaagctc atccagcagc
     4921 agcttgttct ccttttgcat gctcacaagt gccagcgccg ggaacaggcc aatggggaag
     4981 tgaggcagtg caaccttccc cactgtcgca caatgaagaa tgtcctaaac cacatgacac
     5041 actgccagtc aggcaagtct tgccaagtgg cacactgtgc atcttctcga caaatcattt
     5101 cacactggaa gaattgtaca agacatgatt gtcctgtgtg tctccccctc aaaaatgctg
     5161 gtgataagag aaatcaacag ccaattttga ctggagcacc cgttggactt ggaaatccta
     5221 gctctctagg ggtgggtcaa cagtctgccc ccaacctaag cactgttagt cagattgatc
     5281 ccagctccat agaaagagcc tatgcagctc ttggactacc ctatcaagta aatcagatgc
     5341 cgacacaacc ccaggtgcaa gcaaagaacc agcagaatca gcagcctggg cagtctcccc
     5401 aaggcatgcg gcccatgagc aacatgagtg ctagtcctat gggagtaaat ggaggtgtag
     5461 gagttcaaac gccgagtctt ctttctgact caatgttgca ttcagccata aattctcaaa
     5521 acccaatgat gagtgaaaat gccagtgtgc cctccatggg tcctatgcca acagcagctc
     5581 aaccatccac tactggaatt cggaaacagt ggcacgaaga tattactcag gatcttcgaa
     5641 atcatcttgt tcacaaactc gtccaagcca tatttcctac gccggatcct gctgctttaa
     5701 aagacagacg gatggaaaac ctagttgcat atgctcggaa agttgaaggg gacatgtatg
     5761 aatctgcaaa caatcgagcg gaatactacc accttctagc tgagaaaatc tataagatcc
     5821 agaaagaact agaagaaaaa cgaaggacca gactacagaa gcagaacatg ctaccaaatg
     5881 ctgcaggcat ggttccagtt tccatgaatc cagggcctaa catgggacag ccgcaaccag
     5941 gaatgacttc taatggccct ctacctgacc caagtatgat ccgtggcagt gtgccaaacc
     6001 agatgatgcc tcgaataact ccacaatctg gtttgaatca atttggccag atgagcatgg
     6061 cccagccccc tattgtaccc cggcaaaccc ctcctcttca gcaccatgga cagttggctc
     6121 aacctggagc tctcaacccg cctatgggct atgggcctcg tatgcaacag ccttccaacc
     6181 agggccagtt ccttcctcag actcagttcc catcacaggg aatgaatgta acaaatatcc
     6241 ctttggctcc gtccagcggt caagctccag tgtctcaagc acaaatgtct agttcttcct
     6301 gcccggtgaa ctctcctata atgcctccag ggtctcaggg gagccacatt cactgtcccc
     6361 agcttcctca accagctctt catcagaatt caccctcgcc tgtacctagt cgtaccccca
     6421 cccctcacca tactccccca agcatagggg ctcagcagcc accagcaaca acaattccag
     6481 cccctgttcc tacacctcct gccatgccac ctgggccaca gtcccaggct ctacatcccc
     6541 ctccaaggca gacacctaca ccaccaacaa cacaacttcc ccaacaagtg cagccttcac
     6601 ttcctgctgc accttctgct gaccagcccc agcagcagcc tcgctcacag cagagcacag
     6661 cagcgtctgt tcctacccca acagcaccgc tgcttcctcc gcagcctgca actccacttt
     6721 cccagccagc tgtaagcatt gaaggacagg tatcaaatcc tccatctact agtagcacag
     6781 aagtgaattc tcaggccatt gctgagaagc agccttccca ggaagtgaag atggaggcca
     6841 aaatggaagt ggatcaacca gaaccagcag atactcagcc ggaggatatt tcagagtcta
     6901 aagtggaaga ctgtaaaatg gaatctaccg aaacagaaga gagaagcact gagttaaaaa
     6961 ctgaaataaa agaggaggaa gaccagccaa gtacttcagc tacccagtca tctccggctc
     7021 caggacagtc aaagaaaaag attttcaaac cagaagaact acgacaggca ctgatgccaa
     7081 ctttggaggc actttaccgt caggatccag aatcccttcc ctttcgtcaa cctgtggacc
     7141 ctcagctttt aggaatccct gattactttg atattgtgaa gagccccatg gatctttcta
     7201 ccattaagag gaagttagac actggacagt atcaggagcc ctggcagtat gtcgatgata
     7261 tttggcttat gttcaataat gcctggttat ataaccggaa aacatcacgg gtatacaaat
     7321 actgctccaa gctctctgag gtctttgaac aagaaattga cccagtgatg caaagccttg
     7381 gatactgttg tggcagaaag ttggagttct ctccacagac actgtgttgc tacggcaaac
     7441 agttgtgcac aatacctcgt gatgccactt actacagtta ccagaacagg tatcatttct
     7501 gtgagaagtg tttcaatgag atccaagggg agagcgtttc tttgggggat gacccttccc
     7561 agcctcaaac tacaataaat aaagaacaat tttccaagag aaaaaatgac acactggatc
     7621 ctgaactgtt tgttgaatgt acagagtgcg gaagaaagat gcatcagatc tgtgtccttc
     7681 accatgagat catctggcct gctggattcg tctgtgatgg ctgtttaaag aaaagtgcac
     7741 gaactaggaa agaaaataag ttttctgcta aaaggttgcc atctaccaga cttggcacct
     7801 ttctagagaa tcgtgtgaat gactttctga ggcgacagaa tcaccctgag tcaggagagg
     7861 tcactgttag agtagttcat gcttctgaca aaaccgtgga agtaaaacca ggcatgaaag
     7921 caaggtttgt ggacagtgga gagatggcag aatcctttcc ataccgaacc aaagccctct
     7981 ttgcctttga agaaattgat ggtgttgacc tgtgcttctt tggcatgcat gttcaagagt
     8041 atggctctga ctgccctcca cccaaccaga ggagagtata catatcttac ctctatagtg
     8101 ttcatttctt ccgtcctaaa tgcttgagga ctgcagtcta tcatgaaatc ctaattggat
     8161 atttagaata tgtcaagaaa ttaggttaca caacagggca tatttgggca tgtccaccaa
