LOCUS       sequence-405409-       11465 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Helper plasmid for ORBIT in E. coli. Expresses RecT for oligo 
            recombineering and Bxb-1 for pInt incorporation. Has sacB for 
            counter selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11465)
  AUTHORS   Saunders SH, Ahmed AM
  TITLE     ORBIT for E. coli: Kilobase-scale oligonucleotide recombineering at 
            high throughput and high efficiency
  JOURNAL   bioRxiv 2023
REFERENCE   2  (bases 1 to 11465)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023"
COMMENT     Sequence Label: pHelper_Ec1_V1_gentR
FEATURES             Location/Qualifiers
     source          1..11465
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      850..877
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(938..1471)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(1660..1665,1666..1682,1683..1688))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        complement(1706..1810)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             complement(1907..2785)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     CDS             complement(3006..3971)
                     /codon_start=1
                     /gene="xylS"
                     /product="XylS regulator encoded by the Pseudomonas putida 
                     TOL plasmid pWWO"
                     /label=XylS
                     /note="stimulates transcription from the Pm promoter in the
                     presence of aromatic compounds such as m-toluate or 
                     benzoate"
                     /translation="MDFCLLNEKSQIFVHAEPYAVSDYVNQYVGTHSIRLPKGGRPAGR
                     LHHRIFGCLDLCRISYGGSVRVISPGLETCYHLQIILKGHCLWRGHGQEHYFAPGELLL
                     LNPDDQADLTYSEDCEKFIVKLPSVVLDRACSDNNWHKPREGIRFAARHNLQQLDGFIN
                     LLGLVCDEAEHTKSMPRVQEHYAGIIASKLLEMLGSNVSREIFSKGNPSFERVVQFIEE
                     NLKRNISLERLAELAMMSPRSLYNLFEKHAGTTPKNYIRNRKLESIRACLNDPSANVRS
                     ITEIALDYGFLHLGRFAENYRSAFGELPSDTLRQCKKEVA"
     promoter        4871..4951
                     /label=Pm promoter
                     /note="The bacterial Pm promoter is activated by XylS in 
                     the presence of benzoate or m-toluate (Marques et al., 
                     1999)."
     terminator      7775..7869
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     7872..7891
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     RBS             7976..7984
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             7987..9489
                     /codon_start=1
                     /transl_table=11
                     /gene="Bxb1"
                     /product="Bxb1 integrase"
                     /label=Bxb1 integrase
                     /note="Bxb1 large serine integrase from Mycobacterium 
                     bacteriophage Bxb1 (Ghosh et al. 2003)."
