LOCUS       sequence-405678-        2497 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 plasmid containing a terminator sequence from gene 39236 
            from Phaeodactylum tricornutum with EF overhangs used to build a 
            level 1 construct..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, 
            Bielinski V, von Dassow P, Dupont CL, Federici F
  TITLE     Universal loop assembly: open, efficient and cross-kingdom DNA 
            fabrication.
  JOURNAL   Synth Biol (Oxf). 2020;5(1):ysaa001. doi: 10.1093/synbio/ysaa001. 
            Epub 2020 Feb 5.
  PUBMED    32161816
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2020"; volume: "5(1):ysaa001. doi"; pages: " 
            10.1093/synbio/ysaa001. Epub 2020 Feb 5"
COMMENT     Sequence Label: pL0_EF-CaMK2
FEATURES             Location/Qualifiers
     source          1..2497
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      267..855
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     756..775
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(1882..2497,1..176)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg
       61 aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt
      121 tggaagaatt tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataataat
      181 cacctagaat gcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
      241 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
      301 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
      361 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
      421 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
      481 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
      541 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
      601 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
      661 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
      721 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
      781 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
      841 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttccttgc cttttgctgg
      901 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtgggtgg aagggctcgg
      961 agttgtggta atctatgtat cctggtggtc tcgagcgtag gatcgcattc ttgaagctca
     1021 gatcgacgtt gtgagactgg aaccatttgg ctgaccgtga atgattttgt gatacgtgtc
     1081 tgtacactat ttacagtaca gaaattgctt cacagttcta tttaatggaa agttgtccta
     1141 atgtcagatc tcactattaa ctgttacagt tagctggcct cgaatctaca ttgaaaagat
     1201 ttacttgttc tacatgtcgc gcattcacag tcaacccaac ctaaagcaaa tcggtatggc
     1261 aacaataaac tacctgacac aatacgattt tcttaaagca ctttcaataa caggaaaagg
     1321 tccgttactt tttcgattgc tgtcaaagta tatatgcgcg ccatcttgtg tgtggcgaat
     1381 ccgtttcaaa accccgggcc cgtccatggg cagagcgatg gctaatgaac acacaagcgg
     1441 agacctgaga cgagacgaga cagcctgaga atggatgcga gtaatgagct cggcacaaaa
     1501 tcaccactcg atacaggcag cccatcagtc cgggacggcg tcagcgggag agccgttgta
     1561 aggcggcaga ctttgctcat gttaccgatg ctattcggaa gaacggcaac taagctgccg
     1621 ggtttgaaac acggatgatc tcgcggaggg tagcatgttg attgtaacga tgacagagcg
     1681 ttgctgcctg tgatcaccgc tcctctagag tcgacttgca ggcatgcaag cttagcaagc
     1741 gaaccggaat tgccagctgg ggcgccctct ggtaaggttg ggaagccctg caaagtaaac
     1801 tggatggctt tcttgccgcc aaggatctga tggcgcaggg gatcaatatc tgatcaagag
     1861 acaggatgag gatcgtttcg catgagggaa gcggtgatcg ccgaagtatc gactcaacta
     1921 tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg
     1981 tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg
     2041 gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact
     2101 tcggcttccc ctggagagag tgagattctc cgcgctgtag aagtcaccat tgttgtgcac
     2161 gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag
     2221 cgcaatgaca ttcttgctgg tatcttcgag ccagccacga tcgacattga tctggctatc
     2281 ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc
     2341 tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg
     2401 aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt
     2461 tggtacagcg cagtaaccgg caaaatcgcg ccgaagg
//