     8221 gtgagggaga tgattatatc ttccattgcc atcctcctga ccagaagata cccaagccca
     8281 agcgactgca ggaatggtac aaaaaaatgc ttgacaaggc tgtatcagag cgtattgtcc
     8341 atgactacaa ggatattttt aaacaagcta ctgaagatag attaacaagt gcaaaggaat
     8401 tgccttattt cgagggtgat ttctggccca atgttctgga agaaagcatt aaggaactgg
     8461 aacaggagga agaagagaga aaacgagagg aaaacaccag caatgaaagc acagatgtga
     8521 ccaagggaga cagcaaaaat gctaaaaaga agaataataa gaaaaccagc aaaaataaga
     8581 gcagcctgag taggggcaac aagaagaaac ccgggatgcc caatgtatct aacgacctct
     8641 cacagaaact atatgccacc atggagaagc ataaagaggt cttctttgtg atccgcctca
     8701 ttgctggccc tgctgccaac tccctgcctc ccattgttga tcctgatcct ctcatcccct
     8761 gcgatctgat ggatggtcgg gatgcgtttc tcacgctggc aagggacaag cacctggagt
     8821 tctcttcact ccgaagagcc cagtggtcca ccatgtgcat gctggtggag ctgcacacgc
     8881 agagccagga ccgctttgtc tacacctgca atgaatgcaa gcaccatgtg gagacacgct
     8941 ggcactgtac tgtctgtgag gattatgact tgtgtatcac ctgctataac actaaaaacc
     9001 atgaccacaa aatggagaaa ctaggccttg gcttagatga tgagagcaac aaccagcagg
     9061 ctgcagccac ccagagccca ggcgattctc gccgcctgag tatccagcgc tgcatccagt
     9121 ctctggtcca tgcttgccag tgtcggaatg ccaattgctc actgccatcc tgccagaaga
     9181 tgaagcgggt tgtgcagcat accaagggtt gcaaacggaa aaccaatggc gggtgcccca
     9241 tctgcaagca gctcattgcc ctctgctgct accatgccaa gcactgccag gagaacaaat
     9301 gcccggtgcc gttctgccta aacatcaagc agaagctccg gcagcaacag ctgcagcacc
     9361 gactacagca ggcccaaatg cttcgcagga ggatggccag catgcagcgg actggtgtgg
     9421 ttgggcagca acagggcctc ccttccccca ctcctgccac tccaacgaca ccaactggcc
     9481 aacagccaac caccccgcag acgccccagc ccacttctca gcctcagcct acccctccca
     9541 atagcatgcc accctacttg cccaggactc aagctgctgg ccctgtgtcc cagggtaagg
     9601 cagcaggcca ggtgacccct ccaacccctc ctcagactgc tcagccaccc cttccaggac
     9661 ccccacctgc agcagtggaa atggcaatgc agattcagag agcagcggag acgcagcgcc
     9721 agatggccca cgtgcaaatt tttcaaaggc caatccaaca ccagatgccc ccgatgactc
     9781 ccatggcccc catgggtatg aacccacctc ccatgaccag aggtcccagt gggcatttgg
     9841 agccagggat gggaccgaca gggatgcagc aacagccacc ctggagccaa ggaggattgc
     9901 ctcagcccca gcaactacag tctgggatgc caaggccagc catgatgtca gtggcccagc
     9961 atggtcaacc tttgaacatg gctccacaac caggattggg ccaggtaggt atcagcccac
    10021 tcaaaccagg cactgtgtct caacaagcct tacaaaacct tttgcggact ctcaggtctc
    10081 ccagctctcc cctgcagcag caacaggtgc ttagtatcct tcacgccaac ccccagctgt
    10141 tggctgcatt catcaagcag cgggctgcca agtatgccaa ctctaatcca caacccatcc
    10201 ctgggcagcc tggcatgccc caggggcagc cagggctaca gccacctacc atgccaggtc
    10261 agcagggggt ccactccaat ccagccatgc agaacatgaa tccaatgcag gcgggcgttc
    10321 agagggctgg cctgccccag cagcaaccac agcagcaact ccagccaccc atgggaggga
    10381 tgagccccca ggctcagcag atgaacatga accacaacac catgccttca caattccgag
    10441 acatcttgag acgacagcaa atgatgcaac agcagcagca acagggagca gggccaggaa
    10501 taggccctgg aatggccaac cataaccagt tccagcaacc ccaaggagtt ggctacccac
    10561 cacagcagca gcagcggatg cagcatcaca tgcaacagat gcaacaagga aatatgggac
    10621 agataggcca gcttccccag gccttgggag cagaggcagg tgccagtcta caggcctatc
    10681 agcagcgact ccttcagcaa cagatggggt cccctgttca gcccaacccc atgagccccc
    10741 agcagcatat gctcccaaat caggcccagt ccccacacct acaaggccag cagatcccta
    10801 attctctctc caatcaagtg cgctctcccc agcctgtccc ttctccacgg ccacagtccc
    10861 agccccccca ctccagtcct tccccaagga tgcagcctca gccttctcca caccacgttt
    10921 ccccacagac aagttcccca catcctggac tggtagctgc ccaggccaac cccatggaac
    10981 aagggcattt tgccagcccg gaccagaatt caatgctttc tcagcttgct agcaatccag
    11041 gcatggcaaa cctccatggt gcaagcgcca cggacctggg actcagcacc gataactcag
    11101 acttgaattc aaacctctca cagagtacac tagacatatg agcggccgct cgagtctaga
    11161 gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
    11221 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
    11281 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
    11341 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
    11401 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc
    11461 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
    11521 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
