LOCUS       sequence-405715-        9697 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of full length Sox13.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9697)
  AUTHORS   Price RM, Budzynski MA, Shen J, Mitchell JE, Kwan JZJ, Teves SS
  TITLE     Heat shock transcription factors demonstrate a distinct mode of 
            interaction with mitotic chromosomes.
  JOURNAL   Nucleic Acids Res. 2023 Apr 28:gkad304. doi: 10.1093/nar/gkad304.
  PUBMED    37114996
REFERENCE   2  (bases 1 to 9697)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2023 Apr 28:gkad304. doi: 10.1093/nar/gkad304."
COMMENT     Sequence Label: Halo-Sox13
FEATURES             Location/Qualifiers
     source          1..9697
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     5..24
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   798..832
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(1323..1339)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1323..1339)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1336..1358)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1347..1363
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1371..1377,1378..1395,1396..1401))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1416..1437
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1554..1571)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1725..2313)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1805..1824)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2484..3275,3276..3344))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3107..3126
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3345..3449)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3475..3930)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3612..3633)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3824..3843
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4057..4079
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4071..4088
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4072..4088
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   4218..4280
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        4733..4944
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             4957..5225
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             5257..5322
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             5332..6222
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     CDS             6235..6255
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
     misc_feature    8114..8686
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8281..8298)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8508..8527
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             8695..9489
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLAATGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8749..8768)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9359..9378
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(9549..9569)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    join(9611..9697,1..35)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9648..9667)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggaattg actcaaatga
       61 tgtcaattag tctatcagaa gctcatctgg tctcccttcc gggggacaag acatccctgt
      121 ttaatattta aacagcagtg ttcccaaact gggttcttat atcccttgct ctggtcaacc
      181 aggttgcagg gtttcctgtc ctcacaggaa cgaagtccct aaagaaacag tggcagccag
      241 gtttagcccc ggaattgact ggattccttt tttagggccc attggtatgg ctttttcccc
      301 gtatcccccc aggtgtctgc aggctcaaag agcagcgaga agcgttcaga ggaaagcgat
      361 cccgtgccac cttccccgtg cccgggctgt ccccgcacgc tgccggctcg gggatgcggg
      421 gggagcgccg gaccggagcg gagccccggg cggctcgctg ctgcccccta gcgggggagg
      481 gacgtaatta catccctggg ggctttgggg gggggctgtc cctgatatct ataacaagaa
      541 aatatatata taataagtta tcacgtaagt agaacatgaa ataacaatat aattatcgta
      601 tgagttaaat cttaaaagtc acgtaaaaga taatcatgcg tcattttgac tcacgcggtc
      661 gttatagttc aaaatcagtg acacttaccg cattgacaag cacgcctcac gggagctcca
      721 agcggcgact gagatgtcct aaatgcacag cgacggattc gcgctattta gaaagagaga
      781 gcaatatttc aagaatgcat gcgtcaattt tacgcagact atctttctag ggttaatcta
      841 gctgcatcag gatcatatcg tcgggtcttt tttccggctc agtcatcgcc caagctggcg
      901 ctatctgggc atcggggagg aagaagcccg tgccttttcc cgcgaggttg aagcggcatg
      961 gaaagagttt gccgaggatg actgctgctg cattgacgtt gagcgaaaac gcacgtttac
     1021 catgatgatt cgggaaggtg tggccatgca cgcctttaac ggtgaactgt tcgttcaggc
     1081 cacctgggat accagttcgt cgcggctttt ccggacacag ttccggatgg tcagcccgaa
     1141 gcgcatcagc aacccgaaca ataccggcga cagccggaac tgccgtgccg gtgtgcagat
     1201 taatgacagc ggtgcggcgc tgggatatta cgtcagcgag gacgggtatc ctggctggat
     1261 gccgcagaaa tggacatgga taccccgtga gttacccggc gggcgcgctt ggcgtaatca
