LOCUS       sequence-405676-        2495 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 plasmid containing a terminator sequence from gene 54499 
            from Phaeodactylum tricornutum with EF overhangs used to build a 
            level 1 construct..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, 
            Bielinski V, von Dassow P, Dupont CL, Federici F
  TITLE     Universal loop assembly: open, efficient and cross-kingdom DNA 
            fabrication.
  JOURNAL   Synth Biol (Oxf). 2020;5(1):ysaa001. doi: 10.1093/synbio/ysaa001. 
            Epub 2020 Feb 5.
  PUBMED    32161816
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2020"; volume: "5(1):ysaa001. doi"; pages: " 
            10.1093/synbio/ysaa001. Epub 2020 Feb 5"
COMMENT     Sequence Label: pL0_EF-OEE3
FEATURES             Location/Qualifiers
     source          1..2495
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      763..1351
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1252..1271
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(2376..2495,1..672)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ggcctgaagc cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa
       61 acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagt
      121 gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt
      181 tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgctggt
      241 atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa
      301 catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag
      361 gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct
      421 ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc
      481 aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat
      541 cagcccgtca tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc
      601 tcgcgcgcag atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta
      661 gtcggcaaat aataatcacc tagaatgcat gaccaaaatc ccttaacgtg agttttcgtt
      721 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
      781 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
      841 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
      901 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
      961 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     1021 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     1081 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     1141 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     1201 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     1261 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     1321 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     1381 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     1441 gggtggaagg gctcggagtt gtggtaatct atgtatcctg gtggtctcga gcgaccgccg
     1501 acatcagtat taacgccaag cgttttttcg acaagctgag atttcgggcc aaaatcttcg
     1561 actggttcat ccttagatca ttgacagtag gagaaacatg ggcgaggctc cttctccaaa
     1621 tcgactcaaa ccttgagggc acatgcccat tcaaattgct attcaaggca aagcttggag
     1681 ggcccagggg tttctatttg gtagagatct gtcgtcaacc cggcgtacta ttcatgctgg
     1741 cattcaaatc ttgctgacat gaagcatcgt ccactaatcc aatgcctacc gttccaacga
     1801 atgcctagat tttcaataat ttcatccaca tcgaagccta tttctacaaa gacggcccag
     1861 acccattaaa ctctttcaaa ctgactttca atttatgtca atactagtcc aggagtcaga
     1921 tgcaaatcaa gctgagacct gagacgagac gagacagcct gagaatggat gcgagtaatg
     1981 agctcggcac aaaatcacca ctcgatacag gcagcccatc agtccgggac ggcgtcagcg
     2041 ggagagccgt tgtaaggcgg cagactttgc tcatgttacc gatgctattc ggaagaacgg
     2101 caactaagct gccgggtttg aaacacggat gatctcgcgg agggtagcat gttgattgta
     2161 acgatgacag agcgttgctg cctgtgatca ccgctcctct agagtcgact tgcaggcatg
     2221 caagcttagc aagcgaaccg gaattgccag ctggggcgcc ctctggtaag gttgggaagc
     2281 cctgcaaagt aaactggatg gctttcttgc cgccaaggat ctgatggcgc aggggatcaa
     2341 tatctgatca agagacagga tgaggatcgt ttcgcatgag ggaagcggtg atcgccgaag
     2401 tatcgactca actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc
     2461 tggccgtaca tttgtacggc tccgcagtgg atggc
//