    11581 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
    11641 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtacctag
    11701 aagttcctat tccgaagttc ctattctcta gaaagtatag gaacttcctt ggccaaaaag
    11761 cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc
    11821 gacctgatgc agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg
    11881 cgtggatatg tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt
    11941 tatcggcact ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggaattc
    12001 agcgagagcc tgacctattg catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg
    12061 cctgaaaccg aactgcccgc tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct
    12121 gcggccgatc ttagccagac gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa
    12181 tacactacat ggcgtgattt catatgcgcg attgctgatc cccatgtgta tcactggcaa
    12241 actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt
    12301 tgggccgagg actgccccga agtccggcac ctcgtgcacg cggatttcgg ctccaacaat
    12361 gtcctgacgg acaatggccg cataacagcg gtcattgact ggagcgaggc gatgttcggg
    12421 gattcccaat acgaggtcgc caacatcttc ttctggaggc cgtggttggc ttgtatggag
    12481 cagcagacgc gctacttcga gcggaggcat ccggagcttg caggatcgcc gcggctccgg
    12541 gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga gcttggttga cggcaatttc
    12601 gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg agccgggact
    12661 gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa
    12721 gtactcgccg atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa ggaatagcac
    12781 gtactacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
    12841 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
    12901 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
    12961 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
    13021 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca
    13081 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
    13141 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
    13201 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
    13261 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
    13321 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
    13381 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
    13441 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
    13501 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
    13561 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
    13621 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
    13681 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
    13741 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
    13801 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
    13861 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
    13921 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
    13981 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
    14041 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
    14101 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
    14161 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
    14221 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
    14281 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
    14341 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
    14401 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
    14461 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
    14521 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
    14581 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
    14641 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
    14701 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
    14761 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
    14821 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
    14881 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
    14941 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
    15001 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
    15061 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
    15121 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
    15181 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtc
//