                     /protein_id="AFF61396.1"
                     /translation="MRALVVIRLSRVTDATTSPERQLESCQQLCAQRGWDVVGVAEDLD
                     VSGAVDPFDRKRRPNLARWLAFEEQPFDVIVAYRVDRLTRSIRHLQQLVHWAEDHKKLV
                     VSATEAHFDTTTPFAAVVIALMGTVAQMELEAIKERNRSAAHFNIRAGKYRGSLPPWGY
                     LPTRVDGEWRLVPDPVQRERILEVYHRVVDNHEPLHLVAHDLNRRGVLSPKDYFAQLQG
                     REPQGREWSATALKRSMISEAMLGYATLNGKTVRDDDGAPLVRAEPILTREQLEALRAE
                     LVKTSRAKPAVSTPSLLLRVLFCAVCGEPAYKFAGGGRKHPRYRCRSMGFPKHCGNGTV
                     AMAEWDAFCEEQVLDLLGDAERLEKVWVAGSDSAVELAEVNAELVDLTSLIGSPAYRAG
                     SPQREALDVRIAALAARQEELEGLEARPSGWEWRETGQRFGDWWREQDTAAKNTWLRSM
                     NVRLTFDVRGGLTRTIDFGDL*EYEQHLRLGSVVERLHTGMS"
     terminator      9524..9570
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             join(10009..10095,10096..11430)
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial 
                     growth sensitive to sucrose"
                     /label=SacB
                     /note="negative selection marker"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tttttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccat gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctaga actagttgat
      841 tagctaagca gaaggccatc ctgacggatg gcctttttgc gtttaataga agttttaaat
      901 caatctaaag tatatatgag taaacttggt ctgacagtta ggtggcggta cttgggtcga
      961 tatcaaagtg catcacttct tcccgtatgc ccaactttgt atagagagcc actgcgggat
     1021 cgtcaccgta atctgcttgc acgtagatca cataagcacc aagcgcgttg gcctcatgct
     1081 tgaggagatt gatgagcgcg gtggcaatgc cctgcctccg gtgctcgccg gagactgcga
     1141 gatcatagat atagatctca ctacgcggct gctcaaactt gggcagaacg taagccgcga
     1201 gagcgccaac aaccgcttct tggtcgaagg cagcaagcgc gatgaatgtc ttactacgga
     1261 gcaagttccc gaggtaatcg gagtccggct gatgttggga gtaggtggct acgtctccga
     1321 actcacgacc gaaaagatca agagcagccc gcatggattt gacttggtca gggccgagcc
     1381 tacatgtgcg aatgatgccc atacttgagc cacctaactt tgttttaggg cgactgccct
     1441 gctgcgtaac atcgttgctg ctgcgtaaca tcgttgctgc tccataacat caaacatcga
     1501 cccacggcgt aacgcgcttg ctgcttggat gcccgaggca tagactgtac aaaaaaacag
     1561 tcataacaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc ggtcaaggtt
     1621 ctggaccagt tgcgtgagcg catacgctac ttgcattaca gtttacgaac cgaacaggct
     1681 tatgtcaatt cggttgaata ctcatactct tcctttttca atattattga agcatttatc
     1741 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     1801 gggttccgcg cacatttccc cgaaaagtgg gtaataactg atataattaa attgaagctc
     1861 taatttgtga gtttagtata catgcattta cttataatac agttttttat gacaacttga
     1921 cggctacatc attcactttt tcttcacaac cggcacggaa ctcgctcggg ctggccccgg
     1981 tgcatttttt aaatacccgc gagaagtaga gttgatcgtc aaaaccaaca ttgcgaccga
     2041 cggtggcgat aggcatccgg gtggtgctca aaagcagctt cgcctggctg atacgttggt
     2101 cctcgcgcca gcttaagacg ctaatcccta actgctggcg gaaaagatgt gacagacgcg
