LOCUS       sequence-405530-        2516 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 plasmid containing the promoter from gene 5902 from 
            Phaeodactylum tricornutum with AC overhangs used to build a level 1 
            construct..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, 
            Bielinski V, von Dassow P, Dupont CL, Federici F
  TITLE     Universal loop assembly: open, efficient and cross-kingdom DNA 
            fabrication.
  JOURNAL   Synth Biol (Oxf). 2020;5(1):ysaa001. doi: 10.1093/synbio/ysaa001. 
            Epub 2020 Feb 5.
  PUBMED    32161816
REFERENCE   2  (bases 1 to 2516)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2020"; volume: "5(1):ysaa001. doi"; pages: " 
            10.1093/synbio/ysaa001. Epub 2020 Feb 5"
COMMENT     Sequence Label: pL0_AC-HO
FEATURES             Location/Qualifiers
     source          1..2516
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     20..39
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1165..1956
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(2047..2516,1..119)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
       61 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
      121 acgccagcaa cgcggccttt ttacggttcc ttgccttttg ctggcctttt gctcacatgt
      181 tctttcctgc gttatcccct gattctgtgg gtggaagggc tcggagttgt ggtaatctat
      241 gtatcctggt ggtctcgcat tcaatcgagg gttctcaatc gtcgcttgcc cgtcatcgcc
      301 caatacaccc tggtttttga tggacacgcg tgtcgtgatg ggggtcttgt ggcagttggt
      361 ttgctttctc gagacggaag gagcaaatgc ctggctcgta cggattgggc tggccgcaac
      421 agctagtaca gccaaaagac gaagcttcgt aatattcctc attgcccact gcttgaaaac
      481 gctcttgact gagactaaca gtaagatgca gttatctctc tcagtcaacg tatatcctca
      541 tcaggtcccg actattttgt cgtcatcgtt aaccggacga ctatgctgtg aagtaaaatc
      601 gccaagctga ctcatgaaaa gaacgcaatc cagtcttgta cgttcgtgaa tgagaggtat
      661 cgacgtcaga cgggattacc gggaaggtca atcatgagag tagcgcgtgc ttgtcgtctc
      721 cggagacctg agacgagacg agacagcctg agaatggatg cgagtaatga gctcggcaca
      781 aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg gagagccgtt
      841 gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc aactaagctg
      901 ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa cgatgacaga
      961 gcgttgctgc ctgtgatcac cgctcctcta gagtcgactt gcaggcatgc aagcttagca
     1021 agcgaaccgg aattgccagc tggggcgccc tctggtaagg ttgggaagcc ctgcaaagta
     1081 aactggatgg ctttcttgcc gccaaggatc tgatggcgca ggggatcaat atctgatcaa
     1141 gagacaggat gaggatcgtt tcgcatgagg gaagcggtga tcgccgaagt atcgactcaa
     1201 ctatcagagg tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat
     1261 ttgtacggct ccgcagtgga tggcggcctg aagccacaca gtgatattga tttgctggtt
     1321 acggtgaccg taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa
     1381 acttcggctt cccctggaga gagtgagatt ctccgcgctg tagaagtcac cattgttgtg
     1441 cacgacgaca tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg
     1501 cagcgcaatg acattcttgc tggtatcttc gagccagcca cgatcgacat tgatctggct
     1561 atcttgctga caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa
     1621 ctctttgatc cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta
     1681 tggaactcgc cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc
     1741 atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca
     1801 atggagcgcc tgccggccca gtatcagccc gtcatacttg aagctagaca ggcttatctt
     1861 ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgtccactac
     1921 gtgaaaggcg agatcaccaa ggtagtcggc aaataataat cacctagaat gcatgaccaa
     1981 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     2041 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     2101 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     2161 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     2221 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     2281 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     2341 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     2401 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     2461 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagc
//