     1321 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     1381 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     1441 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     1501 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     1561 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     1621 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     1681 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     1741 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     1801 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     1861 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     1921 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     1981 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     2041 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     2101 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     2161 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     2221 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     2281 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     2341 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     2401 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     2461 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     2521 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     2581 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     2641 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     2701 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     2761 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     2821 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     2881 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     2941 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     3001 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     3061 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     3121 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     3181 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     3241 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     3301 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     3361 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     3421 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctaaattg
     3481 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta
     3541 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
     3601 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
     3661 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
     3721 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
     3781 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
     3841 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
     3901 ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc attcaggctg cgcaactgtt
     3961 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
     4021 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
     4081 cggccagtga gcgcgcctcg ttcattcacg tttttgaacc cgtggaggac gggcagactc
     4141 gcggtgcaaa tgtgttttac agcgtgatgg agcagatgaa gatgctcgac acgctgcaga
     4201 acacgcagct agattaaccc tagaaagata atcatattgt gacgtacgtt aaagataatc
     4261 atgtgtaaaa ttgacgcatg tgttttatcg gtctgtatat cgaggtttat ttattaattt
     4321 gaatagatat taagttttat tatatttaca cttacatact aataataaat tcaacaaaca
     4381 atttatttat gtttatttat ttattaaaaa aaacaaaaac tcaaaatttc ttctataaag
     4441 taacaaaact tttatgaggg acagcccccc cccaaagccc ccagggatgt aattacgtcc
     4501 ctcccccgct agggggcagc agcgagccgc ccggggctcc gctccggtcc ggcgctcccc
     4561 ccgcatcccc gagccggcag cgtgcgggga cagcccgggc acggggaagg tggcacggga
     4621 tcgctttcct ctgaacgctt ctcgctgctc tttgagcctg cagacacctg gggggatacg
     4681 gggaaaaggc ctccacggcc aaggatctgc gatcgctccg gtgcccgtca gtgggcagag
     4741 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg aacgggtgcc
     4801 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt
     4861 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc
     4921 aacgggtttg ccgccagaac acagctgaag cttcgagggg ctcgcatctc tccttcacgc
     4981 gcccgccgcc ctacctgagg ccgccatcca cgccggttga gtcgcgttct gccgcctccc
     5041 gcctgtggtg cctcctgaac tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga
     5101 ccgggccttt gtccggcgct cccttggagc ctacctagac tcagccggct ctccacgctt
     5161 tgcctgaccc tgcttgctca actctacgtc tttgtttcgt tttctgttct gcgccgttac
     5221 agatccaagc tgtgaccggc gcctactcta gccatggact acaaagacca tgacggtgat
     5281 tataaagatc atgacatcga ttacaaggat gacgatgaca agcttggctc catggcagaa
     5341 atcggtactg gctttccatt cgacccccat tatgtggaag tcctgggcga gcgcatgcac
     5401 tacgtcgatg ttggtccgcg cgatggcacc cctgtgctgt tcctgcacgg taacccgacc
     5461 tcctcctacg tgtggcgcaa catcatcccg catgttgcac cgacccatcg ctgcattgct
     5521 ccagacctga tcggtatggg caaatccgac aaaccagacc tgggttattt cttcgacgac
     5581 cacgtccgct tcatggatgc cttcatcgaa gccctgggtc tggaagaggt cgtcctggtc