     2161 acggcgacaa gcaaacatgc tgtgcgacgc tggcgatatc aaaattgctg tctgccaggt
     2221 gatcgctgat gtactgacaa gcctcgcgta cccgattatc catcggtgga tggagcgact
     2281 cgttaatcgc ttccatgcgc cgcagtaaca attgctcaag cagatttatc gccagcagct
     2341 ccgaatagcg cccttcccct tgcccggcgt taatgatttg cccaaacagg tcgctgaaat
     2401 gcggctggtg cgcttcatcc gggcgaaaga accccgtatt ggcaaatatt gacggccagt
     2461 taagccattc atgccagtag gcgcgcggac gaaagtaaac ccactggtga taccattcgc
     2521 gagcctccgg atgacgaccg tagtgatgaa tctctcctgg cgggaacagc aaaatatcac
     2581 ccggtcggca aacaaattct cgtccctgat ttttcaccac cccctgaccg cgaatggtga
     2641 gattgagaat ataacctttc attcccagcg gtcggtcgat aaaaaaatcg agataaccgt
     2701 tggcctcaat cggcgttaaa cccgccacca gatgggcatt aaacgagtat cccggcagca
     2761 ggggatcatt ttgcgcttca gccatacttt tcatactccc gccattcaga gaagaaacca
     2821 attgtccata ttgcatcaga cattgccgtc actgcgtctt ttactggctc ttctcgctaa
     2881 ccaaaccggt aaccccgctt attaaaagca ttctgtaaca aagcgggacc aaagccatga
     2941 caaaaacgcg taacaaaagt gtctataatc acggcagaaa agtccacatt gattatttgc
     3001 acggctcaag ccacttcctt tttgcattga cgcagggtgt cggaaggcaa ctcgccgaac
     3061 gcgctcctat agttttcagc gaagcgtccc aaatgtaaga agccgtagtc tagggctatc
     3121 tcagttatac tacgcacatt ggcactggga tcgttcaagc aggcgcggat gctttcgagc
     3181 ttgcggttgc ggatgtagtt cttcggcgtg gtgccggcgt gcttctcgaa caaattgtag
     3241 agcgagcgtg gactcatcat cgccagctcc gctaaccgct caaggctgat attccgtttg
     3301 agattctcct caatgaattg aacgactcgc tcgaaagacg ggttaccttt gctgaaaatt
     3361 tcacggctga cattgctgcc cagcatttcg agcagcttgg aagcgatgat ccccgcatag
     3421 tgctcttgga cccgaggcat cgactttgta tgttccgctt cgtcacaaac taacccgagt
     3481 agattgataa agccatcgag ttgctggaga ttgtgtcgcg cggcgaaacg gataccctcc
     3541 ctcggcttgt gccaattgtt gtcactgcac gcccgatcaa ggaccactga gggcaattta
     3601 acgataaatt tctcgcaatc ttctgaatag gtcaggtcgg cttggtcatc cggattgagc
     3661 agcaatagtt cgcccggcgc aaaatagtgc tcctggccat ggccacgcca caggcaatgg
     3721 cctttgagta ttatttgcag atgataacag gtctctaatc caggcgagat taccctcacg
     3781 ctaccgccgt agctgattcg acacagatcg aggcatccga agattctgtg gtgcagcctg
     3841 cctgccgggc gcccgccctt gggcaggcga atagagtgcg taccgacata ctggttaaca
     3901 taatcggaga ctgcataggg ctcggcgtgg acgaagatct gacttttctc gttcaataag
     3961 caaaaatcca tagttcacgg ttctcttatt ttaatgtggg ctgcttggtg tgatgtagaa
     4021 aggcgccaag tcgatgaaaa tgcatctcga cgtgatgcgt atacgggtta cccccattgc
     4081 cacgttgcgc catccttttt gcaatcagtg accacttttc caagcaaaaa taacgccaag
     4141 cagaacgaag acgttctttt taagaagcga gaacaccaga agttcgtgct gtcggggcat
     4201 ggggcgacga attggcggat aaaggggatc tgctggatat tacggccttt ttaaagaccg
     4261 taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga
     4321 atgctcatcc gtaattacgt atggcaatga aagacggtga gctggtgata tgggatagtg
     4381 ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg
     4441 aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg
