LOCUS       sequence-405623-        2284 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 plasmid containing a terminator sequence from gene 51305 
            from Phaeodactylum tricornutum with EF overhangs used to build a 
            level 1 construct..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, 
            Bielinski V, von Dassow P, Dupont CL, Federici F
  TITLE     Universal loop assembly: open, efficient and cross-kingdom DNA 
            fabrication.
  JOURNAL   Synth Biol (Oxf). 2020;5(1):ysaa001. doi: 10.1093/synbio/ysaa001. 
            Epub 2020 Feb 5.
  PUBMED    32161816
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2020"; volume: "5(1):ysaa001. doi"; pages: " 
            10.1093/synbio/ysaa001. Epub 2020 Feb 5"
COMMENT     Sequence Label: pL0_EF-BCA
FEATURES             Location/Qualifiers
     source          1..2284
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      251..839
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     740..759
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(1653..2284,1..160)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ggcaatggag cgcctgccgg cccagtatca gcccgtcata cttgaagcta gacaggctta
       61 tcttggacaa gaagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgtcca
      121 ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa taatcaccta gaatgcatga
      181 ccaaaatccc ttaacgtgag ttttcgtacc actgagcgtc agaccccgta gaaaagatca
      241 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
      301 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
      361 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
      421 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
      481 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
      541 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
      601 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
      661 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
      721 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
      781 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
      841 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
      901 tctttcctgc gttatcccct gattctgtgg gtggaagggc tcggagttgt ggtaatctat
      961 gtatcctggt ggtctcaagc ggtgtcaacc cgcatagtta aactacgtgg acgtcactcc
     1021 atagtcataa tcagttattt atgtcgggtg aaatcagatc aaggttacat cggtatcgac
     1081 agcgtaacag ttcttatagg cgataaacca acattgatcc tagtcgcttg ctcgtcaata
     1141 ctaccttcgg aaaaaaatgg tattgattat agcataaatt aattacacag aatcattttc
     1201 caagtaagct gagacctgag acgagacgag acagcctgag aatggatgcg agtaatgagc
     1261 tcggcacaaa atcaccactc gatacaggca gcccatcagt ccgggacggc gtcagcggga
     1321 gagccgttgt aaggcggcag actttgctca tgttaccgat gctattcgga agaacggcaa
     1381 ctaagctgcc gggtttgaaa cacggatgat ctcgcggagg gtagcatgtt gattgtaacg
     1441 atgacagagc gttgctgcct gtgatcaccg ctcctctaga gtcgacttgc aggcatgcaa
     1501 gcttagcaag cgaaccggaa ttgccagctg gggcgccctc tggtaaggtt gggaagccct
     1561 gcaaagtaaa ctggatggct ttcttgccgc caaggatctg atggcgcagg ggatcaatat
     1621 ctgatcaaga gacaggatga ggatcgtttc gcatgaggga agcggtgatc gccgaagtat
     1681 cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg
     1741 ccgtacattt gtacggctcc gcagttgatg gcggcctgaa gccacacagt gatattgatt
     1801 tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc
     1861 ttttggaaac ttcggcttcc cctggagaga gtgagattct ccgcgctgta gaagtcacca
     1921 ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg
     1981 gagaatggca gcgcaatgac attcttgctg gtatcttcga gccagccacg atcgacattg
     2041 atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg
     2101 cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct
     2161 taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt
     2221 tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg
     2281 actg
//
