LOCUS       sequence-405543-        2428 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 plasmid containing the promoter from gene 22357 from 
            Phaeodactylum tricornutum with AC overhangs used to build a level 1 
            construct..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, 
            Bielinski V, von Dassow P, Dupont CL, Federici F
  TITLE     Universal loop assembly: open, efficient and cross-kingdom DNA 
            fabrication.
  JOURNAL   Synth Biol (Oxf). 2020;5(1):ysaa001. doi: 10.1093/synbio/ysaa001. 
            Epub 2020 Feb 5.
  PUBMED    32161816
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2020"; volume: "5(1):ysaa001. doi"; pages: " 
            10.1093/synbio/ysaa001. Epub 2020 Feb 5"
COMMENT     Sequence Label: pL0_AC-glna
FEATURES             Location/Qualifiers
     source          1..2428
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      272..860
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     761..780
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(1818..2428,1..181)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat
       61 acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga
      121 tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag tcggcaaata
      181 ataatcacct agaatgcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
      241 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
      301 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
      361 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
      421 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
      481 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
      541 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
      601 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
      661 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
      721 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
      781 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
      841 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc cttgcctttt
      901 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg ggtggaaggg
      961 ctcggagttg tggtaatcta tgtatcctgg tggtctcgca ttgcttggaa gtttgggatg
     1021 tggtgtgtat atgagagagt atacgagtgt gtgtgttagg tagggaggca gaacgcggac
     1081 acacgcttta tacgttgtga tcgatcagga tatcgtggtt gctgcctgca cgagggtgcg
     1141 tcgtgttcga atttggtgtg tgtgttttgc ttggtgcaac tggggttcct tcggggggac
     1201 tggtgttgtc tgtgtgtgac ttactgtaat tttgggggcg attactacgg tcggttgagt
     1261 cgggttcgga tctgagggtc cgttccaaaa gatccattcc ccaatttctc tctcgcgtcg
     1321 gtggatcttc tgtcgccgta gaaccatact gtacgttctc tatccgtcta ctcctgagac
     1381 ctgagacgag acgagacagc ctgagaatgg atgcgagtaa tgagctcggc acaaaatcac
     1441 cactcgatac aggcagccca tcagtccggg acggcgtcag cgggagagcc gttgtaaggc
     1501 ggcagacttt gctcatgtta ccgatgctat tcggaagaac ggcaactaag ctgccgggtt
     1561 tgaaacacgg atgatctcgc ggagggtagc atgttgattg taacgatgac agagcgttgc
     1621 tgcctgtgat caccgctcct ctagagtcga cttgcaggca tgcaagctta gcaagcgaac
     1681 cggaattgcc agctggggcg ccctctggta aggttgggaa gccctgcaaa gtaaactgga
     1741 tggctttctt gccgccaagg atctgatggc gcaggggatc aatatctgat caagagacag
     1801 gatgaggatc gtttcgcatg agggaagcgg tgatcgccga agtatcgact caactatcag
     1861 aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg
     1921 gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga
     1981 ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg
     2041 cttcccctgg agagagtgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg
     2101 acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca
     2161 atgacattct tgctggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc
     2221 tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg
     2281 atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact
     2341 cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt
     2401 acagcgcagt aaccggcaaa atcgcgcc
//