     4501 gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtctcagcca
     4561 atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg
     4621 cccccgtttt caccatgcat gggaattagc ttgatctgac caacgaccgg tagcggagct
     4681 atccaacggc ggtataccag gaaaacacac agcaggtaca tcagaacagt accatgactg
     4741 aagaacaaat agttttttcc tgatccataa agcagaacgg cctgctccat gacaaatctg
     4801 gctccccaac taatgcccca tgcagccagc ataaccagca taaagtgcag tgtccggttt
     4861 gatagggata agtccagcct tgcaagaagc ggatacagga gtgcaaaaaa tggctatctc
     4921 tagtaaggcc taccccttag gctttatgca acagaaacaa taataatgga gtcatgacca
     4981 tgcctaggcc gcgggccgcg cgaattcaga aggagaatat accatgaacc aaatcgtgaa
     5041 gttcactgac gactctggcc tggcggttca agttactcca gacgatgttc gccgttatat
     5101 ctgtgagaac gctactgaaa aagaggtggg cctctttctg caactctgtc agactcaacg
     5161 tctgaatccg tttgtgaaag acgcttacct ggtgaaatac ggcggtgctc cagcttctat
     5221 gattacttcc tatcaagttt ttaaccgtcg cgcgtgtcgt gatgctaact atgatggtat
     5281 caaatctggt gtggttgttc tgcgtgacgg tgatgttgtg cataaacgtg gtgctgcgtg
     5341 ctacaaaaag gcgggtgagg agctcatcgg tggttgggcg gaagttcgct ttaaggatgg
     5401 ccgcgagact gcgtatgctg aggtggcgct cgacgactat tccaccggca aatctaattg
     5461 ggcgaaaatg ccgggtgtta tgatcgaaaa atgcgcgaag gctgctgctt ggcgcctcgc
     5521 gttcccggac acttttcagg gcatgtacgc tgcggaggaa atggatcaag cgcaacagcc
     5581 agaacaggtg cgcgctcagg cggagcaacc agtggatctc cagccaatcc gcgaactctt
     5641 caagccatat tgcgaacact tcggcatcac tccggctgag ggtatgactg ctgtttgtgg
     5701 tgcggtgggc gctgaaggca tgcactctat gaccgagcag caagctcgcc gtgctcgcgc
     5761 ttggatggag gaagaaatgg ctgcgccagc tgtggaagcg gagtatgagg ttgttgacga
     5821 gggcgaggtg ttttaaggat ccaggagaat acacatgcca attcaggtct taccgccaca
     5881 actggcgaac cagattgccg caggtgaggt ggtcgagcga cctgcgtcgg tagtcaaaga
     5941 actagtgaaa aacagcctcg atgcaggtgc gacgcgtatc gatattgata tcgaacgcgg
     6001 tggggcgaaa cttatccgca ttcgtgataa cggctgcggt atcaaaaaag atgagctggc
     6061 gctggcgctg gctcgtcatg ccaccagtaa aatcgcctct ctggacgatc tcgaagccat
     6121 tatcagcctg ggctttcgcg gtgaggcgct ggcgagtatc agttcggttt cccgcctgac
     6181 gctcacttca cgcaccgcag aacagcagga agcctggcag gcctatgccg aagggcgcga
     6241 tatgaacgtg acggtaaaac cggcggcgca tcctgtgggg acgacgctgg aggtgctgga
     6301 tctgttctac aacaccccgg cgcggcgcaa attcctgcgc accgagaaaa ccgaatttaa
     6361 ccacattgat gagatcatcc gccgcattgc gctggcgcgt ttcgacgtca cgatcaacct
     6421 gtcgcataac ggtaaaattg tgcgtcagta ccgcgcagtg ccggaaggcg ggcaaaaaga
     6481 acggcgctta ggcgcgattt gcggcaccgc ttttcttgaa caagcgctgg cgattgaatg
     6541 gcaacacggc gatctcacgc tacgcggctg ggtggccgat ccaaatcaca ccacgcccgc
     6601 actggcagaa attcagtatt gctacgtgaa cggtcgcatg atgcgcgatc gcctgatcaa
     6661 tcacgcgatc cgccaggcct gcgaagacaa actgggggcc gatcagcaac cggcatttgt
     6721 gttgtatctg gagatcgacc cacatcaggt ggacgtcaac gtgcaccccg ccaaacacga
     6781 agtgcgtttc catcagtcgc gtctggtgca tgattttatc tatcagggcg tgctgagcgt