     5641 attcacgact ggggctccgc tctgggtttc cactgggcca agcgcaatcc agagcgcgtc
     5701 aaaggtattg catttatgga gttcatccgc cctatcccga cctgggacga atggccagaa
     5761 tttgcccgcg agaccttcca ggccttccgc accaccgacg tcggccgcaa gctgatcatc
     5821 gatcagaacg tttttatcga gggtacgctg ccgatgggtg tcgtccgccc gctgactgaa
     5881 gtcgagatgg accattaccg cgagccgttc ctgaatcctg ttgaccgcga gccactgtgg
     5941 cgcttcccaa acgagctgcc aatcgccggt gagccagcga acatcgtcgc gctggtcgaa
     6001 gaatacatgg actggctgca ccagtcccct gtcccgaagc tgctgttctg gggcacccca
     6061 ggcgttctga tcccaccggc cgaagccgct cgcctggcca aaagcctgcc taactgcaag
     6121 gctgtggaca tcggcccggg tctgaatctg ctgcaagaag acaacccgga cctgatcggc
     6181 agcgagatcg cgcgctggct gtcgacgctc gagatttccg gcgagccaac cactgaggat
     6241 ctgtactttc agagcgataa ctctagatcc atgcagagcc ccgtctctgt gcagctggcc
     6301 ccagacagcg ctagcaccat ggtgaactgc accatcaagt ctgaggagaa gaaggagcct
     6361 tgccacgagg ctccccaagg tgcagcccct gcagtggaga cccagcctgg agacccggcc
     6421 ctggcctctc aggatgccac caacgctaaa gctcctcctc aggactgtgc ctcaccagag
     6481 agcagtgggt ccccagaacc caagagacca gcagcttccg aggctgcttc gggaagccag
     6541 gagcgcctgg acttcaaccg caacttacag gaggttgtgc cagccattga gaagctgctg
     6601 tccagtgact ggaaggagag gtttctggga agaagcaatg tggaagctaa ggatgtcaaa
     6661 gggacccaag agagcctggc tgagaaggaa ctccagcttt tggtcatgat ccatcagctc
     6721 tctgcccttc gggaccagct ccttacggcc cactctgagc agaagaacat ggccgccatg
     6781 ctgtttgaga agcagcagca acagatggaa ctggccaggc agcagcagga gcagatcgcc
     6841 aagcagcagc agcagctgat tcaacagcag cacaagatca acctcctgca gcagcagatc
     6901 cagcaggtta acatgcctta cgtcatgatc ccagccttcc ccccaagcca ccagcctttg
     6961 cctgtcactc ctgactccca gctggcttta cctattcagc ccattccctg caagccagtg
     7021 gaatacccac tgcagctgct gcacagcccg cctgcccctg tggtgaagag gtcgggggtg
     7081 gccgcccacc accccctgca ggagccccca caaccactga acctcacagc caagcccaag
     7141 gtacctgagc tgcccaacac ctccagctct cccagcctga agatgaacag ttgtgggcct
     7201 cgccccgcca gccatggggc tcccacaagg gacctgcaat ccagcccccc cagcctgcct
     7261 ctgggcttcc tcggtgaagg ggacgccgtc accaaagcca tccaggatgc ccggcagctg
     7321 ctgcacagcc acagtggggc tttggaaaac tcccccaata ccccattccg gaaggacctc
     7381 atcagcctgg actcctcccc ggccaaggag cggctggagg agagctgtgt gcatcccctg
     7441 gaggaagcca tgctgagctg tgacatggat ggctcccgtc atttttccga gtctcggaac
     7501 agcagccaca tcaagagacc catgaatgcc ttcatggtgt gggccaagga tgaacggagg
     7561 aaaattctcc aggccttccc agacatgcat aactccagca tcagcaagat ccttggttct
     7621 cgctggaagt ccatgaccaa ccaggagaaa cagccgtact acgaggagca ggcccgtctg
     7681 agccggcagc acctggagaa ataccctgac tataagtaca agccaaggcc taagcgtacc
     7741 tgtgtcgtgg agggccggcg gctgcgagtg ggcgagtaca aggccttgat gaggacacgg
     7801 cgccagggtg ctcgccaaag ctacaccatc cccccgcagg ctggccaggc acaggtgagc
     7861 tcagatatcc tgttccctcg ggcagcgggc ttgcccctgg cacgtccact ggtggagcac
     7921 tatgaccccc agggcctgga ccccaacatg cctgtcatca tcaatacctg cagtctccgg
     7981 gaggaagggg agggcacaga tgacaggcac tcggtggccg atggagagat gtaccggtac
     8041 agcgaggacg aggactcaga gggtgacgag aagagtgatg aggagctggt ggtactcaca
     8101 gactgaggcc gcgcccctct ccctcccccc cccctaacgt tactggccga agccgcttgg
     8161 aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca
     8221 atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc
     8281 ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag
     8341 cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg
     8401 gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac
     8461 aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa
     8521 gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc
     8581 tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc
     8641 ccccgaacca cggggacgtg gttttccttt gaaaaacacg ataatacctc cggaatgatt
     8701 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
     8761 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
     8821 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac
     8881 gaggcagcgc ggctatcgtg gctggccgcg acgggcgttc cttgcgcagc tgtgctcgac
     8941 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
     9001 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
     9061 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
     9121 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
     9181 caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag
     9241 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
     9301 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
     9361 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
     9421 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
     9481 ttcttctgag tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     9541 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     9601 gcgttaacta aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     9661 aaatttcaca aataaagcat ttttttcact gcattct
//