     6841 gctacaacag caactggaaa cgccgctacc gctggacgat gaaccccaac ctgcaccgcg
     6901 ttccattccg gaaaaccgcg tggcggcggg gcgcaatcac tttgcagaac cggcagctcg
     6961 tgagccggta gctccgcgct acactcctgc gccagcatca ggcagtcgtc cggctgcccc
     7021 ctggccgaat gcgcagccag gctaccagaa acagcaaggt gaagtgtatc gccagctttt
     7081 gcaaacgccc gcgccgatgc aaaaattaaa agcgccggaa ccgcaggaac ctgcacttgc
     7141 ggcgaacagt cagagttttg gtcgggtact gactatcgtc cattccgact gtgcgttgct
     7201 ggagcgcgac ggcaacattt cacttttatc cttgccagtg gcagaacgtt ggctgcgtca
     7261 ggcacaattg acgccgggtg aagcgcccgt ttgcgcccag ccgctgctga ttccgttgcg
     7321 gctaaaagtt tctgccgaag aaaaatcggc attagaaaaa gcgcagtctg ccctggcgga
     7381 attgggtatt gatttccagt cagatgcaca gcatgtgacc atcagggcag tgcctttacc
     7441 cttacgccaa caaaatttac aaatcttgat tcctgaactg ataggctacc tggcgaagca
     7501 gtccgtattc gaacctggca atattgcgca gtggattgca cgaaatctga tgagcgaaca
     7561 tgcgcagtgg tcaatggcac aggccataac cctgctggcg gacgtggaac ggttatgtcc
     7621 gcaacttgtg aaaacgccgc cgggtggtct gttacaatct gttgatttac atccggcgat
     7681 aaaagccctg aaagatgagt gatctagagt cgacctgcag gcatgcaagc ttgcggccgc
     7741 gtcgtgactg ggaaaaccct ggcgactagt cttggactcc tgttgataga tccagtaatg
     7801 acctcagaac tccatctgga tttgttcaga acgctcggtt gccgccgggc gttttttatt
     7861 ggtgagaatc tatgccatag catttttatc cataagatta gcggatccta cctgacgctt
     7921 tttatcgcaa ctctctactg tttctccata cccgtttttt tgggaattcg agctctaagg
     7981 aggttaatga gagccctggt agtcatccgc ctgtcccgcg tcaccgatgc tacgacttca
     8041 ccggagcgtc agctggagtc ttgccagcag ctctgcgccc agcgcggctg ggacgtcgtc
     8101 ggggtagcgg aggatctgga cgtctccggg gcggtcgatc cgttcgaccg gaagcgcaga
     8161 ccgaacctgg cccggtggct agcgttcgag gagcaaccgt tcgacgtgat cgtggcgtac
     8221 cgggtagacc ggttgacccg atcgatccgg catctgcagc agctggtcca ctgggccgag
     8281 gaccacaaga agctggtcgt ctccgcgacc gaagcgcact tcgatacgac gacgccgttt
     8341 gcggcggtcg tcatcgcgct tatgggaacg gtggcgcaga tggaattaga agcgatcaaa
     8401 gagcggaacc gttcggctgc gcatttcaat atccgcgccg ggaaataccg aggatccctg
     8461 ccgccgtggg gatacctgcc tacgcgcgtg gacggggagt ggcggctggt gccggaccct
     8521 gtgcagcgag agcgcatcct cgaggtgtat caccgcgtcg tcgacaacca cgagccgctg
     8581 cacctggtgg cccacgacct gaaccggcgt ggtgtcctgt cgccgaagga ctacttcgcg
     8641 cagctgcaag gccgcgagcc gcagggccgg gagtggtcgg ctaccgcgct gaagcgatcg
     8701 atgatctccg aggcgatgct cgggtacgcg actctgaacg gtaagaccgt ccgagacgac
     8761 gacggagccc cgctggtgcg ggctgagccg atcctgaccc gtgagcagct ggaggcgctg
     8821 cgcgccgagc tcgtgaagac ctcccgggcg aagcccgcgg tgtctacccc gtcgctgctg
     8881 ctgcgggtgt tgttctgcgc ggtgtgcggg gagcccgcgt acaagttcgc cgggggagga
     8941 cgtaagcacc cgcgctaccg ctgccgctcg atggggttcc cgaagcactg cgggaacggc
     9001 acggtggcga tggccgagtg ggacgcgttc tgcgaggagc aggtgctgga tctgctcggg
     9061 gacgcggagc gtctggagaa agtctgggta gccggctcgg actccgcggt cgaactcgcg
     9121 gaggtgaacg cggagctggt ggacctgacg tcgctgatcg gctccccggc ctaccgggcc
     9181 ggctctccgc agcgagaagc actggatgtc cgtattgcgg cgctggccgc gcggcaagag
     9241 gagctggagg gtctagaggc tcgcccgtct ggctgggagt ggcgcgagac cgggcagcgg
     9301 ttcggggact ggtggcggga gcaggacacc gcggcaaaga acacctggct tcggtcgatg
     9361 aacgttcggc tgacgttcga cgtccgcggc gggctgactc gcacgatcga cttcggggat
     9421 ctgtaggagt acgagcagca tctcaggctc ggcagcgtgg tcgaacggct acacaccggg
     9481 atgtcgtagg ttaacaacta gcgtacgatc gactgccagg catcaaataa aacgaaaggc
     9541 tcagtcgaaa gactgggcct ttcgttttat gccatcatgg ccgcgggacg tcgggggggg
     9601 gggaaagcca cgttgtgtct caaaatctct gatgttacat tgcacaagat atgcaacaga
     9661 aactataaaa atacagagaa tgaaaagaaa cagatagatt ttttagttct ttaggcccgt
     9721 agtctgcaaa tccttttatg attttctatc aaacaaaaga ggaaaataga ccagttgcaa
     9781 tccaaacgag agtctaatag aatgaggtcg aaaagtaaat cgcgcgggtt tgttactgat
     9841 aaagcaggca agacctaaaa tgtgtaaagg gcaaagtgta tactttggcg tcacccctta
     9901 catattttag gtcttttttt attgtgcgta actaacttgc catcttcaaa caggagggct
     9961 ggaagaagca gaccgctaac acagtacata aaaaaggaga catgaacgat gaacatcaaa
    10021 aagtttgcaa aacaagcaac agtattaacc tttactaccg cactgctggc aggaggcgca
    10081 actcaagcgt ttgcgaaaga aacgaaccaa aagccatata aggaaacata cggcatttcc
    10141 catattacac gccatgatat gctgcaaatc cctgaacagc aaaaaaatga aaaatatcaa
    10201 gttcctgaat tcgattcgtc cacaattaaa aatatctctt ctgcaaaagg cctggacgtt
    10261 tgggacagct ggccattaca aaacgctgac ggcactgtcg caaactatca cggctaccac
    10321 atcgtctttg cattagccgg agatcctaaa aatgcggatg acacatcgat ttacatgttc
    10381 tatcaaaaag tcggcgaaac ttctattgac agctggaaaa acgctggccg cgtctttaaa
    10441 gacagcgaca aattcgatgc aaatgattct atcctaaaag accaaacaca agaatggtca
    10501 ggttcagcca catttacatc tgacggaaaa atccgtttat tctacactga tttctccggt
    10561 aaacattacg gcaaacaaac actgacaact gcacaagtta acgtatcagc atcagacagc
    10621 tctttgaaca tcaacggtgt agaggattat aaatcaatct ttgacggtga cggaaaaacg
    10681 tatcaaaatg tacagcagtt catcgatgaa ggcaactaca gctcaggcga caaccatacg
    10741 ctgagagatc ctcactacgt agaagataaa ggccacaaat acttagtatt tgaagcaaac
    10801 actggaactg aagatggcta ccaaggcgaa gaatctttat ttaacaaagc atactatggc
    10861 aaaagcacat cattcttccg tcaagaaagt caaaaacttc tgcaaagcga taaaaaacgc
    10921 acggctgagt tagcaaacgg cgctctcggt atgattgagc taaacgatga ttacacactg
    10981 aaaaaagtga tgaaaccgct gattgcatct aacacagtaa cagatgaaat tgaacgcgcg
    11041 aacgtcttta aaatgaacgg caaatggtac ctgttcactg actcccgcgg atcaaaaatg
    11101 acgattgacg gcattacgtc taacgatatt tacatgcttg gttatgtttc taattcttta
    11161 actggcccat acaagccgct gaacaaaact ggccttgtgt taaaaatgga tcttgatcct
    11221 aacgatgtaa cctttactta ctcacacttc gctgtacctc aagcgaaagg aaacaatgtc
    11281 gtgattacaa gctatatgac aaacagagga ttctacgcag acaaacaatc aacgtttgcg
    11341 ccaagcttcc tgctgaacat caaaggcaag aaaacatctg ttgtcaaaga cagcatcctt
    11401 gaacaaggac aattaacagt taacaaataa gcgtcagacc ccgtagaaaa gatcaaagga
    11461 tcttc
//
